Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,375,849 T→G 6.7% N263H (AAC→CAC)  trxB ← thioredoxin‑disulfide reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,375,8490TG 6.7% 794.5 / 6.4 291N263H (AAC→CAC) trxBthioredoxin‑disulfide reductase
Reads supporting (aligned to +/- strand):  ref base T (89/172);  new base G (28/2);  total (117/174)
Fisher's exact test for biased strand distribution p-value = 1.86e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

AGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGCGTTACG  >  NZ_CP034943/3375713‑3375988
                                                                                                                                        |                                                                                                                                           
 gTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACGTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGACTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtttgtt                                                                                                                                         <  1:2845016/140‑1 (MQ=255)
  ttcttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtttgttt                                                                                                                                        <  1:154152/140‑1 (MQ=255)
    cttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtttgtttag                                                                                                                                      >  1:2946510/1‑138 (MQ=255)
       cGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATg                                                                                                                                   <  1:5030982/140‑1 (MQ=255)
       cGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATg                                                                                                                                   <  2:4179553/140‑1 (MQ=255)
       cGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATg                                                                                                                                   <  2:1375053/140‑1 (MQ=255)
            tAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGcc                                                                                                                              <  2:751278/140‑1 (MQ=255)
             aaTGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTAGCct                                                                                                                             >  1:928665/1‑140 (MQ=255)
                    aaCGCTTTGTGCCGCAATACTTCCGTCTCCTGTCGCCGTTACAAGTTGGCGCAGTGACTTGTCGCGAATGTCTCCGGCAGCGAAAATACCTTCATCCTTTGTTTCCATGCGGTCGTGTGTTTAGATGTAGCcttcttact                                                                                                                      >  1:1942377/1‑137 (MQ=255)
                    aaCGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCAt                                                                                                                      >  1:1175460/1‑140 (MQ=255)
                    aaCGCTTTGTGCCGCAATACTTCCGTCACCTCTAGCCGTTACAATTTGGCGCAGTGAGTTTTAGCGAATGTCTCCGGAATCTAAAAAACATTCAAACTTTATTTCCATGAGGTCGTTTGTTTCTATGTAGCATTATTAAt                                                                                                                      >  2:4005623/1‑140 (MQ=255)
                    aaCCCTTTGTGCCGCAATACTTCCGTCGCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTGGTTTCCATGCGGTCGTTTGTTTCGGCGTAGGCGTGTTCAt                                                                                                                      >  1:2019646/1‑140 (MQ=255)
                         tttGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTa                                                                                                                 <  2:547978/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCTGTAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:2967161/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:2343876/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:1290430/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:2850233/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:3157406/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:4082217/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:1413957/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:4737733/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:1121187/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:1179718/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:69786/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:2153386/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:2333612/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:2205039/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:3670101/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:4866472/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:3565660/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:3175721/140‑1 (MQ=255)
                                         tCCGTCTCCTGTAGACGTTAAAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCACGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  1:2494909/140‑1 (MQ=255)
                                         tCCCTCTCCTCCAGCCGTTACACTTTGGCGCAGTGACTTTTCGCGAAGGGCTCCGGCAGCGAAAATACCTCCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                                 <  2:2695134/140‑1 (MQ=255)
                                          ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAATTACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGGTGTTTGCGTCGTCATTCGTAATGCCCAGATTTTGGaa                                                                                                >  1:3396789/1‑140 (MQ=255)
                                          ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTGGTTGTAGCCGTCTTCATTGGTAATGGCCCGAttttggga                                                                                                >  1:447081/1‑136 (MQ=255)
                                          ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGGGTGTTTCGATGTAGCCTTCTTCATTCGTCATGCCCAGATTTTCGaa                                                                                                >  2:1598332/1‑140 (MQ=255)
                                          ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTGGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGaa                                                                                                >  2:3687465/1‑140 (MQ=255)
                                           cGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAAc                                                                                               <  2:2227343/140‑1 (MQ=255)
                                           cGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAAc                                                                                               <  2:1772149/140‑1 (MQ=255)
                                           cGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAAc                                                                                               <  2:1325266/140‑1 (MQ=255)
                                             tctcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTGGATGTAGCCTTCTTCATTGGTAATGACCCGATTTTCGAACgg                                                                                             >  1:3089509/1‑140 (MQ=255)
                                             tctcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTAGATGTAGGCGTGGTCATTGGGAATTGCCCGATTTTGGGACgg                                                                                             >  1:2666011/1‑140 (MQ=255)
                                             tctcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTAGATGTAGCCTTAGTGATTGGTAATTGCCCGATTTTCGAAAgg                                                                                             >  1:4440589/1‑140 (MQ=255)
                                              ctcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTAGCCGTCTTCAATCGTAAAGGCCAGATTTTCGAACggg                                                                                            >  1:1002895/1‑139 (MQ=255)
                                               tcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGtt                                                                                           >  2:2161638/1‑140 (MQ=255)
                                                 cTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTa                                                                                         <  2:2429269/140‑1 (MQ=255)
                                                  tGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATTCGGTCGTTTGTTTCGATGTAGCCTTATTCATTAGTAATGCCCAGATTTTCGAAAGGGTTAg                                                                                        >  2:798520/1‑140 (MQ=255)
                                                  tGTAGCCGTTACAATTTGGCGCAGTGACTTGTCGCGAATGTCGCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAg                                                                                        <  1:4361754/140‑1 (MQ=255)
                                                   gTAGCCGTTACAATTTGGCGCAGTGACTGTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                       <  1:3259751/140‑1 (MQ=255)
                                                    tAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATTTCTCCGGCAGCGAAAATACCTTCAACATTTGTTTCCATGCGGTCGTTTGTTTAGATGTAAACATCTTCAATAGTAAAGCCCAGATTTTAGAAAGGTTTAGAc                                                                                      >  2:1007539/1‑140 (MQ=255)
                                                    tAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTAGATGTAGCCTTCTTCATTCGTAAGGCCCAGATTTTCGAACGGTTTAGAc                                                                                      >  2:2096984/1‑140 (MQ=255)
                                                      gCCGTTACAATTTGGCTGAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACag                                                                                    <  1:4005623/140‑1 (MQ=255)
                                                      gCCGTTACAAGTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTACAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGGCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACag                                                                                    <  2:2596550/140‑1 (MQ=255)
                                                         gTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCcttc                                                                                                                           <  1:387046/98‑1 (MQ=255)
                                                           tACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCCGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTAGTTTTAGCCTTCTTCATTCGGCATGCCGCGATTTTAGAACGGTTTAGAAAGTggga                                                                               >  1:1309498/1‑138 (MQ=255)
                                                           tACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTTTTTCGATGTAGCCTTATTCATTGGTAATGCCCACATTTTCGAACGGTTTATACACAGGaa                                                                               >  1:4778751/1‑140 (MQ=255)
                                                           tACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGAAAGATGaa                                                                               >  2:1821436/1‑140 (MQ=255)
                                                           tACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTAGATGTATCCTTCTTAATTAGTAATGCCCAGATTTTCGAACGGGTTATACAAAGGaa                                                                               >  2:4400140/1‑140 (MQ=255)
                                                            acgaTTTGGCGCAGTGATTTTTCGCGGATGTCTCCGGCAGCGAAAATCCCTTCAACCTTAGTTTCCATGCGGTCGTTTGTTTCGATATAGCCTTCTTCATTCGTAATGCCCAGGTTTTCAAACGGTTTAGACAGAGGAAg                                                                              >  1:3683520/4‑140 (MQ=255)
                                                                 ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                         <  1:137423/140‑1 (MQ=255)
                                                                 ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                         <  1:4894702/140‑1 (MQ=255)
                                                                 ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                         <  1:303969/140‑1 (MQ=255)
                                                                 ttGGCGCAGTGACTTTTCGCGAAGGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                         <  2:4671644/140‑1 (MQ=255)
                                                                 gtGGCGCTGGGACATGTCGCGCAGGTCTCCGGCAGCGAAAATAGCTTCAACCTGTGGTTCCATGCGGTCGTGTGTTGCGATGTAGCCTTCTTCATTCGTAATGCCCAGATGTTCGAACGGTTTTGACAGTGGAAGCATGc                                                                         <  1:3616399/139‑1 (MQ=255)
                                                                    gcgcAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCCATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGAACGAGGCAGCATGCCGa                                                                      >  2:630987/1‑140 (MQ=255)
                                                                        aGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTa                                                                  <  1:2296277/140‑1 (MQ=255)
                                                                        aGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTa                                                                  <  1:1625867/140‑1 (MQ=255)
                                                                          tGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCATTGTTTCCATGCGGTCGTGTGTTTCGATGTTGCCTTCTACATTCGTTATTCCCAGATATTAGAACGGGTTTTGCATAGGGCGCAAGCCGTTGTaaa                                                                >  1:5168382/1‑140 (MQ=255)
                                                                               tttCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTAATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCAGTCCGATGTAAATTAAt                                                           >  2:2980758/1‑140 (MQ=255)
                                                                                ttCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCCTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTAGAACGGTTTAGACAGAGGAAGCATGCCGCTGTAAATGaaaa                                                          >  2:2259364/1‑138 (MQ=255)
                                                                                ttCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGAAGTATCCATAGTCATTGGTAATTCCCAGATTTTCGAACGGTTTTGACAGAGGAAGCCTGCCGATGTAAATGAATa                                                          >  2:391486/1‑140 (MQ=255)
                                                                                     gAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCg                                                     <  2:2659016/140‑1 (MQ=255)
                                                                                      aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGGTGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                    <  2:1147112/140‑1 (MQ=255)
                                                                                      aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                    <  1:4585430/140‑1 (MQ=255)
                                                                                      aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                    <  1:3801900/140‑1 (MQ=255)
                                                                                                    cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTGGATGTAGCCTTCGTCATTCGTAATGCCCCGATTTTGGAACGGTTTAGACAGAGGCAGCATGCCCATGTAAATGAATACGCCGTCTGTTTTGAATTCa                                      >  2:4795445/1‑140 (MQ=255)
                                                                                                    cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCa                                      <  2:3770056/140‑1 (MQ=255)
                                                                                                    cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTATTCATTCGTAATGGCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGGCGGCTGTTTTTTATTCa                                      >  2:2867346/1‑140 (MQ=255)
                                                                                                    cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGGTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGGAGCATGCCGATGTAAATTAATACGCCGTCTGTTTTGAATTcc                                      >  2:2773342/1‑139 (MQ=255)
                                                                                                    cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTGGTTTTAGCCGTTTTGATTCGTAATGCCCCGATTTTGGGACCGTTTAGACAGAGGAAGCCTGGCGATGTAAATGAATAAGCCCTGTGtttttgattga                                      >  1:4948561/1‑133 (MQ=255)
                                                                                                      aaaaTACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACt                                    <  2:3848961/140‑1 (MQ=255)
                                                                                                      aaaaTACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACt                                    <  2:5065972/140‑1 (MQ=255)
                                                                                                             cTTCATCGTTGGTTTGGATGCGGTCGTTTGTTTGGATTTATGGTTCTTGTTTCGTAATGCCCAGATTTTCGAAAGTATTAGAGAGAGGAAGCATGCCGATTTAAATGAATATGCCGTGTGTTTTGAATTCAGTTTCTTCa                             <  2:5166884/140‑1 (MQ=255)
                                                                                                             cTTCAACCTTTGTTTCCCTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTATTGCCCAGTTTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGATTACGCCGTCTGTTTTGAATTCACTTTCTTCa                             <  2:3820020/140‑1 (MQ=255)
                                                                                                                cAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTAGCCATCTTAAATCGTAATGCCCAGATTTTAGAACGGTTTAGACCCAGGAACCAAGCCGATTTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTAACCg                          >  2:3267611/1‑140 (MQ=255)
                                                                                                                  aCCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGt                        <  1:3774676/140‑1 (MQ=255)
                                                                                                                  aCCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGAGGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGt                        <  1:3933721/140‑1 (MQ=255)
                                                                                                                    cTTTGTTTGCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTATTGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCCCTTTCTTCACCGGtga                      <  2:4983650/140‑3 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACag                                                                                    <  1:2752957/68‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc            <  1:1495933/140‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc            <  1:535451/140‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc            <  1:1766889/140‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc            <  2:4117380/140‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc            <  1:2592457/140‑1 (MQ=255)
                                                                                                                              aTGCGGTCGTTTGTTTCGATGTAGCCATCTTCATTCGTAATGCCCCGATTTTCGAACGGTTTTGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTGGGTTTTGAATTCACGTTATTCACCGGTTACCGTATAGAc            >  2:3483233/1‑140 (MQ=255)
                                                                                                                                 cGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAg         <  1:4291370/140‑1 (MQ=255)
                                                                                                                                   gTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGcg       <  2:447081/140‑1 (MQ=255)
                                                                                                                                   gTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGcg       <  2:3396789/140‑1 (MQ=255)
                                                                                                                                      gtttgtttCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGcgtta    <  2:67262/140‑1 (MQ=255)
                                                                                                                                        ttgtttCGATGTAGCCTTATTCATTCGTAATGCCCAGATTTTGGAACGGTTTATACAGAGGAAGCATGCCGATGTAAATTAAAACCCCGTCTGTTTTGAATTCAATTTCTTAACCGGTTAACGTAACGACAAGcgttacg  >  2:1956392/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
AGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGCGTTACG  >  NZ_CP034943/3375713‑3375988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: