Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,349,666 T→G 6.9% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,349,6660TG 6.9% 756.7 / 4.1 342intergenic (+1/+26)EO946_RS17305/padChypothetical protein/phenolic acid decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (190/109);  new base G (20/22);  total (210/132)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804
                                                                                                                                           |                                                                                                                                          
       tCGGCCGTGTTATGTTTACGGTACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTATCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGCAAAGAGTAGTGATTCTCttt                                                                                                                                     >  2:3111562/1‑140 (MQ=255)
          gCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTcc                                                                                                                                  <  1:3171502/140‑1 (MQ=255)
          gCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTGTcc                                                                                                                                  <  1:3360897/140‑1 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATCCTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTGTCCGGGTTTTTATGttt                                                                                                                    >  1:1703194/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  2:2160065/1‑140 (MQ=255)
                          gggACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTttat                                                                                                                  <  1:1442629/140‑1 (MQ=255)
                          gggACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTttat                                                                                                                  <  1:2665800/140‑1 (MQ=255)
                             acacAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTtattat                                                                                                               >  1:2629608/1‑140 (MQ=255)
                              aacaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTAGGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGGGAGTCGCTTGTGCCGGTTGTTATGTGTGTtata                                                                                                              >  2:3871194/2‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                           >  2:4809154/1‑140 (MQ=255)
                                    cTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:440402/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATCCTCTTTTTCGCGGTAATTATGATGGGTCCAGCATACTCAGAAAAACGAAAACCCGGGATAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:3014753/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:418295/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:4272601/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:2119674/140‑1 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCCTCATACTGTTTTTCGCGGGAATTATGCTGGTTCCCGCATACTCCGCAAAAAGAAAACGCGGTCAAGAGAAGTGAGTCTCTGTTCCGGGTGTGTATGTTGATTATAATTTTccc                                                                                                      >  1:4178885/1‑140 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTTGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTccc                                                                                                      >  1:3875472/1‑140 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTccc                                                                                                      >  2:1668037/1‑140 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:3466303/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:1230287/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:3350173/140‑1 (MQ=255)
                                             tccTATGCGCTGGACTGGCATCCTACTGTTTTTCGCGGGACTTCTGATGGTTCCCGCATCCGCAGCACAAAGAAAACCCGGGAACGAGACGTGCGTCGCTGTTCGGGGGTGTTATGTTTATGGTAATTTTCCcgcgcggc                                                                                               >  2:2685974/2‑138 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:3739043/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:2140029/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGTTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:4642609/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATtct                                                                                                                                         <  2:100150/98‑1 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTGTGTTGTCCGGGTGTTTCTGTGTATGATAATTTTCCCGCGCGtc                                                                                               >  1:3557430/1‑139 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:1314725/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:1109545/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:4578911/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:898381/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:5186116/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:756376/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:2361607/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1944948/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:4758992/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTAGGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:2786151/1‑140 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTGTCGGGGTTTTTATGTTTATTATAATTTTCGCGCGCGGCTATc                                                                                           >  2:3937800/1‑140 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATATTTTTCCCGCGCGTATATc                                                                                           >  2:5014891/1‑140 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           >  1:3141353/1‑140 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGGGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATc                                                                                           <  1:1578720/140‑1 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:2394662/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  2:173802/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:2883400/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:3030675/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:5034291/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:4204747/1‑140 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTGTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTc                                                                                    >  2:4061031/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       >  2:3202731/1‑120 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:3202731/120‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGACAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:5133335/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:5152996/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAAATATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAACAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGGATATCGTCTGTCAtt                                                                                 <  2:330413/140‑1 (MQ=255)
                                                            tGGCATCATACTGTTTTGCGCGGGAATTATGATGGTTCCCTCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGCTGTctctta                                                                               <  1:815912/140‑6 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  1:2744716/1‑140 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  1:4012265/1‑140 (MQ=255)
                                                              gCATCAGACTGTTTGTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              <  2:3825516/140‑1 (MQ=255)
                                                              gCATCAGACTGTGTTTCGCGGGAATTCTGATCGTTCCCGCATACTCACCAACCACAAACCCCGCGAACGAGAAGTGCTGCTCTTTTCCGGGATAGTATGTGTATTAGAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              <  2:4029948/140‑1 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTCTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAACGAGACGTGATTGTCTTTGCCGGGTTTTTATGTTTATTATAAGTTTCCCGCGCGTATATCGTCTGTCATTCCTTCaga                                                                         >  2:3270830/1‑138 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  1:4699929/1‑140 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAACGAg                                                                    <  2:4563148/140‑1 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGGATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  1:2644999/1‑140 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGAAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCAGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  2:2003065/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                   >  1:864330/1‑140 (MQ=255)
                                                                            ttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           >  1:2234933/1‑113 (MQ=255)
                                                                            ttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           <  2:3410823/113‑1 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAACGAGAAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAGGAGCTTTCg                                                              >  1:4134874/1‑140 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTGTGTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAGGAGCTTTCg                                                              >  2:1152470/1‑140 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCCTAAGGAGCTTTCg                                                              >  2:2855660/1‑140 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCg                                                              >  2:1937424/1‑140 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTGTATTATAATTTTCCCGCGCGTATCTCGTCTGTCATTCCTTCATAAGGAGCTTTCg                                                              >  2:2938873/1‑140 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:598766/101‑1 (MQ=255)
                                                                                      ttATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTAct                                                      >  2:1534544/1‑140 (MQ=255)
                                                                                       tATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActt                                                     <  2:2361607/140‑1 (MQ=255)
                                                                                         tgatgGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTGTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActtct                                                   <  1:3764761/140‑1 (MQ=255)
                                                                                               ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGTTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  1:4901111/1‑140 (MQ=255)
                                                                                               ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  1:4373161/1‑140 (MQ=255)
                                                                                               ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTCTATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  2:591004/1‑140 (MQ=255)
                                                                                               ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  2:2545317/1‑140 (MQ=255)
                                                                                               ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGTTTATGATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  2:2754503/1‑140 (MQ=255)
                                                                                                 gccGCATACTCAGAAAAAATAAAACCCGGTAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           <  2:4256006/139‑1 (MQ=255)
                                                                                                   cGCATACTCAGCAAAAAGAAAACCCGGGAAAGTGACGTGTTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTGCCCGCGCGGATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGa                                         <  2:4974492/140‑1 (MQ=255)
                                                                                                         cTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCt                                   >  2:3932930/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGTTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:1275691/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:2613535/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:2299431/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:2078523/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:1094786/1‑140 (MQ=255)
                                                                                                             gCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAtt                               <  2:2253241/140‑1 (MQ=255)
                                                                                                             gCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAtt                               <  2:1379277/140‑1 (MQ=255)
                                                                                                                  aaGAACACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  1:3313692/140‑1 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  1:4828531/140‑1 (MQ=255)
                                                                                                                        aCCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACt                                       <  2:2375321/121‑1 (MQ=255)
                                                                                                                         cccGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGt                   <  2:17600/140‑1 (MQ=255)
                                                                                                                             ggAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAAtc               <  2:4202548/140‑1 (MQ=255)
                                                                                                                             ggAAAGAGAACTGACTCTCTTTTCCGGGTTTTTATCTATATTATAATTTACCCGCGCGTATATCGTCTGTCATTCCTTCATACGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAGTTTTCAGAAAGGTAAtc               <  1:1626669/140‑1 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:4604848/140‑1 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:1314725/140‑1 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAAtt     <  2:3563789/140‑1 (MQ=255)
                                                                                                                                        tGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc    <  1:1000923/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: