BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,893	T→G	6.4%	N59H (AAC→CAC)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,893	0	T	G	6.4%	822.1 / 10.4	314	N59H (AAC→CAC)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base T (83/199); new base G (30/2); total (113/201)
Fisher's exact test for biased strand distribution p-value = 7.45e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

TTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAA  >  NZ_CP034943/3746755‑3747031

tttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGttt                                                                                                                                           >  2:3114906/1‑140 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                                                          >  2:430646/1‑139 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                                                          >  2:2799979/1‑139 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                          >  1:1068587/1‑140 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                          >  2:733793/1‑140 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                          >  2:4756928/1‑140 (MQ=255)

         aTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCa                                                                                                                                  >  2:3413546/1‑140 (MQ=255)

         aTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCa                                                                                                                                  >  2:2775823/1‑140 (MQ=255)

             aTAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcag                                                                                                                              <  1:3110269/140‑1 (MQ=255)

             aTAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcag                                                                                                                              <  1:4756928/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:4090716/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             >  2:2416839/1‑140 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  1:3248114/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:2027617/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:3563907/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:1298357/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:3836768/140‑1 (MQ=255)

               aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                                            <  2:2309903/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:224034/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:118677/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACCTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:2668361/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCACCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:1923142/140‑1 (MQ=255)

                    aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAACGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                                       <  2:3681252/140‑1 (MQ=255)

                    aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                                       <  2:1114169/140‑1 (MQ=255)

                     cGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCatat                                                                                                                      >  2:2909453/1‑140 (MQ=255)

                      gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                                     <  1:1481498/140‑1 (MQ=255)

                       cgtttCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTTTAGTTGTGCAACACCAGCAAATGt                                                                                                                    >  1:2954981/3‑140 (MQ=255)

                          ttCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTAcc                                                                                                                 <  2:3729128/140‑1 (MQ=255)

                           tCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAACAGCAGCATATGTAacc                                                                                                                >  2:3348243/1‑140 (MQ=255)

                                   ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCACCAAATTTACCCATTGTAAg                                                                                                        >  1:2875504/1‑140 (MQ=255)

                                   ttCGCCCAGTCGTCTTCTTGTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTTTTTATGCAACAGCAGCATATGTACGCAATGTCAg                                                                                                        >  2:1713891/1‑140 (MQ=255)

                                       cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGTCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                                                                    <  1:4475650/140‑1 (MQ=255)

                                       cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGGGTTTATGCAACAGCACCCAATTTACCCAGTGTCAgcgcg                                                                                                    >  1:2085432/1‑140 (MQ=255)

                                         cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCa                                                                                                  <  1:3674288/140‑1 (MQ=255)

                                            tcgtcTTCTTTTTGATAGCGGATGTCGTATAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:2314375/112‑1 (MQ=255)

                                             cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                                                              <  2:192989/140‑1 (MQ=255)

                                             cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                                                              <  1:639156/140‑1 (MQ=255)

                                                 ttcttTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTATGCAAAAACAGCATATGTAACCATTTTAAGCGCCCAAGGaaaaa                                                                                          >  2:4142568/1‑138 (MQ=255)

                                                       ttGATAGCGGCTGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  2:841400/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:1995143/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:4802518/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:2596943/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:2446621/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:235475/140‑1 (MQ=255)

                                                         gagaGCGGATGTCGTCTAGGCTGCATTCCTTGCGCCCGCCCTCTGTACAGTTGTCGGAATACCTCCGGACAACCCCGGCGTGTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:256545/137‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:2539207/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:713782/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:1127066/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:3959243/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:4095240/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGGCGTGTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:873014/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:3280874/140‑1 (MQ=255)

                                                         gATAGCGGAGGTCGTCTAGGCTGCATTCCTGGATCCCTCCGTCTTTAAAGTGGTCTGACTACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:3736874/140‑1 (MQ=255)

                                                              cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACACCACCAAAAGTACCCAATGTAAGCCCGCCCGGAAACACAAGcccccccat                                                                             >  2:68057/1‑137 (MQ=255)

                                                               ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                                            <  2:3914075/140‑1 (MQ=255)

                                                                gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                           <  2:4674459/140‑1 (MQ=255)

                                                                gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                           <  1:220338/140‑1 (MQ=255)

                                                                gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                           <  2:588266/140‑1 (MQ=255)

                                                                gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                           <  1:4718525/140‑1 (MQ=255)

                                                                 atttcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtata                                                                          <  2:2708925/137‑1 (MQ=255)

                                                                 aTGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtata                                                                          <  2:4808955/140‑1 (MQ=255)

                                                                  tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                                                                         <  1:3122688/140‑1 (MQ=255)

                                                                    tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                       <  1:501481/140‑1 (MQ=255)

                                                                    tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                       <  2:1239623/140‑1 (MQ=255)

                                                                    tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                       <  2:4864871/140‑1 (MQ=255)

                                                                         tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                  <  2:2633805/140‑1 (MQ=255)

                                                                         tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                  <  2:482242/140‑1 (MQ=255)

                                                                         tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                  <  1:770538/140‑1 (MQ=255)

                                                                         tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                  <  1:985119/140‑1 (MQ=255)

                                                                         tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGGACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                  <  2:3575045/140‑1 (MQ=255)

                                                                           ggCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTGTGCAAAAACAGCAAATTTAACCAATTTAACCGCGCCCCGAAAAACAAACCCCCCCGTATAATatgtatgt                                                                >  1:1640027/1‑140 (MQ=255)

                                                                            gCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGtt                                                               <  1:4849340/140‑1 (MQ=255)

                                                                               gCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTtctc                                                            <  2:1603828/140‑1 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACACCAGCATATTTAACCATTGTCAGCGCGCACGGAAACAAATGCCCCCCGGTATAATATTTAttttatac                                                           >  2:4425713/1‑136 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATTTAACCATTGTGAGCGCGCACGGAAACACATGACCGCCGTTATAATCTGTATGTTCTcc                                                           >  2:1440919/1‑140 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATTCAAAACCACCATAAGTACACAATGTCACAGCGCACGGAAAAACAAGCCCGCCGTTATAATATGTATGTtgtca                                                           >  2:1076619/1‑139 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCATATTTACCCATTGTCAGCCCGCACGGAAACACATTCCCCCCGGTATAATATGTATGttatac                                                           >  2:2363228/1‑136 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTATTGAACAGCAGCATATTTACCCATTTTCAGCGCGCACGGAAAAAAATGCCCGCCGGTATAATATGTATGTTATcc                                                           >  2:2322963/1‑140 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTTCAAAAACAGCAAATGTAACCCTTGTCAGCGCGCCCCGAAACACAAGCCCCCCGGTAAAAACTGTATTTTCTcc                                                           >  1:1225038/1‑140 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGGTGTTGTGCCAACGCAGCAAAAGTAACCCTTGTCATCCCGCACCGAAACACAGTCCCGCCCGTATAAACGGTGTGttgtac                                                           >  1:2290670/1‑136 (MQ=255)

                                                                                 aTTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCa                                                          <  2:919459/140‑1 (MQ=255)

                                                                                 aTTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCa                                                          <  1:382720/140‑1 (MQ=255)

                                                                                 aTTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAACAACAGCAAATGTAAACAAAGTCAGCCCGCACCGAAAAACATACCCCCCGTTATAATCTTTATTTTATCCa                                                          >  1:3555940/1‑140 (MQ=255)

                                                                                   tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                        <  2:4115349/140‑1 (MQ=255)

                                                                                   tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                        <  1:4592637/140‑1 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACACCCCCGTCGTTTCTGTCTGCAACAGCAGCATATGTACCCATTGTAAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                 <  2:1515339/140‑1 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGGAACATCACCATATGTAAACAATTTCACAGCGCCCCGCAAAACCAGGCCGCCGGAATAATAGGTGTGTTGTCCACTCAgtatc                                                 >  1:4395397/1‑136 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                 <  2:2197387/140‑1 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                 <  2:141248/140‑1 (MQ=255)

                                                                                                cGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAAAACCAGCCAATGTAACCAATGTCAGCGCGCACCGAAACAAAATCCCGCCGGTAAAATAAGTATGTTATACAATAAGAtttaaaatt                                           >  1:5161893/1‑133 (MQ=255)

                                                                                                      tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCAGCATATTTACCCAGTGTCAGCGCGCCCGGAAACAAATGCCCGCCGGTATAATATGTATGTTGTCCCATCAGATTCACTATTACAggg                                     >  1:5060971/1‑139 (MQ=255)

                                                                                                                     aCATCCGGACAACCCCGTCGTTTGTTTATGCAACAGCAGCATATGTACCCCTGGGCAGCGCGCACGGAAAAACAAGCCCGCCGTTAAAATCTGTATGTTGTCCCAACAGATTAACACTTACAGGGTCGAAAAAAAACCGt                      >  2:5044/1‑140 (MQ=255)

                                                                                                                        tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                   <  1:2775823/140‑1 (MQ=255)

                                                                                                                        tCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                   <  1:3413546/140‑1 (MQ=255)

                                                                                                                          cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc                 <  1:1415116/140‑1 (MQ=255)

                                                                                                                          cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc                 <  2:4508515/140‑1 (MQ=255)

                                                                                                                                 ccccGTCGTTTCTTTCTGCAACAACAGCAAATGTACCCATTGTCACCGCGCCCGGAAACACAAGCCCCCCCTTATAAACTGTATTTTCTACAATCAGATTCAAAATTACAGGTGGGTAAAAAAAACGGAACACCTCgttt          >  2:1843013/1‑140 (MQ=255)

                                                                                                                                  cccGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCttttt         <  1:430646/140‑1 (MQ=255)

                                                                                                                                  cccGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCttttt         <  1:2799979/140‑1 (MQ=255)

                                                                                                                                    cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct       <  2:4201139/140‑1 (MQ=255)

TTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAA  >  NZ_CP034943/3746755‑3747031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`