Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,111,432 C→A 5.1% intergenic (‑333/+219) EO946_RS16070 ← / ← EO946_RS16080 GNAT family N‑acetyltransferase/iron‑sulfur cluster assembly accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,111,4320CA 5.1% 703.7 / 8.1 262intergenic (‑333/+219)EO946_RS16070/EO946_RS16080GNAT family N‑acetyltransferase/iron‑sulfur cluster assembly accessory protein
Reads supporting (aligned to +/- strand):  ref base C (164/74);  new base A (4/17);  total (171/91)
Fisher's exact test for biased strand distribution p-value = 1.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

GGTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGAAAAGCGTCAT  >  NZ_CP034943/3111297‑3111570
                                                                                                                                        |                                                                                                                                          
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAata                                                                                                                                        >  1:1068211/1‑140 (MQ=255)
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAata                                                                                                                                        >  2:4651335/1‑140 (MQ=255)
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAata                                                                                                                                        >  1:1883397/1‑140 (MQ=255)
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAata                                                                                                                                        >  1:2846206/1‑140 (MQ=255)
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTAAAata                                                                                                                                        >  1:1056904/1‑140 (MQ=255)
ggTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCGCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAata                                                                                                                                        >  2:1604052/1‑140 (MQ=255)
  tACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAAtata                                                                                                                                      >  2:2028692/1‑140 (MQ=255)
  tACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTATTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCACCTTTATTGCCTTTACAAtata                                                                                                                                      >  2:5083695/1‑140 (MQ=255)
     gAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAat                                                                                                                                   <  2:1528124/140‑1 (MQ=255)
     gAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAat                                                                                                                                   <  1:5055084/140‑1 (MQ=255)
      aaTATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatg                                                                                                                                  >  2:2923273/1‑140 (MQ=255)
          ttGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaata                                                                                                                              >  2:915065/1‑138 (MQ=255)
          ttGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaata                                                                                                                              >  2:2329800/1‑138 (MQ=255)
          ttGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaaga                                                                                                                              >  2:2892906/1‑140 (MQ=255)
          ttGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGAATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCAAACTTTATTTCATTTAGAATATAAatgaagt                                                                                                                              >  1:3844839/1‑139 (MQ=255)
          ttGCCCTTGCTGCTACTCCAATTTCATGAAGAAGCTTCCTCATCATCAACTCGGCAATTGTATTTGTTAGTGCAAATGCAATCAAAATACCGTACGGTCAAGATAACCCCACTTACTTGCAGTTACAATATAAaggtaga                                                                                                                              >  2:3017677/1‑140 (MQ=255)
           tGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagat                                                                                                                             >  1:2562161/1‑140 (MQ=255)
           tGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagat                                                                                                                             >  2:4251966/1‑140 (MQ=255)
           tGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagat                                                                                                                             >  2:2641503/1‑140 (MQ=255)
           tGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGAATTTACAATATAAatgaaggt                                                                                                                             >  2:673081/1‑138 (MQ=255)
            gCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagatg                                                                                                                            >  2:4494750/1‑140 (MQ=255)
            gCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagatg                                                                                                                            >  2:3407837/1‑140 (MQ=255)
               cTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagatgaag                                                                                                                         >  2:2592469/1‑140 (MQ=255)
               cTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagatgaag                                                                                                                         >  2:1443058/1‑140 (MQ=255)
               cTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAatgaagatgaag                                                                                                                         >  2:1336851/1‑140 (MQ=255)
                  gctgctACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTTCAATATAATTGAAGATGAaggag                                                                                                                      >  1:3750842/1‑140 (MQ=255)
                  gctgctACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAaggag                                                                                                                      >  2:3461493/1‑140 (MQ=255)
                  gctgctACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGAAATAAAAATACCGTACGTTAAATATAACCAAACTTTATTGCATTTACAATATAAATGAAGATGAacgag                                                                                                                      >  1:4265877/1‑140 (MQ=255)
                       tACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTcgc                                                                                                                 <  1:3315858/140‑1 (MQ=255)
                       tACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTcgc                                                                                                                 >  2:1675677/1‑140 (MQ=255)
                           ccAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTaa                                                                                                             <  1:2266768/140‑1 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTGCTCAAATGTATTTTTTATTGCAAATTCAATAAAAATACCGTACGTTAAATATAACACAACTTTATTGCATTTACAATATAAATGAAGAGTAAGTAGGTGGCGTAAAGt                                                                                                          >  2:3246776/1‑140 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGt                                                                                                          >  2:4921671/1‑140 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGt                                                                                                          <  1:1166788/140‑1 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGt                                                                                                          >  2:3305271/1‑140 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGt                                                                                                          >  1:4115707/1‑140 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGt                                                                                                          >  2:480599/1‑140 (MQ=255)
                              aTTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGATAAATATAACCCAACTTTATTGAATTAACAATATAAATGAATATGAAGGAGTTCGCGTAAAGt                                                                                                          >  2:4481966/1‑140 (MQ=255)
                                   aTGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAAtt                                                                                                     >  2:4986830/1‑140 (MQ=255)
                                   aTGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAAtt                                                                                                     >  2:4271747/1‑140 (MQ=255)
                                     gaagaaTCTTCCTCATCATCAACTCCACAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTGAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAAtttt                                                                                                   <  2:3050427/140‑1 (MQ=255)
                                             ttCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTg                                                                                           >  2:297109/1‑140 (MQ=255)
                                             ttCCTCATCATAAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTTTCTTTTg                                                                                           >  2:4380647/1‑140 (MQ=255)
                                               ccTCATCATCAACTCCTCAAAGGTATTTTTTAGTGCAAATGCAATAAAAAGACCGTACGGTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGaa                                                                                         <  1:1293666/140‑1 (MQ=255)
                                                          aCTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAAt                                                                              >  1:4810689/1‑140 (MQ=255)
                                                          aCTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAAt                                                                              >  2:273485/1‑140 (MQ=255)
                                                              ctcAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTc                                                                          <  2:3499387/140‑1 (MQ=255)
                                                              ctcAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAAGTTTGTCTTTTGAAGCAATCTAAATTTTc                                                                          >  2:2426308/1‑140 (MQ=255)
                                                                   aTGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTccgccg                                                                     <  2:5108146/140‑1 (MQ=255)
                                                                        ttttttATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTACAGTGACTTTTGTCTTTTGAAGCAATCTCAATTTTCCGCCGTTTCt                                                                >  2:3071758/1‑140 (MQ=255)
                                                                        ttttttATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCt                                                                >  2:4414313/1‑140 (MQ=255)
                                                                        ttttttATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCt                                                                <  2:364930/140‑1 (MQ=255)
                                                                           tttATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCttta                                                             >  1:4108736/1‑140 (MQ=255)
                                                                             tATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCtttatt                                                           >  1:95252/1‑140 (MQ=255)
                                                                             tATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCtttatt                                                           >  1:2676025/1‑140 (MQ=255)
                                                                              aTTGCAAATGCAATAAAAATACCGTACGTTAACTATAACCCCACTTTATTGCATTTAGAATATACATGAAGATGAAGGAGGTCGCGTAAAGTGACTTTTGTGTGGTGAAGCTATCTAAATTTGCCGCCGTTTCtgtatgt                                                          >  1:883871/1‑138 (MQ=255)
                                                                                tGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCAGCCGTTTCTTTATTTaa                                                        >  1:739531/1‑140 (MQ=255)
                                                                                 gCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAg                                                       <  2:4108736/140‑1 (MQ=255)
                                                                                 gCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAg                                                       <  2:3621500/140‑1 (MQ=255)
                                                                                   aaaTGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGcc                                                     <  2:1121990/140‑1 (MQ=255)
                                                                                                atactttACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAATATGACGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGAAATCAAAATTTTCCGCCTTTTCTTAATTTAAGCAATAATAAAAACGc                                        <  1:1208681/134‑1 (MQ=255)
                                                                                                aTACCTAACTTTAAATAAAACCCAAATTTATTGAATTAACAATATAATTTAAGATGATGGAGGTCGCGAAAATTGAATTTTGTCTTTTGAACCAATCTAAATTTTCCGCCTTTTATTTTTTTAAGCAATAATAAAAACGc                                        <  1:4028595/140‑1 (MQ=255)
                                                                                                 taacGTACTTTAAATATAACCCAACTTTATTGCATTTACAATATAAATTAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGcc                                       <  2:4169195/137‑1 (MQ=255)
                                                                                                 tACCGTCCTGTAATTATAACCCAACTTTTTTGCATTTACAATATAATTTAAGATGAAGGTGGTCGCGTAAAGTGATTTTTGTGTTTTGAAGCAATCTAAATTTTCCGCGGTTTTTTTATTTAAGCCATAATAAAAACGcc                                       <  2:804755/140‑1 (MQ=255)
                                                                                                 tACCGTACTTTAAATATAACCAAACTTTATTGCATTTAAAATATAAATGAAGATTAAGGAGGTCGCGAAAAGTGATTTTTGTCTTTTCAAGAAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGcc                                       <  2:2529243/140‑1 (MQ=255)
                                                                                                 tACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCTTTTCTTTATTTAAGCCATAATAAAAACGcc                                       <  2:3741762/140‑1 (MQ=255)
                                                                                                  aCCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGcc                                       >  1:4786483/1‑139 (MQ=255)
                                                                                                   ccGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGTATCAATCTAAATTTTCCGCCGTTTCTTTATTTATGCCATAATAAAAActcctc                                     >  2:3833888/1‑140 (MQ=255)
                                                                                                    cGTACGTTAAATATAACCCAACTTTATGGCATTTACAATATAACTGAAGATGAAGGAGGTCGCTTAAAGTTAATTTTGTCTTTTCAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCAATAATAAAAACGCCTcc                                    <  1:3566236/140‑1 (MQ=255)
                                                                                                    cGTACCTTAAATATCACCCAACTTTATGGCATTTACAATATAAATTAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAACCAAACTAAATTTTCCGCCGTTTTTTTATTTAAGCAATAATAAAAACGCCTcc                                    <  1:3597394/140‑1 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGTATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTccc                                   >  1:657383/1‑140 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCa                                                    <  1:2419608/123‑1 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCGTccc                                   >  2:3770407/1‑140 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTccc                                   >  1:2305504/1‑140 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTccc                                   >  1:1767698/1‑140 (MQ=255)
                                                                                                     gTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTccc                                   >  1:4880422/1‑140 (MQ=255)
                                                                                                      tACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCt                                  >  1:4967785/1‑140 (MQ=255)
                                                                                                      caCGTTAAATAAACCCAAATTTTATTCAATTAACAATAAAAATTAAGATGACGGAGGTCGCTTAACGTCATTTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAACACGCCTCCCt                                  <  1:4408267/139‑1 (MQ=255)
                                                                                                           tAAATATAACCCATCTTTATTGCATTTACAATATAAATGAATATGAAGGAGGTGGCGTAACTTGAATTTTGTCTTTTGAAGCAATATAAATTTTCCGCCGTTTATTTATTTAAGCCATAATAAAAACGCCTCCCTTAACg                             <  1:1424587/140‑1 (MQ=255)
                                                                                                           tAAATATAACCCAATTTTATTGATTTTAAAATATAAATTACGATGATGTTGGTCGCGTAAAGTGATTTTTTTCTTTTTAAGAAATCTAATTTTTCCGCCTTTTATTTATTTAAGCAATAATAAAAACGCCTCCCTTAACg                             <  1:439653/140‑1 (MQ=255)
                                                                                                            aaaTATAACCCAAGTTTAGTCCATTAAAAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTTAATTTTTTCTTTTGAAGCAATATAAATTTTCCGCCGTTTCTTTATTTAACCCATAATAAAAACGCCTCCCTTAACGa                            <  1:3231496/140‑1 (MQ=255)
                                                                                                            aaaTATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTATTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGa                            <  2:728628/140‑1 (MQ=255)
                                                                                                            aaaTAAAACCCATATTATTTGCTTTTACATTTTAAATTAATATTACGTTGTTCGCTTAAATTTAATTTTTTCTTTTTAACCAATCTAAATTTCCCGCCGTTTTTTTATTAAAGCCATAATAAAAACGCCTCCCTTAACGa                            <  1:3461493/140‑1 (MQ=255)
                                                                                                             aaTATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGAt                           >  1:70341/1‑140 (MQ=255)
                                                                                                                 taACCCAACTTTATTCCATTTACAATATAAATGAAGATGACGGTGGTCGCGTAAAGTGATTTTTGTATTTTGAAGCAATCTAAATTTTCCGCCGTTTGTTTATTTAAGCCATAATAAACACGCCTCCCTTAACGATGGTc                       <  1:537448/140‑1 (MQ=255)
                                                                                                                  aaCCCAAACTTTTTTGCATTTAAAATAAAAATGAAGATGAAGGAGGTCGCTTAAAGTTAATTTTGTCTTTTCAAGAAATCTAAATTTTCCGCCTTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTc                       <  1:3407837/140‑1 (MQ=255)
                                                                                                                  aaCCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAATTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCAATAATAAAAACGCCTCCCTTAACGATGGTCt                      >  1:3159679/1‑140 (MQ=255)
                                                                                                                   aCCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTa                     >  2:4426926/1‑140 (MQ=255)
                                                                                                                        caCTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATATTGTCTTTTTAAGCAATCTAAATTTTCCGCCGTTTATTTATTTAAGCAATAATAAAAACGCATCCCTTAACTATGTTCTATCGc                 >  2:3880697/2‑140 (MQ=255)
                                                                                                                        aaCTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGc                 >  2:1042907/1‑140 (MQ=255)
                                                                                                                         acttgtttGAATTTACAATATAATTTAAGATGACGGTGGTCGCTTACAGTCAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAACCCATAATAAACACGCCTCCCTTAACGATGGTCTATCGCt                <  1:491270/134‑1 (MQ=255)
                                                                                                                         aCTTTATTGAATTTAAAATATAAATTAAGATGACGGTGGTGGCTTAACGTGATTTTTTTTTTTTGACGCAATATAATTTTTCCGCGTTTTCTTTATTTAAGCAATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCt                <  1:2619376/140‑1 (MQ=255)
                                                                                                                          cTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTa               >  1:2098539/1‑140 (MQ=255)
                                                                                                                               ttGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGaaa          >  2:2383413/1‑140 (MQ=255)
                                                                                                                               ttGCATTTAAAATATAAATTAAGATGAAGTCGGTGGCGTAAAGTCAATTTTGTCTTGTGAAGCAATGTAAATTTTCCCCCGTTTCTTTATTTAACCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGaaa          <  1:1637616/140‑1 (MQ=255)
                                                                                                                               ttGCATTTAAAATATAAATGAAGATGAAGGAGGTCGCGTAATGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCAAAAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGaaa          <  1:2923273/140‑1 (MQ=255)
                                                                                                                                tGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGaaaa         >  2:4704622/1‑140 (MQ=255)
                                                                                                                                tGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGaaaa         >  2:610543/1‑140 (MQ=255)
                                                                                                                                  cATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGAAAAGc       >  1:901854/1‑140 (MQ=255)
                                                                                                                                    tttACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGAAAAGCGt     >  1:4578144/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GGTACGAATATTGCCCTTGCTGCTACTCCAATTTCATGAAGAATCTTCCTCATCATCAACTCCTCAAATGTATTTTTTATTGCAAATGCAATAAAAATACCGTACGTTAAATATAACCCAACTTTATTGCATTTACAATATAAATGAAGATGAAGGAGGTCGCGTAAAGTGAATTTTGTCTTTTGAAGCAATCTAAATTTTCCGCCGTTTCTTTATTTAAGCCATAATAAAAACGCCTCCCTTAACGATGGTCTATCGCTAAGAAAAGCGTCAT  >  NZ_CP034943/3111297‑3111570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: