Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,982,236 T→C 24.2% noncoding (284/1550 nt) EO946_RS15435 ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,982,2360TC24.2% ‑3.9 / 7.3 24noncoding (284/1550 nt)EO946_RS1543516S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (3/14);  new base C (2/3);  total (7/17)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATCCGGTATTAGCCCCGGTTTCCCGGA  >  NZ_CP034943/2982100‑2982369
                                                                                                                                        |                                                                                                                                     
tcttcactcacGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTCCTGCTGCCTCACGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGcc                                                                                                                                    <  2:5165830/137‑1 (MQ=0)
     acgcacGCGGCGTTGCTCCGTCAGACCTTCGTCCATTGCGGAAGATTCCCGACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTGGGt                                                                                                                               <  1:1854002/137‑1 (MQ=0)
     acgcacGCGGCGGTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGt                                                                                                                               <  2:2736165/137‑1 (MQ=1)
               cggtGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTAc                                                                                                                     <  2:4198873/137‑1 (MQ=1)
                    ctgcGTCAGAATTTCGGCCATTGCGGAAGAGTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTGGGTGAGCCATTACCTCAc                                                                                                                <  1:4316902/137‑1 (MQ=1)
                                       atggCGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGGCCCAGTGTGGCCGATCACCGTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCAGCAACTAGCTAATGcgccgc                                                                                             <  2:2084947/137‑1 (MQ=1)
                                              aataTTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCa                                                                                      <  1:2756433/137‑1 (MQ=1)
                                              aataTTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCa                                                                                      <  1:2757090/137‑1 (MQ=1)
                                              aataTTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCa                                                                                      <  2:2273677/137‑1 (MQ=1)
                                              aataTTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCa                                                                                      <  2:2014965/137‑1 (MQ=1)
                                              aacaTTCCCTACTGCTGCATCACGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCa                                                                                      >  2:30544/4‑140 (MQ=0)
                                                          tgatgcCTCCCGTAGGAGTCTGGGCCGTGTCTCAGACCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCGTCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGt                                                                          >  2:776715/4‑140 (MQ=0)
                                                            catactaccGTAGGAGTCTGGGCCGTTTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAg                                                                        >  1:1019686/8‑140 (MQ=0)
                                                               ccccccGTAGGAGTCTGGGCCGGGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCg                                                                     <  2:4625615/137‑1 (MQ=1)
                                                                   ccttAGGAGTCTGGGCCGTGTATCCGTCCCAGTGTGGCCGATCCCCCTCGCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAAATAGCTAATGCGCGGCGGGTCCTTGTTTAAGTGGTAGCCGAAGc                                                                 <  2:2751808/137‑1 (MQ=1)
                                                                          tgtgtGGGCCTTGTCTCAGTCCCAGTGTGTCCGATGACCCGGTCTGCTAGGCTTCGCTTCGTTGCCTTGTTTAGCGATTACCTCACCAACTAGCTAATTCGCCGCGTGTCCATCTTTAAGTGGTAGCCGAAGCCAGCttt                                                          <  2:1202215/136‑1 (MQ=0)
                                                                          aggcTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGGTGCCTTGGTGAGCCATGACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCttt                                                          <  2:3084405/137‑1 (MQ=0)
                                                                             ctcggCCGTGTCTCAGTCCCAGTGTGCCCGATCACCCTCTCAGGTCGGCTACGCATCGTAGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACAtattat                                                       >  2:3729183/4‑140 (MQ=1)
                                                                              tgtgCCGTCTCTCAGTACCATTGTGGCCGATCACCCTATCAGGTCGGCTACGCATCGTAGCCTTGGTGAGCCATAACCTAAACAACTAGCTAATGCTCCCCGTGTCCATCTGTAATTGGTAGCCGAAGCCACCTTTTATg                                                      >  2:1664007/4‑140 (MQ=1)
                                                                                     tggctcAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAAc                                               >  1:1374308/4‑140 (MQ=1)
                                                                                         tccgTCCCAGTGGGGCCGATCACCCACTCAGGTCGGCTTCGCATCGTTGCCTAGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGCACCATg                                           <  2:3711196/137‑1 (MQ=1)
                                                                                                   tggggCCGATCACCCTCTCAGGTCGGCTACGCCTCGTCGCCTTGGTGAGCCAGTACCTCACAAACTAGCTATTGCGCCGCGGGTCACTCTGTAAGTGGGAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTAAAAc                                 <  2:4288031/137‑1 (MQ=1)
                                                                                                          gagcACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGGGGTAGCCGAAGCCACCTTTTATGTTTGACCCCTGCGGTTCAAACAAGCATc                          <  1:3171938/137‑1 (MQ=0)
                                                                                                                                  caacGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCAACTGTAAGTGTTATCCGAAGCCACCTTTTATTTTTTAACCAAGCGGTTCAAACAAGCATCCCGTATTATCCCCCGTTTACCGGa  >  1:4978932/4‑140 (MQ=1)
                                                                                                                                        |                                                                                                                                     
TCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATCCGGTATTAGCCCCGGTTTCCCGGA  >  NZ_CP034943/2982100‑2982369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: