Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,980 G→T 14.7% S202R (AGC→AGA degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9800GT14.7% 745.8 / 36.6 342S202R (AGC→AGAdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (93/186);  new base T (60/2);  total (153/189)
Fisher's exact test for biased strand distribution p-value = 4.04e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

AAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446843‑3447119
                                                                                                                                          |                                                                                                                                           
aaaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCt                                                                                                                                           <  2:150951/140‑1 (MQ=255)
 aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTCTACGATCTCATTTCTACCCAGCCATTTTTAATGGCGACCCCAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAATAGTGGTc                                                                                                                                          >  2:3181678/1‑140 (MQ=255)
 aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTCTCTCATTTCTAGCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTGGAAAATATTGCTc                                                                                                                                          >  2:2195363/1‑140 (MQ=255)
 aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                                          >  1:4836435/1‑140 (MQ=255)
        gCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGt                                                                                                                                   <  2:194901/140‑1 (MQ=255)
         ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAATATTGCTCACATGGtt                                                                                                                                  >  2:3258097/1‑140 (MQ=255)
               aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                            <  1:4606283/140‑1 (MQ=255)
               aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                            <  1:1787969/140‑1 (MQ=255)
                 aaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACgg                                                                                                                          <  2:785661/140‑1 (MQ=255)
                       tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                    <  1:1213344/140‑1 (MQ=255)
                       tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                    <  1:3833281/140‑1 (MQ=255)
                        gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTGACATTGTTTTTAACGGTTTtctc                                                                                                                   >  2:1798834/1‑140 (MQ=255)
                        gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtctc                                                                                                                   >  2:5008631/1‑140 (MQ=255)
                        gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTtctc                                                                                                                   >  2:2965409/1‑140 (MQ=255)
                         cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  1:2216089/140‑1 (MQ=255)
                         cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  2:4382330/140‑1 (MQ=255)
                         cccACTAAAAGGCAAGTCTCTTATTATCCTATCTCAGTTCTCCCCAGCCAGTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  2:4444268/140‑1 (MQ=255)
                             gTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGa                                                                                                              <  2:3078283/140‑1 (MQ=255)
                             gTAAAAGGCAAGTATATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGa                                                                                                              <  2:4331239/140‑1 (MQ=255)
                               aaaacccAAGTCTCTTATTATACTATCTCATTTCTACCCACCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                            <  1:3035756/134‑1 (MQ=255)
                               aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                            <  1:1433236/140‑1 (MQ=255)
                                   ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATCAATTTTTTCAAAAAATTGCTAACATGGTTTTTAACGGTTTTATAACTTATaaaaa                                                                                                        >  1:4036427/1‑138 (MQ=255)
                                   ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                                        <  2:3276529/140‑1 (MQ=255)
                                     cAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAt                                                                                                      <  2:374341/140‑1 (MQ=255)
                                       aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                    <  2:658873/140‑1 (MQ=255)
                                       aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                    <  2:4548841/140‑1 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAAATAAATTTTTTGAAAAAATTTGTAACAATGTTTTTCACAGTTTTTTGACTTAAAAAAAATTAt                                                                                                   >  1:734897/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTATCACTGATAAACCATGAt                                                                                                   >  1:5130479/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGATCACATTGTTTTTAAACGTTTTGTAACAGATAAAAAAAGAt                                                                                                   >  1:1721293/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAAGAt                                                                                                   >  2:5171987/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTAAATTATAAAAAATGAt                                                                                                   >  2:263372/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTTCTCAAATTGTTTTTCAACGTTTTATAACTGATAAAAAATTAt                                                                                                   >  1:3692750/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTAAAAATATTTATAACAAGGTTTTTAACGGTTTTCTCACTTATaaaaaaatat                                                                                                   >  1:300655/1‑136 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAAAATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                   >  1:806342/1‑140 (MQ=255)
                                        gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTGCAAAATATTTCTCACATGGTTTTTCACCGTTTTATCACTGATAAACaaatat                                                                                                   >  1:2357922/1‑136 (MQ=255)
                                         tctcTTATTATACTATCTCATTTCTCCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTACGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                  <  2:738471/140‑1 (MQ=255)
                                         tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                  <  1:3573131/140‑1 (MQ=255)
                                         tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                  <  2:283299/140‑1 (MQ=255)
                                             ttattaTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAcc                                                                                              <  1:3123511/140‑1 (MQ=255)
                                                   taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTAACAAGGTTTTTCAAGGTTTTGTCACTGATAAACAAAGATTCACCAATAcc                                                                                        >  2:3449254/1‑140 (MQ=255)
                                                       aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACAAGGTTTTTAAAGGTTTTCTAACAGATAAACAAAGATTCACCAATAAAGCgg                                                                                    >  2:3123511/1‑140 (MQ=255)
                                                       aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAAAATTGCTCACAAGGTTTTTAACGGTTTTCTCACAGATAAAAAAAGATTTACCAATAAcgccg                                                                                    >  1:1895483/1‑138 (MQ=255)
                                                       aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAAATTTTTTAAAAAAATTTCTAACATGGTTTTTAAACGTTTTATCACTTATAAACAATGATTCACCAAAAACGCgg                                                                                    >  1:2930638/1‑140 (MQ=255)
                                                       aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATAATTTTTTTGAAAATATTTGTCACAAGGTTTTTAACCGGTTTGTCACAGAAAAAAAAAGATTAACCAAAAACGCgg                                                                                    >  1:125365/1‑140 (MQ=255)
                                                       aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTAATTTTTTTCAAAAAATTTCTAACAAGGTTTTTAAAGGTTTTGTCACAGATTAACAAAGATTCACCAAAAcatcgg                                                                                    >  1:3375905/1‑135 (MQ=255)
                                                        tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAAATTCTCACAATGTTTTTCACGGTTTTATCACTGATAAAAAAAGATTAACCAAAAACGCGGt                                                                                   >  1:4997627/1‑140 (MQ=255)
                                                        tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTTTAAAATATTTCTTACATGGTTTTTAAAGGTTTTATCAATTATAAAAAAAGATTAAACAAAAACCCGGt                                                                                   >  1:2838897/1‑140 (MQ=255)
                                                          tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTg                                                                                 <  2:1487323/140‑1 (MQ=255)
                                                          tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTg                                                                                 >  2:3806207/1‑140 (MQ=255)
                                                           cATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATGATTCAACAATAACGCGGtttc                                                                                >  2:4598034/1‑138 (MQ=255)
                                                             tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATAATTCTCACAATGTTTTTAACGGTTTTATAACTGATAAACAAAGATTAAACAAAAACGCGGGTGGtt                                                                              >  1:4142224/1‑140 (MQ=255)
                                                                  aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCTTCAATTTTTTCAAAATAATTCGCACAATGTTTGTGACGGTTTTCTCACTTATAAAAAATGATTAACCAATACCGCGGTTGCtttttac                                                                         >  1:2546485/1‑138 (MQ=255)
                                                                  aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTcc                                                                         <  2:3619087/140‑1 (MQ=255)
                                                                  aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTAAAGGTTTTGTCACTGATAAAAAATTATTAACCAAAACCGCGGTTTCtttttac                                                                         >  2:1630634/1‑138 (MQ=255)
                                                                  aCCCAGCCATTTTTAATGGCGACCACAACGGCATGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGTTTTTCTCACTTATAAACAATTATTCACCAAAACAGCGGTTGCtttttac                                                                         >  2:3870176/1‑138 (MQ=255)
                                                                      aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTAAAAATATTTCGAACAAGGTTTTTCAAGGTTTTGTAACTGGTAAACAATGATTCAGCAATACCGCGGTTGCTTTTTCCGTCt                                                                     >  2:4195389/1‑140 (MQ=255)
                                                                       gCCATTTTTAATGGCGAACACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTg                                                                    <  2:964359/140‑1 (MQ=255)
                                                                        ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCAAATTGTTTTTAAAGGTTTTGTCACTGATAAAAAATGATTCACCAATAAAGCGGTTTCTTTTTACGTCTGc                                                                   >  2:1735419/1‑140 (MQ=255)
                                                                        ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGc                                                                   >  2:1349277/1‑140 (MQ=255)
                                                                        ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCAATTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTAACTGATAAACAAAGATTCACCAAAACAGCGGTTGCTTTTTCAGTCTGc                                                                   >  2:1045322/1‑140 (MQ=255)
                                                                        ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCCTTTTTTGGAAAATATTTCTCACATGGTTTTTAACGGGTTTGTCACAGATAAACCAATATTCACCCATACCGCGGTTGCTTTTTGCGTCTGc                                                                   >  1:2705944/1‑140 (MQ=255)
                                                                         cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                  <  1:2085087/140‑1 (MQ=255)
                                                                         cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCa                                                                  <  2:2088626/140‑1 (MQ=255)
                                                                         cATTTTTAAGGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                  <  2:3567830/140‑1 (MQ=255)
                                                                            ttttAATGGCGACCCCCACGCCTTGCCTCCGGTCGTTTACATTCATTTTTGGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCCCGGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  2:4104469/140‑1 (MQ=255)
                                                                            ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  2:119993/140‑1 (MQ=255)
                                                                            ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  1:1196030/140‑1 (MQ=255)
                                                                                    gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  1:3212593/140‑1 (MQ=255)
                                                                                    gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  2:4036427/140‑1 (MQ=255)
                                                                                      gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                  <  1:964447/95‑1 (MQ=255)
                                                                                      gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                     <  1:146557/140‑1 (MQ=255)
                                                                                      gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                     <  2:1146821/140‑1 (MQ=255)
                                                                                      gACCACAACGGCTTGCGTCCGGTCGTTTACAGTCATTTTTTGGAAAATATTGGTCCCATGGTTTTTCACGGTGTTGTCACTGATAAACACTGATTCACCAATACCGCGGTTGGTTTTTGCGTCTGCAAGCATCGtcagcc                                                     >  1:4239534/1‑139 (MQ=255)
                                                                                       aCCACAACGGCTTGCGTCCGGTCGTGTTCAATCATTTTTTTCAAAATAATTCTCACATGGTGTTTGACGGTTTTATCTAGTCTAAAAAATTATTGAACAAAAACCCGGTTGGTTTTTCAGGGTGGAAGCATCGGCAGCAc                                                    >  1:4655005/1‑140 (MQ=255)
                                                                                         cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                  <  1:3246778/140‑1 (MQ=255)
                                                                                         cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                  <  1:593702/140‑1 (MQ=255)
                                                                                         cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                  <  1:4324157/140‑1 (MQ=255)
                                                                                           aaaaggCGTGCGTCCGGTCGTTTACATTACTTTTTTGCAAAATATTGCTCAAATTGTTTTTCACGGTTTTCTCAATGATAAAAAATGATTCACCCATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                <  1:5047295/136‑1 (MQ=255)
                                                                                              cGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTGTCACTGATAAACAATGATTCACCAAAACCCCGGTTGCGTTTTCCGGGTGCAAGCAACTGCAGCACGTCACAt                                             >  2:80965/1‑140 (MQ=255)
                                                                                              cGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAAAATTTCTCACAAGGTTTTTGACGGTTTTGTCACTGATAAAAAAATATTCACCAAAAACCCGGTTGGTTTTTGCGTCTGCAATCAACTGCAGCAcataaaaa                                             >  1:1981489/1‑133 (MQ=255)
                                                                                                   tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                        <  2:199686/140‑1 (MQ=255)
                                                                                                   tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                        <  2:1849072/140‑1 (MQ=255)
                                                                                                   tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                        <  2:5091170/140‑1 (MQ=255)
                                                                                                     cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAAGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                      <  2:2347676/140‑1 (MQ=255)
                                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCTCTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                            <  1:1547267/140‑1 (MQ=255)
                                                                                                                     attctttTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                       <  1:4391275/140‑1 (MQ=255)
                                                                                                                        cattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATg                    <  2:4021187/140‑1 (MQ=255)
                                                                                                                         attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                   >  2:914471/1‑140 (MQ=255)
                                                                                                                         attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                   >  2:4740961/1‑140 (MQ=255)
                                                                                                                           tttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTc                 >  1:4476034/1‑140 (MQ=255)
                                                                                                                           tttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTc                 >  1:2281080/1‑140 (MQ=255)
                                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:3918295/140‑1 (MQ=255)
                                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  1:2494405/140‑1 (MQ=255)
                                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:3355016/140‑1 (MQ=255)
                                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:1036082/140‑1 (MQ=255)
                                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:1006488/140‑1 (MQ=255)
                                                                                                                                          gCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:2468203/140‑1 (MQ=255)
                                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:300655/140‑1 (MQ=255)
                                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:598107/140‑1 (MQ=255)
                                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:4979739/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446843‑3447119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: