Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,291 G→T 5.3% S324S (TCG→TCT EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,2910GT 5.3% 1188.7 / 6.4 445S324S (TCG→TCTEO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (198/201);  new base T (2/43);  total (201/244)
Fisher's exact test for biased strand distribution p-value = 4.08e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTA  >  NZ_CP034943/3584152‑3584429
                                                                                                                                           |                                                                                                                                          
tgACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCg                                                                                                                                            <  2:77430/140‑1 (MQ=255)
    aGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCAGTAATGCCTTGACAAGCCGAGGACGCATGTCCTCAAGTAGCAACTGCTGATTTATCGTTaa                                                                                                                                        >  1:3160088/1‑140 (MQ=255)
      ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAc                                                                                                                                      >  2:2730879/1‑140 (MQ=255)
        aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACACGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    <  1:1008098/140‑1 (MQ=255)
        aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAACCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    <  2:3095809/140‑1 (MQ=255)
          ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                                  <  1:4480472/140‑1 (MQ=255)
          ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                                  <  2:1452601/140‑1 (MQ=255)
             gAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCa                                                                                                                               <  1:1574603/140‑1 (MQ=255)
              aaTATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAt                                                                                                                              >  2:124741/1‑140 (MQ=255)
                tataAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTa                                                                                                                            >  2:3327841/1‑140 (MQ=255)
                  taAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTaca                                                                                                                          <  1:4275659/140‑1 (MQ=255)
                  taAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTaca                                                                                                                          <  1:2697773/140‑1 (MQ=255)
                     aaaaaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACAccc                                                                                                                       >  1:4074230/1‑140 (MQ=255)
                     aaaaaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACAccc                                                                                                                       >  1:2150901/1‑140 (MQ=255)
                                aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                            <  1:819199/140‑1 (MQ=255)
                                     tACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTc                                                                                                       >  1:4626937/1‑140 (MQ=255)
                                          tGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGa                                                                                                  >  2:2131661/1‑140 (MQ=255)
                                          tGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGa                                                                                                  >  2:4246079/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:4413815/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                           <  2:3717542/97‑1 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                            <  2:2678763/128‑1 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  2:2825745/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                <  2:3273296/140‑1 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:4548128/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  2:4089823/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:117088/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:225920/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:2850729/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:2940691/1‑140 (MQ=255)
                                                  aCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCCAGTAGCAACTGCTGATTTATCGTTACACCAGCCCATTACACCCAACGAAATAAAAAGTCTCAGACTTGAGAt                                                                                          >  1:3281234/1‑140 (MQ=255)
                                                    aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                        <  2:4841895/140‑1 (MQ=255)
                                                      tcTCCAAAAGATGCGGAAATGAGAATGCATGAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAACTAAAAAGTCTAAGAATTGCGATTGCa                                                                                      <  1:4032855/139‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGACTGCATTAATGCCTTTACAAGCCGACGACGCATTTCCTCAAGTATCAACTGCTGATTTATCGTTAAACCAGCCCATTAAACCAAACGAAAAAAAAAGTCTAATAATTGAGATTgaa                                                                                      >  2:5015956/1‑138 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGGCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGGTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCAAAGAATTGCGATTGCa                                                                                      <  1:3881647/140‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAATGTCTAAGAATTGCGATTGCa                                                                                      >  1:2375279/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                        <  2:1079156/138‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:1548382/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  2:2508140/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:3194391/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:4189380/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAGTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:3438623/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATCAGAATGCATTAATGCCTTTACAATCCGAGGAATCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAATTATAAGAATTGAGATTTCa                                                                                      >  2:1172149/1‑140 (MQ=255)
                                                         ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                   <  2:1792756/140‑1 (MQ=255)
                                                         ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                   <  1:3640581/140‑1 (MQ=255)
                                                           aaaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTAGCGTTAAACCAGCCCATTACACCCAACGTAATAAAAAGTCTATGAATTGCGATTGCAGTGat                                                                                 >  1:887583/1‑140 (MQ=255)
                                                            aaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGata                                                                                >  1:1875863/1‑140 (MQ=255)
                                                              aGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                              <  1:3803421/140‑1 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                          >  1:3543755/1‑140 (MQ=255)
                                                                           agaATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCg                                                                 >  1:4093563/1‑140 (MQ=255)
                                                                               tGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    >  1:4948367/1‑69 (MQ=255)
                                                                               tGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    <  2:452137/69‑1 (MQ=255)
                                                                                 cATTAATTCCTCTACAAGCCGAGGACGCATTTCCTAAAGTAGCAAGTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAATTATAACAATTGCGTTTGCATTAATATGTATGCTAAACTCGACTCGt                                                           <  2:73697/140‑1 (MQ=255)
                                                                                       tGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATAGACTCGTCTGcag                                                     >  2:5064338/1‑138 (MQ=255)
                                                                                           tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaa                                                 >  2:608993/1‑140 (MQ=255)
                                                                                            ttACAATCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTATGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                >  1:2093134/1‑140 (MQ=255)
                                                                                            ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                >  2:1496111/1‑140 (MQ=255)
                                                                                                caGCCGCGGACGCATTTCCTCAAGTAGCAACTGCTGTTTTATCGTTAAACCCGCCCATGCCACCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAgcg                                            <  1:4675674/139‑1 (MQ=255)
                                                                                                aaGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAcaa                                                                                                                                    >  2:3938877/1‑50 (MQ=255)
                                                                                                  gCCGAGTACGCATTTCCAAAAGTAGAAATTTCTGATTTATCTTAAAACCAGCCCTTTACACCCAACTAAATAAAAAGTCTAATATTTGCTATTGAAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCgct                                          <  2:4189380/140‑1 (MQ=255)
                                                                                                  gCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgct                                          >  2:1609920/1‑140 (MQ=255)
                                                                                                    cGAGGACGCATTTCCTCAAGTAGCACCTGATGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGATTTGCTTTTGAAGTGATAGGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                        <  2:376983/140‑1 (MQ=255)
                                                                                                    cGAGCACGCATTGCCTCAAGTAGCAACGGCTGATTTATCGTTACACCCGCCCATTACACCCCACGAAATAACAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                        <  2:3070657/140‑1 (MQ=255)
                                                                                                      cggccGCTTTTCCTCATTTAGAAACTGCTTTTTTATTTTTAAACACGCCCTTTACACCAAACTAAATAAAAATTATAATATTTGATTTTGCTTTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  1:2233219/136‑1 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  2:4074230/140‑1 (MQ=255)
                                                                                                      aGGACGAATTTCCTCAATTAGAAATTGCTTATTTATCGTTAAACCAGCCAATTACACCAAACGAAATAAAAAGTCTAAGATTTGCTTTTGCTGTGATTTGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  1:2325693/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAATTACCAACTGCTTATTTATCTTTAAACCACCCCATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGTTTGCAGTTATATTTAGGATAAAATCGTCTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:5142757/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  2:2251291/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAAGTAGAAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:3704958/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTAATTTAGCAAGTGCTTATTTATGTTTAAACCAGCCCATAACACCCAACTAAATAAAAAGTCTAAGAATGGCGATTGAAGTGATTTGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:124741/140‑1 (MQ=255)
                                                                                                       gcaCGCATTCCCTCATGTAGCAAGTGTTTATTTATCGTTAAACCCCCCCATAACACCCAACCAAATAAACACTCTAAGAATTCCTATTGCAGTTTATTGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:2571764/138‑1 (MQ=255)
                                                                                                       cgACGCATTTCCTCAAGTAGCAACGGCTGATTAATCGTTAAACCCGCCCATTACACCCACCGAAATAAACCGTCAAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:3942905/139‑1 (MQ=255)
                                                                                                        gACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTccc                                    >  1:4588647/1‑140 (MQ=255)
                                                                                                           gCATTTCCTCATGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAACCTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:4757247/140‑1 (MQ=255)
                                                                                                           gCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:4976052/140‑1 (MQ=255)
                                                                                                           gCATTTCCACAAGTAGAAACTGCTGATTTATCGTTAAACCAGCCCATTACACCAAACGAAATAAAAAGTATAAGAATTGCGATTGCAGTGAAATTTTGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:186286/140‑1 (MQ=255)
                                                                                                           gCATTTCATCAAGTAGCACCTGCTTATTTATCTATAACCCAGCCCATTACACCCAACCAAACAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCGGCTCCCTAt                                 <  1:4205805/140‑1 (MQ=255)
                                                                                                            cATTTCCTCAATTAGAAACTGCTTTTTTATCTTTAAACCAGCCCATTACACCCACCGAAATAAAACGTCTAATAATTGCGATTGCTGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                <  2:891641/140‑1 (MQ=255)
                                                                                                             aTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                              >  2:3187334/1‑93 (MQ=255)
                                                                                                              tttCCTGAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCTTTACACCCACCGAAATAAAATGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  2:3645888/140‑1 (MQ=255)
                                                                                                              tttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  2:4336009/140‑1 (MQ=255)
                                                                                                              tttCCACAAGTAGAAACTGCTTATTTATCGTTCAACCAGCCCATTACACCCAACGAAATAAAAAGTCTACGAATTGCGATGGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  2:5177692/140‑1 (MQ=255)
                                                                                                              tttACTAAAGTATAAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGTTTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  2:4573079/140‑1 (MQ=255)
                                                                                                               ttCCTCATGTATCATCTGCTGATTTATCGTTAATCCAGCACATTACACCCACGTTAATAATAAGTATACTAATTGCGTTTGTTGTTATTTGTATGGTAAATTCGAGTCGTCTGCAGAAAAAAGCGCTGCTCCCTATTTaa                             <  2:294480/140‑2 (MQ=255)
                                                                                                               ttCCTCAAGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGTTTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  2:4624306/140‑1 (MQ=255)
                                                                                                               gacatCAATTAGCAAAGGCTGTTTTATCGTTAAACCAGCCCATTCCACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGACGTGATTTGTAGGAGAACCTCGACTCGTATGCCGAAAAAAGCGCTGCTAAATATTTAc                             <  2:2281638/136‑1 (MQ=255)
                                                                                                                tCCTCATGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTCTAATAATTGCTTTTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACg                            <  1:1496111/140‑1 (MQ=255)
                                                                                                                tCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACg                            >  1:1759999/1‑140 (MQ=255)
                                                                                                                 ccTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACgg                           >  1:1681978/1‑140 (MQ=255)
                                                                                                                 ccTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATGGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACgg                           <  1:1436795/140‑1 (MQ=255)
                                                                                                                 ccTAAAGTACCAACTGATGATTTATCGTTAAACCCCCCCATAACCCCCACCAAAATAAAAAGTCTACCAATTGCGATTCCATTGATATGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACgg                           <  1:2007749/140‑1 (MQ=255)
                                                                                                                  ctcttgTAGCACCGTCTGTTTTTTCGTTAAACCAGCCCTTTACCCCCACCGACATATAATGTCTAAGATTTGCGTTTGCTGTGATTTTTTGGCTAAAATCGAATCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  2:1970482/135‑1 (MQ=255)
                                                                                                                    cAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                        <  2:2155872/140‑1 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                     <  2:3526523/140‑1 (MQ=255)
                                                                                                                            aaCTGCTTATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATGGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTg                <  1:705385/140‑1 (MQ=255)
                                                                                                                            aaCTGCTGATTTATCTTTAAACAAGCCAATTACACCCAACGAAATAAAAAGTATAAGAATTGCGATTGCAGTGATATTTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTg                <  1:4828960/140‑1 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAACCCAGCCCATTACACCCACCGAAATAAAAAGACTACGACTTGCGATGGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa           <  1:3090908/140‑1 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa           >  1:1210683/1‑140 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCGGTTGaa           >  1:3060586/1‑140 (MQ=255)
                                                                                                                                    aTTTATCGTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTCTAATAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGc        <  2:296533/140‑1 (MQ=255)
                                                                                                                                       tATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGt     >  2:1391728/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTA  >  NZ_CP034943/3584152‑3584429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: