Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,962 C→A 12.2% M208I (ATG→ATT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9620CA12.2% 663.3 / 16.1 332M208I (ATG→ATTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (107/160);  new base A (56/2);  total (170/162)
Fisher's exact test for biased strand distribution p-value = 5.52e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

TTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATG  >  NZ_CP034943/3446823‑3447101
                                                                                                                                           |                                                                                                                                           
ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAcattc                                                                                                                                             >  2:4193884/1‑140 (MQ=255)
 tataACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAcagtca                                                                                                                                            >  1:3763230/1‑140 (MQ=255)
        ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCCTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTGGATTCAGTGTTTg                                                                                                                                     >  1:1191175/1‑140 (MQ=255)
        ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                     <  2:3827284/140‑1 (MQ=255)
         tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAtttttttc                                                                                                                                    >  2:2758678/1‑138 (MQ=255)
         tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCAtttttttc                                                                                                                                    >  2:2494405/1‑138 (MQ=255)
         tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCACAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGTCTTGCGTCCGGTCGTTTAAATTAATTTTTTga                                                                                                                                    >  2:1455525/1‑139 (MQ=255)
          aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                   <  1:1802237/140‑1 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaa                                                                                                                                  <  1:3003751/140‑1 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCaa                                                                                                                                  >  2:4985053/1‑140 (MQ=255)
                aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                             >  1:4983098/1‑140 (MQ=255)
                    aaaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCt                                                                                                                         <  2:150951/140‑1 (MQ=255)
                     aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                        >  1:4836435/1‑140 (MQ=255)
                      aCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                   >  2:4051877/1‑128 (MQ=255)
                             ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAATATTGCTCACATGGtt                                                                                                                >  2:3258097/1‑140 (MQ=255)
                                   aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                          <  1:4606283/140‑1 (MQ=255)
                                   aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCGACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                          <  1:4418244/140‑1 (MQ=255)
                                    aaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACg                                                                                                         <  1:1475114/140‑1 (MQ=255)
                                    aaaGACCTGCCCAGTAAAAGGCAAGTCTATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACg                                                                                                         <  2:3742816/140‑1 (MQ=255)
                                     aaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACgg                                                                                                        <  2:785661/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCCCACCGGCTTGCGTCCGGTCGTTTACAGTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                  <  2:693948/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                  <  1:547967/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                  <  1:3833281/140‑1 (MQ=255)
                                            gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTtgtc                                                                                                 >  2:1318477/1‑140 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCCGCCATTTTTAATGGCGACCACACCGGCTGGCGTCCGGTCGTTTTCATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                <  2:3121951/140‑1 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                <  1:3227165/140‑1 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                <  1:2216089/140‑1 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                <  2:4382330/140‑1 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTGAAAATATTGCTCACATGGTTTTTCACGGttttgtaa                                                                                                >  1:1730595/1‑136 (MQ=255)
                                             cccACTAAAAGGCAAGTCTCTTATTATCCTATCTCAGTTCTCCCCAGCCAGTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                <  2:4444268/140‑1 (MQ=255)
                                                   aaaacccAAGTCTCTTATTATACTATCTCATTTCTACCCACCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                          <  1:3035756/134‑1 (MQ=255)
                                                           agacactTATTATAAGATCTCATTTCTACCCCCCCATTTTTAAAGCCGACCACAACCGATTGCGTCCGGTCGTTTAAATTCATTTTTTTAAAAATATTCCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                  <  2:2348559/135‑1 (MQ=255)
                                                           aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                  <  2:1120812/140‑1 (MQ=255)
                                                           aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                  <  2:4548841/140‑1 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTCACGGGTTTATAACTTATAAACAATGAt                                                                                 >  1:1234588/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTGCAAAAAAATGCTCACATGGTTTTTCAAGGTTTTATAACTGATAAACaaataa                                                                                 >  1:2468203/1‑136 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTGAATTTTTTCAAAATATTGCGCAAAAGGTTTTTAAACGTTTTATAAAAGATAAAAAAAGAt                                                                                 >  1:2319451/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATTGTTTTTAACGGTTTTGTCACTGATaaaaaagtat                                                                                 >  1:4053747/1‑136 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAAGAt                                                                                 >  2:5171987/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTAAATTATAAAAAATGAt                                                                                 >  2:263372/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAAAATTTCTCAAATGGTTTTTAACGGTTTTGTGAATGATAAACAAAGAt                                                                                 >  1:1450240/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCAATTTTTGCAAAATAATGCGCACAACGTTTTTAACCGTTTTATAACAGATAAACAAAGAt                                                                                 >  1:1285190/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAACTTGATTTTTTGCAAAATATTGATAACATGGTTTTTAACGGTTTTATAACATATaaaaaaagtt                                                                                 >  1:2752915/1‑136 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAACTTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATGAt                                                                                 >  1:598107/1‑140 (MQ=255)
                                                             tctcTTATTATACTATCTCATTTCTCCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTACGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                <  2:738471/140‑1 (MQ=255)
                                                             tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                <  1:969783/140‑1 (MQ=255)
                                                              ctctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCAAATTGTTTTTAACGGTTTTCTCACTTATAAACAATTATTc                                                                               >  2:5053603/1‑140 (MQ=255)
                                                              ctctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATGAtta                                                                               >  2:800354/1‑139 (MQ=255)
                                                                 ttattaTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAcc                                                                            <  1:3123511/140‑1 (MQ=255)
                                                                   attatACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTTCAAAATATTGCTCACATTGTTTTTCACCGTTTTATCCATTATAAAAAATTATTAAACaa                                                                          >  2:2067348/1‑140 (MQ=255)
                                                                       taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTAACAAGGTTTTTCAAGGTTTTGTCACTGATAAACAAAGATTCACCAATAcc                                                                      >  2:3449254/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTAACATGGTTTTTAACGGTTTTCTAACTGATAAACAATGATTCACCAATAACGCgg                                                                  >  2:91816/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGATCACATGTGTTTTAACGGTTTTCTAACTGATAAACAATCATTAAACAATAACGCgg                                                                  >  2:3212430/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAAATTCTCACATGGTTTTTAACGTTTTTATCACTGATAAACAAAGATTCAACAATaaccccg                                                                  >  2:3098319/1‑134 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAAAAATTTTTTGCAAAAAATTTATCACAAGGTTTTTAAACGTTTTTTCACAGATAAACAAATATTAACCAAAAAcgccg                                                                  >  1:920854/1‑138 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCCAAAAAATGCTAACATGGTTTTTAACCCTTTTCTCACAGATAAAAAAAGATTCACCACTACAGCgg                                                                  >  1:1965143/1‑140 (MQ=255)
                                                                           aTCGCATTTCTAACCAGCCATTTTTAATGGCGACCACACCGGCTTGCGTCAGGTCGAGTAAATTAATTTTTTGCAAAAAATTTGTCACATGGTGTTTCACCGTTTTCTCACGGCTTAACAAAGATTAACCCATACCGCgg                                                                  >  2:4706628/1‑140 (MQ=255)
                                                                            tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTTTAAAATATTTCTTACATGGTTTTTAAAGGTTTTATCAATTATAAAAAAAGATTAAACAAAAACCCGGt                                                                 >  1:2838897/1‑140 (MQ=255)
                                                                              tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTg                                                               <  2:1487323/140‑1 (MQ=255)
                                                                               cATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATGATTCAACAATAACGCGGtttc                                                              >  2:4598034/1‑138 (MQ=255)
                                                                                 tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCCAAAAATTTCGCACATGGTTTTTAACGGTTTTATAACAGATAAACAAAGATTCACCCAAAACCCGGTTGcgt                                                            >  1:1014600/1‑138 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCTTCAATTTTTTCAAAATAATTCGCACAATGTTTGTGACGGTTTTCTCACTTATAAAAAATGATTAACCAATACCGCGGTTGCtttttac                                                       >  1:2546485/1‑138 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCCAAATATTGCTAACATGGTTTTTCACGGTTTTATCACTGATAAACAAAGTATCACCAAAACCCCGGTTGCattttgc                                                       >  2:623800/1‑138 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTcc                                                       <  2:3619087/140‑1 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTGAAAATATTTGTCACATGGTTTTTCACGGGTTTATCACTGATAAACAATGATTGCCCAAAACCCCGGTTGCtttttgc                                                       >  1:2091712/1‑138 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCAAGGTTTTATCACTGTTAAAAAATTATTAAACAATAACGCCGTTGCtttttac                                                       >  2:2951668/1‑138 (MQ=255)
                                                                                      aCCCAGCCATTTTTAATGGCGACCACAACGGCATGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGTTTTTCTCACTTATAAACAATTATTCACCAAAACAGCGGTTGCtttttac                                                       >  2:3870176/1‑138 (MQ=255)
                                                                                          aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTCACATGGTTTTTCAAGGTTTTCTCAATTATAAACAATGATTAAACAATACCGCGGTTGGTTTTTGCGGCt                                                   >  1:3577061/1‑140 (MQ=255)
                                                                                           gCCATTTTTAATGGCGAACACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTg                                                  <  2:964359/140‑1 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCAAATTGTTTTTAAAGGTTTTGTCACTGATAAAAAATGATTCACCAATAAAGCGGTTTCTTTTTACGTCTGc                                                 >  2:1735419/1‑140 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTGACCAATAACGCGGTTGCTTTTTCCGTCTgg                                                 >  2:3994444/1‑139 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATGATTTTTTGCAAAAAATTGCTCCCATGGTTTTTAACCGTTTTTTCACTGGTAAAAAAACATTCACCAAAAACCCGGTTGCTTTTTGCGTCAGc                                                 >  1:2935653/1‑140 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCAATTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTAACTGATAAACAAAGATTCACCAAAACAGCGGTTGCTTTTTCAGTCTGc                                                 >  2:1045322/1‑140 (MQ=255)
                                                                                            ccATTTTTAAAGGCGACCACAACGGCTTGCGTCCGGTCCTTTACCATCATTATTTTCGAAATATTGCTAAAATGCTTTTTCACGGTTTTATAAATGATAAACAATGATTAACAAATAACCAGGTTGGAGTTTCCGTCTga                                                 >  2:4021729/1‑139 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                <  1:3449254/140‑1 (MQ=255)
                                                                                             cATTTTTAAGGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                <  2:3567830/140‑1 (MQ=255)
                                                                                               tttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTAAATTTTTTCAAAAAATTTGTCCCCTGGTTTTTGACCGTTTTATCACTTATAAAAAAAGATTAACCAAAACCCCCGTTGGTTTTTACGtggtcaac                                              >  1:119993/1‑133 (MQ=255)
                                                                                                ttttAATGGCGACCCCCACGCCTTGCCTCCGGTCGTTTACATTCATTTTTGGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCCCGGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                             <  2:4104469/140‑1 (MQ=255)
                                                                                                ttttAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                             <  2:1617310/140‑1 (MQ=255)
                                                                                                ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                             <  1:4193884/140‑1 (MQ=255)
                                                                                                ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                             <  2:421855/140‑1 (MQ=255)
                                                                                                     aTGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                        <  2:2973986/140‑1 (MQ=255)
                                                                                                        gCGACCACAACGGATTGCGTCCGGTCGTTTACATTCATTTTTAGCAAAATATTGCTAACATGGTTTTTCACGGTTTTCTCAATGATAAACAATGATTCACCAATACCGCGCTTGCTTTTTCCGTCTGCAAGCATCTgcat                                     <  1:4373962/140‑2 (MQ=255)
                                                                                                          gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                   <  1:5041763/140‑1 (MQ=255)
                                                                                                          gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTgcagca                                   <  1:2067348/140‑1 (MQ=255)
                                                                                                          gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                                   <  1:4888865/140‑1 (MQ=255)
                                                                                                          gACCACAACGGCTTGCGTCCGGTCGTGTACCTTCATTTTTTTCAAAATATTGCACACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTTCAAGCATCTgcagca                                   >  2:1189084/1‑140 (MQ=255)
                                                                                                           aCCACAACGGCTTGCGTCCGGTCGTGTTCAATCATTTTTTTCAAAATAATTCTCACATGGTGTTTGACGGTTTTATCTAGTCTAAAAAATTATTGAACAAAAACCCGGTTGGTTTTTCAGGGTGGAAGCATCGGCAGCAc                                  >  1:4655005/1‑140 (MQ=255)
                                                                                                             cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                <  1:3246778/140‑1 (MQ=255)
                                                                                                                  cGGCTTGCGTCCGGTCGTTTTCATTAATTTTTTTGAAAATATTTCTCACATTGTTTTTGACGGTTTTGTAACAGATAAAAAATGATTCACCAAAACCGCGGTTGGTTTTTGCGTCTGCAAGCATATGCCGCCCGTcacaa                           >  1:4021187/1‑139 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                      <  2:1849072/140‑1 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                      <  2:199686/140‑1 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                      <  2:5091170/140‑1 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                      <  2:2237090/140‑1 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTTGTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTaccc                      >  2:1957823/1‑139 (MQ=255)
                                                                                                                          gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATTGTTTTTCACGGTTTTATAACTTATAAAAAATGATTCAACAAAAACCCGGTTTCTTTTTCCGTCTGCAAGCATCTGCAGCACTTAACATTCccgccg                   >  2:3776788/1‑140 (MQ=255)
                                                                                                                          gTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTGCGGGTGCAAGCAGATGGAGCCCGTGACATTCccgccg                   >  2:1807868/1‑140 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa            <  1:1589787/140‑1 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa            <  2:3312480/140‑1 (MQ=255)
                                                                                                                                        attcattTTTTGCACAAGATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa     <  1:4269651/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATG  >  NZ_CP034943/3446823‑3447101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: