Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,498,775 C→A 8.0% Q2K (CAA→AAA)  EO946_RS18005 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,498,7750CA 8.0% 541.4 / 3.1 254Q2K (CAA→AAA) EO946_RS18005spore germination protein
Reads supporting (aligned to +/- strand):  ref base C (74/148);  new base A (6/26);  total (80/174)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

AAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACA  >  NZ_CP034943/3498639‑3498912
                                                                                                                                        |                                                                                                                                         
  aaCGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATc                                                                                                                                      >  1:3840596/1‑140 (MQ=255)
   aCGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATACAGGGGAGAGAAACTATGCAAATCg                                                                                                                                     >  1:4301633/1‑140 (MQ=255)
   aCGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCg                                                                                                                                     >  2:626126/1‑140 (MQ=255)
    cGCTTCCTATCTATTCTATCACTTTCAATATAAGGTTTGTTTAAGCCTTTTCCTTATTAATAATAATTCGCCTATTTTGATACAAATTAATAAAAACCGCCAATCTGTTTTATAAAGGGGAGAGAAAATATGCAAATCGa                                                                                                                                    <  2:952967/140‑1 (MQ=255)
         ccTTTTTTTTAGACCACTTTCATTATACTGTTTGTTTCACCCTTTTCCTTAGGAAGAATAATTCTCCTTTTTCGATACAAATTAAGAAAAACCGTCATTCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTg                                                                                                                               <  2:4923399/140‑1 (MQ=255)
         ccTTTTTATTCTATCCCTTTCAATAGAAGGTTTGTTACAGCCTTTTCCTTATGAATAATAATTCGCCTTTTTTTATACAAATTAAGAAAAACCGCCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTg                                                                                                                               <  1:4943795/140‑1 (MQ=255)
         ccTTTTTATTATATCACTTTCAATACAAGTTTTGTTTCAGCCTTTTCCTTTTGAATAATAATTCTCCTTTTTGGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTg                                                                                                                               <  2:2443506/140‑1 (MQ=255)
              ttcttatAGCACTTCCAATAGAAGGTTTGTTTCAGCCTTTTCCTGATGAAGAATAATTCTCCTTTTGTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGAtctc                                                                                                                          <  1:1057934/137‑1 (MQ=255)
              ttattaTCTCACTTACAATATAAGTTTTGTTTCAGCCTTTTCCTTATCAACAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCTTCAATCTTTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGAtctc                                                                                                                          <  2:1835254/140‑1 (MQ=255)
               tattatATCACTTTCAATAGAAGGTTTGTTTCAGACTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAATGTGAGAGAAACTATGCAAATCGACTCTGATCTcc                                                                                                                         >  2:1598888/1‑140 (MQ=255)
                 ttatATCACTTGCAATAGACGGTTTGTTTCAGCATTTTCATTATGAAGAATAATTACCCTTTTTTTAAACAAATTAAGAAAAACCGTAAATCTGTTTTATAATGGGTAGACAAACTATGCAAATCGACTCTGATCTCCAg                                                                                                                       <  1:2580285/140‑1 (MQ=255)
                 ttatATCAATTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAg                                                                                                                       <  2:5147635/140‑1 (MQ=255)
                   ttatCACTTTCAATAGAAGGTTTTTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGGGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:3190825/139‑1 (MQ=255)
                   atatCCCTTTCAATATATGGTTTTTTTCAGCCTTTTCCTTATTAATAATAATTCTCCTTTTTTCATAAAAATTAAGAAAAACCGTCAAACTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:3373512/140‑1 (MQ=255)
                   atatCATTTTCAATTGATTTTTTTTTTAAGCCTTTTCATTTTGATTAATAATTCTCATTTTTTTATAAAAATTAATAAAAACCTTCAATGTGTTTTATAAAGGGGATAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:755858/140‑1 (MQ=255)
                   atatCACTTTCAATATAAGTTTTGTTACAGCCTTTTCCTTATGAAGATTAATTCTACTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:5004699/140‑1 (MQ=255)
                   atatCACTTTCAATAGATGTTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGca                                                                                                                     <  1:4796083/140‑1 (MQ=255)
                   atatCACTTTCAATAGACTGTTTGTTTCAGCCTTTTCATTTTGAAGAATAATTCTCCTTTTTTGATAAAAATTAATAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGca                                                                                                                     <  1:305503/140‑1 (MQ=255)
                   atatCACTTTCAATAGACGTTTTTTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATATGTTTTATAAAGGGGAGAGACACTAGGCAACTCGACTCTGATCTCCAGca                                                                                                                     <  1:534664/140‑1 (MQ=255)
                   atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  1:449513/140‑1 (MQ=255)
                   atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  1:573319/140‑1 (MQ=255)
                   atatAACTTTCAATAGACGTTTTGTTTCAGATTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:3452480/140‑1 (MQ=255)
                   atatAACTTTAAATAGACGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTCATACAAATTAACAAAAACGGTCAATCTGTTTTATAAAGGGGAGACAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  1:718543/140‑1 (MQ=255)
                   acatCCCTATCAATAGAAGTTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                     <  2:3071430/138‑1 (MQ=255)
                    tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATCAATAATAATTCTCCTTTTTTGATACAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                                                                                                    <  1:4887573/140‑1 (MQ=255)
                    tatCACTTTAAATATAAGGTTTGTTTCAGCGTTTTCCTTATGAATAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                                                                                                    <  2:3146233/140‑1 (MQ=255)
                     atCATTTTAAATATAAGTTTTTTTTCACAATTTTCATTATTAATAATAATTCTCCTTTTTTGATAAAATTTAATAAAAACCTTCAATATGTTTTATAATGGGGTTAGAAAATATGAAAATCGACTCTGATCTCCAGcaca                                                                                                                   <  1:2784274/140‑1 (MQ=255)
                         cTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAatat                                                                                                               >  1:3621032/1‑140 (MQ=255)
                         cTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAatat                                                                                                               >  1:651421/1‑140 (MQ=255)
                              aaTAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGaaa                                                                                                          >  1:2339441/1‑140 (MQ=255)
                                   aaGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTg                                                                                                     >  2:1065287/1‑140 (MQ=255)
                                   aaGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTg                                                                                                     >  1:3809407/1‑140 (MQ=255)
                                                     ttCCTTATTAATAATAATTCTCCTTTTTTTATAAAAATTAATAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa                                                                                   <  2:1668276/140‑1 (MQ=255)
                                                     ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa                                                                                   >  1:1942186/1‑140 (MQ=255)
                                                     ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa                                                                                   >  2:179785/1‑140 (MQ=255)
                                                     ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa                                                                                   >  1:1808365/1‑140 (MQ=255)
                                                     ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAAAATATAAACATTGAAAAAAAAATTTGGGCaa                                                                                   >  2:3747113/1‑140 (MQ=255)
                                                          tATGAAGAATAATTCTCATTTTTTGATACAAATTAAGAAAAACCGTAAATATGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTACAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa                                                                              <  1:2628949/140‑1 (MQ=255)
                                                            ttattaataatTTTCCTTTTTTCATACAAATAAACAAAAACCTTAAATCTTTTTTAAAACGGGCAGACAAAGTATGAAAATCGACTCTGATGTCCAGCACAATATAGAAACATTGAAAAAAAAATTGTGGCAAAATGACg                                                                            <  2:222591/135‑1 (MQ=255)
                                                            ttaataataatTATCCTTTTTTTATAAAAATTAATAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg                                                                            <  2:4655903/135‑1 (MQ=255)
                                                            agaagaaTAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAACGGGGATAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAACTGACg                                                                            <  2:5023328/139‑1 (MQ=255)
                                                                 aataatTCTCCTTTTTTTATACAAATTAATAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                       <  2:271113/140‑1 (MQ=255)
                                                                 aataatTCTCCTTTTTTTATAAAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                       <  1:1644489/140‑1 (MQ=255)
                                                                 aataatTCTCATTTTTGGATACAAATTAATAAAAACCGTCAATCTGTTTTATAACGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACACTATAGAAACAGTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                       <  2:2927092/140‑1 (MQ=255)
                                                                 aaaaatTATCCTTTTTTGATAAAAATTAAGAAAAACCTTCACTCTGTTTTATAAAGGGGAGAGAACCTATGAAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                       <  1:2322933/137‑1 (MQ=255)
                                                                  ataatTCTCCTTTTTTGATACAATTTAACAAAAACCGTCAATCTGTTTTATAATGGGGATATAAAATATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGCGCAAAATGACGATATCa                                                                      <  2:3330831/140‑1 (MQ=255)
                                                                   taatTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCat                                                                     >  1:3918608/1‑140 (MQ=255)
                                                                       ccgcatttgttGATAAAAATTAACAAAAACCGCCATTCTGTTTTATAAAGGGGAGACAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATAttt                                                                 <  2:1747020/135‑1 (MQ=255)
                                                                           cTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGTCAAAATGACGATATCATATTTtata                                                             >  1:2844495/1‑140 (MQ=255)
                                                                           cTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTtata                                                             >  2:3974222/1‑140 (MQ=255)
                                                                           cTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCAGATTTtata                                                             >  2:5126152/1‑140 (MQ=255)
                                                                              ttttGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                         <  2:3621032/140‑1 (MQ=255)
                                                                               ttttATACAATTTATGAAAACCCGTCAATCTTTTTTATAAAGGTGAGAGACAGTTTCCAAATCGATTCTGATCTCGAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                         <  2:3650995/140‑1 (MQ=255)
                                                                               tttGATACAAATTAATAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                         <  2:229353/140‑1 (MQ=255)
                                                                                         ataaaGAAAAACCTTAAATCTGTTTTATACAGGGTATAGAAACAAATAAAATCGAAAAAGAACACCAGCACACTATATAACAATTGAAAAAAAAATTTGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGga                                               >  1:1016912/4‑140 (MQ=255)
                                                                                         aTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATGTTATACGTTTTCTTTTGga                                               >  1:3332518/1‑140 (MQ=255)
                                                                                          ttAAAAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgag                                              <  2:4512157/140‑1 (MQ=255)
                                                                                           gaacaaaaaCCTCAAATTTGTTTTATAACGGGGAGAGAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgaga                                             <  2:720620/136‑1 (MQ=255)
                                                                                            aaGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAc                                            <  2:411639/140‑1 (MQ=255)
                                                                                              taaaaaCCTTCATTCTTTTTTATAAAGGGGAGAGAAACTTTGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                          <  2:4939190/139‑1 (MQ=255)
                                                                                              caaaaaCCGTCAATCTTTTTTATAAAGGGGAGATAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGACACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                          <  2:252602/139‑1 (MQ=255)
                                                                                                   aCCGTCACTCTTTTTAATATAGTGGAGAGAAAATATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAATAAATTTGTGGCAAAATTACGATATCATATTTTATACGTTTTATTTTGGAGACAGcagac                                     <  2:1278567/140‑1 (MQ=255)
                                                                                                   aCCGTCAATCTGTTTTTTAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagac                                     <  1:1887405/140‑1 (MQ=255)
                                                                                                    ccGTCAATCTGTTTTATAAAGGGGACAGAAACTAGGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagaca                                    <  1:2376143/140‑1 (MQ=255)
                                                                                                         aaacTGTTTTATAAAGGGCAGAGAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAACTGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                               <  1:3147682/137‑1 (MQ=255)
                                                                                                         aaTCTTTTTTATAAAGGGGATAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                               <  1:3230959/140‑1 (MQ=255)
                                                                                                         aaTCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCGGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                               <  1:1718314/140‑1 (MQ=255)
                                                                                                          atattttttATAAAGGGGAGAGAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGc                              <  2:4177990/135‑1 (MQ=255)
                                                                                                            cTGTTTTATAACGGGGAGATAACCTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGt                            <  2:595078/140‑1 (MQ=255)
                                                                                                            cTGTTTTATAAAGGGTAGACAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGt                            <  2:4406502/140‑1 (MQ=255)
                                                                                                            atGTTTTATAAAGGGCAGATAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGt                            <  2:3651724/139‑1 (MQ=255)
                                                                                                              tttttATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:5083874/139‑1 (MQ=255)
                                                                                                              tttttATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATAATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:3551326/139‑1 (MQ=255)
                                                                                                              tttttATAAAGGGGAGACAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:2529837/139‑1 (MQ=255)
                                                                                                              gTTTTATACAGGGGAGAGAAACTCTGCAACTCGACTCTGATCTCCAGCACAATATAGAACCATTGAAAAAAAAATTGGGGCAACATGACGATATCATATTTTATACGATTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:991246/140‑1 (MQ=255)
                                                                                                              gTTTTATAACGGGGATAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  2:4434099/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGTAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  2:2650016/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGGAGCGAAACGAGCCAAATCGACTCTGATCTCCAGCACAAGATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  2:1991627/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:3974222/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  2:4301633/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:3388780/140‑1 (MQ=255)
                                                                                                              gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:3095821/140‑1 (MQ=255)
                                                                                                              gTTTGATAAAGGGGCCAGAAACTAGGCAAATCGACTCTGAGCTCCAGCACATTATATAAACATTGAAAAAAAAATTGGGGCAACATGACTATATCATATTTTATAATTTTTCTTTTGGAGACAGCAGACAGTGGGCGTGc                          <  2:1016912/140‑1 (MQ=255)
                                                                                                              gTTATATACAGCGGAGACAAACTATGCAAATCGACTCTGATCTCCAGCACAATATACACACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                          <  1:2814920/140‑1 (MQ=255)
                                                                                                                 ttATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtg                       <  2:4281654/140‑1 (MQ=255)
                                                                                                                 ttATAAAGGGGAGAGAACCTATGCAAATCGACTCTGATCTCCAGCACAATAGAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtg                       <  2:4566885/140‑1 (MQ=255)
                                                                                                                  tataAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgt                      <  1:2146898/140‑1 (MQ=255)
                                                                                                                         gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                       <  1:2258299/84‑1 (MQ=255)
                                                                                                                         gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat               >  1:4547327/1‑140 (MQ=255)
                                                                                                                         gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat               >  1:1342467/1‑140 (MQ=255)
                                                                                                                         gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat               >  1:1232063/1‑140 (MQ=255)
                                                                                                                         gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAAGATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGATACAGCAGACATAGGGCGTGCCTGTTGTatat               >  1:3279285/1‑140 (MQ=255)
                                                                                                                              agaAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACg          >  1:2034112/1‑140 (MQ=255)
                                                                                                                              agaAACTATGCAAATCGACTCTCATCTCCAGCACAATATCGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACg          >  1:2806610/1‑140 (MQ=255)
                                                                                                                                   cTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTg     >  1:831229/1‑140 (MQ=255)
                                                                                                                                    tATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGa    <  1:345022/140‑1 (MQ=255)
                                                                                                                                    tATGAAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGa    <  1:3692990/140‑1 (MQ=255)
                                                                                                                                     aTGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGAc   >  1:1926315/1‑140 (MQ=255)
                                                                                                                                      tGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACa  >  2:3646307/1‑140 (MQ=255)
                                                                                                                                      tGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACa  <  2:2854678/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
AAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACA  >  NZ_CP034943/3498639‑3498912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: