Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,268,725 C→A 7.1% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,268,7250CA 7.1% 529.0 / 4.4 197intergenic (+73/+137)catR/EO946_RS16920catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (36/139);  new base A (20/2);  total (56/141)
Fisher's exact test for biased strand distribution p-value = 1.00e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACATGGGCT  >  NZ_CP034943/3268586‑3268861
                                                                                                                                           |                                                                                                                                        
ggCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTGTTTTTTTTTGATTTTGCAGGTAAAAAAc                                                                                                                                          >  1:2816918/1‑140 (MQ=255)
ggCCCCCATCTTGGGTGAGATGTCTAACTGGGCGACGGATTGGATTGACCCTTCCGTTCTTGACTAAGTGCTAAAACGGTGCTGATCTCAATGGCGAGAACAGAGCCGTTGTTTTAGTTTTCATTTGCCCGGTGAAAAAc                                                                                                                                          >  1:1859967/1‑140 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCACAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  2:47001/140‑1 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  2:1258023/140‑1 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  2:4016150/140‑1 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  2:250025/140‑1 (MQ=255)
           ttggTGAGATTTCTAAATGGGCGACTGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:425381/138‑1 (MQ=255)
           tggtttagaTTTCTAAATGGGCGAAGGATTGGATTGACCCTTCATTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTGGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:2049111/134‑1 (MQ=255)
           tcggTGAGATTGCTAAATGGGCGACGGATTGGATGGACCCTTCCTTTCTTGACTAATGGCTAACACGGTTCTGATCTCAAAGGCGCGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:3134165/138‑1 (MQ=255)
           tGGGTTAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:2052134/140‑1 (MQ=255)
           tGGGTGAGTTTTCTAATTGGGCGACGTATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:1035548/140‑1 (MQ=255)
           tGGGTGAGATTTGTAACTGTGCTACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAAGGGTTCTGTTCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:1850129/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTGCCAGGTAAAAAACTAATTCTGAGc                                                                                                                               >  1:5165035/1‑140 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTAAAAAACCACTTCTCAGc                                                                                                                               >  1:3147320/1‑140 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:4398467/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:2259800/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:1484675/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:1457610/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:76730/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTACTCAGc                                                                                                                               <  1:3849417/140‑1 (MQ=255)
           tGGGTGAGATTTATAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:3594361/140‑1 (MQ=255)
            gggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGcc                                                                                                                              <  1:539303/140‑1 (MQ=255)
             ggTGAGATTTCTTAAGGGTCTACGGATTGTATTGACTCTTCTTTTCTTGATTTATTTATAAAACGGTTCTTATCTCAAAGGCGAGATAAGTGCGGTTTTTTTATTTTTCATTTGACAGGTAAAAAAAGACTTCTCAGCCg                                                                                                                             <  2:3170276/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGTATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:67324/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTTGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:3054573/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAACACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTGGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:272835/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:4628261/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:2624515/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:1395179/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:1425983/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:3906652/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:2239881/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:2836997/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTATGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:5051303/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGAGTGGATTGACCCTTCCTTTCTTGACTAAGTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:3537004/140‑1 (MQ=255)
             ggTGAGATTTCTAAAGGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:1659285/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:2923495/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:3254977/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:1095939/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:911089/140‑1 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAATAGCCGTTTTTTTATTTTTCAGTTGGCAGGTAAAAAACGACTTCTCAGCCGatat                                                                                                                         >  2:2842623/1‑140 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCAGTTTCGCGGTAAAAAACGACATCTCAGCCGatat                                                                                                                         >  2:3954875/1‑140 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                        <  1:2759374/140‑1 (MQ=255)
                   aTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAAAGAATTCTCAGCCGatatat                                                                                                                       >  2:740475/1‑140 (MQ=255)
                         aaaTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                                 <  2:2335635/140‑1 (MQ=255)
                              ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAcccc                                                                                                            >  2:520034/1‑140 (MQ=255)
                              ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAcccc                                                                                                            >  2:3670544/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAATGGCGAGAAAAGAGCCGTTTTTTTTTTTTTAATTTGCCCGGTAAAAAAAGACGTGTAAGCCGATATATATTATAACCCCCAATTTCGTTTTTAGcc                                                                                           >  1:2276719/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGAATTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGcc                                                                                           >  2:3803393/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAAAGACTTCTCAGCCGATATATAGTAGAACCCCACCTTGCGTTTTTAGcc                                                                                           >  2:4303378/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTGTTTTATTTTTGCTTTGGCCGGTAAAAAACGACGTCTCAGGCGCGATATAGTGTTACCCCACAGTGGGTTTTTatgc                                                                                           >  1:3826216/1‑137 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTCATTTTCCAGGTAAAAAAAGACTTATACGCCGGTATATATTAGAAACCCACAATTCGTTTTTAgac                                                                                           >  2:3933816/1‑138 (MQ=255)
                                                 ccgtccttTATTGACTAATTGCTAAAACGGTTCTGATCTCAAAGCCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCga                                                                                         <  2:4721374/137‑1 (MQ=255)
                                                 ccgaccttACTTCACTAATTGCTAAACCGGTTCTCATCTCAAAGCCGAGAAAAGAGCCGTTTTTTTATTTTTCAGTTGCCACGTAAAAAACGACTTCTCAGCCGAGATATAGTAGAACCCCACATTGCGTTTTTAGCCga                                                                                         <  1:4325347/136‑1 (MQ=255)
                                                   ttccttTCTTGACTAATTGCTAACACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCgaag                                                                                       <  1:4706986/140‑1 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTGAATTTGCAGGTAAAAAAAGACTTCTACGCCGAGATATAGTAGAACCCCACAGTGCGTTTTTATCCgcaga                                                                                      >  1:87742/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTGCCGGTTAAAAACGACATCTCCGCCGATATATAGTAGTACCCCACAATTCGTTTTTAGCCgaaga                                                                                      >  1:3791930/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTTGCAGGTAAAAAAACACTTATCAGCCCCGATAAAGTATAACCCCACAATTCGTTTTTATGCgaaga                                                                                      >  1:3446856/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTTCCACGTAAAAAAACACTTATCAGCCGATATATATTAGAAACCCACATTGCGTTTTTAGCCgaaaa                                                                                      >  1:1286705/1‑138 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGGAAAAAACGACGTATAAGCCGATATATATTATAACCCCAACTTGCGTTTTTAGCCgaaga                                                                                      >  2:2105074/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTGATTTTGCAGGTAAAAAAAGACATCTCAGCCGATATATATTAGAACCCCACATTGGGTTTTTTTCCgaaga                                                                                      >  1:385960/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTGATTTGGCAGGTAAAAAACGACTTCTGAGCCGCTATATTGTAGAACCACACAATTCGTTTTTTTCCgaaga                                                                                      >  1:1717067/1‑140 (MQ=255)
                                                              aCTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGagcag                                                                            <  1:1159694/140‑1 (MQ=255)
                                                                     gCTAAAACGGTTCTGCTCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCCTTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGa                                                                     <  1:3980818/140‑1 (MQ=255)
                                                                      cTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGaa                                                                    <  1:5115931/140‑1 (MQ=255)
                                                                      cTAAAACGGTTCTGATATAAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTATCCGAAGAATTTGAGCAGCTGGAGaa                                                                    <  2:4454206/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCTAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                          <  1:794484/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                          <  2:658854/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACGTCTCAGCCGATATATATTAGAACCCCACATTGCGTTTTTATCCGCAGAATTTGTGCACCTGGCGAAAGAGCTTCAt                                                          >  2:1735592/1‑140 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAAAGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                          >  2:1025975/1‑140 (MQ=255)
                                                                                    aTCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTAAAAAACGACTTCTCAGCCCATATATAGTAGAACCCCACATTGCGTTTTTATCCGAAGAATTTGAGAAGCTGGCGCAAGAGCGTCAAgaga                                                      >  2:1526300/1‑140 (MQ=255)
                                                                                      ctcCAAGGCGACAAAAGAGCCGTTTTTTAATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaag                                                    <  1:4217164/140‑1 (MQ=255)
                                                                                      ctcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaag                                                    <  1:1755663/140‑1 (MQ=255)
                                                                                       tcAAAGGCGAGAAAAGAGGCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACCTTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  2:33720/140‑1 (MQ=255)
                                                                                       tcAAAGGCGAGAAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  2:1826153/140‑1 (MQ=255)
                                                                                       tcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  1:197774/140‑1 (MQ=255)
                                                                                         aaaGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCCGGTAAAAAACGACTTCTCAGCCGATATATATTAGAACCCCACAATGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAACATg                                                 >  2:4111332/1‑140 (MQ=255)
                                                                                            ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                              <  1:2097962/140‑1 (MQ=255)
                                                                                            ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTGGCCAGGTAAAAAACGACGTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                              <  1:4668279/140‑1 (MQ=255)
                                                                                              cGAGAAAAGAGCCGTTTTTTTATTTGTCATTTGGCAGGTAAAAAACGACTTCACAGCCCATATATAGTAAAAACCCACATTGCGTTTTTAGCCCAAAAATTTGAGCATCTGTCGAAACACCTAGATTAGAAAATGTATCa                                            >  2:3611740/1‑140 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATTTATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  2:4655522/140‑1 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACCTTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  2:3826216/140‑1 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  1:842348/140‑1 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  2:1718227/140‑1 (MQ=255)
                                                                                                      gagCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAt                                    <  2:5082251/140‑1 (MQ=255)
                                                                                                       agCCGTTTTTTTATTTTTGATTTTCCAGGTAAAAAACGACTTCTCAGCCGATATATATTATTAACCCAAATTGCGTTTTTATCCGAAGAATTTTAGCAGCTTGCGAAATAGCTTCATGAGAAAATGGATAAGTTCACAtt                                   >  2:4507893/1‑140 (MQ=255)
                                                                                                                      tttCATTTGCCCGGTAAAAAACGACTTCTCAGCCGAGATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  1:156098/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGCCTTCTCAGACGATATATATTAGAAACCCACATTGCGTTTTTAGCAGAAGAATTTGATCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  2:3877603/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  1:1950457/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  1:4776458/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  2:2457796/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc                   <  1:520034/140‑1 (MQ=255)
                                                                                                                           tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa               <  2:963702/140‑1 (MQ=255)
                                                                                                                           tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa               <  1:760062/140‑1 (MQ=255)
                                                                                                                           tttGCCAGGTAAAAAACGACTTCTCAGCCCATATATAGTACAACCCCACATGGCGATTATAGCCGAAGAATTTGCGCCGCTGGCGAAAGAGCTTCATGAGAACATGGATCAGTTCACATATAACATAACCCACGTCCAGa               <  2:2402153/140‑1 (MQ=255)
                                                                                                                                  ggTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACa        <  2:3446856/140‑1 (MQ=255)
                                                                                                                                  ggTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACa        <  2:1286705/140‑1 (MQ=255)
                                                                                                                                   gTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAt       <  1:709365/140‑1 (MQ=255)
                                                                                                                                   gTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAt       <  1:4475078/140‑1 (MQ=255)
                                                                                                                                   gTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAt       <  1:3597471/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACATGGGCT  >  NZ_CP034943/3268586‑3268861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: