Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,846,645 C→A 6.1% D188Y (GAT→TAT)  dbpA ← ATP‑dependent RNA helicase DbpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,846,6450CA 6.1% 885.0 / 14.4 312D188Y (GAT→TAT) dbpAATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base C (126/158);  new base A (25/2);  total (152/160)
Fisher's exact test for biased strand distribution p-value = 7.05e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

ACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGACGGTCTAAC  >  NZ_CP034943/3846506‑3846784
                                                                                                                                           |                                                                                                                                           
acaTCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTTACGGCTCAATTTCTCAATAtc                                                                                                                                             >  1:648649/1‑140 (MQ=255)
    cTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATAtcctcc                                                                                                                                         >  1:3668542/1‑140 (MQ=255)
    cTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATAtcctcc                                                                                                                                         >  1:2577267/1‑140 (MQ=255)
           cAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCg                                                                                                                                  <  1:4108494/140‑1 (MQ=255)
              aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTTATTTCAATATTCTGCGGGTTTTGCATATATTGACGGCGCAATTTCTCAATATCCTCCGGCAGCGTTg                                                                                                                               >  1:1550502/1‑140 (MQ=255)
              aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTTATTTCAATATGCTTCGGGTTTTGCATATATTGACGGCTCAATTTGTCAATATGCTCCGGCAGCGTTg                                                                                                                               >  1:456804/1‑140 (MQ=255)
              aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTTATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTg                                                                                                                               >  2:3385321/1‑140 (MQ=255)
              aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTg                                                                                                                               >  2:906249/1‑140 (MQ=255)
                   aCTTATTGTGTTCTCTTACTTGAATCACCGCATGTTCAATATTGTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaa                                                                                                                          <  1:5115595/140‑1 (MQ=255)
                   aCTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaa                                                                                                                          <  1:3770911/140‑1 (MQ=255)
                     ttATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaaaa                                                                                                                        <  2:3214911/140‑1 (MQ=255)
                                  ttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGGTCAATTTATCCATATACTCCGGCAGCGTTGCCGAAAACAACAACGTCGTg                                                                                                           >  1:147510/1‑140 (MQ=255)
                                  ttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCGTTGATTTAAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGGTGCCGAAAACAAAATAGTCGTg                                                                                                           >  1:339920/1‑140 (MQ=255)
                                    aCTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATTCTGCGGGTTTTGCATATATTTACGGCTCAAATTCTCAATATACTCCGGCAGCGTTTCCGAAAACAACATCGTCGTgcg                                                                                                         >  1:1203076/1‑140 (MQ=255)
                                     cTTGAATCACCGCATGTTCAATATTCGTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTTACGGCTCAATTTCTCAATATCCTCCGGCAGCGATGCCGAAAAAAACATCGTCGTgcgc                                                                                                        >  2:2256186/1‑140 (MQ=255)
                                      ttGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGct                                                                                                       <  1:3581427/140‑1 (MQ=255)
                                         aaTCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTg                                                                                                    >  1:5080324/1‑140 (MQ=255)
                                          aTCACCGCATGTTCCATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGt                                                                                                   >  2:1398859/1‑140 (MQ=255)
                                              ccGCAGGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAACACAACCTCGGCGTGCGCTCTGTCGGc                                                                                               >  2:3112819/1‑140 (MQ=255)
                                               cGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTAAATATTCTGCGGGTTTTGCATATATTTACGGCTCAAATTCTCAATATACTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCa                                                                                              >  1:2934701/1‑140 (MQ=255)
                                                gCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAg                                                                                             <  2:3717476/140‑1 (MQ=255)
                                                 cATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGa                                                                                            <  1:1971138/140‑1 (MQ=255)
                                                   tGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAAATTATCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATg                                                                                          >  1:2089666/1‑140 (MQ=255)
                                                         atatTCTTTGTGGTTAGGCCGGCCGCTTTGATTTAAATATTCTGCGGGTTTTTGATATATTTACGGCTCAAATTCTAAATATCCTACGGCAGCGGTTCCGAAAACAACATCGTACTGCGCTCTGTAGGCAGATGCTTGAt                                                                                    >  1:3001980/1‑140 (MQ=255)
                                                         atatTCTTTGTGGTTAGGCCGGCCGCTTTGATTTAAAAATTCGGCGGGTTTTTCAAAAATTTAACGCTCAAATTATAAAAATCCTACGGCAACGTTGACGAAAACAACAACGTCGTGCGCTCTGTAGGCACAAGCTTTAt                                                                                    >  1:5019218/1‑140 (MQ=255)
                                                           atTCTTTGTGGTTAGGCCGGCCGCTTTGATTACAATATGCTGCGGGTTTTGCATATATTGACGGCTCACTTTCTCACTATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATaa                                                                                  <  2:5117388/140‑1 (MQ=255)
                                                            ttCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAAt                                                                                 <  2:346659/140‑1 (MQ=255)
                                                             tCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATc                                                                                <  1:1001750/140‑1 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTTATTTCAAAATTCTTCGGGGTTTGCAAATATTGAACGCTCAAATTATAAATATACTCCCGCAGCCTTGCCGAAAACAAAATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATAGc                                                                              >  1:2238393/1‑140 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCt                                                                                        <  1:1158590/130‑1 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                                              <  1:264872/140‑1 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                                              <  1:2346372/140‑1 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                                              <  1:1602816/140‑1 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                                              >  2:499829/1‑140 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATACTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCt                                                                                        >  2:1158590/1‑130 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTAAAAATGCTGCGGGTTTTTCAAATAATTACGGCTCAAATTATCAAAATAATCCGGCAGCGTTGCCGAAAACAAAATAGTCGTGCGCACTGTCCGCAAATGCTTGATAAACGc                                                                              >  1:2464261/1‑140 (MQ=255)
                                                               tttGTGGTTAGGCCGGCCGCTTTGATTTAAAAATGCTGCGGGTTTTGCATATATTTACGGCTCAAATTATCAAAATCCTCCGGCAGCGTTTGCGAAAAAAAAAAAGTCGTTCGCTCTGTCGGCAGATTCTTGATAATCGc                                                                              >  1:1818756/1‑140 (MQ=255)
                                                                ttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGcc                                                                             >  2:1966063/1‑140 (MQ=255)
                                                                 tgtgGTTAGGCCGGCCGCTTTGATTTCAAAATTATGCGGGTTTTGCATATATTTAAGGCTCAATTTATAAATATACTACGGCAGCGTTGCCGAAAACAAAAAACTCGTGCCCTATGTCGGCAGATTCTTGATAAACGcca                                                                            >  1:99462/1‑139 (MQ=255)
                                                                 tgtgGTTAGGCCGGCCGCTTTGATTTCAAAATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCGACGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCct                                                                            >  1:4517284/1‑140 (MQ=255)
                                                                        aGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCACATTCTTGATAATCGCCTCTACTTg                                                                     >  2:2700631/1‑140 (MQ=255)
                                                                             ggccgCTTTGATTTAAATATGCTGCGGGTTTTTCATATATTTACGGCTCAATTTATAAATATACTCCGGCAGCGTTTCCGAAAAAAACATAGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTTCTCGa                                                                >  1:2838841/1‑140 (MQ=255)
                                                                              gccgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGAt                                                               >  2:3612050/1‑140 (MQ=255)
                                                                              gccgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGATCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGGGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGAt                                                               <  1:1496/140‑1 (MQ=255)
                                                                               ccgCTTTGAGTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATg                                                              >  2:1504365/1‑140 (MQ=255)
                                                                                cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCTGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTTTAGTCAGTTGCTTTATAATCTACTCTACTTGCTCGAttt                                                             <  2:135932/140‑3 (MQ=255)
                                                                                cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                                             <  1:4260117/140‑1 (MQ=255)
                                                                                cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                                             <  2:2464261/140‑1 (MQ=255)
                                                                                cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                                             <  1:3175323/140‑1 (MQ=255)
                                                                                cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTGGCTCGATGa                                                             <  1:2217145/140‑1 (MQ=255)
                                                                                     tGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGccc                                                        <  1:5045954/140‑1 (MQ=255)
                                                                                        tttCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCata                                                     <  1:2717726/140‑1 (MQ=255)
                                                                                          tCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATAtt                                                   <  2:76206/140‑1 (MQ=255)
                                                                                             atatGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                                             >  1:20149/1‑127 (MQ=255)
                                                                                             atatGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                                             >  1:3078669/1‑127 (MQ=255)
                                                                                              tatGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGc                                               <  2:575209/140‑1 (MQ=255)
                                                                                                 gctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGc                                               >  2:3023198/1‑137 (MQ=255)
                                                                                                 gctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGc                                               >  2:4575840/1‑137 (MQ=255)
                                                                                                 gctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAtc                                            >  1:4881771/1‑140 (MQ=255)
                                                                                                  ctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAtct                                           >  2:2589810/1‑140 (MQ=255)
                                                                                                  ctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAtct                                           >  2:824399/1‑140 (MQ=255)
                                                                                                     cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgc                                                                                                          >  2:2852805/1‑74 (MQ=255)
                                                                                                     cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                                              <  1:3312410/102‑1 (MQ=255)
                                                                                                     cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCAt                                        >  2:1058299/1‑140 (MQ=255)
                                                                                                     cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCAt                                        >  2:478003/1‑140 (MQ=255)
                                                                                                      gggTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATc                                       <  1:3195963/140‑1 (MQ=255)
                                                                                                      gggTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATc                                       <  1:3042528/140‑1 (MQ=255)
                                                                                                           ttGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCtt                                  <  2:2934701/140‑1 (MQ=255)
                                                                                                               atatatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtcatc                              <  1:1687842/140‑1 (MQ=255)
                                                                                                                 atatTGACGGCTCAGCTTCTCGATATCCTGCGGAAGCGTCGCTGAAAACAGCATCGTCGTACGCTCAGTCGGCAGATGCTTGATAATCGCCTCCACCTGCTCAATGAAGCCCATATTCAGCATCTCATCCGCTTCATCaa                            >  2:4739730/1‑140 (MQ=255)
                                                                                                                  tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           >  2:3879179/1‑140 (MQ=255)
                                                                                                                  tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           <  2:5040978/140‑1 (MQ=255)
                                                                                                                  tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           <  2:2788273/140‑1 (MQ=255)
                                                                                                                  tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           <  2:3931301/140‑1 (MQ=255)
                                                                                                                  tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           >  1:1375857/1‑140 (MQ=255)
                                                                                                                    ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCTGCTTCATCAATGa                         <  1:2991977/140‑1 (MQ=255)
                                                                                                                    ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa                         <  1:1075959/140‑1 (MQ=255)
                                                                                                                    ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa                         <  2:4318761/140‑1 (MQ=255)
                                                                                                                    ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa                         <  1:1321841/140‑1 (MQ=255)
                                                                                                                        cGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCaa                     >  1:91478/1‑140 (MQ=255)
                                                                                                                        cGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGCCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCGTCATCAATGACCaa                     >  1:400012/1‑140 (MQ=255)
                                                                                                                         ggCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCaaa                    <  2:1585347/140‑1 (MQ=255)
                                                                                                                         ggCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCaaa                    <  2:147510/140‑1 (MQ=255)
                                                                                                                          gCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAAt                   <  1:3909683/140‑1 (MQ=255)
                                                                                                                           cTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATa                  >  2:4127297/1‑140 (MQ=255)
                                                                                                                            tCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAg                 >  2:3309839/1‑140 (MQ=255)
                                                                                                                            tCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAg                 >  2:1224203/1‑140 (MQ=255)
                                                                                                                                  tctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGgagaga           <  1:784244/140‑1 (MQ=255)
                                                                                                                                  tctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGgagaga           <  1:2803249/140‑1 (MQ=255)
                                                                                                                                  tctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGgagaga           <  1:4336502/140‑1 (MQ=255)
                                                                                                                                   ctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTa                                                                         >  2:2052047/1‑77 (MQ=255)
                                                                                                                                   ctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAAt                   <  2:3807749/131‑1 (MQ=255)
                                                                                                                                   ctcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGAc          <  2:4403109/140‑1 (MQ=255)
                                                                                                                                   ctcAATATCCTCAGGCAGGGTTGACGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGAc          <  1:3455913/140‑1 (MQ=255)
                                                                                                                                   cgcAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGGGCGCTCGGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAAGAGGAGAGAc          <  2:3441859/138‑1 (MQ=255)
                                                                                                                                     caagagcctccGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGACgg        <  1:1757317/134‑1 (MQ=255)
                                                                                                                                     cAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           <  1:4679989/121‑1 (MQ=255)
                                                                                                                                     cAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt                           >  2:4679989/1‑121 (MQ=255)
                                                                                                                                     cAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGACgg        <  2:648649/140‑1 (MQ=255)
                                                                                                                                     cAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGACgg        >  1:610987/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACCAAATAGGAGAGACGGTCTAAC  >  NZ_CP034943/3846506‑3846784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: