Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,539,638 G→T 8.3% P105P (CCC→CCA EO946_RS18215 ← T7SS effector LXG polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,539,6380GT 8.3% 584.2 / 7.2 238P105P (CCC→CCAEO946_RS18215T7SS effector LXG polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base G (88/119);  new base T (26/3);  total (115/123)
Fisher's exact test for biased strand distribution p-value = 1.38e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

TGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGT  >  NZ_CP034943/3539501‑3539776
                                                                                                                                         |                                                                                                                                          
tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                                                                          <  1:45940/140‑1 (MQ=255)
tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                                                                          <  2:1412091/140‑1 (MQ=255)
tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                                                                          <  2:1108647/140‑1 (MQ=255)
tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                                                                          <  1:3846531/140‑1 (MQ=255)
tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                                                                          <  1:1882298/140‑1 (MQ=255)
ggggCATCGCCCATCGTCTCGGCCTAGTCGTCACGCGTGTACATGTCGAGATCAATACTATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCCTTggg                                                                                                                                          <  1:2673499/139‑1 (MQ=255)
 gggCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGc                                                                                                                                         <  2:1289011/140‑1 (MQ=255)
              cTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTTAGCGGAGCAAAATTCTGAGGGTTTGAAGTCTCCCTTGGGGCGCTGATGATCCa                                                                                                                            >  1:4183745/1‑140 (MQ=255)
              cTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCa                                                                                                                            <  2:3706255/140‑1 (MQ=255)
              cTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCa                                                                                                                            <  2:4328653/140‑1 (MQ=255)
                 aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATTAGCGGAGACAAATTCTGAGGCTTTCAAGTCGCCATTGGGCAGCTCATGATACaaaa                                                                                                                         >  1:5030395/1‑140 (MQ=255)
                 aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTATTTATGAGGGGAGTCAATTTCTGAGGGTTTCAATTATGCATTGGGCAGCTCATGATACaaaa                                                                                                                         >  1:4959783/1‑140 (MQ=255)
                 aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATGTATTGTTTAAGATCGGACACAAATTATGAGGCTTTTAAATATTCAATTGGCCTCTCATGATACaaaa                                                                                                                         >  1:4740440/1‑140 (MQ=255)
                  tCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGGTGATGCCTGACAAAATGGAGTCGATTGGTTGTTTATGGGCGGCGACAATTTCTGAGGCGTTGACGGCTGCATTGGGGCGCTCATGATCCcaaaa                                                                                                                        >  2:4038188/1‑140 (MQ=255)
                  tCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGGTGTTTAATTGCGGGTACCATTTGTTAGGCGTTCAAATGTCCAATGGGCAGCTCATGATACaaaaa                                                                                                                        >  1:62761/1‑140 (MQ=255)
                  tCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCaaaaa                                                                                                                        <  2:3061577/140‑1 (MQ=255)
                   cGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGATCGGAGACAATTTCTGAGGCGTTCAAGTCTGCATTGGGCCGCGAATGATCCaaaaaa                                                                                                                       >  2:3985666/1‑140 (MQ=255)
                   cGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTATTTATGAGCGGAGACAATTTCTGAGGCTTTGAAGTCTCCCTTTGGCAGCTCATGATCCaaaaaa                                                                                                                       >  2:120955/1‑140 (MQ=255)
                    ggCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaag                                                                                                                      <  1:2198642/140‑1 (MQ=255)
                      cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCGTTATGATCGGAGACAAATTCTTAGGCTTTAAACTCAACAATGTGCAGCACATGATACAaaaaaaaa                                                                                                                    >  1:245295/1‑140 (MQ=255)
                      cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGGTGTTTATGAGCGGGGACAATTTGTGAGGCGTTGAAGTCGCCATTTGGCCGCGCCTGACCCAAAAaagaa                                                                                                                    >  1:744021/1‑140 (MQ=255)
                      cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGAGCGGAGACAAATTATGGTGCTTTGAACTATGCATTTGGCACCTCAAGATACAAAAaagaa                                                                                                                    >  1:1562563/1‑140 (MQ=255)
                        tAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGt                                                                                                                  <  2:260356/140‑1 (MQ=255)
                        tAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGt                                                                                                                  <  2:382288/140‑1 (MQ=255)
                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                               <  2:522085/140‑1 (MQ=255)
                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                               <  1:3294662/140‑1 (MQ=255)
                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTAAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                               >  2:4959224/1‑140 (MQ=255)
                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTATGAGCGGCGACAATTTCTGAGGCTTTCAAGTCTCCAATTGGCCGCTCATGATCCAAAAAAGAAGttta                                                                                                               >  2:2209513/1‑139 (MQ=255)
                           tcggcACGCGTGGACCTCTCGAGCTCCATACGACCGCGGATGCCTCACAAACTGGAGTCGATGTCGACGTTAGGCGCGGAGACAAGTTCTGCGGCTTTCAAGTCGCCATTGGGCAGCTCATGATCCAAAACAGAAGtttg                                                                                                               <  2:1388234/140‑2 (MQ=255)
                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTTTTATCCGAGACAAATTGTTTGGCGTTGTAGTGTCCATTGGGCCGCGCGTGGTCCAAAAAAGAAGtttgccccg                                                                                                          >  1:355335/1‑134 (MQ=255)
                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                                                                          <  2:2680697/140‑1 (MQ=255)
                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                                                                          <  1:4349949/140‑1 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTGAGGGGAGAACAGTTATGATGCTTTCGAGGCGGCATTGGGCATCTGATGATACAAAAAAGAAATTTCGACAAAAGaa                                                                                                     >  1:4825907/1‑140 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTGTATTATCGGAGACAATGTCTGTGGGTTTGAAGTCTCCATTGGGCAGCTGCTGATGCAAAAAAGTAGTTTCCCCATATGaa                                                                                                     >  1:2276946/1‑140 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTTTGAGGGGAGACAATTTTTTAGGGTTTGAAGTCTCCATTGGGGAGCTAATGATCCAAAAAAGAAATTTACACATATGaa                                                                                                     >  1:3868249/1‑140 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTATTTATGATCGGAGAAAATTTCTGAGGCTTTCAACTCTGCATTTTGCAGCTCATTATCCAAAAAAGAAATTTACACAAATTaa                                                                                                     >  2:591397/1‑140 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGGTGTTTATGAGCGGTGACCAGTTCTGAGGCTTTCAACTGGGCACTGGGCACCTCATTGTGCCAAATATAAGGTTCCACCTATGaa                                                                                                     >  1:2669547/1‑140 (MQ=255)
                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                                                                     <  1:1351313/140‑1 (MQ=255)
                                       tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATTTGCGGAGACAAATTCTTAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATACAAAAAAGAAGTTTCCACATAAGAATc                                                                                                   >  1:3884678/1‑140 (MQ=255)
                                          atctCGACATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCATTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAACAAGAAGTTTCCACATATGAATCAtt                                                                                                <  1:1006813/137‑1 (MQ=255)
                                          aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                                                                                <  1:4700022/140‑1 (MQ=255)
                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTTTGCGGCGACAAATTGTGATGCTTTAAAGTCTCCAATTGGCAGCTCATGTTACAAAAAATAAGTTTGGACATAAGAATAATTCa                                                                                              >  1:3470100/1‑140 (MQ=255)
                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATGATCGGAGAAAAATTATGATGCTTTCAAGTCTCCATTGGGCAGCTCATTATACAAAAAATAACTTTCCAAAAAAGAATAATTCa                                                                                              >  2:2552898/1‑140 (MQ=255)
                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCGTGGTTAGGAGCGGAGACAAATTCTGAGGCTTTCAAGGATCCCTTGGGCCGCTCATGATCCAAAAAAGAAATTTACACAGATGAATCATTcc                                                                                              >  2:1986058/1‑139 (MQ=255)
                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCGTCTTTATGAGCGGAGACAAATTATGAGGCTTTCAAGGCTCCATTGGGGCCCGCATGATCCAAAAAAGAAGTTTCCAAAAATTAATAAATCa                                                                                              >  2:101198/1‑140 (MQ=255)
                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGGTGTTTTTTAGCGGAGTCAATTTCTGAGGCTTTGAATTCTCCATTTGGCAGCTCATTATCCAAAAAAGAAGTTTCCACATAATAATCAttaa                                                                                              >  1:4518821/1‑138 (MQ=255)
                                               gagaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGtt                                                                                           <  1:1252995/140‑1 (MQ=255)
                                                  aacAATAATATCGCTGATGCCTGACAAAATGGAGTCTATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                        <  2:3444886/138‑1 (MQ=255)
                                                  aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                        <  1:2383875/140‑1 (MQ=255)
                                                  aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTAATTATCCAAAAAAGAAGTTTCCACATATGAATAAATAAGTTAtt                                                                                        >  2:2393393/1‑140 (MQ=255)
                                                  aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATTTGCGGAGACAATTTGTGAGGCTTTGAAGTGTACATTTGGCAGCTCATGATCCCAAAAAGAAGTTTACACAAAATAAACATTCAGTTAtt                                                                                        >  1:1306647/1‑140 (MQ=255)
                                                   tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                                                                       <  1:1431373/140‑1 (MQ=255)
                                                   tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                                                                       <  2:4814586/140‑1 (MQ=255)
                                                     aataatATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGAt                                                                                     <  1:2861442/140‑1 (MQ=255)
                                                     aataatATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGAt                                                                                     <  1:4009369/140‑1 (MQ=255)
                                                      ataataTCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGGTTATGAGCGGCGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCAAGATACAAAAAAGAAGTTTACACAAATGAAACATTGAGGTGTTGATc                                                                                    >  1:3631557/1‑140 (MQ=255)
                                                            tCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTGAGCGGGGACCATTTGTGAGGCTTTGAAGTCTCCCATGGGCAGCTCATGATCCAAAAAAAAACTTTGCACAAATTAATCATTGAGTTCGTGTTCTTTAAt                                                                              >  1:5076539/1‑140 (MQ=255)
                                                            tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                              <  1:3675948/140‑1 (MQ=255)
                                                              gCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGCCTTTCAAGTCTCCATTCGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGt                                                                            <  2:278036/140‑1 (MQ=255)
                                                              gCTGATGACTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGt                                                                            <  2:2350003/140‑1 (MQ=255)
                                                              gCTGAGGCCTGACAAAATGGAGTCGAGTTCTTCGTTATGAGCGGCGACAAGTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCGTGATCTGTAATGt                                                                            <  2:356263/140‑1 (MQ=255)
                                                               cTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGtc                                                                           <  2:4477833/140‑1 (MQ=255)
                                                                  aTGCCTGACAAAATGGAGTCGATTTGGTGTTTATGAGCGGTGACAATTTCTGAGGCTTTGAAGTGTCCCTTGGGCAGCTCATGATACAAAAAAGAAGTTTCCACATATGAATGATTGAGTTGTTGATCTTTAATGtgtgc                                                                        >  2:5050344/1‑136 (MQ=255)
                                                                    gCCTGACAAAATGGAGTCGATTTCTTCTTTTTGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCCGCTCATTATCCAGAAAAGAAGTTTACACATATTAATCATTCAGTTGTTGTTCTTTAATGTCTCCTg                                                                      >  2:4783592/1‑140 (MQ=255)
                                                                    gCCTGACAAAATGGAGTCGATTTCTTCGTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTg                                                                      >  2:4099286/1‑140 (MQ=255)
                                                                     ccTGACAAAATGGAGTCGATTTGGTGTTTATGAGCGGAGACAATTTCTGAGGGTTTCAAGTCTCCAATTGGGAGCGCAAGATCCAAAAAAGAAGTTTCCCCCTATGAATCATTGAGTTCTTGGTCTTTAATGTCTCCGGa                                                                     >  2:3125656/1‑140 (MQ=255)
                                                                         aCAAAATAGAATCGATTTCTTCTTTATGAGCGGAGACAATCTCTGAGGCTTTCAGGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATATCTCCTGAAATg                                                                 <  2:4580626/140‑1 (MQ=255)
                                                                           aaaaTGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGcc                                                               <  2:2132211/140‑1 (MQ=255)
                                                                              aTGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCAtt                                                            <  2:1642334/140‑1 (MQ=255)
                                                                                          tcttctTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAt                                                <  2:1855268/140‑1 (MQ=255)
                                                                                            ttcttTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAttt                                              <  2:3213409/140‑1 (MQ=255)
                                                                                                ttATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCATCTCATGATCCCAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTTATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                          <  2:609663/140‑1 (MQ=255)
                                                                                                ttATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                          <  1:1527047/140‑1 (MQ=255)
                                                                                                ttAGGAGCGGACACAATGTCCGAGGCTTACAAGTCTCCATTTGGCAGCTCATGAACCAAAACAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAATACGCAATTTGAGc                                          <  2:5164053/140‑1 (MQ=255)
                                                                                                 tATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCa                                         <  1:2036409/140‑1 (MQ=255)
                                                                                                 tATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCa                                         <  1:423447/140‑1 (MQ=255)
                                                                                                    gAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCagag                                      >  1:2354769/1‑140 (MQ=255)
                                                                                                      gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                    <  1:1904287/140‑1 (MQ=255)
                                                                                                      gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                    <  1:3401436/140‑1 (MQ=255)
                                                                                                      gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                    <  2:4694721/140‑1 (MQ=255)
                                                                                                      gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                    <  1:4906959/140‑1 (MQ=255)
                                                                                                      gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                    <  1:1191116/140‑1 (MQ=255)
                                                                                                        ggAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACaa                                  <  2:644811/140‑1 (MQ=255)
                                                                                                        ggAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACaa                                  <  2:3272832/140‑1 (MQ=255)
                                                                                                        ggAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGAACTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACaa                                  <  2:1184721/140‑1 (MQ=255)
                                                                                                        ggAGACAAATTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACaa                                  <  2:4239195/140‑1 (MQ=255)
                                                                                                                tttCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTATCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTcagc                          <  1:4640278/140‑1 (MQ=255)
                                                                                                                 ttCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTGCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTcagcc                         >  2:3870681/1‑140 (MQ=255)
                                                                                                                  tCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTcagcca                        >  1:4104334/1‑140 (MQ=255)
                                                                                                                      aGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCtt                    >  1:2776266/1‑140 (MQ=255)
                                                                                                                        gaatacAAGTCTCCATTGTGAATATAATGATAAAAAAAATAAGTTTCCACATATGAATCATTCAGTTCTTGATATTTAATGTCTCCTGAAATGACATTTAAAAACTCAATTTGAGCATAGACAAGTGTCAGCCAGAttta                  >  2:4667159/6‑139 (MQ=255)
                                                                                                                          tttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                <  1:244027/140‑1 (MQ=255)
                                                                                                                          tttCAAGTCTCCAGTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                <  1:909040/140‑1 (MQ=255)
                                                                                                                           ttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcgac               >  1:586483/1‑140 (MQ=255)
                                                                                                                           ttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcgac               >  1:4344453/1‑140 (MQ=255)
                                                                                                                           ttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcgac               >  1:3166008/1‑140 (MQ=255)
                                                                                                                               aGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGAtt           <  1:890285/140‑1 (MQ=255)
                                                                                                                                   tcCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAATTGTAAGCCAGCTTTCGACGATTTCCg       >  2:1048028/1‑140 (MQ=255)
                                                                                                                                   tcCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACCCAATTTGAGCACAGACAACTGTCAGCCACCTTTCGACGAATTCCg       >  2:1564636/1‑140 (MQ=255)
                                                                                                                                        tGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGt  >  1:1076470/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGT  >  NZ_CP034943/3539501‑3539776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: