Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 361,366 C→A 8.1% Q152K (CAG→AAG)  EO946_RS01960 → FAD/NAD(P)‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943361,3660CA 8.1% 954.4 / 14.4 405Q152K (CAG→AAG) EO946_RS01960FAD/NAD(P)‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (164/185);  new base A (51/2);  total (218/187)
Fisher's exact test for biased strand distribution p-value = 2.85e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 423 total aligned reads displayed.

TGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGGCTGCTCATTACGC  >  NZ_CP034943/361227‑361505
                                                                                                                                           |                                                                                                                                           
tGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGAGTTTTTatcatc                                                                                                                                             >  2:1839170/1‑140 (MQ=255)
tGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcatc                                                                                                                                             >  2:1586776/1‑140 (MQ=255)
tGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcatc                                                                                                                                             >  2:3827643/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGTCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGGTTTAGCCATGAGTTTTTATAATAAGc                                                                                                                                          >  1:2637147/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTGGTGTTTGGCATGGGGTTTTCTCTTGAGc                                                                                                                                          >  2:4710486/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGc                                                                                                                                          >  2:2920172/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAGTGTTTTTTGTCATCAGc                                                                                                                                          >  1:1263068/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGc                                                                                                                                          >  2:3736317/1‑140 (MQ=255)
   aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCAATAGTTTTTCTCATCAGc                                                                                                                                          >  2:3918588/1‑140 (MQ=255)
        atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATAAGCcttct                                                                                                                                     >  1:4749908/1‑140 (MQ=255)
        atatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCcttct                                                                                                                                     >  1:3188130/1‑140 (MQ=255)
         tatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTACCCATGAGTTTTTCTCCTCCGccgtagt                                                                                                                                    >  2:2367610/1‑135 (MQ=255)
         tatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCcttctt                                                                                                                                    >  2:5025063/1‑140 (MQ=255)
          atGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCACCCGTCTTg                                                                                                                                   >  1:2077656/1‑140 (MQ=255)
             gATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCCAGAGTTTTTGTCATAAGCCTTCTTGGcc                                                                                                                                >  1:1781800/1‑140 (MQ=255)
              aTCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTTGCCg                                                                                                                               >  1:1072770/1‑140 (MQ=255)
               tCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCATGAGTTTTTCTCATCAGCCGTCTTGGCCgg                                                                                                                              >  1:3620233/1‑140 (MQ=255)
               tCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTGTCATCAGCCTTCTTTGCCgg                                                                                                                              >  1:4835263/1‑140 (MQ=255)
                cAAGCATGCAATAAAGCCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGCAGACAGATACCGCTGGGACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGa                                                                                                                             <  2:4301223/140‑1 (MQ=255)
                  aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTCTCATAAGCCTTCTTGGGCGGAGt                                                                                                                           >  1:4432771/1‑140 (MQ=255)
                  aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATCAGCCGTCTTTGGCGGAGt                                                                                                                           >  1:1504432/1‑140 (MQ=255)
                  aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGcc                                                                                                                                         >  1:2358090/1‑126 (MQ=255)
                       ggAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATAATAAGCCTTCTTTGGCGGAGTGGGCa                                                                                                                      >  1:1640505/1‑140 (MQ=255)
                       ggAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATGACCCGTCTTTGCCGGCGTGGGcc                                                                                                                      >  1:3981618/1‑139 (MQ=255)
                        gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCCTGAGTTTTTCTCATCACCCTTCTTTGCCCGACTGGGcca                                                                                                                     >  2:1016864/1‑138 (MQ=255)
                        gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCCTGAGTTTTTGTCATAACCCGTCTTTGCCGGCGTGGGccc                                                                                                                     >  1:1556956/1‑138 (MQ=255)
                           aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                                  <  1:2172470/140‑1 (MQ=255)
                           aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                                  <  1:773855/140‑1 (MQ=255)
                           aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                                  <  1:1443158/140‑1 (MQ=255)
                           aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                                  <  1:1381947/140‑1 (MQ=255)
                             cAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCATCCTTCTTTGCCGGATTGGGCAAAACCa                                                                                                                >  2:2581154/1‑140 (MQ=255)
                             cAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCa                                                                                                                >  2:192669/1‑140 (MQ=255)
                                gCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAttt                                                                                                             <  1:3050537/140‑1 (MQ=255)
                                           aaTTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCgg                                                                                                  <  2:4137024/140‑1 (MQ=255)
                                             tttGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCa                                                                                                <  2:4588431/140‑1 (MQ=255)
                                               tGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCctt                                                                                                                                       <  1:2458795/99‑1 (MQ=255)
                                                     tggCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAt                                                                                        <  2:5142070/139‑1 (MQ=255)
                                                     gggCAAACGGGTTGTGGGAAGCGTAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAt                                                                                        <  2:3338889/140‑1 (MQ=255)
                                                               gTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                                              <  2:4782965/140‑1 (MQ=255)
                                                               gTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                                              <  2:799115/140‑1 (MQ=255)
                                                               gTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                                              <  1:2228857/140‑1 (MQ=255)
                                                                ttGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGGCGGAGTGGGCACAAACATTTCAGAATAGCGGCATTTATAATGTGttttaaag                                                                             >  1:5031824/1‑135 (MQ=255)
                                                                  gtgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGAGTTTTTGTCATAAGACTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATATCGGCATTTATAATGTGTTTTACagat                                                                           >  1:3299891/1‑139 (MQ=255)
                                                                   tgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACagaga                                                                          <  1:1593817/140‑1 (MQ=255)
                                                                   tgGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTGTCTCATCAGCCTTCTGGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATGTATCATGTGTTTGACagaga                                                                          <  1:1945289/140‑1 (MQ=255)
                                                                       aaGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTg                                                                      <  2:4523506/140‑1 (MQ=255)
                                                                        aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                                     <  1:4650332/140‑1 (MQ=255)
                                                                        aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                                     <  1:4167251/140‑1 (MQ=255)
                                                                        aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTGCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                                     <  2:4457246/140‑1 (MQ=255)
                                                                         gCGGAGACAGATACCTCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTGTTGGCATCACCCATCTTGGCCCGAGGGGGCAAAACCATTTAATAAAAGCGGCAATTATAATGTTTATGACAAATAGGTGCa                                                                    >  2:2323370/1‑140 (MQ=255)
                                                                         gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTCAGAATATCCGCATTTATAATGTGTTTTACAGAGAGGTGCa                                                                    >  1:3792668/1‑140 (MQ=255)
                                                                         gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCGTGGCCGGAGTGGGCCAAACCATTTCAGAATAGCGGCCTTTATCATGTGTTTTAAAGATAGGTGCa                                                                    >  2:1133127/1‑140 (MQ=255)
                                                                         gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCATCAGCCTTCTTGGCCGGAGTGGGCAAAAACATTTAAGAATAGCGGCATTTATAATGTGGTTTACAGAGAGTTGCa                                                                    >  1:3507333/1‑140 (MQ=255)
                                                                               aCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCGCATCAGCCTTCTTGGCCGGAGTGGGCCAAACCAATTGAGAATAGCGGCATTTATCATGGGTTTGGCAGAGAGGTGCACGCTGt                                                              >  2:1878310/1‑140 (MQ=255)
                                                                               aCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCCTCAGCCTTCTTGGCCGGAGTGGGCCAAACCATTTCAGAATATCGGCATTTATACTGTGTGTGACCGAGAGGTGCACGATGt                                                              >  2:3021694/1‑140 (MQ=255)
                                                                                cAGATACCTCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGTCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                                             <  1:927073/140‑1 (MQ=255)
                                                                                cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                                             <  1:3836832/140‑1 (MQ=255)
                                                                                   aTACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATCCTCCTTCTTTGCCGGGGTGGGCCAAACCAATTCAGAATATCGGCAATTATCATGTGTTTTACCTAGAGGTGCACGAGGTaaac                                                          >  2:3088509/1‑138 (MQ=255)
                                                                                   aTACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCGTCTTGGCCGGCGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATTTTTTTGACAGATAGGTGCACGATGGAAGc                                                          >  2:3555464/1‑140 (MQ=255)
                                                                                    tACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCGTCTTGGCCGGTGTGGGCAAAAACATTTTAGAATAGCGGCATTTATAAAGTGTTTGACAGAGAGGTGGACGATGTAAGcc                                                         >  2:764045/1‑139 (MQ=255)
                                                                                    tACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGCCGTCTTGGGCGGCGGGGGCAAAACCAATTGAGACTAGCGGCCTTTATCATGTGTTTGTAAGAGAGGTGCCCGATGTAAGcc                                                         >  1:5075549/1‑139 (MQ=255)
                                                                                       cGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATg                                                      <  1:4301424/140‑1 (MQ=255)
                                                                                       cGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATg                                                      <  1:117510/140‑1 (MQ=255)
                                                                                       cGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATg                                                      <  1:3410363/140‑1 (MQ=255)
                                                                                         cTGGTACTCTCAGGGGGGACCGAGTCGTTTTTTCCATGAGTTTTTCTCCACAGCCGTCTTGGCCGGAGTGGGCAAAAACCTTTCAGAATAGCGGCCTTTATCATTGGTTTGACAGAGAGGTGCACGATTTAAGCACTGaa                                                    >  2:1188506/1‑140 (MQ=255)
                                                                                           ggTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTGTCATAAGCCATATTGGCCGGAGTGGGCAAAACCATTTAAGAAAATCGGCATTTATCAAGTTTTTTAACGAGAGGTGCACGATTGAACCAAAGaaac                                                  >  2:2803231/1‑139 (MQ=255)
                                                                                           ggTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGAGTTTTTCTCATCAGCCTTCTTGGCCGGATTTGGCAAAAACATTTAAGAAAAGCGGCATTTATAATGTGTTTGACACAGAGGTGCACGATGTAAGAAATGaaaa                                                  >  2:1703728/1‑139 (MQ=255)
                                                                                           ggTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTGATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTGAGAATAGCGGCATTTATAATGGGGTTGACAGAGAGGTGCACGATGTAAGCAAAGaaat                                                  >  2:1284107/1‑139 (MQ=255)
                                                                                           ggTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGACTTTTTATGATACGCCTTGTTTGCCGGAGTGGGCCAAACCATTTGAGAAAAGCGGCATTTATCATTTTTTTGACAGAGAGGTGGACGATGTAAGCAATGAAAg                                                  >  2:553826/1‑140 (MQ=255)
                                                                                             tACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCCAAACCAATTCAGAATAGCGGCATTTATCATGTTGTTGACAGAGAGGTGCACGATGTAAGCAAAGAAAGTc                                                >  2:639359/1‑140 (MQ=255)
                                                                                                 ctcAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCCAAACCATTTGAGAATAGCGGCATTTATAATGTGTTTGACAGAGACGTGCACGATGTAAGCAATGAAAGTATGCa                                            >  2:2671314/1‑140 (MQ=255)
                                                                                                   cAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTAGCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCaaa                                          <  1:324970/140‑1 (MQ=255)
                                                                                                      gggggAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATgg                                       <  1:2382696/140‑1 (MQ=255)
                                                                                                      gggggAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATgg                                       <  1:4952371/140‑1 (MQ=255)
                                                                                                       ggggAACGAGTCGTTTTAGCAAAGAGTTTTTATCAACAGCCTTCTTGGGCGGAGTGGGCAAAAACATTTAACAATAGCCGCATTTATCATGTGTTTTACCGAGCGGTGCACGATGTAAGAAATGAAAGTCTGCAAATgag                                      >  2:3254704/1‑138 (MQ=255)
                                                                                                         ggAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTATTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATGATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAACTCTGCAAAATGCAg                                    >  2:3948804/1‑140 (MQ=255)
                                                                                                           aaCGAGTCGTTTTAGCCATGATTTTTTATCATCAGCCGTCTTGGCCGGGGTGGGCAAAACCATTTCAGAATATCGGCATTTATCATGTGTTTTTCAGGGAGGTGCACGATGTAAGCAATGAAAGTCAGAAAAAGGGAGtt                                  >  2:5049347/1‑140 (MQ=255)
                                                                                                           aaCGAGTCGTGTTAGCCCTGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAACATTTAAGAAAAGCGGCATTTATAATGTGTTTTACAGAGAGGTGCACGATGTAAGAAAATAAATTCTTCAAATGTCAGtt                                  >  2:2872694/1‑140 (MQ=255)
                                                                                                            aCGAGTCGTTTTAGCCATGAGTTTTTCTCATAAGCCTTCTTGGGCGGAGTGGGCAAAAACATTTGAGAATAGCGGCATTTATAATGTGTTTGTAAGAGAGGTGCACGATGTAAGCAATGAAATTATGGAAATGGGAGTtg                                 >  2:4364734/1‑140 (MQ=255)
                                                                                                             cGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGCAGAGCGGCATTTATCATGTGTTTGCCAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTtgt                                <  2:2493179/140‑1 (MQ=255)
                                                                                                                gTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTggg                             <  2:3438945/140‑1 (MQ=255)
                                                                                                                 tCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGc                            <  1:4543224/140‑1 (MQ=255)
                                                                                                                  cGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCg                           <  1:1882903/140‑1 (MQ=255)
                                                                                                                    ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg                         <  1:2932725/140‑1 (MQ=255)
                                                                                                                    ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg                         <  2:1607540/140‑1 (MQ=255)
                                                                                                                    ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg                         <  2:1527213/140‑1 (MQ=255)
                                                                                                                         gCCCTGAGTTGTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGAt                    <  1:4358548/140‑1 (MQ=255)
                                                                                                                         gCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGAt                    <  2:2115289/140‑1 (MQ=255)
                                                                                                                         gCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGCGCAAAACCATTTCAGAAGAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGAt                    <  1:582527/140‑1 (MQ=255)
                                                                                                                             tGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACg                >  2:4946669/1‑140 (MQ=255)
                                                                                                                              gagtggttCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTGGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACgg               <  2:1436725/134‑1 (MQ=255)
                                                                                                                              gAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACgg               >  2:4179936/1‑140 (MQ=255)
                                                                                                                              gAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACgg               >  1:3769527/1‑140 (MQ=255)
                                                                                                                              gAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACgg               >  2:1346833/1‑140 (MQ=255)
                                                                                                                               aGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGgc              >  1:3902401/1‑140 (MQ=255)
                                                                                                                                 tttttCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGgctg            <  2:3624632/140‑1 (MQ=255)
                                                                                                                                   tttCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGgctgct          >  1:1024245/1‑140 (MQ=255)
                                                                                                                                   tttCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGgctgct          >  2:1064841/1‑140 (MQ=255)
                                                                                                                                        catcaGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGGCTGCTCATTa     <  1:814556/140‑1 (MQ=255)
                                                                                                                                           caGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGGCTGCTCATTACGc  >  1:2825389/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATAACGGCTGCTCATTACGC  >  NZ_CP034943/361227‑361505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: