Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,517,425 C→A 6.5% H93N (CAT→AAT)  EO946_RS18090 → SH3 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,517,4250CA 6.5% 840.4 / 12.0 310H93N (CAT→AAT) EO946_RS18090SH3 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (148/130);  new base A (3/28);  total (151/159)
Fisher's exact test for biased strand distribution p-value = 1.88e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GATTGATTCTTATTTGGTGTCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAG  >  NZ_CP034943/3517286‑3517564
                                                                                                                                           |                                                                                                                                           
      ttCTTATTTGGTGTCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATtata                                                                                                                                       >  2:2594377/1‑140 (MQ=255)
           aTTTGGTGTCAGCATAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACCGCTTAAAAATGAATTCCATCGCAAACGCTGACTGGGCAAACGTTCATTATAAAAAc                                                                                                                                  >  1:4774694/1‑140 (MQ=255)
           aTTTGGTGTCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACGGGGCAAAGGTTCATTATAAAAAc                                                                                                                                  >  1:4056938/1‑140 (MQ=255)
                 tgtCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCaaa                                                                                                                            >  2:1776801/1‑140 (MQ=255)
                  gtCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCaaaa                                                                                                                           <  1:1081377/140‑1 (MQ=255)
                  gtCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCaaaa                                                                                                                           <  1:1262337/140‑1 (MQ=255)
                   tCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAAc                                                                                                                          <  1:105787/140‑1 (MQ=255)
                   tCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAAc                                                                                                                          >  1:3215315/1‑140 (MQ=255)
                    cagcagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACa                                                                                                                         <  1:4621305/140‑1 (MQ=255)
                     agcagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGTCAAAGGTTCATTATAAAAACGGCAAAACAg                                                                                                                        >  1:3011436/1‑140 (MQ=255)
                     agcagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAg                                                                                                                        >  1:2316412/1‑140 (MQ=255)
                       cagAGGCGTTACCTGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGc                                                                                                                      <  2:3600266/140‑1 (MQ=255)
                       cagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGc                                                                                                                      <  2:799775/140‑1 (MQ=255)
                       cagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGc                                                                                                                      <  2:4988913/140‑1 (MQ=255)
                       cagAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGc                                                                                                                      <  2:2232796/140‑1 (MQ=255)
                       cagAGGCGTTACATGTAATAACGAAGGCTTCCGCATCAAGCCAGAAAGACGATAGTCTTCACTTGGGCTAAAGCTTAAATATGATTTGCTTGGCAAACGCTGACTGGGCTAAGGTTCATTATAAAAACGGCAAAACAGGc                                                                                                                      <  2:4856175/140‑1 (MQ=255)
                         gagGCGTTACATGTCAGAAAGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCtt                                                                                                                    <  1:581915/140‑1 (MQ=255)
                         gagGCGTTACATGTCAGAAAGAAGGCGTCTGCATCAAGCAAGTAAGCTGATACGCTGCCCTTGGGCGACAGCTTAAAAGTGTTTTCCTTCGCATACGGTGATTTGGCAAATTTTCATTATTAAAACGGCATTGCAGGCtt                                                                                                                    <  2:1014741/140‑1 (MQ=255)
                          agGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCttt                                                                                                                   >  1:4278851/1‑140 (MQ=255)
                              gTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtct                                                                                                               <  2:4968339/140‑1 (MQ=255)
                              gTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtct                                                                                                               <  2:2062706/140‑1 (MQ=255)
                               ttACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtctc                                                                                                              >  1:2546711/1‑140 (MQ=255)
                               ttACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtctc                                                                                                              >  1:120219/1‑140 (MQ=255)
                               ttACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtctc                                                                                                              >  1:3591132/1‑140 (MQ=255)
                               ttACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGAAAAACAGGCTTTGtctc                                                                                                              >  2:1662606/1‑140 (MQ=255)
                                tACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTcc                                                                                                             <  1:2196115/140‑1 (MQ=255)
                                       cAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCAtt                                                                                                      >  2:1873200/1‑140 (MQ=255)
                                         gAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTAc                                                                                                    <  1:2103425/140‑1 (MQ=255)
                                          aaCGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACa                                                                                                   >  2:691108/1‑140 (MQ=255)
                                          aaCGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACa                                                                                                   >  1:927195/1‑140 (MQ=255)
                                          aaCGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACa                                                                                                   <  1:5163400/140‑1 (MQ=255)
                                          aaCGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACa                                                                                                   >  2:4928409/1‑140 (MQ=255)
                                          aaCGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTGAAACCTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACa                                                                                                   >  2:3271566/1‑140 (MQ=255)
                                             gAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTg                                                                                                >  2:2315359/1‑140 (MQ=255)
                                               aGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATaaa                                                                                                                                     >  1:3803043/1‑101 (MQ=255)
                                               aGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTa                                                                                              >  2:3398181/1‑140 (MQ=255)
                                                    tCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAagc                                                                                         <  1:2612461/140‑1 (MQ=255)
                                                    tCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACGGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATGATAAAAACGGCAAAACAGGCTTTGTCTCCCCTCATTACATTGTAAAagc                                                                                         >  2:4497511/1‑140 (MQ=255)
                                                        cATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACATCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCt                                                                                     >  2:159453/1‑140 (MQ=255)
                                                        cATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCt                                                                                     >  1:689082/1‑140 (MQ=255)
                                                         aTCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGAGTTCCCTCGCAAACGCTGACGGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTa                                                                                    <  2:2362466/140‑1 (MQ=255)
                                                            aaGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCCTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaa                                                                                 <  1:1829292/140‑1 (MQ=255)
                                                             aGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCACAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTCAAAGCAGCTacaac                                                                                >  1:4019166/1‑140 (MQ=255)
                                                             aGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaac                                                                                >  1:4376540/1‑140 (MQ=255)
                                                             aGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaac                                                                                >  1:3075711/1‑140 (MQ=255)
                                                                   aaaGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaa                                                                                 >  1:2290246/1‑133 (MQ=255)
                                                                   aaaGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaa                                                                                 <  2:3773407/133‑1 (MQ=255)
                                                                   aaaGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTacaa                                                                                 <  2:2290246/133‑1 (MQ=255)
                                                                       ccGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCaaaa                                                                                                                           >  1:4162307/1‑87 (MQ=255)
                                                                       ccGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTGCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAAcc                                                                      >  2:3056053/1‑140 (MQ=255)
                                                                          aTACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGtct                                                                                                               >  1:4993592/1‑96 (MQ=255)
                                                                                  cACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAAc                                                           >  2:1718737/1‑140 (MQ=255)
                                                                                   tattGGGCGAAAGCTAAAAACTTATTTCCTTCGCAAACGGTGACTGGGAAAATTTTCATTAAAAAAACGGAAAAACAGGGTTTGTCTCCACTCATTACTTTTTAAAAGCAGATAAAACATTAAAAACCAAAAAAAACACg                                                          <  1:174814/138‑1 (MQ=255)
                                                                                   agttGGGCTACAGCTTAAAACTTATTTCGTTCGAAAACGCTTACTGGGAAAAGGTTCATTATAAAAACGGCAAAACAGGGTTTGTCTCAAATCTTTACATTGTAAAAGCAGATAAAACATTAAAAACCAAAACAAAAACg                                                          <  1:2822809/138‑1 (MQ=255)
                                                                                    tttGGGCTACAGCTTAAAACTGATTTCCTTCGAAAACGCTTATTGGGCAAAGTTTAATTATAAAAACGGAAAAACAGGCTTTGTCTCCACTCATTCCATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAACACGa                                                         <  1:3265669/139‑1 (MQ=255)
                                                                                    tttGGGCGACAGATTAAAATTTATTTCCTTCGCAAACGCTGACTGGGAAAAGGTTCTTTATAAAAACGGCAAAACAGGTTTTGTCTCCAATCTTTACATTGTAAAAGCAGATACAACAGTAAAAACCAAAAAAAAAACGa                                                         <  1:3743663/139‑1 (MQ=255)
                                                                                    cTTGGGGGACAGCTTAAAACTGATTTCCTTCGAAAACGCTGAGTGGGCAAAGGTTCATTATAAAAACGGCAACACAGGCTTTGTCTCCACTCATTACATTTTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGa                                                         <  2:1037626/140‑1 (MQ=255)
                                                                                    cTTGGGCTACAGATTAAAACTTATTTCCTTCGAAAACGCTGACTGGGCAAAGTTTATTTATAAAAACGGCAAAACAGGCTTTGTGTCCAATCATTAATTTGTAAAAGCAGCTACAACTGTAAAAACCAAAACAAAAACGa                                                         <  1:4830395/140‑1 (MQ=255)
                                                                                    cTTGGGCTAAAGCTTAAAACTTATTTCTTTCGCAAACGCTGACTGGGAAAAGGTAAATTATAAAAACGTCAAAACAGGCTTTGTCTCCACTCATTAAATTGTAAAAGCAGCTACAACATTAAAAACCAAAACAAAAACGa                                                         <  2:2020689/140‑1 (MQ=255)
                                                                                    cTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGTTTAATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGa                                                         <  2:4016681/140‑1 (MQ=255)
                                                                                      tgtgCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGATTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:4680093/137‑1 (MQ=255)
                                                                                      tGGGCTACTGCTTAAAACTGATTTCCTTCGCAAACGCTGAATGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:3347016/140‑1 (MQ=255)
                                                                                      tGGGCTACAGGTTAAAACTTATTTCAGTCGCAAACGCTGAGTGGGAAAAGTTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAATGCAGCTAAAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:2870853/140‑1 (MQ=255)
                                                                                      tGGGCTACAGCTTAAAACTGATTTCCTTCGCAAACGCTTACTGGGCAAAGGTTCATTATAAAAAGGGCAAAACAGGCTTTTTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:562861/140‑1 (MQ=255)
                                                                                      tGGGCGACAGCTTAAATCTGATTTCCTTCGCAATCTCTGAGTGGGCTAATGTTCATTATAAAAACGGAAAACCAGGCTTTGTCTCCACTAATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:1303907/140‑1 (MQ=255)
                                                                                      tGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  2:541067/140‑1 (MQ=255)
                                                                                      tGGGCGAAAGTTTAAATCTGTTTACCTTCGAAAACGATGACTGGGAAAAGGTTCATTATAAAAACGGAAAAACTGGATTTGTCTCCACTCATTAATTTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGaaa                                                       <  1:1214354/140‑1 (MQ=255)
                                                                                       gggCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAg                                                      >  1:3499852/1‑140 (MQ=255)
                                                                                          cGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGtat                                                   >  1:3347740/1‑140 (MQ=255)
                                                                                            aCAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTcc                                                                                                             <  2:3193306/80‑1 (MQ=255)
                                                                                            aCAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGtatat                                                 >  1:2662036/1‑140 (MQ=255)
                                                                                            aCAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGtatat                                                 >  2:3119331/1‑140 (MQ=255)
                                                                                            aCAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGtatat                                                 >  2:2134989/1‑140 (MQ=255)
                                                                                            aCAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGtatat                                                 >  1:4552338/1‑140 (MQ=255)
                                                                                               gCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACa                                              >  2:3849706/1‑140 (MQ=255)
                                                                                                   aaaaCTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCg                                          <  2:4547649/140‑1 (MQ=255)
                                                                                                   aaaaCTGATTTCCTTCCCAAACGATGACTGGGAAACTTTTAATAAAAAAAACGGAAAAACAGGCTTTGTCTCCACTCATAACATTTTAAAAGCAGCTAAAACAGAAAAAACCAAAACAAAAACGAAAGTATATACATCCg                                          <  1:1873200/140‑1 (MQ=255)
                                                                                                          aTTTCCTTCTCAAATGCTGACTGGGCAAAGGTTAAGTATAAAAATGGAAAAACAGGATTTGTATCCACTCATTACATTGTTAAAGCAGCGACAACGGTAAAAACCAAAACAAAAACAAAAGTGTATACATCCGCTGACgg                                   >  1:141697/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAACACGAAAGTATATACATCCGCTGAAgt                                   >  2:2655776/1‑139 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAgg                                   >  1:3523514/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAgg                                   >  2:4788116/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAgg                                   >  2:2808875/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAgg                                   >  1:959160/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAgg                                   >  1:161946/1‑140 (MQ=255)
                                                                                                          aTTTCCTTCGCAAACGCTGACGGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCAGCTGAAgg                                   >  1:5087749/1‑140 (MQ=255)
                                                                                                              ccTTCGCAAACGCTGAGTGGGCAACTGTTCATTATAAAAACGGCAACACAGGCTTTGTCTCCACTCATTACATTTTAAAAGCAGCTACAACAGTAAAAACAAAACCAAAAACGAAAGTATATACATCCGCTGAAGGaaaa                               <  2:4145342/140‑1 (MQ=255)
                                                                                                              ccTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGaaaa                               <  2:1120423/140‑1 (MQ=255)
                                                                                                                   gCAAACGCTTAGTGGGCAAAGTTACATTATAAAAACGGCAAACCAGGATTTGTCTCCACTCATTAAATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCAt                          <  2:2546711/140‑1 (MQ=255)
                                                                                                                   gCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTAAAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCAt                          <  2:3499725/140‑1 (MQ=255)
                                                                                                                         gatGACTGGGCAAAGGTAAATTATAAAAACGGCAAAAAAGGCTTTGTCTCCACTCATTACATTTTAAAAGAAGCTAAAAAAGTAAAAACAAAAACAAAACCGAAAGTATATACATCCGCTGAAGGAAAAATCATCAacac                    <  2:138740/138‑1 (MQ=255)
                                                                                                                         gCTGACTGGGCAAAGGTTAATTATAAAAACGGAAAAACAGGCTTTGTCTCCACTCTTTACATTGTAAAAGCAGATACAACCGTAAAAACCAAAACAAAACCGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGAc                    <  2:648903/140‑1 (MQ=255)
                                                                                                                              cTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTc               <  2:5078963/140‑1 (MQ=255)
                                                                                                                                 ggCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCcag            >  2:1520842/1‑140 (MQ=255)
                                                                                                                                   cAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAACCGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCcagca          <  2:3552212/140‑1 (MQ=255)
                                                                                                                                   cAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCcagca          >  2:4614263/1‑140 (MQ=255)
                                                                                                                                   cAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCcagca          <  2:120219/140‑1 (MQ=255)
                                                                                                                                           cATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAg  >  1:2425365/1‑140 (MQ=255)
                                                                                                                                           cATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAg  >  1:4596843/1‑140 (MQ=255)
                                                                                                                                           cATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAg  >  2:2434713/1‑140 (MQ=255)
                                                                                                                                           cATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAg  >  1:2469708/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GATTGATTCTTATTTGGTGTCAGCAGAGGCGTTACATGTCAGAACGAAGGCTTCCGCATCAAGCCAGAAAGCCGATACGCTGCACTTGGGCGACAGCTTAAAACTGATTTCCTTCGCAAACGCTGACTGGGCAAAGGTTCATTATAAAAACGGCAAAACAGGCTTTGTCTCCACTCATTACATTGTAAAAGCAGCTACAACAGTAAAAACCAAAACAAAAACGAAAGTATATACATCCGCTGAAGGAAAAAGCATCAAGACTCTTCCAGCAGAGACAAG  >  NZ_CP034943/3517286‑3517564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: