BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,713	G→A	24.5%	L230L (CTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,713	0	G	A	24.5%	684.8 / 246.3	363	L230L (CTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (154/119); new base A (53/36); total (208/155)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 363 total aligned reads displayed.

AAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285576‑3285785

     tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  1:3808548/1‑140 (MQ=21)

     tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGGCCGTTACTAATTTTTCa                                                                   >  1:2449829/1‑140 (MQ=255)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTCATATAATGGAAAGGTACCATTGTTTCTGTCCATAAGGGAAATATGTGATGTCCGTAACGAGTTTTTCAag                                                                 >  2:5165242/1‑140 (MQ=14)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCACATAGGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:1242243/1‑140 (MQ=255)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:1426854/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:4155903/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGAGGTCCGTTACTAGTTTTTCAag                                                                 >  2:1796745/1‑140 (MQ=255)

         tAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAATCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAagag                                                               <  1:756717/140‑1 (MQ=255)

            gACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGTCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGc                                                            >  1:2122072/1‑140 (MQ=255)

            gACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGc                                                            >  2:4619877/1‑140 (MQ=40)

               aaaGTCTGTGTCCTGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                         <  1:1785791/140‑1 (MQ=255)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                         <  1:4292546/140‑1 (MQ=40)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  1:2385027/140‑1 (MQ=21)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  1:467570/140‑1 (MQ=21)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:3216442/1‑140 (MQ=21)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  1:333718/1‑140 (MQ=21)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATAAAATATACTGGAAAGGTACAATTGTTTCTGTCAATAATAAAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGTGCGAtaa                                                       >  2:795292/1‑138 (MQ=11)

                  gTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAg                                                      <  1:2050647/140‑1 (MQ=40)

                   tCTGTGTCCTTCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGa                                                     <  2:2150244/140‑1 (MQ=255)

                       tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  2:357835/1‑140 (MQ=21)

                       gggccTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCCGTGTATACCTCTAATATACTGGAAAGGTACAATTGTTTCTGTCCCTCCGGCAGATAGGTGCGGGCGGTGACTCGTGTTTCGTGAGGGCGCTCAGACGGa                                                 >  2:4072050/4‑140 (MQ=9)

                           cTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGt                                             >  1:1969819/1‑140 (MQ=21)

                            tGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGtt                                            >  2:1943922/1‑140 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:4502142/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:4145510/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:384518/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:1093441/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  2:3082154/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:4649066/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  1:3490943/1‑140 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  1:836235/1‑140 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  2:3679893/1‑140 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  2:2324673/1‑140 (MQ=21)

                              cTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGTAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTcc                                          <  1:93169/140‑1 (MQ=255)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTTTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATTTCATTTATTTGTTTTTCTATATGTCTATCAGAGTGTAAGttatg                                         <  1:25625/140‑4 (MQ=14)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:2812565/140‑1 (MQ=40)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         >  2:4668552/1‑140 (MQ=21)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  1:1823155/140‑1 (MQ=21)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:4030553/140‑1 (MQ=21)

                               ttATCCCCAATCCTAAAAGAAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:3355853/140‑1 (MQ=255)

                                tATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGa                                        <  1:1913910/140‑1 (MQ=39)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  1:2944878/1‑140 (MQ=38)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  2:4875774/1‑140 (MQ=38)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  2:1671247/1‑140 (MQ=38)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  2:1192310/1‑140 (MQ=38)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGACAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  2:2463977/1‑140 (MQ=16)

                                  tCCCCAATCGTAAAAGCAATCTCTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  2:84000/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCTCTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:309581/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  2:2334007/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  2:1519649/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:719223/140‑1 (MQ=17)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  1:1401491/1‑140 (MQ=14)

                                    cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  2:3596027/140‑1 (MQ=35)

                                    cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  1:592903/140‑1 (MQ=11)

                                    cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  1:3185299/140‑1 (MQ=11)

                                      cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  >  1:4095250/1‑140 (MQ=34)

                                      cAATCCTAACAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:2700067/140‑1 (MQ=255)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCGGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:1697341/1‑140 (MQ=21)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:3251421/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:3633398/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:2975392/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:2657429/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:290940/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:2056257/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:4353075/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:4450618/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:498411/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:5027346/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  2:718096/1‑140 (MQ=34)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGGTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:266563/1‑140 (MQ=21)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTCATGTCCGTTACTAGTTTTACAAGAGGGCGATCACACTGAAAGTTCCGATCCAatat                                >  2:3915340/1‑140 (MQ=255)

                                            tAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtatta                            >  2:1049920/1‑140 (MQ=32)

                                                aGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGa                        <  1:4027056/140‑1 (MQ=32)

                                                 gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAc                       <  1:1642960/140‑1 (MQ=32)

                                                 gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAc                       <  1:1031639/140‑1 (MQ=32)

                                                   aaTCCCGTCTCCATTGTATACAGCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:2889058/140‑1 (MQ=12)

                                                   aaTCACTTCTCCCTTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:915558/140‑1 (MQ=17)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATACGGCAACTATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:4519431/140‑1 (MQ=17)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATTTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:4720513/140‑1 (MQ=17)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:3506195/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:586625/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:4877117/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:4784715/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:2270934/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:4613368/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:3782715/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:351110/140‑1 (MQ=32)

                                                    aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGTTCCACTATATTATCGACCAc                    >  2:4285554/1‑140 (MQ=17)

                                                     tCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACg                   >  1:1162597/1‑140 (MQ=32)

                                                           ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCatat             >  1:2096021/1‑140 (MQ=32)

                                                           ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCatat             >  1:5182262/1‑140 (MQ=32)

                                                           ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCatat             >  1:1541225/1‑140 (MQ=32)

                                                              cATTGTATACATCTAATATACTGTCAAGTTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGGCCGTTACTAGTTTTTCAATAGGCCGATCAGACTGAAAGTTCCGAGCCAATATATTATAGACCACTGCATATGGc          >  2:2489350/1‑140 (MQ=11)

                                                               ataagagaCAGCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCt         <  2:3310658/133‑1 (MQ=2)

                                                                 tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  1:4383690/1‑140 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc     >  1:5134209/1‑140 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTACAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc     >  2:1613956/1‑140 (MQ=17)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt   >  1:560906/1‑140 (MQ=32)

                                                                      aCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCCtt  >  1:1732477/1‑140 (MQ=14)

                                                                      aCATCTAATATACTGCAAAGGTACAATTGTTTCTGTCCATAAGGAACATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCACTATATTATCGACCACGGCATATGGCTGCCCCtt  >  1:5059066/1‑140 (MQ=11)

AAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285576‑3285785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`