Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,719,864 T→G 7.9% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,719,8640TG 7.9% 984.5 / 16.3 454S79R (AGC→CGC) EO946_RS19145YwdI family protein
Reads supporting (aligned to +/- strand):  ref base T (163/234);  new base G (2/54);  total (165/289)
Fisher's exact test for biased strand distribution p-value = 1.25e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 456 total aligned reads displayed.

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001
                                                                                                                                           |                                                                                                                                         
    cTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTccc                                                                                                                                       <  1:3759649/140‑1 (MQ=255)
      gTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcat                                                                                                                                     >  2:5081714/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTcgaatg                                                                                                                                    >  1:2099593/1‑135 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:3480834/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:964226/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  1:1056357/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  1:3406274/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  1:4942608/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTTCCCATCAtttt                                                                                                                               >  1:1664658/1‑140 (MQ=255)
              cAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttttt                                                                                                                             <  2:158317/140‑1 (MQ=255)
              cAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttttt                                                                                                                             <  1:4437880/140‑1 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:2040331/1‑140 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTGTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  1:3306374/1‑140 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        <  2:1243562/140‑1 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        <  2:4073878/140‑1 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  2:3564755/1‑140 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTACGTCCTCTTCCTGCTGCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  1:3659205/1‑140 (MQ=255)
                     ccAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTGTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatcat                                                                                                                      <  2:1046671/140‑1 (MQ=255)
                      cAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCatcatc                                                                                                                     >  2:822093/1‑140 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:518552/140‑1 (MQ=255)
                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  2:2783858/1‑140 (MQ=255)
                                    acaTAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGa                                                                                                       >  2:2168306/1‑140 (MQ=255)
                                     caTAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGAt                                                                                                      <  2:4266082/140‑1 (MQ=255)
                                          cTCAGCCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  2:3270730/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATACCGCCTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGAGGGCgg                                                                                                 <  2:3397458/140‑1 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  2:3652597/140‑1 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  2:3969986/140‑1 (MQ=255)
                                                cTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGc                                                                                           <  1:4247822/140‑1 (MQ=255)
                                                cTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGc                                                                                           <  1:4827450/140‑1 (MQ=255)
                                                  ttCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGGGGTGCGGccg                                                                                         >  2:1476300/1‑138 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  2:724164/1‑140 (MQ=255)
                                                       aTTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTc                                                                                    >  2:750058/1‑140 (MQ=255)
                                                        ttAAAAATCAAAGAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGGCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCg                                                                                   <  2:456984/140‑1 (MQ=255)
                                                           aaaaTCAACTAAAGACTCTCCGTTTCCGTCCGCGTCCTGCTTCTCTTTCGCCTGCTTTCGATATGTGTTTAATCCCGCGCTTCCCATCATTTTTTCCAGCATCCGCTGGTCTGTCGAGGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:3535749/140‑1 (MQ=255)
                                                           aaaaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:4902634/140‑1 (MQ=255)
                                                             aaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  2:2453484/140‑1 (MQ=255)
                                                             aaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  1:3043262/140‑1 (MQ=255)
                                                             aaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  1:2185253/140‑1 (MQ=255)
                                                                cAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtgctg                                                                           <  1:678260/140‑1 (MQ=255)
                                                                  aatcacgACTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:3128188/134‑1 (MQ=255)
                                                                  aataatGACTCTCAGTTTCCGTCCTCTTTCTGGTTCTCTTTATCGTTATTTCGATATTTGTTTAATCGAGCGGGTCCGTTCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCTGCAGGACTCGGAGTggtgct                                                                         <  2:3816396/140‑1 (MQ=255)
                                                                  aagaaaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2708201/137‑1 (MQ=255)
                                                                  aaTAACGACTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:2373350/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:1839030/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:1464068/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:1825886/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:750058/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:668171/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:601444/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:3830860/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:5020168/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:3959494/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:3224971/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:4091558/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATGTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4043448/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCCGCTTCTCTTTCTCCTGCTTTCGAGATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2884437/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGGGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:2321499/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGATGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2040331/140‑1 (MQ=255)
                                                                      acgtctctCCGTTTCCGGCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGTGGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  2:2545399/136‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  1:1022451/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGATTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  2:3908861/140‑1 (MQ=255)
                                                                            ctcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAg                                                                                                                   >  1:2447633/1‑88 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4647551/129‑1 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         >  1:5153922/1‑129 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:2947876/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:1134549/1‑140 (MQ=255)
                                                                                 tttCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAGTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgggg                                                          <  1:857197/140‑1 (MQ=255)
                                                                                 gttCCGTCCTCGTCCTGCTTCTCTGTCTCCTGCTGTCGATATTTGTTTAATCCCGCGCTTCCCATCAGTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgggg                                                          <  2:3031301/139‑1 (MQ=255)
                                                                                   tCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGgcg                                                        >  2:2917375/1‑140 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  2:1362288/1‑140 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:31020/140‑1 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTCCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:4986713/140‑1 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:4900256/140‑1 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:4586806/140‑1 (MQ=255)
                                                                                         ctctTCCTGCTTCTCGTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCGGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:3874809/140‑1 (MQ=255)
                                                                                         ctctTCCTGCGTCTCTTTCTCCGGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTGCCCTCCTCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:441236/140‑1 (MQ=255)
                                                                                                 gCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcgcg                                                >  2:4339163/1‑134 (MQ=255)
                                                                                                  cTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCg                                                                                                  <  1:251036/83‑1 (MQ=255)
                                                                                                   ttCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCgg                                        <  2:47614/140‑1 (MQ=255)
                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:3155985/140‑1 (MQ=255)
                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       >  2:2013507/1‑140 (MQ=255)
                                                                                                    tctcTGTCTCCTGCGTGCGATATTTGTTTACTCCCGCGCTTCCCATCATTTTTTCCATCAGCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:1741872/140‑1 (MQ=255)
                                                                                                      tctTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     >  1:1294580/1‑108 (MQ=255)
                                                                                                         ttCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATc                                  <  2:1084297/140‑1 (MQ=255)
                                                                                                          tctcCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATcc                                 >  2:1683145/1‑140 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTACTCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  1:4014336/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  1:5051000/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  1:1218052/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:4170833/140‑1 (MQ=255)
                                                                                                                 cTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacga                          <  2:576930/140‑1 (MQ=255)
                                                                                                                 cTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacga                          >  1:1685992/1‑140 (MQ=255)
                                                                                                                         atTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacac                  <  2:3174189/140‑1 (MQ=255)
                                                                                                                         atTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacac                  <  1:2751598/140‑1 (MQ=255)
                                                                                                                         atTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacac                  <  2:4001879/140‑1 (MQ=255)
                                                                                                                          tttGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                 <  1:3910706/140‑1 (MQ=255)
                                                                                                                               ttAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGaa            <  1:2339119/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATGTTTTCCATCATCAGCTGGTCGGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:4161695/140‑1 (MQ=255)
                                                                                                                                    cccGCGCGTCCGATCATTTTTTTCATCATCAGCTGGTGTGTGGATGGCGGTGCTGCAGGACTCGGCGTGCTTGTCGGCTGTATGCGGGGCGCGCTCGCGGTGTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:4681717/140‑1 (MQ=255)
                                                                                                                                       gcgcGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAc    <  1:4341826/140‑1 (MQ=255)
                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:4540094/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: