BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,234	C→A	5.1%	M47I (ATG→ATT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,234	0	C	A	5.1%	981.8 / 2.7	405	M47I (ATG→ATT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (260/105); new base A (8/32); total (268/137)
Fisher's exact test for biased strand distribution p-value = 3.48e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 407 total aligned reads displayed.

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851095‑3851371

cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                                           >  1:2507112/1‑140 (MQ=255)

   cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATg                                                                                                                                        >  2:2053057/1‑140 (MQ=255)

      ggTCCCAATCTGCTCTCTGTAAGAGGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  2:4127124/139‑1 (MQ=255)

        tCCCACTCTGCTCTCTGTAATATGGATTCGAAATGGAATTCTCTTTTAGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTGATTACCTGTATAATGaatag                                                                                                                                   >  1:4127810/1‑136 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:4041889/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  1:1551625/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:3689874/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:101998/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTAACTGTATCATGATTCg                                                                                                                                   >  2:4177069/1‑140 (MQ=255)

          ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                 <  2:2382117/140‑1 (MQ=255)

           cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  1:3685374/140‑1 (MQ=255)

           cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  1:1863186/140‑1 (MQ=255)

           cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                >  1:4661426/1‑140 (MQ=255)

           cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  1:2430461/140‑1 (MQ=255)

            aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCCAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:350152/140‑1 (MQ=255)

            aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:812293/140‑1 (MQ=255)

            aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:1829411/140‑1 (MQ=255)

             aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                              >  2:2819624/1‑140 (MQ=255)

             aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                              >  2:604108/1‑140 (MQ=255)

                 gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:4934104/140‑1 (MQ=255)

                 gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:4717854/140‑1 (MQ=255)

                 gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:1497189/140‑1 (MQ=255)

                 gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:2599048/140‑1 (MQ=255)

                  ctctctGTAATATGGATTTGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAACGTATGCa                                                                                                                         >  2:2514677/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                         >  1:48632/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:2032638/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:1211670/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:1450031/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:4214556/1‑140 (MQ=255)

                    ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAg                                                                                                                       >  1:274031/1‑140 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                    >  1:614415/1‑140 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                    >  1:4804700/1‑140 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTAGATCGTATTCAATccc                                                                                                                    >  1:2927271/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTccc                                                                                                               >  2:1438147/1‑140 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                            <  1:4289094/140‑1 (MQ=255)

                                 aTTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATTCAAGCCCTTCACaaaaa                                                                                                          >  1:1996392/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTAGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:1556155/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                                          >  1:498963/1‑108 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  1:4563651/113‑1 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  2:2738588/113‑1 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  1:2371612/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:1925102/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:4830921/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:343715/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTTAAAAGTACTCGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTAGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                          >  2:711194/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACg                                                                                                        >  1:3265150/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGATTTATCACCTTGTAAGCATAACATTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATAGTAAGCAACACCTTCCCAAAAACg                                                                                                        >  1:4176410/1‑140 (MQ=255)

                                              ttCACTTTTCGCTATTTTTATCCCCTTTTAAGAATACCCTACAAAGTACTCGGACTGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATTATTCTATCGTATGCACCCCCTCCCCAAAACCGATAACACGAct                                                                                             <  2:427321/140‑1 (MQ=255)

                                                ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACCAcact                                                                                           >  1:338520/1‑140 (MQ=255)

                                                   ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGct                                                                                        >  1:4457699/1‑140 (MQ=255)

                                                          tatttttATCACATTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCaa                                                                                 <  2:3866523/137‑1 (MQ=255)

                                                                       ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGATACTTCAACTTCGCAAGACAt                                                                    >  1:5053321/1‑140 (MQ=255)

                                                                        tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCACAAAAACGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACaaa                                                                   >  1:1645958/1‑138 (MQ=255)

                                                                           acgaaTAACCATCAAAGTAAGCGGACAGGTCTTCTATAAATCAACCTGTCGTTTTTACCTGTATCATTTTTCGATCGTATCCAAGCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaaca                                                                <  2:2182313/136‑1 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaacatt                                                           >  1:4736374/1‑137 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  2:33616/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                                           >  2:3027327/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCAGCTTCTTCAAGTTCGCAACACATaacaacatt                                                           >  1:3194824/1‑137 (MQ=255)

                                                                                   ccaaaaaaGTACTCCGACAGTTCTTCAATAAATGAACCAGTCTTTATTACCTGTAACTTTATTCTATCGTCTGAAAGCCCTACCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:2097137/135‑1 (MQ=255)

                                                                                   ccTTCAATGTACTCGCACAGGTGTTATATAAATCAACCAGTCTTTATTCCCTGTATCATCATTCGATCGTTTGCAACCCCTACCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:1416993/140‑1 (MQ=255)

                                                                                   ccTTCAACGTACTCGGACATGTATTCTATAAATGACCCAGTCGTTTTTACCTTTATAATGATTCGATCTTATGCAAGCCCTTCCCAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:2912352/140‑1 (MQ=255)

                                                                                   ccTTCAACGTACTCGGACAGGTCTTATATAAATCAACCAGTCGTTATTACCTTTATCATTATTCGATCGTTTCCAAGCCCTCCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACACAACAACTtgatg                                                        <  2:3953448/140‑1 (MQ=255)

                                                                                   ccTTCAAAGTACTCGTACTGTTCTTAAATAAATTAACCAGTCTTTATTACCTGTATCATTTTTCGATCGTATGCAAGCCCTTCCCAAAAACAATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:3550908/140‑1 (MQ=255)

                                                                                   ccTACAAATTACTCGGACAGGTATTCTATAAATGAACCAGTCTTTTTTACCTTTATAATGTTTCGATCTTATGCAAGCCCTTCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:5039884/140‑1 (MQ=255)

                                                                                      tCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAgg                                                     >  1:4927787/1‑140 (MQ=255)

                                                                                        aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                   >  1:1105616/1‑140 (MQ=255)

                                                                                             aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAaga                                              >  1:881844/1‑140 (MQ=255)

                                                                                             aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAaga                                              >  1:549750/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAagag                                             >  1:2605710/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                             >  2:4586260/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAagag                                             >  2:1298102/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTGGATCGTAGTCAAGCCCGTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAAGTTATGAGGCTTAagag                                             >  1:721785/1‑140 (MQ=255)

                                                                                               tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAATAGc                                            >  1:3953448/1‑140 (MQ=255)

                                                                                                 ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGa                                          >  1:3574131/1‑140 (MQ=255)

                                                                                                  gACAGGTTTAATATAAATCAACCCGTCGTTATTACCTGTATAATTATTCGTTGGTTTGCAAGCCCTACCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:750489/140‑1 (MQ=255)

                                                                                                  caCAGTTATTCTATAAATCAACCATGCTTTATTACCTTTATCATGATTCTATCTTATGCACGCCCTCCCAAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:3004714/139‑1 (MQ=255)

                                                                                                  caCAGGTATTAAATAAATGAACCTGTCGTTATTACGTGTTTCATTTTTCGATCTCATGCAAGCCCTCCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:5161962/139‑1 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGctgtctct                                     >  1:2573419/1‑133 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATAAtt                                     >  1:3120993/1‑139 (MQ=255)

                                                                                                        tcttctATAAATGAACCAGTCGTTAGTACCTGTATCATGATTAGATCGTATGCCACCCCTTCCCAAAAACGAAAAAACGACTCTGCATCTTCAATTTCGCAATACAAAACAACATGATGAGGCTTAAGAGCGATCAAGTc                                   >  1:1763273/1‑140 (MQ=255)

                                                                                                           tataaaaaTTAACCCGACGTTATTACCTGAAACATCATTCCATCGTCTGAAACCCCTACAAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                <  1:3745685/135‑1 (MQ=255)

                                                                                                            ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGATGCTTAAGATCGATCATGTCTaaa                               >  1:3861154/1‑140 (MQ=255)

                                                                                                             tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAAGCCATAACAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                              <  1:1020851/140‑1 (MQ=255)

                                                                                                             tataAATTACCCTGTCTTTATTCCCTTTATATTTTTTCGATCGTATCAACGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                              <  1:4394771/140‑1 (MQ=255)

                                                                                                              ataAATGAACCAGTCTTTATTACCTTTATCATTATGGGATCGTATGCAAGCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAtt                             <  2:3587957/140‑1 (MQ=255)

                                                                                                                     cacacgTCTTTATTACGTTTATCTTTATTCCATTGTATCCAACCCTTTCCCAAAAAGCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  1:1269593/135‑1 (MQ=255)

                                                                                                                     aacacgTCTTTTTTACGTGTTTAATTATTCGATGGTCTGCAACCCCCACCCAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  1:2083842/135‑1 (MQ=255)

                                                                                                                     aaCCTGTCGTTTTTACCTGTATCATGTTTCGATCTTAGGAACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:195453/140‑1 (MQ=255)

                                                                                                                     aaCCAGTCGTTATTACCTGTATCATTATTCGAGCGTATGCACCCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:4736374/140‑1 (MQ=255)

                                                                                                                       cctgTCTTTTTTACTTGTATCATGTTTCCATCTTAGGAACGCCCTCCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                    <  1:5138438/137‑1 (MQ=255)

                                                                                                                         aGTCTTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTTGATGAGGCTTAAGATCGATAATGTCTCAATTGACGAAACAGc                  >  2:4503150/1‑140 (MQ=255)

                                                                                                                           tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACGATAAAACGAATATGCTTATTCAATTTCGCAAGACAAAACAACTTGATGTTGCTTAAGAGCGATCATGTCTAAATTTCCGATaaagcgt                >  1:3472910/1‑134 (MQ=255)

                                                                                                                           tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACAGCtt                >  1:4026079/1‑140 (MQ=255)

                                                                                                                              ttattcCCTGTATCATGATTCGATCGTATGCACGCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata             <  2:3778837/140‑1 (MQ=255)

                                                                                                                                attaCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGc           >  2:321725/1‑140 (MQ=255)

                                                                                                                                  taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg         >  2:65591/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAGGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  1:2222140/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  1:2845897/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTCGATCGTATGCAAGCCATTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAGCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  2:2463458/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTAGATCGTATGCAAGCCCTTCCAAAAAACGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGATTAAGAGCGATCATGTCTAAATTGACGATACTGCTTATAGCGGTTAttta  >  2:4445889/1‑139 (MQ=255)

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851095‑3851371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`