Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,085 C→A 13.1% Q33K (CAA→AAA)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,0850CA13.1% 636.2 / 27.7 279Q33K (CAA→AAA) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (129/104);  new base A (2/43);  total (131/148)
Fisher's exact test for biased strand distribution p-value = 1.43e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCT  >  NZ_CP034943/548947‑549222
                                                                                                                                          |                                                                                                                                         
gTTTTCAAAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCa                                                                                                                                          <  1:3814310/140‑1 (MQ=255)
                  aTACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTccc                                                                                                                        <  2:1303063/140‑1 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTTAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:2349855/140‑1 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:631756/140‑1 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:475557/140‑1 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:3163760/140‑1 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  2:4977956/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  2:2327083/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  1:1759448/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  1:3610628/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCAAAAAGaatac                                                                                                          >  2:2350502/1‑136 (MQ=255)
                                 acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                                                         <  2:3981155/140‑1 (MQ=255)
                                  caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAGTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                        >  2:2050075/1‑140 (MQ=255)
                                     cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCATCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                     >  2:5097710/1‑140 (MQ=255)
                                     cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCGGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                     >  2:895711/1‑140 (MQ=255)
                                        aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  2:4562149/140‑1 (MQ=255)
                                        aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  2:3467520/140‑1 (MQ=255)
                                        aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  1:3437542/140‑1 (MQ=255)
                                        aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  2:4454929/140‑1 (MQ=255)
                                          aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATTCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAATACTCCTGGCGAGTCa                                                                                                >  1:2542235/1‑140 (MQ=255)
                                          aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                >  1:2277457/1‑140 (MQ=255)
                                          aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                >  1:2139688/1‑140 (MQ=255)
                                          aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAGTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                >  1:3906489/1‑140 (MQ=255)
                                           tcgagatCACGCAGTGTTTGACTAGAAATCTGGATATTCCGCATTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAg                                                                                               <  2:2313465/135‑1 (MQ=255)
                                           tGGATATCACGCCTTTTTTGAATAGACATCTGGATATTCCGCTTTCTCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAg                                                                                               <  2:2478931/140‑1 (MQ=255)
                                           tGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCTCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAg                                                                                               <  2:4243451/140‑1 (MQ=255)
                                           tGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAg                                                                                               <  2:1375528/140‑1 (MQ=255)
                                           tGGATATCACGCATTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAg                                                                                               <  2:241819/140‑1 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  2:3515568/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  2:4863858/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  1:4262346/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTAGCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  1:4770877/1‑140 (MQ=255)
                                                atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                          >  2:3108763/1‑140 (MQ=255)
                                                    cGCCTTTTTTGAATAGAAATCTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                      <  1:1332286/140‑1 (MQ=255)
                                                    cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                      <  1:887850/140‑1 (MQ=255)
                                                    cGCCTTTGTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCGATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTTCCAAACGACTCCTGGCGAGTCAGCTTTCCATc                                                                                      <  2:3831662/140‑1 (MQ=255)
                                                       cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATAAGt                                                                                   >  2:1431735/1‑140 (MQ=255)
                                                           ttttAATATAAATATGGATTTTCCGCTTTATCAGCTGATTTATCGATATTTCAAAGAAAAAATGCATCAGGGCCTTATTAAAAAAGTATTTAAGCTCCCTTCCAAAAGCCTCCTGGCGAGAAAGCTTTCCATCAGTCaaa                                                                               <  2:2535032/140‑1 (MQ=255)
                                                           ttttAATAGAAAAGTGGATTTTCCGCTTTAACTCCAGCTTTTTCGCTATTTCAAACAAAACATGCATCAGGGGCTTATTAAAAATGGAATGAAGCTCCCTTCCAAACGAACCCTGGCGAGTCAGCTTTCCTTCAGTCaca                                                                               <  2:2336988/140‑3 (MQ=255)
                                                           tttGAATATAAATCTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCCGGGGCGTATTCAAAAAGGAATGACGCGCCCTTCAAAACGACTCCTGGCGAGTCAGCTTTCCATCAGCCaaa                                                                               <  2:3960701/140‑1 (MQ=255)
                                                            ttGAATAGAAATCTGTATATTCCGCTTTAACAGAAGCTTTATCGCTATTTAAAAGAAAAAATGCATCCGGGGGTTATTCAAAAAGGAATTAAGCTCCATTCAAAACGACGCCTGGCGAGTCAGCTTTCCATCAGacacac                                                                              <  2:2094180/140‑1 (MQ=255)
                                                              caaaataaaTCTGGATATTCCGCTTTATCCCCAGCTTTATCGATATTACAAACAAAAAATCCATCAGGGGCTTATAAAAAAAGCAATCAAGCTCCCTCCCAAAAGACTCCGGCCGAGTCAGCTGCCCAGCCGTCACACGa                                                                            <  2:1559078/134‑1 (MQ=255)
                                                               aaTAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGAc                                                                           >  2:838890/1‑140 (MQ=255)
                                                                 tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                         >  2:4273752/1‑140 (MQ=255)
                                                                        gtGGATATTCCGCTTTAACACCAGCTTTAGCGATATTTCAAATAAAACGTGCATCCGGGGCTTATTCAAAAAGCAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACg                                                                  <  2:599468/139‑1 (MQ=255)
                                                                         tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAATAAAACATGCATCACGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCCAACGACTGTAGACCGt                                                                 >  1:4049528/1‑140 (MQ=255)
                                                                             tatTCCGTTTTAGAATCAGCTTTAGCGATTTTTCAAACAAAACATGCATCCGGGGCTTATTCAAAAAGGAATTAAGCTCCATTCCAAAAGACTCCTGGCGAGTAAGCTTTCCATCCGTCAAACGACTGTAGAACGTGCCt                                                             <  2:3645089/140‑1 (MQ=255)
                                                                              atTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTa                                                            >  2:2855883/1‑140 (MQ=255)
                                                                                tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACTTGCCTATg                                                          >  1:4228648/1‑140 (MQ=255)
                                                                                tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                          >  1:2783797/1‑140 (MQ=255)
                                                                                  cggtttATCAGCAGCTTTATGGCTATTTCAAACAAACAATGAATCCGGGGGTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAATACTCCGGGCGTGTCAGCTTTCCATCAGACAAACGACTGTAGAACGTGCCTATGaa                                                        <  2:2499779/137‑1 (MQ=255)
                                                                                    cTTTATCAGCAGCTTTATCGATATTTAAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGATTGAAGCCCCCTTCCAAAACACTCGGGGGGAGTCAGTTTTCCATCCGTCAAACGACTGTAGAACGTGCCTATGAACa                                                      <  2:4500755/140‑1 (MQ=255)
                                                                                     tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                     >  2:1968701/1‑140 (MQ=255)
                                                                                     tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                     >  2:4943936/1‑140 (MQ=255)
                                                                                       tATCCGCAGCTTTAGCGCTATTCAAAACAAAACATGCATCCGGGGCGTATTAAAAACGGAATGAAGCGCCCTGCCACAAGACGCCTGGCGAGTCAGCTTACCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                                   <  2:2999471/140‑1 (MQ=255)
                                                                                         tCAGCAGCTTTATCGATATTTCAAAGAAAACATGCATCCGGGGCGTATTCAAAAAGTAATGAAGCTCCGTTCCAAACGACTCCTGGCGAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                 <  2:3023823/140‑1 (MQ=255)
                                                                                         acagcagCTTTATCGCTATTACACACAAAACATGCATCAGGGGCGTATTCAAACAGCAATCAAGCTCCCTTCCAAAACACTCCTGGCGAGACAGCTTGCCAGCAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                 <  2:3316976/139‑1 (MQ=255)
                                                                                              tgcTTTATGGATTTTTAAAACAAACCATGACTCCGGGGCTTTTTAAAAAAGGAATGAAGCTCCCTTCAAAAACACGCCGGGCGAGTAAGCTTTCCAACCGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  1:4273752/139‑1 (MQ=255)
                                                                                              agttttATCGCTATTTCAAATAAAACATGCATCAGGGGCTTATTCAAAATGTAATGAAGCTCCTTTCCAAAAGCCTCCTGGCGAGACAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  2:4169208/137‑1 (MQ=255)
                                                                                              agcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            >  2:4532169/1‑140 (MQ=255)
                                                                                              agcTTTATCGATTTTTAAAACAAAACAGGCATCCGGGGCGTATAAAAAAAGGAATTAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTCCCACCAGAAACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  1:4449123/140‑1 (MQ=255)
                                                                                              agcTTTATCGATATTACAAACAAACCATGAATCCGGGGCTTATAAAAAAAGTAATCAAGCGCCCTCCAAAACCACTCCTGGCGAGTCAGCTTTCCAACAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  1:402084/140‑1 (MQ=255)
                                                                                              agatttATCGCTATTTAAAACAAAACATGCATCCGGGGGGTATTAAAAAAGTACTGACGCTCGCTTCCAAACGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  2:548879/137‑1 (MQ=255)
                                                                                                cTTTCTCGCTATTTCAAACAAAACATGCATCCGGGGCGTATACAAAAAGCAATGACGCGCCCTTCAAAACCCCCCCTGGCGCGTCAGCTTCCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:766380/140‑1 (MQ=255)
                                                                                                cTTTATCGCTATTTCAAACAAAACATGCAACAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTGCCAAACGACTCCTGGCGAGACAGCTGTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:1027855/140‑1 (MQ=255)
                                                                                                cTTTATCGATATTCAAAATAAAACTTGAATCAGGGGCTTATAAAAAATGTAATGACGCTCCCTTCCAAAAGACTCCTGGCTCGTAAGCTTTCCATCTGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:1147016/140‑1 (MQ=255)
                                                                                                     tCGCTATTTCACAGAAAACAGCCAGCCGGGGCGTATTCAAAACCTAATCAAGCTCCCTTCCAAAAGACGCCTGGCGAGTCAGCTTTCCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  2:4115540/140‑1 (MQ=255)
                                                                                                     tCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAACGGAATGAAGCTCCCTACCAAAAGACTCCGGGCGAGTCAGCTTTCCACCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  1:5018722/140‑1 (MQ=255)
                                                                                                     tCGCTATTTCAAACAAAACATGCATCCGGGGCGTATACAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  2:710064/140‑1 (MQ=255)
                                                                                                     tCGATATTACAAACAAAACATGAAGCCGGGGCTTATAAAAAATGTAATTAAGCGCCCTTCCAAACCACCCCTGGGGATTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  1:518546/140‑1 (MQ=255)
                                                                                                     acGCTATTAAAAACAAAACATGCACCCGGGGCTTATCCAAAAAGGAAGGAAGCGCCCTCCCAACACACCCCGGGCTAGTCAGGTTTCCAACAGAAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  2:1841199/139‑1 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  2:2378128/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  1:1438090/1‑140 (MQ=255)
                                                                                                           tttCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCg                               >  2:3705409/1‑140 (MQ=255)
                                                                                                                        ctcaaTCCGGGGCGTATTAAAAAAGCAATGAAGAGCCCTTCAAACAGCCCCCTGGCGAGGCAGGTTTCCATCCGTCAAACGACTGGAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaag                  <  1:2099039/136‑1 (MQ=255)
                                                                                                                        aTGAATCCGGGGCTTATAAAAAAAGTAATGAAGCTCCCTTCAAAACGACTCCTGGCGAGTCAGCTTTCCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaag                  <  1:233490/140‑1 (MQ=255)
                                                                                                                            aTCTGGGGCTTATTAAAAAAGTAATGACGCGCCCTTCCAAATGACTCGTGGGGAGTCAGCTTTCCATCTGGCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGc              <  2:4292922/140‑1 (MQ=255)
                                                                                                                            aTCAGGGGCTAATTCAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGATTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGc              <  2:3223769/140‑1 (MQ=255)
                                                                                                                             tCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCg             >  1:4568886/1‑140 (MQ=255)
                                                                                                                               atcggCGTATTCCAAAAGTAATTAAACTACCTACCAAAAGACTCCTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATCTCGTGAGCAAGCCAAGAAGCGGa           >  2:4681995/4‑140 (MQ=255)
                                                                                                                                  ggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATgg        >  2:665973/1‑140 (MQ=255)
                                                                                                                                   gctttttCAAAACGGAATGAAGCGCCGTTCCAAACGACTCCGGGCGAGTCAGCTTACCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGt       <  2:3866110/135‑1 (MQ=255)
                                                                                                                                   gagTATTAAAAAAGGAATGAAGCTCCCTACCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGt       <  2:3860155/138‑1 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAATCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAAGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATAGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:2676405/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTc                                                                                                 >  1:2836179/1‑47 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:1058864/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:1495745/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:2224890/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:1511516/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:748674/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:554913/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:3747208/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:2632184/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:3387300/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:3310748/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  1:2896561/1‑140 (MQ=255)
                                                                                                                                       aTTCAAAAAGGTTTTAAGCTCCCTTCCAAAAGAGGCCTGGCGAGTGAGGTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGc   <  2:1707080/140‑1 (MQ=255)
                                                                                                                                       aTTAAAAACGGATTGAAGCTCCATTCAAAAAGACTCGTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGc   <  2:2821337/140‑1 (MQ=255)
                                                                                                                                        ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt  >  1:1253447/1‑140 (MQ=255)
                                                                                                                                        ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt  >  1:1137744/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCT  >  NZ_CP034943/548947‑549222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: