BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,220	G→T	9.3%	T52K (ACG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,220	0	G	T	9.3%	1127.0 / 39.1	413	T52K (ACG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (251/108); new base T (3/48); total (256/157)
Fisher's exact test for biased strand distribution p-value = 2.81e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTT  >  NZ_CP034943/3851083‑3851357

gAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGtt                                                                                                                                         >  1:1514825/1‑140 (MQ=255)

  cagagAACTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                       <  1:4784229/139‑1 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                       <  2:5016722/140‑1 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                       <  2:3189813/140‑1 (MQ=255)

     agaAGTCCTGCTATGTCCCAATCGGCTCTCTGTAATATGGAGTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                    <  1:3878004/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                  <  2:3292388/140‑1 (MQ=255)

         gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGACTTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                >  1:2667260/1‑140 (MQ=255)

                  tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                       <  2:36686/140‑1 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                     >  1:4128235/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                     >  1:2382066/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                     >  2:4313976/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGGCTTCTATAAATGTACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                     >  1:1885415/1‑140 (MQ=255)

                      ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                   <  2:2382066/140‑1 (MQ=255)

                       cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  <  1:805699/137‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  >  1:2743028/1‑140 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  <  1:3642649/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  <  1:668836/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATCTGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGGAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  2:4411783/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  2:1829411/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  2:3559154/140‑1 (MQ=255)

                         aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                >  2:2819624/1‑140 (MQ=255)

                         aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                >  2:2562581/1‑140 (MQ=255)

                         aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGt                                                                                                                >  2:2456382/1‑140 (MQ=255)

                             gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                            <  2:1002091/140‑1 (MQ=255)

                             gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                            <  2:1893745/140‑1 (MQ=255)

                             gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                            <  2:1497189/140‑1 (MQ=255)

                              ctctctGTAATATTGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAATTACTCGGACAGGTCTTCTATAAATTAACCATTCTTTATTACCTGTATAAAGATTCGATCGTATgaa                                                                                                           >  2:1269593/1‑138 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  2:3453231/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:1450031/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:1211670/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  2:4727446/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCa                                                                                                           >  1:2578107/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAACTTTATCAATATTCGATCGTATTCa                                                                                                           >  1:2458242/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAACTGTATCATGATTAGATCGTATTCa                                                                                                           >  1:3396101/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAAGCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTGGTGAGTCCCTGTCTCATGATTCGATCGTCTGCAAGccc                                                                                                      >  1:3063286/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATCATGATTCGATCGTATGCAAGccc                                                                                                      >  2:341245/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                      >  1:3329612/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTAGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATTAACCAGTCGTTATTACCTGTATCATTATTCGAAAGTTTGAAAGccc                                                                                                      >  1:4859463/1‑140 (MQ=255)

                                        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTccc                                                                                                 >  1:518424/1‑140 (MQ=255)

                                        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTccc                                                                                                 >  2:4704327/1‑140 (MQ=255)

                                           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              <  1:443966/140‑1 (MQ=255)

                                           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              <  1:2883700/140‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTAGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:1556155/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                       <  1:4563651/113‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                       <  2:2738588/113‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:1996651/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:5132688/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:2052245/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:4061338/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGCCCTTCCCaaaaa                                                                                            >  1:2056451/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                          >  1:3710500/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACg                                                                                          >  1:1805728/1‑140 (MQ=255)

                                                            ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGActct                                                                             >  2:2477376/1‑140 (MQ=255)

                                                                         ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGtt                                                                >  2:727243/1‑140 (MQ=255)

                                                                           ttATCACCTTGTAAGCTTAACCTTAAATGTAGGCGCACGGGTCTGCTATACATCAACCAGTCGTTTTTAGCGGTTTCATTATGCGAGCGTTGGCAAGCCGTACCAACAAACGATAAAACGACTCTGCTTCTTCAAGTTCg                                                              <  2:791718/140‑1 (MQ=255)

                                                                           ttAACACCTTGAAACAATAAACTTCAAAGTACTCGCACTGTTTTTATAAAAATCAACCAGTCTTTATAACCTTTATCTTTTTTCTATCTTATGAACCCCCTACCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCg                                                              <  2:518424/140‑1 (MQ=255)

                                                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                       >  2:3787999/1‑140 (MQ=255)

                                                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                       >  2:4871638/1‑140 (MQ=255)

                                                                                   ttttAAGCAGAACCTTCAAAGTACCCGCACAGGTCTTCTATAAATTAACCAGTCTTTATTACCTTTATAATGATTCGATCGTTTGCAAGCCCTTCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                      <  2:3973142/137‑1 (MQ=255)

                                                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                      >  1:2272686/1‑140 (MQ=255)

                                                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGATACTTCAACTTCGCAAGACAt                                                      >  1:5053321/1‑140 (MQ=255)

                                                                                    tttAATCATAACCTTAAATGTACGCGTACTGGTCTTTTTTAAATTAACAATTCTTTATTACCTTTATAATTTTTCTAGCGTATGAAAGCCCTACCCAAACACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                     <  2:679178/138‑1 (MQ=255)

                                                                                       aaGCATAACCTTAAAAGTACTCGGACAGGTATTATATAAATGAACCAGTCTTTATTACCTTTATCATTATTCGATCTTATCCAACCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaaca                                                  <  2:4137240/140‑1 (MQ=255)

                                                                                        aGCATAACCTTCATAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCCAAAACGATAAAACTACTCTGCTTCTTAAAGTTCGCAAGCCCTaacaa                                                 >  2:2640424/1‑140 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTCCCTGTATCATGATTCGATCGTAAGCAAGCCCTTCCCAAAAACTATAAAAAGAATCTGCTTCTTCAATTTCGCAAGACATAACAAAttt                                             >  1:576677/1‑139 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                             >  2:3027327/1‑140 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACACTGCTTCTTAAAGTTAGCAAGACATAAAAAATtg                                             >  1:210279/1‑140 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATAATGATTCGATCGTATGCAATCCCATCCCAAAAACGAAAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACttt                                             >  2:2155883/1‑139 (MQ=255)

                                                                                               tctacAAAGTACTCGGACCGGTGTTCTAAAAATCAACCAGTCTTTATTACCTGTATACTGATTCGATCGTCTCAACCCCCTGCCCAAACCCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:4236587/136‑1 (MQ=255)

                                                                                               ctttCAATTTACTCGGAATGGTTTTATATAAATTAACCCGTGGTTTTTCCCGTTATCTTTTTTCGTGCGTTTGCAACCCCGTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:1366277/138‑1 (MQ=255)

                                                                                               ccTTCAACTTCCTCGCACAGTTCTTCTAAAAAGGACCCTTTCTTTATTACGTTTATATTGATTCCATCGTTGGACAGCCGGTCCCAAAACCCTTACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:60679/140‑1 (MQ=255)

                                                                                               ccTTCAACGTACTCGGACATGTATTCTATAAATGACCCAGTCGTTTTTACCTTTATAATGATTCGATCTTATGCAAGCCCTTCCCAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:2912352/140‑1 (MQ=255)

                                                                                               ccTTCAAAGTACCGGGACCGGTCTTCTACAAAGTACCCCGTCGTTTTTACCTGTATCAGTTTTCGATGGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:4828202/140‑1 (MQ=255)

                                                                                               ccTTAAACGTCCTCGGTCCGGTCTTATATAAATGAACCAGTGGTTTTTACCTGTATAATGATACGATGGTATGCAAGCCCTACCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:3910895/140‑1 (MQ=255)

                                                                                               ccTACAAAGTACTCGTACAGTTCTTCTAAAAATCAACCATAGCTTATTACATGTATCATGATTCGATCGTATGCACCCCCTTCCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:194853/140‑1 (MQ=255)

                                                                                               cattCAAATTACTGGGACAGTTCTTATATAATTGAACCATTCTTTATTACCTGTAACATTTTTCGATCGTATGCACGCCATTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                          <  2:5060598/138‑1 (MQ=255)

                                                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                     >  1:1105616/1‑140 (MQ=255)

                                                                                                          cTCGGACTGGTTTTCTATAAATCAACCAGTCGTTATTCCGTGTATAATGATTCTATCGTAGGCAAGCCCTTCCAAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                               <  2:1436745/140‑1 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                               >  1:3520260/1‑140 (MQ=255)

                                                                                                           tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAATAGc                              >  1:3953448/1‑140 (MQ=255)

                                                                                                              caCAGTTATTCTATAAATCAACCATGCTTTATTACCTTTATCATGATTCTATCTTATGCACGCCCTCCCAAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                           <  2:3004714/139‑1 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAAGAGCGATCATg                       >  1:2509305/1‑140 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATAATg                       >  1:32010/1‑140 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGCCATAACAACTTGATGAGGCTTAAGAGCGATCATg                       >  1:378768/1‑140 (MQ=255)

                                                                                                                         tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAAGCCATAACAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                <  1:1020851/140‑1 (MQ=255)

                                                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg           >  2:4566636/1‑140 (MQ=255)

                                                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg           >  1:1609062/1‑140 (MQ=255)

                                                                                                                                 ccccAGTCGTTATTACCTTTTTATTCTTTCTATCTTATCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:4248208/138‑1 (MQ=255)

                                                                                                                                 cacacgTCTTTATTACGTTTATCTTTATTCCATTGTATCCAACCCTTTCCCAAAAAGCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:1269593/135‑1 (MQ=255)

                                                                                                                                 accctggcGTTATTCCCTTTATCATTATTCGCGCGTATCCACGCCCTCCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:4727446/133‑1 (MQ=255)

                                                                                                                                 aaCCCGTGGTTATTCCCTGTATCTGTATTCGATCGTCGGCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:4988455/140‑1 (MQ=255)

                                                                                                                                 aaCCCGTCTTTATTCCTTTTATCATTATTCTCGGGTTGGAAAGCCCTGCCAAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:1664125/140‑1 (MQ=255)

                                                                                                                                 aaCCAGTGTTTTTTACTTTTATAATTATTCTATCTTTTCCACCCCCTACCAAAAAAACAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa        <  1:4181102/140‑1 (MQ=255)

                                                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt      >  2:2089082/1‑140 (MQ=255)

                                                                                                                                     aGTCTTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTTGATGAGGCTTAAGATCGATAATGTCTCAATTGACGAAACAGc    >  2:4503150/1‑140 (MQ=255)

                                                                                                                                     aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc    >  2:3519517/1‑140 (MQ=255)

                                                                                                                                       tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACGATAAAACGTCTGTGCTTCTTCAAGTTAGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATGTCTAAATTTCCGATACTGCtt  >  1:4365490/1‑140 (MQ=255)

                                                                                                                                       tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt  >  1:4903794/1‑140 (MQ=255)

                                                                                                                                       tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACAGCtt  >  1:4026079/1‑140 (MQ=255)

                                                                                                                                       tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGGTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAATGCtt  >  1:4652075/1‑140 (MQ=255)

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTT  >  NZ_CP034943/3851083‑3851357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`