Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,812,782 T→G 7.1% K202T (AAG→ACG)  EO946_RS14520 ← SDR family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,812,7820TG 7.1% 508.1 / 3.0 249K202T (AAG→ACG) EO946_RS14520SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (107/107);  new base G (29/2);  total (140/109)
Fisher's exact test for biased strand distribution p-value = 1.31e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCC  >  NZ_CP034943/2812645‑2812921
                                                                                                                                         |                                                                                                                                           
   tGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTg                                                                                                                                        <  1:5110132/140‑1 (MQ=255)
   tGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTg                                                                                                                                        <  1:1865324/140‑1 (MQ=255)
   tGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGGTGTCCTCACACAAGACAGCGCTAATCCGGGCGATATCCTCCCCTGTGCCAGAACTTCCTAACGGCGTTTTTTCCTTGCCGATTCGCTTCCTg                                                                                                                                        <  1:2758044/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCACTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  2:1572524/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  2:4745963/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  2:3243398/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  1:775828/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  1:2077820/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      <  2:3698358/140‑1 (MQ=255)
        ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATCATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                   <  2:3354876/140‑1 (MQ=255)
          tCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCct                                                                                                                                 <  1:1100677/140‑1 (MQ=255)
          tCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCct                                                                                                                                 >  1:3861182/1‑140 (MQ=255)
          tCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCct                                                                                                                                 >  2:557954/1‑140 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTACTGGGTTCctc                                                                                                                                >  1:258601/1‑140 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCctc                                                                                                                                >  1:3694208/1‑140 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCGTTCCGATTCGCGTCCTGGGGTCctg                                                                                                                                >  1:3944360/1‑139 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                        <  2:2483085/140‑1 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                        <  2:3426533/140‑1 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                        <  2:4426898/140‑1 (MQ=255)
                    tCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCAATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGc                                                                                                                       <  2:4373468/140‑1 (MQ=255)
                      aaTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGctt                                                                                                                     >  2:4628567/1‑140 (MQ=255)
                      aaTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGATTCGCATACTGGCGTCCTCTATTGATGgtt                                                                                                                     >  2:1389425/1‑140 (MQ=255)
                             gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGCGTGCGCTAGTGATGGTTCCTTAAt                                                                                                              >  1:4017532/1‑140 (MQ=255)
                             gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCAt                                                                                                              >  2:2267608/1‑140 (MQ=255)
                             gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCAt                                                                                                              >  2:399866/1‑140 (MQ=255)
                               gCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATTCTCCGGGCGATATCCTCGGCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGt                                                                                                            <  2:3746730/140‑1 (MQ=255)
                               gCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGt                                                                                                            <  2:1190885/140‑1 (MQ=255)
                                ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGATTCGCTTACTGGCTTCCTCTATTGATGCTTCCTTCATGTc                                                                                                           >  2:2857900/1‑140 (MQ=255)
                                    tCACAAGGTCTGAGTTCTCCTCAGACACGTAAGCGATATTCGGGGCGATATCCTGGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCCTGTCGCCg                                                                                                       <  2:4902348/140‑1 (MQ=255)
                                    tCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCg                                                                                                       <  2:730822/140‑1 (MQ=255)
                                    tCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCg                                                                                                       <  2:4538690/140‑1 (MQ=255)
                                        aaGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACaa                                                                                                   >  2:2102098/1‑140 (MQ=255)
                                          ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCCCTTCGCTTCCTGGCGTCCGCGGCTGATGCGTCCTTCATGTCGCCGACaaag                                                                                                 >  2:900004/1‑138 (MQ=255)
                                          ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTTCTTGCCGATTCGCTTACTGGCGTCCGCTATTGTTGCTTCCGTCATGTAGCCGACaaag                                                                                                 >  1:2408019/1‑138 (MQ=255)
                                           gTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGt                                                                                                <  2:2276051/140‑1 (MQ=255)
                                              tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAc                                                                                             >  2:3542945/1‑140 (MQ=255)
                                              tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGGGTGCTCTCTTGATGGTTGCTTCATTTCGCCGACAATGTCAc                                                                                             >  1:296898/1‑140 (MQ=255)
                                                  ttCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCgg                                                                                         <  1:4726347/140‑1 (MQ=255)
                                                  ttCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCgg                                                                                         <  1:2774200/140‑1 (MQ=255)
                                                  ttCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCgg                                                                                         <  2:2561893/140‑1 (MQ=255)
                                                    ctcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                  <  2:3881388/129‑1 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCAGGCTTCCTCGAGTGATTCGTACTGAAAGTCGCCGACAATGTGACCCCGGcc                                                                                      >  1:4433308/1‑139 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCCATTCGCTTGCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCCCCCGGGcc                                                                                      >  2:1748880/1‑139 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTTCCGATTCGCGTCCTGGCTTCCTCTAATGATGCTTCCATCATTTCGCCGCCAAAGTCACCCCGGCa                                                                                      >  1:1694435/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                      >  1:3189692/1‑91 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCGTCCTTCATTTCGCCGACAAAGTCACCCGGGcca                                                                                     >  1:1214717/1‑138 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCGCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGcca                                                                                     >  2:624334/1‑138 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCATGCCGCTTGGGATGCAGGCTGACGCGATTGATGGTTACTTAAATTAGGCGACAAAGTAACCCGGGcca                                                                                     >  1:1787089/1‑138 (MQ=255)
                                                        tcAGACAATAAAGATATTATACGGGCGATATCCTCGACTGTGCCAGATCTTCCTATCGGCGTTTTGTCCTTGCCTTTTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAc                                                                                   <  1:5031665/140‑1 (MQ=255)
                                                        tcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAc                                                                                   <  2:2354735/140‑1 (MQ=255)
                                                            acaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGCTTCGCTTCCTGGCTTCCTCTATTGATGCGTCCTTCATTTAGCCGACAATGTCACCCGGGCCAACCCTg                                                                               >  2:3869059/1‑140 (MQ=255)
                                                               aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCGTGCTGGCTTGCTCGATTTATGCGTGCGTGATGTGGCCGACCATGTCACCCGGGCCAACCagggtg                                                                            >  1:2242420/1‑135 (MQ=255)
                                                               aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTg                                                                            >  2:4210795/1‑140 (MQ=255)
                                                                gtaaGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGATTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTgg                                                                           <  2:458669/138‑1 (MQ=255)
                                                                 aaaGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTCGCGTCCTGGCGTCCTCTATTGATTCTTCCGTCATGTCGCCGACAAAGTCACCCGGGCAAACCAAGTTTGc                                                                          >  1:2422148/1‑140 (MQ=255)
                                                                   aGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTGGCGTCCTGGGGTGCTCTATTGATGCTTCCGTCATGTCGCCGACAAAGTCACCCGGGCAAACCAGGTTGGccg                                                                        >  2:792070/1‑138 (MQ=255)
                                                                   aGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGGTTCCTTCATTTCGCCGACAATGTCAACCGGGCAAAACATGTTGGCAg                                                                        >  2:5031665/1‑140 (MQ=255)
                                                                    gCGATAATCCGGGCGATAGCCTAGCCTGTGACAGATCTTACTATCGGCGTTTTTTCCTAGCCCATTAGCGTCCTGGCTTCCTCTGATGTTGCTTCCATCAATTAGCAGACCATGTCAACCGGGAAAACCATGtagtcatt                                                                       >  2:2472977/1‑133 (MQ=255)
                                                                      gATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTa                                                                     >  2:928392/1‑140 (MQ=255)
                                                                        tAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCGTCCTGGCGTGCGCTATTGATGGTTCCTTCAAGTCGCCGCCAATGTGACCCCGGCCAACCCTGTTGGCAGttttt                                                                   >  1:1964841/1‑137 (MQ=255)
                                                                        tAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATg                                                                   <  1:793179/140‑1 (MQ=255)
                                                                          ctCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGcc                                                                 <  2:3986754/139‑1 (MQ=255)
                                                                          aTCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGcc                                                                 <  2:3138996/140‑1 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTGGCGTCCTGGGGTGCTGTATTGATTCTTGCGTCATGTCGCCGACAAAGTCACCCGGGCCAAACAGGTTGGCAGTTATGCCgcaat                                                            >  1:1421464/1‑136 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGCGTACGCTATTGATGCTTCCTTCAAGTCGCCCACAAAGTCACCCGGGCACACCAAGTTGGCAGTTATGCCGAAtt                                                            >  1:4990777/1‑140 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCTTCCTGGCGTGCTCTATTGATGCTTCCATCATGTCGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAAtt                                                            >  2:3148929/1‑140 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGATTCGCTTCCTGGGTTCCTCTATTGATGGGTCCTTCATGTCGCCGACAAAGTCACCCGGGCAAACCATGTTTGGCGGTATGCCGAAtt                                                            >  1:3426547/1‑140 (MQ=255)
                                                                                   atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                        <  2:2022324/140‑1 (MQ=255)
                                                                                   atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                        <  2:1473511/140‑1 (MQ=255)
                                                                                   atatCCACGCCGGGGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGCTTCGCGTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                        <  2:1173268/140‑1 (MQ=255)
                                                                                          cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTca                                                 <  1:918309/140‑1 (MQ=255)
                                                                                          cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACGTGCCGATTCGGGTCCTGGCGTCCGCTAGTTATGCTTCCTTCATGTGGCCGACCAAGTGACCCCGGCAAACCAGGTTTGGGGGTAATCCGAAAAGGGCTTCTTca                                                 >  1:4518202/1‑140 (MQ=255)
                                                                                          cGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGAGTCGCGTACTGGCTTGGTCGAATGTTGCTTCCTTCATGTCGCCGCCAATGTCCCCCGGGCCCACCCTGTTGGCAGTTATGCCGAATTCGGCGTGTTca                                                 >  1:4649117/1‑140 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTGCTTGCGTGCTCTATTGATTCTTGCTTAATGTCCCCGACAAAGTCACCCGGGCCAACCATGTTGGGCGTTATTCCGAATTCGGCTTCTTaaatggca                                           >  2:231843/1‑140 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTGTTTTACTTTCCGATTCGCTTCCTGGCGTGCTCTATTGTTGGTTCCTTCATGTCGCCGACAAAGTCACCCCGGCCAACCAGGTTGGCAGTTATTCCGAAATGGGCTTCTTcaaatgca                                           >  1:2554898/1‑135 (MQ=255)
                                                                                                 gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                          <  1:1240319/140‑1 (MQ=255)
                                                                                                 gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                          <  2:3624810/140‑1 (MQ=255)
                                                                                                     gATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGc                                                                                                                       <  1:1552810/59‑1 (MQ=255)
                                                                                                     gATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGt                                      >  2:3799118/1‑140 (MQ=255)
                                                                                                      aTCTTCCTATCGGCGTTTTTTGCTTTCCGATTGGCTTGCTGGCTTCCTCTATTGATTCTTGCTTGATGTCGCCGGCAATGTCACCCGGGCAAACCATGTTTGCAGTTATTACGAATTCGGCTTCTTAAATGGCAATTGtg                                     >  2:502458/1‑139 (MQ=255)
                                                                                                          tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATTTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCg                                 >  2:4446830/1‑140 (MQ=255)
                                                                                                          tCCTATCGGCGTTTTTTCCTTGCCGATTCGCATCCAGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCAAGTTGGCAGTTATTCCGAAATCCGCTTGTTCAATTGCAATGGTCttag                                 >  2:5140836/1‑138 (MQ=255)
                                                                                                          tCCTATCGGCGTGTTTTGCTTTCCGCTTCGCTTCCTGGCTTCCGCAATTGATGCTTCCGTCATGTCGCCGACAATGTCACCCGGGCCAACCATGTTGGCAGTTATGCCGAAATCGGCTTCTTCAATGGCAATTGTGttag                                 >  1:2295048/1‑138 (MQ=255)
                                                                                                                cGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAaag                           <  1:4163563/140‑1 (MQ=255)
                                                                                                                     ttttttCCTTGCCGATTCGCTTCCTGGCTTCCGCTATTGATTCTTCCTTCATGTCGCCCACAATGTCACCCCGGCAAACCAAGTTGGCAGGTATTCCGAATTAGGCTTAGGCAATGGCAAATGTATTCCTCAAACAAGcc                      >  2:1365002/1‑140 (MQ=255)
                                                                                                                     ttttttCCTTGCCGATTAGCTTCCGGGCTTCCTCTATTGATGCTTCCGTCATGTGGCCCACAAAGTCACCCGGGCAAACCATGTTTGCAGTTATGCCGAATTCGGCTTCTTAAAAGGCAATGGTCTTCGGCAAAGTAGcc                      >  2:1654263/1‑140 (MQ=255)
                                                                                                                     tgttttCCTTTCCGAGTCGCTTGCTGGCTTCCTCTATTGATGCTTCCTTCAATTCGCCGACCATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCCAATTCGGCTTAATCAATGGCAATTGTCTTCGTCAAAGAATcc                      >  2:5096492/3‑140 (MQ=255)
                                                                                                                        tttCCTTGCCGATTCGCTTCCTGGCTTCCTCTCTTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCaaa                   <  1:737244/140‑1 (MQ=255)
                                                                                                                        tttCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCaaa                   <  1:3046783/140‑1 (MQ=255)
                                                                                                                        tttCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCaaa                   <  1:3802329/140‑1 (MQ=255)
                                                                                                                        tttCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCaaa                   <  1:5140836/140‑1 (MQ=255)
                                                                                                                             ttGCCGATTCGCTTCCTGGCTTCCGCTATTTATTCTTACTTCAAGTCGCCGACAAAGTCACCCCGGCAAACCATGTTTGCAGTTATTCCCAATTCGGCTTATTCAAATGCCATTGTCTTAGTCAAAGAACCCAAAaccca              >  1:3120779/1‑138 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  1:358516/140‑1 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  1:962000/140‑1 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       >  2:262081/1‑140 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  1:1655842/140‑1 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  1:1311736/140‑1 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  1:633298/140‑1 (MQ=255)
                                                                                                                                    ttCGCTTCCGGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  2:4112408/140‑1 (MQ=255)
                                                                                                                                    ttCGCGTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa       <  2:2945890/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCC  >  NZ_CP034943/2812645‑2812921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: