Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,721,872 T→G 5.0% E156D (GAA→GAC EO946_RS19155 ← uracil‑DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,721,8720TG 5.0% 996.4 / 3.0 418E156D (GAA→GACEO946_RS19155uracil‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base T (159/216);  new base G (2/41);  total (161/257)
Fisher's exact test for biased strand distribution p-value = 1.85e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

CGGCCTGCTCCCGAAAAATCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGCCCAGCTGACA  >  NZ_CP034943/3721734‑3722007
                                                                                                                                          |                                                                                                                                       
          ccGAAAAATCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCACCCCCACAAAATAAAGACAACCGGCCGTTCTCTttcatt                                                                                                                              <  2:4100742/140‑1 (MQ=255)
             aaaaaTCCGGTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAg                                                                                                                           <  2:4128264/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGCGCATGCCGCCCCCACAAAATAAAGCCAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  2:2001161/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  1:615739/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  1:640569/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  2:3388975/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  1:1851452/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  1:1623088/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAAc                                                                                                                        <  1:2006543/140‑1 (MQ=255)
                aaTCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAAc                                                                                                                        <  1:3057553/140‑1 (MQ=255)
                aaTCCGTTTCTGTCAGAGAACGGGCTGGGTTGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  2:2986171/140‑1 (MQ=255)
                aaTCCGGTTCGTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGACCCCACAACATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        <  2:4478745/140‑1 (MQ=255)
                           tGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAAccc                                                                                                             <  1:95427/140‑1 (MQ=255)
                                agaACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTcc                                                                                                        <  2:4716927/140‑1 (MQ=255)
                                  aaCGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGt                                                                                                      >  2:2459704/1‑140 (MQ=255)
                                  aaCGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGt                                                                                                      >  1:3817886/1‑140 (MQ=255)
                                   aCGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTc                                                                                                     >  1:1689861/1‑140 (MQ=255)
                                      ggCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGa                                                                                                  <  2:4973001/140‑1 (MQ=255)
                                      ggCTGGGATGCGTGGATTCAATGAGAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGa                                                                                                  <  1:2806255/140‑1 (MQ=255)
                                       gCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGAt                                                                                                 >  2:3897096/1‑140 (MQ=255)
                                            gATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTcc                                                                                            >  2:4288504/1‑140 (MQ=255)
                                            gATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTcc                                                                                            >  2:210644/1‑140 (MQ=255)
                                            gATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTcc                                                                                            >  1:2461632/1‑140 (MQ=255)
                                            gATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTcc                                                                                            >  1:3524630/1‑140 (MQ=255)
                                            gATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTcc                                                                                            >  1:1076121/1‑140 (MQ=255)
                                             aTGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTccc                                                                                           <  1:2160370/140‑1 (MQ=255)
                                             aTGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTccc                                                                                           <  1:4723190/140‑1 (MQ=255)
                                             aTGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTccc                                                                                           <  1:691595/140‑1 (MQ=255)
                                              tGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCa                                                                                          <  1:4322150/140‑1 (MQ=255)
                                              tGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCa                                                                                          <  1:177230/140‑1 (MQ=255)
                                                cGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGTACGTCAATAACCCTGTCCGTCAGATGCTCCCAGc                                                                                        >  2:186304/1‑140 (MQ=255)
                                                cGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGc                                                                                        >  1:5152555/1‑140 (MQ=255)
                                                 gTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTctct                                                                                                                                    <  1:3333990/95‑1 (MQ=255)
                                                 gTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGa                                                                                                  <  1:2306001/129‑1 (MQ=255)
                                                 gTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGcc                                                                                       >  2:3184953/1‑140 (MQ=255)
                                                 gTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCACCCACAAAATAAAGACAACCGGCCGTTATATTTCATTCTGAACGTCAATAAACCTTTCCGTCAGATGCTccacgac                                                                                       >  2:3258753/1‑135 (MQ=255)
                                                  tGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAACTAAAGACAACCGGCCGTTCTCTTTCTGTCAGCAGGTCACTAACCCGGTCCGTCAGGTGCGCGCAGccg                                                                                      >  2:2923294/1‑139 (MQ=255)
                                                  tGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAAACCTGTCCGTCAGATGCTCCCAGCCt                                                                                      >  2:5178519/1‑140 (MQ=255)
                                                   ggATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCtt                                                                                     <  1:4599921/140‑1 (MQ=255)
                                                   ggATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCtt                                                                                     <  1:1743346/140‑1 (MQ=255)
                                                   ggATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCtt                                                                                     <  1:1931977/140‑1 (MQ=255)
                                                     aTTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTATCTTTCATTCATAACGTCAATAAACCTGTCCGTCAGATGCTCCCACCCtatt                                                                                   >  1:1340142/1‑140 (MQ=255)
                                                        cAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTccc                                                                                <  2:84325/140‑1 (MQ=255)
                                                        cAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTccc                                                                                <  2:3736546/140‑1 (MQ=255)
                                                        cAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTccc                                                                                >  2:895439/1‑140 (MQ=255)
                                                         aaTGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCGCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCt                                                                               >  2:5048409/1‑140 (MQ=255)
                                                            gATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCCCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTAt                                                                            <  2:2173178/140‑1 (MQ=255)
                                                               aaaaTGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATggg                                                                         >  2:3511134/1‑140 (MQ=255)
                                                               aaaaTGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATggg                                                                         >  2:815877/1‑140 (MQ=255)
                                                                 aaTGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCt                                                                                                            >  1:4977179/1‑103 (MQ=255)
                                                                   tGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAAtt                                                                     >  2:1745676/1‑140 (MQ=255)
                                                                      ttGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAACGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGc                                                                  <  1:3976522/140‑1 (MQ=255)
                                                                       tGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGGCAAAAACCCAGTCCGTCAGATGCTCCCACCCTTTTCACGTATGGGAATTAGcc                                                                 >  1:4544172/1‑140 (MQ=255)
                                                                          aGGTGTCGATCCGTTGTTTTTTCATTTGAGCATGCCGCCCCCCCAAAATATAGACAACCGGCCGTTCTCTTTCAGTCAGCACGTCAATACCCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGt                                                              <  1:1012369/140‑1 (MQ=255)
                                                                          aGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGt                                                              <  1:1132524/140‑1 (MQ=255)
                                                                            gtgtCGATCCGTTATTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTcc                                                            <  2:4066452/140‑1 (MQ=255)
                                                                              gtCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTAATTCAGAACGTCAATAACCCTGTACGTCAGATGCTCCCAGCCATTTCCCGTATGGGAATTCGCCAGTCcgc                                                          >  1:4638343/1‑140 (MQ=255)
                                                                               tCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAAc                                                                                                                        >  1:3456656/1‑77 (MQ=255)
                                                                               tCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCcgcg                                                         >  2:2627932/1‑140 (MQ=255)
                                                                                   tCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCtt                                                     <  2:1302308/140‑1 (MQ=255)
                                                                                   tCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCtt                                                     <  2:4783046/140‑1 (MQ=255)
                                                                                     cGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTAc                                                   <  1:3996509/140‑1 (MQ=255)
                                                                                     cGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCAGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTAc                                                   <  1:2703478/140‑1 (MQ=255)
                                                                                       ttCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTg                                                 >  2:4177278/1‑140 (MQ=255)
                                                                                        tCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATCCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGt                                                <  2:1707704/140‑1 (MQ=255)
                                                                                            ttttCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGc                                            <  1:791074/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATACAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  1:3676556/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATACAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  2:3442201/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  1:4831593/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  1:3298397/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  2:687763/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  2:5112949/140‑1 (MQ=255)
                                                                                             tttCATTTGAGCATGCCGCCCCCACAAAATAAACACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGca                                           <  2:163761/140‑1 (MQ=255)
                                                                                               tCATTTGAGCATGCCGCCCCCACAAAATAACGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGcaca                                         <  2:4849985/140‑1 (MQ=255)
                                                                                               tCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGcaca                                         <  1:1423919/140‑1 (MQ=255)
                                                                                               tCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGcaca                                         <  1:258874/140‑1 (MQ=255)
                                                                                                cATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAg                                        <  2:2405188/140‑1 (MQ=255)
                                                                                                     gAGCATGCCGCCCCCACAAACTAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTc                                   <  2:4638343/140‑1 (MQ=255)
                                                                                                     gAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTc                                   <  1:841829/140‑1 (MQ=255)
                                                                                                       gCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAg                                 <  1:3731279/140‑1 (MQ=255)
                                                                                                           gccgccCCCACAAAATAAAGACAACCGGCCGTTCTCTATAATTCAGAAACTCAATAAACCTGTCCGTCAGAAGCTCCCACCCTTTTCCCTTATGGGAAATAGCCTGTCCGCGCCTTACTGTCAGCACAGAGTTAAGAAAt                             >  1:4176675/1‑140 (MQ=255)
                                                                                                            ccgccCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATa                            <  2:1588184/140‑1 (MQ=255)
                                                                                                             cgccCCCACCAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAAGAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAg                           <  2:2529668/140‑1 (MQ=255)
                                                                                                              gccCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGa                          <  2:1065670/140‑1 (MQ=255)
                                                                                                              gccCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGa                          <  2:1179922/140‑1 (MQ=255)
                                                                                                              gccCCCACAAAATAAAGACAACCGGCCGGTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCCGCAATAGa                          <  2:2021560/140‑1 (MQ=255)
                                                                                                                ccccACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGc                                                                  <  2:2298267/98‑1 (MQ=255)
                                                                                                                ccccACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAAc                        >  2:3649749/1‑140 (MQ=255)
                                                                                                                ccccACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAAc                        >  2:3589534/1‑140 (MQ=255)
                                                                                                                   cacaAAATACAGACAACCGGCCGGTCTCTGTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAAcccc                     <  1:2930345/140‑1 (MQ=255)
                                                                                                                   cacaAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAAcccc                     <  1:4079625/140‑1 (MQ=255)
                                                                                                                     caAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTAAGCACAGTGTTCAGCAATAGAACCCCtt                   >  2:3921488/1‑140 (MQ=255)
                                                                                                                        aaTAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCt                <  1:3282239/140‑1 (MQ=255)
                                                                                                                           aacgccAACCGGCCGGTCTCTTTCCTTCAGAACGACAATACCCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGCGAATTCGCCTGTCCGCGCCGTACTGTCAGCACAGTGTTCAGCAATAGACCCCCTTGCTTTg             <  1:2080520/135‑1 (MQ=255)
                                                                                                                             aGACAACCGGCCGTTCTCTTTCATTCAGAACGTCACTACCCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGcc           <  1:4238994/140‑1 (MQ=255)
                                                                                                                             aGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCGTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGcc           <  1:4115947/140‑1 (MQ=255)
                                                                                                                                cAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGCCCAg        <  2:637116/140‑1 (MQ=255)
                                                                                                                                cAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGCCCAg        >  2:1477513/1‑140 (MQ=255)
                                                                                                                                  aCCGGCCGTTCTCTTTCATTGAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCCGCCTTTTCCCGTATGGGAATTCGCCTGTGCGCGCCTTACTGTCAGCACAGGGGTCAGCAATAGAACCCCTTGCTTTGCCCCGCt      >  1:1203547/1‑140 (MQ=255)
                                                                                                                                  aCCGGCCGTTCTCTTTCATTCAGACCGTCAATAACCCTGTCCGTCAGATTCTCCCACCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGACACCCTTGGTGTGCCCAGCt      >  2:2828108/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CGGCCTGCTCCCGAAAAATCCGTTTCTTGCAGAGAACGGGCTGGGATGCGTGGATTCAATGATAAAATGTTTGGAGGTGTCGATCCGTTCTTTTTTCATTTGAGCATGCCGCCCCCACAAAATAAAGACAACCGGCCGTTCTCTTTCATTCAGAACGTCAATAACCCTGTCCGTCAGATGCTCCCAGCCTTTTCCCTTATGGGAATTCGCCTGTCCGCGCCTTACTGTCAGCACAGTGTTCAGCAATAGAACCCCTTGCTTTGCCCAGCTGACA  >  NZ_CP034943/3721734‑3722007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: