Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,680,400 A→T 10.5% V417D (GTT→GAT)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,680,4000AT10.5% 529.0 / 12.7 241V417D (GTT→GAT) EO946_RS13875LysM peptidoglycan‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (94/101);  new base T (9/16);  total (111/130)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

CTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACG  >  NZ_CP034943/2680266‑2680539
                                                                                                                                      |                                                                                                                                           
cttcttTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGc                                                                                                                                        <  2:1655025/140‑1 (MQ=255)
cttcttTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGc                                                                                                                                        <  1:4542425/140‑1 (MQ=255)
 ttctttatATTCATGAAGTAGTGCAGGCTCTGCCTGCTCCGATTGAGCCTCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCt                                                                                                                                       <  1:2553917/140‑1 (MQ=18)
          ttCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAg                                                                                                                              >  1:560817/1‑140 (MQ=255)
          ttCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGAGCTTCTTGACGGATTGCGGGCTCAACGTGCTCCGGTTGAg                                                                                                                              >  2:2113738/1‑140 (MQ=255)
           tCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGc                                                                                                                             <  1:2601011/140‑1 (MQ=255)
           tCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGc                                                                                                                             <  1:16386/140‑1 (MQ=255)
            cATGACGTAGTGCAGGCTCGGCATGCTCCGCTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGcc                                                                                                                            <  1:2440950/140‑1 (MQ=255)
                  gTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGcttct                                                                                                                      >  2:4396363/1‑140 (MQ=255)
                   tAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCTGTTGAGCCGCTTCTTTCGCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGcttctt                                                                                                                     <  2:411746/140‑1 (MQ=255)
                    aGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCttcttc                                                                                                                    >  2:713366/1‑140 (MQ=255)
                      tGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTg                                                                                                                  <  2:284056/140‑1 (MQ=255)
                      tGCAGGCTCCGCCTGCTCCGCTTGAGCCGATTCTTCCGCTTCATGACGGAGTGCGGGCTCAGAGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTg                                                                                                                  <  2:940594/140‑1 (MQ=17)
                      ggCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTg                                                                                                                  <  2:4642542/139‑1 (MQ=255)
                           gCTCGGCATGCTCCGATTGAGCCGCTTCTTCGGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCGGCTTCTTTCTCTTCTTGACGGACTGCGGGCTCACCGTGATCCGGTTGAGCGGCTTCTTGTGcttct                                                                                                             >  2:2426091/1‑140 (MQ=17)
                           gCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                                                                             >  2:4812673/1‑140 (MQ=255)
                           gCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                                                                             >  1:74719/1‑140 (MQ=255)
                               ggCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAc                                                                                                         <  2:4566712/140‑1 (MQ=255)
                                gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGACg                                                                                                        >  2:1186821/1‑140 (MQ=255)
                                gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCGTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTATTCTTCTTGACg                                                                                                        >  2:41921/1‑140 (MQ=255)
                                 cATGCTCCGAGTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  1:4421403/140‑1 (MQ=255)
                                 cATGCTCCGAGTGAGCCGCGTCTTCCTCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  1:599881/140‑1 (MQ=255)
                                     cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   >  1:1068553/1‑140 (MQ=255)
                                     cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCGTCTTGTGCTTCTTGACGGAGTg                                                                                                   >  1:2291883/1‑140 (MQ=255)
                                     cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTATCTTCTTTACGGAGTGCCGGCTCAACGTGCGCCGGTTGATCCGCTTCGGCAGCGTCGTGTCGGAGTg                                                                                                   >  1:2185563/1‑140 (MQ=17)
                                     cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTGTCTCTTCTTGACGGAGTTCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCGTCTTGACGGCGTg                                                                                                   >  1:3934280/1‑140 (MQ=255)
                                     cTCCGATTGAGCCGCTTCTTCCGCGTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  2:1068553/140‑1 (MQ=255)
                                     cTCCGATTGAGCAGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCTGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  1:1966374/140‑1 (MQ=255)
                                     cTCAGATTGAGCCGATTCTTCCGCTTCATGACGGAGTGCGTGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCTGTTGAGCCGCTTATTCTGCTTCTTGACGGAGTg                                                                                                   <  1:3719467/140‑1 (MQ=17)
                                       ccGATTGAGCCGCTTCTTCCGCTTCATGACGGCGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTGCTTGACGGAGTGCGGGCGCATCGGGCTCCGGTTGAGCGGCTTCTTCTGCTTCTTGACGGAGTGCg                                                                                                 >  2:1268008/1‑140 (MQ=17)
                                          aTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAAc                                                                                                                                            <  2:1820444/94‑1 (MQ=255)
                                          aTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCGGCTTCTTATTCTTCTTGACGGAGTGcctgc                                                                                              >  2:3213054/1‑136 (MQ=255)
                                             gAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCa                                                                                           <  1:2417991/140‑1 (MQ=255)
                                              aGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAg                                                                                          <  2:560817/140‑1 (MQ=255)
                                                ccGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTGTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        >  1:4523850/1‑140 (MQ=255)
                                                ccGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        >  2:342965/1‑140 (MQ=255)
                                                ccGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        <  2:3087978/140‑1 (MQ=255)
                                                ccGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGGTCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        >  2:1304206/1‑140 (MQ=14)
                                                   cttcttCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTGTCTTGTTGAGGGAGTGGGGGCTCAACGTGCTCCGGTTGAGCCGGGGCTTCTGGTTGTTTACGGAGTGCGGGCTCAGCGTGc                                                                                     >  2:668200/1‑140 (MQ=17)
                                                   cttcttCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCATGCTCTTCTTGAAGGAGTGCGGGCTCACCGAGCACCGGATGAGCCGCATCTTCTGGATCATGACGGAGAGCGCGCTCAGCGTGc                                                                                     >  2:2605210/1‑140 (MQ=17)
                                                    ttcttcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCt                                                                                    <  1:4294301/140‑1 (MQ=255)
                                                    ttcttcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCt                                                                                    <  1:1226203/140‑1 (MQ=255)
                                                    ttcttcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCt                                                                                    <  1:4916770/140‑1 (MQ=255)
                                                      cttcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCGTTCTCGTCTTGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTcc                                                                                  <  2:4670994/140‑1 (MQ=255)
                                                              tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCCGGCTCAGCCTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGc                                                                          <  1:5063513/137‑1 (MQ=14)
                                                                 tGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCCTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGc                                                                       <  1:2870067/140‑1 (MQ=255)
                                                                 tGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGc                                                                       <  1:1101215/140‑1 (MQ=255)
                                                                         tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTg                                                               >  2:4019238/1‑140 (MQ=255)
                                                                           cGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTGTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGGTCCGGTTGAGCCGCTtcttcttct                                                             >  1:118026/1‑140 (MQ=255)
                                                                           cGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGct                                                             >  1:1728981/1‑140 (MQ=255)
                                                                              gCTCAGCGTGGTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                          <  2:3592740/140‑1 (MQ=255)
                                                                              gCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                          <  1:3642104/140‑1 (MQ=255)
                                                                                 cAGCGTGCTCCGGTTGAGCCGCTTCTTTGTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCGGCTTCTTAGGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCGGCTTCTTGTTGTTCTTGa                                                       >  1:3590389/1‑140 (MQ=14)
                                                                                 cAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGa                                                       >  1:2914002/1‑140 (MQ=255)
                                                                                 cAGCGTGCTCCGGTTGAGCCGCTTCGTTGTCTTCGTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGTGCCGCTTCTTGGTCGTCTTGACGGAGTGCGGGCTCAGCGTGCTGCGGTTGAGCCGCGTCGTCTGCTTCTTGa                                                       >  1:4680596/1‑140 (MQ=14)
                                                                                  aGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAc                                                      >  2:3362195/1‑140 (MQ=255)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTGTTTGTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTGTGCTTCTTTACGGAGTGCGGGCTCAGCGTGCTACGGTTTTGCCGCTTCTTCTGCTTCTTTACg                                                     >  1:3432509/1‑140 (MQ=17)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGATTGCGGGCTCAACGTGCTCCCGTTGAGCCGCTTCGTGTGCTTCTTGACGGAGTGGGGGCTCAGCGTGGTCCGGTTGAGCCGCTTCTTATGCTTCTTGTCg                                                     >  1:4372450/1‑140 (MQ=17)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGTTCGTGTTGACg                                                     >  1:3534818/1‑140 (MQ=14)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTTACGGATTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTGTTGACg                                                     >  1:5175943/1‑140 (MQ=255)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                     >  2:1593395/1‑140 (MQ=255)
                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCGTCTTTACg                                                     >  1:3891810/1‑140 (MQ=255)
                                                                                    cGTGCTCCGGTTGCGCCGCTTGTTTCTCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  2:3765830/140‑1 (MQ=255)
                                                                                    cGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  1:2312879/140‑1 (MQ=255)
                                                                                    cGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  2:3789199/140‑1 (MQ=255)
                                                                                    cGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTGGAGCCGCTTCTTCTGCTTCTTGCCgg                                                    >  2:211201/1‑140 (MQ=255)
                                                                                     gTGGTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGTAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGa                                                   <  1:4194164/140‑1 (MQ=255)
                                                                                     gTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGa                                                   <  1:1399479/140‑1 (MQ=255)
                                                                                        gtCCGGGTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  2:3018685/139‑1 (MQ=255)
                                                                                        cTCCGGTTGCGCCGCTTCTTGCTCTGCTTGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTAGACGGAGTGCGGGATCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  2:2484920/140‑1 (MQ=17)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTGTTCTTGACGGAGTGCGGGCTCCACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCGTCTTGTGCTTGTTGACGGAATg                                                >  1:1769472/1‑140 (MQ=17)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGTTCTTCTTGACGGGATg                                                >  1:2812573/1‑140 (MQ=255)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                >  1:3331533/1‑140 (MQ=255)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  1:5141107/140‑1 (MQ=255)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  1:90735/140‑1 (MQ=255)
                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  1:2113724/140‑1 (MQ=255)
                                                                                        cTCCGGTTGAGCCGCTTCGTTCTCTTGTTGACGGTGTGCCGGCTCAACGTGCTCCGGTTGAGCCCCTTCTTCTGGTTCTTGACGGAGTGCGGGCGCAGGGTGCTCCGGTTGGTCCGCTTCGTCTGCGGGGTGACGGAATg                                                >  1:690442/1‑140 (MQ=17)
                                                                                                      cttcttTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGc                                  <  1:919957/140‑1 (MQ=255)
                                                                                                      cttcttTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGATTGCGGGCTCAGCGTGCTCCGGTTTAGCCGCTTCGTGTTCTTCTTTACGGAATTCAGGCTAAGCGTGc                                  >  1:1891405/1‑140 (MQ=14)
                                                                                                      cttcttTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCGTCTGCTTCTTGACGGAGTGCGGGCTCAGAGTGCTCCGGATGAGCAGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTgg                                  >  2:2330705/1‑139 (MQ=17)
                                                                                                      cttcttTCTCGTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTATGCTTGTTGACCGAAGGCAGGCTCAGCGTGc                                  >  2:1955435/1‑140 (MQ=17)
                                                                                                                ttcttGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCGGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAg                        <  2:1301533/140‑1 (MQ=255)
                                                                                                                 tcttGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTg                                                                                                                  <  2:527467/49‑1 (MQ=38)
                                                                                                                     gACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAgctgct                   <  1:2715018/140‑1 (MQ=255)
                                                                                                                     gACGGAGTGCCGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGTAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAgctgct                   <  2:1561368/140‑1 (MQ=255)
                                                                                                                        ggAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       >  1:2192984/1‑53 (MQ=35)
                                                                                                                        ggAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:2192984/53‑1 (MQ=35)
                                                                                                                          aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCGGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt              >  2:4389624/1‑140 (MQ=255)
                                                                                                                            tGCTGGCTCATAGTGCTCCGGTAGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct            <  2:2569003/140‑1 (MQ=255)
                                                                                                                            tGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct            <  2:2614704/140‑1 (MQ=255)
                                                                                                                            tGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct            >  1:3628041/1‑140 (MQ=255)
                                                                                                                            tGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct            <  2:3663272/140‑1 (MQ=255)
                                                                                                                                 gCTCAACGTGCTGCGGTTGAGCCGCTTCTTCGGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCtcttct       <  2:3008297/140‑1 (MQ=255)
                                                                                                                                    cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTATTCTTGa    >  2:288352/1‑140 (MQ=255)
                                                                                                                                    cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa    <  2:74719/140‑1 (MQ=255)
                                                                                                                                    cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCGCTTCTTGa    >  2:5118047/1‑140 (MQ=255)
                                                                                                                                     aaCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc   <  2:4815866/140‑1 (MQ=255)
                                                                                                                                     aaCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc   <  1:2667874/140‑1 (MQ=255)
                                                                                                                                      aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGTGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGGTCCGGTTGTGCTGCTTCTTTCTCTTCTTGACg  >  2:3862157/1‑140 (MQ=255)
                                                                                                                                      aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGTCGGAATGCCGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg  >  2:3609108/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
CTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACG  >  NZ_CP034943/2680266‑2680539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: