Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,319,721 T→G 5.2% pseudogene (584/1292 nt) EO946_RS17170 ← arabinogalactan endo‑beta‑1,4‑galactanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,319,7210TG 5.2% 847.8 / 5.8 315pseudogene (584/1292 nt)EO946_RS17170arabinogalactan endo‑beta‑1,4‑galactanase
Reads supporting (aligned to +/- strand):  ref base T (113/175);  new base G (24/2);  total (137/178)
Fisher's exact test for biased strand distribution p-value = 8.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

CCTTCCTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTC  >  NZ_CP034943/3319583‑3319859
                                                                                                                                          |                                                                                                                                          
cctgcctgCCGTTTCAGGATTGGTAAACTGCAGTGCGACCAAAATCCCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATGTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGttt                                                                                                                                           <  2:2857901/140‑1 (MQ=255)
ccTTCCTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGttt                                                                                                                                           <  2:2981456/140‑1 (MQ=255)
ccTTCCTGCAGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGttt                                                                                                                                           <  2:4722551/140‑1 (MQ=255)
   tCCTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCATGTCGTTTCAt                                                                                                                                        >  2:2064418/1‑140 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  1:2619282/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:1410195/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:3413597/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:2240724/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:1077844/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:3928364/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  2:3140116/140‑1 (MQ=255)
     cTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCGTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAttt                                                                                                                                      <  1:4950580/140‑1 (MQ=255)
      tGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTc                                                                                                                                     <  1:4501410/140‑1 (MQ=255)
      tGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTc                                                                                                                                     <  1:4620449/140‑1 (MQ=255)
       gCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTcc                                                                                                                                    <  2:1318003/140‑1 (MQ=255)
           tttCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCGTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCCAcc                                                                                                                                >  1:2365528/1‑140 (MQ=255)
               aGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGaa                                                                                                                            <  2:1728498/140‑1 (MQ=255)
               aGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTCCAAATGCCCCTGTCGTTTCAATTCCGACCTGaa                                                                                                                            >  2:3062570/1‑140 (MQ=255)
               aGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTCCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGaa                                                                                                                            <  2:2532973/140‑1 (MQ=255)
                 gATTGGTAAAATGCAGTGCGACCACAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAcc                                                                                                                          <  1:2909045/140‑1 (MQ=255)
                 gATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAcc                                                                                                                          <  1:60729/140‑1 (MQ=255)
                      gTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGcc                                                                                                                     <  2:1519008/140‑1 (MQ=255)
                       tAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCa                                                                                                                    <  2:1364572/140‑1 (MQ=255)
                           aTGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCACTGTCGTTTCATTTCCGACCTGAACCATGCCAATGt                                                                                                                >  1:1383084/1‑140 (MQ=255)
                            tGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTc                                                                                                               <  2:1555673/140‑1 (MQ=255)
                              cAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGa                                                                                                             <  1:4813071/140‑1 (MQ=255)
                                gTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGGTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAAAGTCGACg                                                                                                           >  1:4325275/1‑140 (MQ=255)
                                gTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACg                                                                                                           >  2:70668/1‑140 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGc                                                                                                          >  1:3549223/1‑140 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGc                                                                                                          >  1:2752717/1‑140 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGc                                                                                                          <  2:381371/140‑1 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGc                                                                                                          >  1:2107114/1‑140 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTACGACCTGAACCATTCCAATGTCGACGc                                                                                                          >  2:4849186/1‑140 (MQ=255)
                                 tGCGACCAAAATACCTGGGTCCGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGc                                                                                                          >  2:4406434/1‑140 (MQ=255)
                                     aCCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGccttc                                                                                                      <  2:2752717/140‑1 (MQ=255)
                                     aCCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGccttc                                                                                                      <  2:2331253/140‑1 (MQ=255)
                                      ccAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAATTCCGACCTGAACCAAGCCAATGTCGACGccttcc                                                                                                     >  1:3180634/1‑140 (MQ=255)
                                               cTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatcatc                                                                                            <  2:4063928/140‑1 (MQ=255)
                                                tGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCCATGTCGACGCCGTCCGTGATCATCt                                                                                           >  1:3937294/1‑140 (MQ=255)
                                                 gggTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTACGACCTGAACCAGGCCCAAGTCGACGCCGTCCGTGATCATCtt                                                                                          >  1:767638/1‑140 (MQ=255)
                                                  ggTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCCAGGTCGACCCCTTCCTTGATCATCttt                                                                                         >  2:3143698/1‑140 (MQ=255)
                                                     ctgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGc                                                                                      <  2:5105782/140‑1 (MQ=255)
                                                      tgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTgga                                                                                     >  2:4624194/1‑138 (MQ=255)
                                                      tgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCa                                                                                     <  2:3593995/140‑1 (MQ=255)
                                                        tctcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAGTTCCGACCTGAACCATGCCCATGTCGACGCCTTCCTGGATCATCTTTTTCAgg                                                                                   >  1:3589863/1‑140 (MQ=255)
                                                          tcCCTGACCGCGCTGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                 <  2:2926913/140‑1 (MQ=255)
                                                            ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                               <  2:3549223/140‑1 (MQ=255)
                                                            ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTACCACCTGAAAAATACCAATGTCTACGCCTTACTTGATAATCTTTTGCAGGCttt                                                                               >  1:872139/1‑140 (MQ=255)
                                                             cTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTg                                                                              >  2:1994861/1‑140 (MQ=255)
                                                              tGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGt                                                                             <  1:504260/140‑1 (MQ=255)
                                                              tGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGt                                                                             <  1:4869806/140‑1 (MQ=255)
                                                               gACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATTCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGtt                                                                            >  2:2727710/1‑140 (MQ=255)
                                                               gACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCGTTTGCAGGCTTTGtt                                                                            >  2:3361421/1‑140 (MQ=255)
                                                               gACCGCGCGGCGTCCTTCATTAAATAATTGGCTCAGTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATCTCGACCCCTTCCGTGATCCTCATTTGCAGGCgttgtt                                                                            >  2:3716218/1‑140 (MQ=255)
                                                                  cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCg                                                                         <  1:3724079/140‑1 (MQ=255)
                                                                  cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCg                                                                         >  1:908923/1‑140 (MQ=255)
                                                                  cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAACATGCCCATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCg                                                                         >  2:829035/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       <  1:2281037/140‑1 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       >  2:1610048/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       <  1:4463315/140‑1 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       <  1:1771923/140‑1 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       >  2:4500428/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAAATTCGACGCCCTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       >  1:2473914/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTAATTTCCGACCTGAAACATGCCAATGTCGACGCCTTCCGTGCTCAACTTTTTCAGGCTTTGTTTCGta                                                                       >  1:3125762/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTAATTTACGACCTGAACCAATCCAAATTAGAAGCCTTCCTTGATAATATTTTTAACGCTTTGTTTAGta                                                                       >  1:3270826/1‑140 (MQ=255)
                                                                    cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAAGGTCGTCGCCTTCCGTGATGCTCTTTTGCAGGCGGTGGTTCgga                                                                       >  2:4999591/1‑138 (MQ=255)
                                                                    cgcgGCTTACTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                       <  1:1601733/140‑1 (MQ=255)
                                                                     gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                      <  1:3820639/140‑1 (MQ=255)
                                                                      cgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCAAGCCAATGTCCACGCCTTACTTGATCATATTTTGGAGGCTTTTTTTCGtcta                                                                     >  1:5143097/1‑140 (MQ=255)
                                                                                      ataatTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                      >  1:3011338/1‑123 (MQ=255)
                                                                                           tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCGTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGttttttt                                                <  2:4919293/140‑1 (MQ=255)
                                                                                           tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGttttttt                                                <  1:1206449/140‑1 (MQ=255)
                                                                                           tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGttttttt                                                <  1:1960422/140‑1 (MQ=255)
                                                                                           tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATACCCCTGTCGTTTCATTTc                                                                                                                                     >  2:4035985/1‑55 (MQ=255)
                                                                                            ggCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGCACCATGCCAATGTCGACGCCTTCCTTGATCATCTTGTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTa                                               <  1:70668/140‑1 (MQ=255)
                                                                                               tCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCg                                                                         >  1:5171462/1‑111 (MQ=255)
                                                                                                cagtttCGCACAATCGGTTTCACCTGCAAATGCCGCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGATTTGTTTCGTATACTCCTATAGCTTTGTTTTTTTAGCtt                                           <  1:1417665/137‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:58103/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:1266517/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:4118680/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:4177672/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTCGTTTTTTTAGCtt                                           <  2:522710/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCAGGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:4356197/140‑1 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCACCTGAACCATGCCAATGTCGACGCCTTCCTTGatc                                                                                               >  2:3649865/1‑87 (MQ=255)
                                                                                                cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTACGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                           <  2:212425/140‑1 (MQ=255)
                                                                                                   tttCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGTCCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATGTTTTGCAGGCTTTGTTTCGTATACTCATAGAGGTTTGTTTTTTTAGCTTCaa                                        >  2:2434464/1‑140 (MQ=255)
                                                                                                        cccAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCAGAACCATGCCAATGTCGACGCCTTCCTTGATCCTCTTTTGCAGGCGTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTg                                   >  2:954147/1‑140 (MQ=255)
                                                                                                             tCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTACGACCGGAACCAAGCCCAGGTCGACGCCGTCCGTGATCATGTTTTTCAGGCGTTGTTTGGTTTACTCATAGAGGTTTGTTTTTTTTTCTTCAAAGCTGAGCtt                              >  1:12875/1‑140 (MQ=255)
                                                                                                             tCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCCATGTCGACGCCTTCCGTGATGATGTTTTGCAGGCTTTGTGTGGTATACTCCTAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCtt                              >  2:4606510/1‑140 (MQ=255)
                                                                                                             tCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCGTGCTCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCGTTGTTTTTTTAGCTTCAAAGCTGAGCtt                              >  2:4456097/1‑140 (MQ=255)
                                                                                                             tCGGTTTCACCTGCAAATGCCCCTGTCGTGTGATTTCCGACCTGAACCATGCCAATTTCGACGCCGTCCTTGATCATCTTTTGCAGGCTTTGTTTGGTATACTGATAGAGGGTTGTTTTTTTATCTTCTAAGCTGGGCtt                              >  1:1094581/1‑140 (MQ=255)
                                                                                                                    cACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCaa                       <  2:261869/140‑1 (MQ=255)
                                                                                                                     aCCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCACTGTCGACGCCTTCCTGGATCATCGTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAg                      >  2:3763324/1‑140 (MQ=255)
                                                                                                                     aCCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTGCTTGATGATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAg                      >  2:3199054/1‑140 (MQ=255)
                                                                                                                     aCCGGCAAATGCCCCTGTCGTGTCATTGCCGACCTGACCCATGCCAATGTCGACGCCTTCCTTGATCATCTTTGGCAGGCTTTGTTACGTCTACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAg                      <  2:1161557/140‑1 (MQ=255)
                                                                                                                       cTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCt                    <  1:471914/140‑1 (MQ=255)
                                                                                                                        tGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCtt                   <  1:4223966/140‑1 (MQ=255)
                                                                                                                        tGCAAATGCCCCTGTCGTTTCATTTCCGAACTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCtt                   <  1:2015906/140‑1 (MQ=255)
                                                                                                                        tGCAAATGCCCCTGTCGTTTCATGTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTACAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCtt                   <  1:100374/140‑1 (MQ=255)
                                                                                                                           aaaTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTgg                <  1:985943/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CCTTCCTGCCGTTTCAGGATTGGTAAAATGCAGTGCGACCAAAATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTC  >  NZ_CP034943/3319583‑3319859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: