Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 452,125 T→A 42.7% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA42.7% 213.0 / inf 339K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (88/105);  new base A (73/72);  total (162/177)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAC  >  NZ_CP034943/451988‑452263
                                                                                                                                         |                                                                                                                                          
      ccTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATa                                                                                                                                    >  2:4424389/1‑140 (MQ=255)
       cTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATaa                                                                                                                                   >  1:4243282/1‑140 (MQ=255)
        tCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                  >  1:4274029/1‑140 (MQ=255)
             tttGATGATTGAAAGCGTTTTGAAGAAGATTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCg                                                                                                                             <  1:2488071/140‑1 (MQ=255)
              ttGATGATTGACAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGc                                                                                                                            <  2:5132250/140‑1 (MQ=255)
                          tgctttttGAATACGTGTGGCCTTCAAAAACAAACCGAGTCGGGATTGGCAAAGCAATGAAGCGCATCAAATTTATAAATCTCCAAATGGGGTTGCTGTTGTTCCCTCAGTTTTTTGATAACAACCGCAACATCAAacga                                                                                                                <  1:1789598/136‑1 (MQ=255)
                          aGCGTTTTGAATAAGAGTGGCCTGTCAAAATAACCCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:4447138/140‑1 (MQ=255)
                          aGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:3163166/140‑1 (MQ=255)
                              ttttGAACAAGCGTGGCCTGTAAAAAAAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGt                                                                                                            <  1:1947551/140‑1 (MQ=255)
                              ttttCAAGAAGAGTGGCCTGTAAAAAAAAACCGACTCGGGATTGGCAAAGCCATGACGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGt                                                                                                            <  1:1288319/140‑1 (MQ=255)
                               tttGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGtt                                                                                                           <  2:1952385/140‑1 (MQ=255)
                                 tGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCa                                                                                                         >  2:3512665/1‑140 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                    >  1:2921973/1‑140 (MQ=255)
                                          gTGGCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAggg                                                                                                <  1:3997232/140‑1 (MQ=255)
                                          gTGGCATGAAAAAATAACCCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAggg                                                                                                <  1:26753/140‑1 (MQ=255)
                                           tGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGGTCATATGAAGGGa                                                                                               >  2:1538573/1‑140 (MQ=255)
                                           tGGCCTGTCAAAATAAACCGAATCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAATAACCGCAACATCAAACTACGGTTCATATGAAggta                                                                                               >  1:4139553/1‑138 (MQ=255)
                                             gctgctaaaacTAAACCGACTCGGGATTGGCAAACCAATGAAGAGCATCAAATTGATAAAACTCCAACTTGGGTTGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCACCATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:1895544/133‑1 (MQ=255)
                                             gccttacAAAATAAACCGAATCGGGATTGGAAAAGCCATGAAGCGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGACCCGCAACAACAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:4883725/134‑1 (MQ=255)
                                             gcatttaaaaaTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:4196011/133‑1 (MQ=255)
                                             gCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:5006497/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             >  1:439220/1‑140 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATAGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:3611634/140‑1 (MQ=255)
                                             gCCTGTCAAAACAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:522596/140‑1 (MQ=255)
                                             gCCTGTCAAAAAAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCAACAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:815436/140‑1 (MQ=255)
                                             gCCTGTCAAAAAAAACCGACTCGGGATTGCCAAAGCCATGAAGAGCATCAAAGTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:2969768/140‑1 (MQ=255)
                                             gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:3262544/140‑1 (MQ=255)
                                             gCCTGAAAAACTAAACCGACTCGGTAATGCCAAAGCAATGAAGAGCATCAAATTTATAAATCTCCAAATTGGGATGCTTTTGTTCCCTCAGTATTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:3080529/140‑1 (MQ=255)
                                             gCCTCTAAAAATAAACCGACACGGGATTGGAAAAGCAATGAAGAGCATCAAATTCATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAACAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:2828217/140‑1 (MQ=255)
                                             cccTGACAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:4506830/139‑1 (MQ=255)
                                                  tCAAAATACACCGACTCGGGATTGGCAAAGCCATGAAGAGAATCAAATTGATAAATCTCCAAATTGCGATGCTGATGTTCCCTCAGTTTATTGATAATAACCGCAACATCAAACGACGGTTAATATGAAGGGAAcaacaa                                                                                        >  1:2272414/1‑140 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATTAAGAGCCTCAAATTGATAACTCTCCAAATTGGGATTCTGTTGTTCCCTCAGTATTTTGATAAGAACCGAAACATAAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        <  1:2445882/140‑1 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCCGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        <  1:4995853/140‑1 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        >  2:1280512/1‑140 (MQ=255)
                                                   cAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACact                                                                                       >  2:4099565/1‑138 (MQ=255)
                                                       aTAAACCGCGTGGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGTATGCTTTTGTTCACTCAGTTTTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                   <  1:333480/140‑1 (MQ=255)
                                                       aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                   >  2:458585/1‑140 (MQ=255)
                                                            ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                              <  1:1093828/140‑1 (MQ=255)
                                                               aCTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAg                                                                           >  2:1741568/1‑140 (MQ=255)
                                                               aCTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAg                                                                           >  2:4379050/1‑140 (MQ=255)
                                                                cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACAGGGCAGa                                                                          >  2:2078137/1‑140 (MQ=255)
                                                                  cGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACt                                                                        <  2:185204/140‑1 (MQ=255)
                                                                         ggCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGt                                                                 >  1:5142357/1‑140 (MQ=255)
                                                                          gCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                >  1:989951/1‑140 (MQ=255)
                                                                               gcattGAAGAGCATCAAATTGATAAATGTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           <  2:2272414/136‑1 (MQ=255)
                                                                               gcaaTGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGATGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           <  2:67469/137‑1 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAATAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:2497015/1‑140 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACACTAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  1:1471198/1‑140 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:2377621/1‑140 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  1:3596223/1‑140 (MQ=255)
                                                                                       gagCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcg                                                   <  1:1383543/140‑1 (MQ=255)
                                                                                         tcATCAAATTGATAAATCTCCAAATGGGGCTGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                                 <  1:310292/139‑1 (MQ=255)
                                                                                          cATCAATTTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTAGTTTGAGAAGAACCGCAACATCAAACGACGGTGCCTATGCAGGGAAAAACAATTGTACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:4425641/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:2540592/76‑1 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:4709239/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:4481770/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:3458583/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  2:759191/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCCACATCAAacga                                                                                                                <  1:3127343/76‑1 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCCACATCAAacga                                                                                                                >  2:3127343/1‑76 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAggg                                                                                                >  2:3187883/1‑92 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:2112039/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:3740039/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:3868648/1‑140 (MQ=255)
                                                                                             cAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATc                                             >  1:2993335/1‑140 (MQ=255)
                                                                                                 ttGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                         <  2:114753/140‑1 (MQ=255)
                                                                                                 ttGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                         <  2:1377390/140‑1 (MQ=255)
                                                                                                    aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGACTCGCGTATCCGGGAAc                                      >  1:4594782/1‑140 (MQ=255)
                                                                                                    aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  2:2598602/1‑140 (MQ=255)
                                                                                                    aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  1:2523534/1‑140 (MQ=255)
                                                                                                      aaaTCTCCAAATTGGTATCCTGTTGTTCCCTAATTATTTTTATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                    <  2:4262687/140‑1 (MQ=255)
                                                                                                        aTCTCCAAATTGGTATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGta                                  <  1:2215018/140‑1 (MQ=255)
                                                                                                           tcCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGtaata                               <  1:2377621/140‑1 (MQ=255)
                                                                                                           gccAAATTGGTTTGCTGTTTTTCCATCATTATTTTTATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGTAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGtaata                               <  2:86919/139‑1 (MQ=255)
                                                                                                             cAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaac                             >  1:62199/1‑140 (MQ=255)
                                                                                                                aTTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAACACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaacaaa                          <  1:1146085/140‑1 (MQ=255)
                                                                                                                   gggATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAg                       <  1:187183/140‑1 (MQ=255)
                                                                                                                   gggATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAg                       <  1:5117999/140‑1 (MQ=255)
                                                                                                                     gATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                     >  2:2479537/1‑140 (MQ=255)
                                                                                                                      aTGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAgg                                                                                    >  1:1154159/1‑76 (MQ=255)
                                                                                                                         cTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                >  2:3839976/1‑93 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  2:4801260/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  2:4243282/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  2:4000898/140‑1 (MQ=255)
                                                                                                                            ttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCa              >  2:2176741/1‑140 (MQ=255)
                                                                                                                             tgttCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc             <  1:3512665/140‑1 (MQ=255)
                                                                                                                                tCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc          <  1:1679582/140‑1 (MQ=255)
                                                                                                                                tCCCTCAGTTTTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACAGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAAGAACAAACAGCTCCGCTCACCTc          <  1:2555308/140‑1 (MQ=255)
                                                                                                                                 cccTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa         >  1:3253710/1‑140 (MQ=255)
                                                                                                                                 cccTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa         >  2:3467638/1‑140 (MQ=255)
                                                                                                                                 cccTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa         >  2:145085/1‑140 (MQ=255)
                                                                                                                                  ccTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAg        <  2:3370132/140‑1 (MQ=255)
                                                                                                                                  ccTCAGTTTTTTGATAAGAACCGCAAAATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAg        <  2:4687321/140‑1 (MQ=255)
                                                                                                                                    tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTCATAACAAACAGCTCCGCTCACCTCAGGc      >  1:3308758/1‑140 (MQ=255)
                                                                                                                                    tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  1:695165/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGACCCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  2:2709510/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt    >  1:150763/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt    >  1:1007617/1‑140 (MQ=255)
                                                                                                                                        ttttttGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAc  <  1:659505/140‑1 (MQ=255)
                                                                                                                                        ttttttGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAc  <  1:2845098/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAC  >  NZ_CP034943/451988‑452263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: