Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 361,344 T→G 6.8% V144V (GTT→GTG EO946_RS01960 → FAD/NAD(P)‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943361,3440TG 6.8% 1053.1 / 10.6 451V144V (GTT→GTGEO946_RS01960FAD/NAD(P)‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (227/170);  new base G (51/2);  total (279/172)
Fisher's exact test for biased strand distribution p-value = 1.58e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

AGCACTGCGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGG  >  NZ_CP034943/361205‑361482
                                                                                                                                           |                                                                                                                                          
  cACTGCGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGtttt                                                                                                                                          >  1:4618310/1‑140 (MQ=255)
      gCGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACATGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTTTTGGAAGCGGAGACAGATAGCTCTTTTAATCTCAGGGGGGAAGGAGTAGTTTTatga                                                                                                                                      <  1:1367946/140‑4 (MQ=255)
       cGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGttttttcca                                                                                                                                     >  2:140236/1‑135 (MQ=255)
       cGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCa                                                                                                                                     >  1:3643865/1‑140 (MQ=255)
       cGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCa                                                                                                                                     >  2:2409003/1‑140 (MQ=255)
       cGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCa                                                                                                                                     >  2:3612303/1‑140 (MQ=255)
        gagaCTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGACGGGCAAACGGGTTGTGGGAAGCGGAGCCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAt                                                                                                                                    <  2:3870055/140‑1 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGACGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTATCCAAGAGtatt                                                                                                                             >  1:1992043/1‑140 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTATCCATTAGtttt                                                                                                                             >  1:3438945/1‑140 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGtt                                                                                                                                            >  1:2279476/1‑125 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGtttt                                                                                                                             >  1:4141713/1‑140 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGtttt                                                                                                                             >  1:5169120/1‑140 (MQ=255)
               tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCAAGAGtttt                                                                                                                             >  1:4958669/1‑140 (MQ=255)
                 cTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTtc                                                                                                                           >  1:3064694/1‑140 (MQ=255)
                     aTGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcat                                                                                                                       <  2:458924/140‑1 (MQ=255)
                     aTGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcat                                                                                                                       <  2:3940249/140‑1 (MQ=255)
                     aTGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcat                                                                                                                       <  2:114565/140‑1 (MQ=255)
                        aaaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAg                                                                                                                    <  2:4959060/140‑1 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCACACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCATGAGTTTTTCTCATAAGc                                                                                                                   >  2:2647345/1‑140 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGAGTTTTTCTCATCAGc                                                                                                                   >  1:1881190/1‑140 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCCTGAGTTTTTCTAAACAGc                                                                                                                   >  2:2817092/1‑140 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcatcatc                                                                                                                   >  2:1357554/1‑138 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTATCCATGAGTTTTTCTAATCAGc                                                                                                                   >  2:2800912/1‑140 (MQ=255)
                         aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGc                                                                                                                   >  2:3736317/1‑140 (MQ=255)
                              atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGttt                                                                                                                                           <  1:3253063/111‑1 (MQ=255)
                              atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGttt                                                                                                                                           >  2:3253063/1‑111 (MQ=255)
                              atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCcttct                                                                                                              <  1:3848459/140‑1 (MQ=255)
                              atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATAAGCcttct                                                                                                              >  2:1902182/1‑140 (MQ=255)
                               tatGGATCAAGCATGGAAAACAGGCATGGTGCCAAATTTGAGAAGGGCAAACGGGGTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCCGCcttctt                                                                                                             >  2:23138/1‑140 (MQ=255)
                               tatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTACCCATGAGTTTTTCTCCTCCGccgtagt                                                                                                             >  2:2367610/1‑135 (MQ=255)
                                   gATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCATGAGTTTTTATCATAAGCCGTCTTGGcc                                                                                                         >  1:3787235/1‑140 (MQ=255)
                                    aTCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCg                                                                                                        >  2:290894/1‑140 (MQ=255)
                                     tCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGATTTTTTCCCATCAGCCTTCTTGGCCgg                                                                                                       >  2:1289942/1‑140 (MQ=255)
                                        aGCATGGAAAACAGGCATGGTGCCAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTCTCATCACCCTTCTTGGGCGGAGt                                                                                                    >  1:1618893/1‑140 (MQ=255)
                                        aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTCTCATAAGCCTTCTTGGGCGGAGt                                                                                                    >  1:4432771/1‑140 (MQ=255)
                                        aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGcc                                                                                                                  >  1:2358090/1‑126 (MQ=255)
                                        aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTTGCCGGAGt                                                                                                    >  2:4144758/1‑140 (MQ=255)
                                        aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGt                                                                                                    >  2:3050537/1‑140 (MQ=255)
                                            tGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCCGGAGTTTTTGTCAGCACCCGTCTTGGCCGGAGTGGGc                                                                                                >  1:791266/1‑140 (MQ=255)
                                              gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCCTGAGTTTTTCTCATCACCCTTCTTTGCCCGACTGGGcca                                                                                              >  2:1016864/1‑138 (MQ=255)
                                              gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCaa                                                                                              >  1:2189103/1‑140 (MQ=255)
                                                 aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGCAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                           <  1:3299154/140‑1 (MQ=255)
                                                 aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                           <  1:5071328/140‑1 (MQ=255)
                                                    aGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCCGAGTGGGCAAAACCAt                                                                                        >  2:807549/1‑140 (MQ=255)
                                                      gCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCCAGAGTTTTTATCATCAGCCGTCTTGGCCGGAGTGGGCAAAAcaaatt                                                                                      >  1:399072/1‑135 (MQ=255)
                                                         tGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTATCCATGAGTTTTTGTAATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAg                                                                                   >  1:1045941/1‑140 (MQ=255)
                                                              aCAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAg                                                                              <  1:3231753/140‑1 (MQ=255)
                                                                aaaTTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCATCAGCCGTCTTGGCCGGAGTGGGCAAAACCATTTAAGAATAGCg                                                                            >  1:1512977/1‑140 (MQ=255)
                                                                 aaTTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCgg                                                                           <  2:5136326/140‑1 (MQ=255)
                                                                   tttGAGCAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCa                                                                         <  2:4443868/140‑1 (MQ=255)
                                                                           gggCAAACGGGTTGTGGGAAGCGTAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAt                                                                 <  2:3338889/140‑1 (MQ=255)
                                                                           gggCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCATGAGTTTTTCTCAACCGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATCGCGGCATTTATCAt                                                                 <  2:1565717/140‑1 (MQ=255)
                                                                              cAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAACAATTCAGTATAGCGGCATTTATCAtgtg                                                              >  2:3387588/1‑140 (MQ=255)
                                                                              cAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTACCCAATATTTTTTATAATCAGCCGTCTTTGCCGGAGTGGGCAAAACCATTTAAGAAAATCGGCATTTAtaaagtt                                                              >  2:4006056/1‑134 (MQ=255)
                                                                                aaCGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGtt                                                            <  1:1447783/140‑1 (MQ=255)
                                                                                   gggTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGa                                                         <  2:1756782/140‑1 (MQ=255)
                                                                                     gTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGGGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                       <  1:4710486/140‑1 (MQ=255)
                                                                                     gTTGTCGGAAGCCGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                       <  2:78918/140‑1 (MQ=255)
                                                                                      ttGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGGTTTTGTCATAAGCCTTATTTGGCGGAGTGGGCAAAAACATTTAAGTATAGCGGCATTTATCATTTGTTTGagag                                                      >  2:4358548/1‑140 (MQ=255)
                                                                                      ttGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGGCGGAGTGGGCACAAACATTTCAGAATAGCGGCATTTATAATGTGttttaaag                                                      >  1:5031824/1‑135 (MQ=255)
                                                                                        gtgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACagag                                                    <  1:1237224/140‑1 (MQ=255)
                                                                                         tgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAtgtg                                                              >  2:2985855/1‑129 (MQ=255)
                                                                                         tgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGCGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACagaga                                                   <  2:3801014/140‑1 (MQ=255)
                                                                                             aaGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTg                                               <  2:4523506/140‑1 (MQ=255)
                                                                                              aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                              <  2:4749908/140‑1 (MQ=255)
                                                                                              aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                              <  2:1089227/140‑1 (MQ=255)
                                                                                              aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                              <  2:2016829/140‑1 (MQ=255)
                                                                                              aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                              <  1:2853135/140‑1 (MQ=255)
                                                                                               gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAATAGTTTTTGTCATAAGCCTTCATGGGCGGAGTGGGCAAAAACAATTCAGAAAATCGGCATTTATAATGTTTTTTTGAGAGAGGTGcc                                             >  1:714819/1‑139 (MQ=255)
                                                                                               gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCa                                             <  2:2189103/140‑1 (MQ=255)
                                                                                                     aCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGTGTTTTTCTCATAAGCCTTCTTGGGCGGGGTGGGCCCAACCAATTAAGAAAAGCCGGATTTATCATTTGTTTTGCCGCGAGGTGCACGAGGt                                       >  1:1077929/1‑140 (MQ=255)
                                                                                                      cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCGGGAGGGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGAACTAGAGGTGCACGATGTa                                      >  2:3107292/1‑140 (MQ=255)
                                                                                                      cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                      <  1:3387148/140‑1 (MQ=255)
                                                                                                      cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                      <  1:2080776/140‑1 (MQ=255)
                                                                                                      cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                      <  1:274777/140‑1 (MQ=255)
                                                                                                      cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                      <  1:379721/140‑1 (MQ=255)
                                                                                                        gagaCCGCTGGTACTCTCAGGGGGGAAGGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATACCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAg                                    <  1:2978857/137‑1 (MQ=255)
                                                                                                          tACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATCAGCCTTCTTTGCCGGAGTGGGCAAAACCATTTGAGAATAGCCGCATTTATAATGTGGTTGAAAGAGAGGTGCACGATGTAAGCa                                  >  2:1856361/1‑140 (MQ=255)
                                                                                                          tACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTATCCATGAGTTTTTGTCATAAGCCGTCTTGGCCGGAGTGGGCCAAACCAATTCAGAATAGCGGCATTTATCATGTGTTTTTCAGAGAGGTGCACGATGTAAACa                                  >  1:4335538/1‑140 (MQ=255)
                                                                                                                 ggTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGGCGTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATGATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAg                           >  2:1226228/1‑140 (MQ=255)
                                                                                                                 ggTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAg                           <  1:3508284/140‑1 (MQ=255)
                                                                                                                 ggTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGACTTTTTATGATACGCCTTGTTTGCCGGAGTGGGCCAAACCATTTGAGAAAAGCGGCATTTATCATTTTTTTGACAGAGAGGTGGACGATGTAAGCAATGAAAg                           >  2:553826/1‑140 (MQ=255)
                                                                                                                   tACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcat                                                                                                                       <  1:2397724/46‑1 (MQ=255)
                                                                                                                   tACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTATCATCAACCGTCTTGGCCGGAGTGGGCAAAACCATTTCAGAAAAGCGGCAATTATCATGTGTTTTACAGAGAGGTGCACCATGTAAGCAATGAAAGTc                         >  2:2526449/1‑140 (MQ=255)
                                                                                                                       ctcAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCa                     <  1:4225363/140‑1 (MQ=255)
                                                                                                                       ctcAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCa                     <  1:140236/140‑1 (MQ=255)
                                                                                                                         cAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCaaa                   <  1:1586776/140‑1 (MQ=255)
                                                                                                                          aGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTATCATAACCCTTCTTGGGCGGAGTGGGCAAAAACAATTAAGAATAGCGGCCTTTATAATGTGTTTGACAGAGAGGTGCACGATGTAAACAAATAAAGTCTTCaaaa                  >  1:4810667/1‑139 (MQ=255)
                                                                                                                          aGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCGTTGCCCGATTGGGCAAAACCATTTCAGAATAGCGGGCTTTATCATGTTTTTGAACGAGAGGGTCCAGATGTAAGCAAAGTAAGTCTGCaact                  >  2:3520374/1‑138 (MQ=255)
                                                                                                                            gggggAACGAGGCGTTTTAGCCATGAGTTTTGCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCCGAATAGCGGCATTTCTCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATgg                <  1:1728428/140‑1 (MQ=255)
                                                                                                                               ggAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTAAGAATAGCCGCATTTAACATGTGTTTGACCGAGAGGTTCACCATGTAAGCAAATAAAGTCTTCAAAAGGCAg             >  2:4287027/1‑140 (MQ=255)
                                                                                                                                 aaCGAGTCGTTTTAGCCCTGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAACATTTCAGAATAGCGGCATTTATAATGTGTTTGAGAGAGAGGTGCACGATGTAAGCAATGAAAATCGTCCAATGGCACtt           >  2:4908459/1‑140 (MQ=255)
                                                                                                                                  aCGAGTCGTTTTAGCCCTGAGTTTTTCTCAACAGCCTTCTTTGGCGGAGTGGGCAAAACCATATAAGAAAAGCGGCAATTATAAAGTGTTTGACAGAGAGGTGCACGATGTAAGCAAGGAAACTATGGAAATTGCAGTtg          >  2:631179/1‑140 (MQ=255)
                                                                                                                                       tCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGACTAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGc     <  1:2580030/140‑1 (MQ=255)
                                                                                                                                       tCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGc     <  1:1942417/140‑1 (MQ=255)
                                                                                                                                       tCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGc     <  2:1595024/140‑1 (MQ=255)
                                                                                                                                       tCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGc     <  1:4543224/140‑1 (MQ=255)
                                                                                                                                        cGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCg    <  2:5041809/140‑1 (MQ=255)
                                                                                                                                         gTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCgg   <  2:1609222/140‑1 (MQ=255)
                                                                                                                                          ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg  <  1:864046/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGCACTGCGAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGG  >  NZ_CP034943/361205‑361482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: