BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	8.1%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	8.1%	934.4 / 8.0	400	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (253/86); new base T (10/44); total (266/134)
Fisher's exact test for biased strand distribution p-value = 3.35e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851098‑3851371

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  2:36686/140‑1 (MQ=255)

   ggTCCCAATCTGCTCTCTGTAAGAGGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  2:4127124/139‑1 (MQ=255)

     tCCCACTCTGCTCTCTGTAATATGGATTCGAAATGGAATTCTCTTTTAGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTGATTACCTGTATAATGaatag                                                                                                                                   >  1:4127810/1‑136 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAACTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:3538848/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:4041889/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  1:1551625/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                   >  2:101998/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTAACTGTATCATGATTCg                                                                                                                                   >  2:4177069/1‑140 (MQ=255)

       ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                 <  2:2382066/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  1:1863186/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  1:2430461/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  2:2512009/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                >  1:4661426/1‑140 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                <  2:3023432/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:1829411/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:812293/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:4488909/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATACTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                               <  2:3850798/140‑1 (MQ=255)

          agcTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCGTTTCGCTAGTTTTATCAACTTGTAAGGATAACCTTCAAATAACTCTGACAGGTCTTCTATAAATGAACCAGACGGTATTAACTGGATCATAATTAGATAGt                                                                                                                              >  2:3483916/3‑140 (MQ=255)

          aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                              >  2:604108/1‑140 (MQ=255)

              gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:1893745/140‑1 (MQ=255)

              gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:3173056/140‑1 (MQ=255)

              gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                          <  2:1497189/140‑1 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                         >  1:48632/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:4214556/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:2032638/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:1450031/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  2:4727446/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                         >  1:1211670/1‑140 (MQ=255)

                 ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAg                                                                                                                       >  1:274031/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                    >  1:614415/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                    >  1:4804700/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTAGATCGTATTCAATccc                                                                                                                    >  1:2927271/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTccc                                                                                                               >  2:1438147/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTccc                                                                                                               >  2:4704327/1‑140 (MQ=255)

                            ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                            <  1:4289094/140‑1 (MQ=255)

                              aTTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATTCAAGCCCTTCACaaaaa                                                                                                          >  1:1996392/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTAGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:1556155/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                     <  1:4563651/113‑1 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:2052245/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  1:3778837/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  1:3704535/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  1:2371612/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                          >  2:3431754/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTTAAAAGTACTCGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTAGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                          >  2:711194/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACg                                                                                                        >  1:3265150/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGATTTATCACCTTGTAAGCATAACATTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATAGTAAGCAACACCTTCCCAAAAACg                                                                                                        >  1:4176410/1‑140 (MQ=255)

                                           ttCACTTTTCGCTATTTTTATCCCCTTTTAAGAATACCCTACAAAGTACTCGGACTGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATTATTCTATCGTATGCACCCCCTCCCCAAAACCGATAACACGAct                                                                                             <  2:427321/140‑1 (MQ=255)

                                             ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGAatct                                                                                           >  2:2799637/1‑140 (MQ=255)

                                             ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACCAcact                                                                                           >  1:338520/1‑140 (MQ=255)

                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGct                                                                                        >  1:4457699/1‑140 (MQ=255)

                                                  ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCATCCCAAAAACGATAAAACGACTCTGcttc                                                                                      >  1:3587957/1‑140 (MQ=255)

                                                  ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGcttc                                                                                      >  2:2909511/1‑140 (MQ=255)

                                                                   cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACa                                                                     >  1:249348/1‑140 (MQ=255)

                                                                   cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                     >  2:4871638/1‑140 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACaa                                                                    >  2:3351529/1‑139 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGATACTTCAACTTCGCAAGACAt                                                                    >  1:5053321/1‑140 (MQ=255)

                                                                     tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCACAAAAACGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACaaa                                                                   >  1:1645958/1‑138 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaacatt                                                           >  1:4736374/1‑137 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  2:3719447/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCAGCTTCTTCAAGTTCGCAACACATaacaacatt                                                           >  1:3194824/1‑137 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATAATGATTCGATCGTATGCAATCCCATCCCAAAAACGAAAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACttt                                                           >  2:2155883/1‑139 (MQ=255)

                                                                                ccTTCAATGTACTCGCACAGGTGTTATATAAATCAACCAGTCTTTATTCCCTGTATCATCATTCGATCGTTTGCAACCCCTACCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:1416993/140‑1 (MQ=255)

                                                                                ccTTCAATGTACGCGGACCGGTGTTCTATAAATGACCCTGTGGTTATTACCTGTATCATTTTTGGAGCGTATGCAACCCCTCCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:3560685/140‑1 (MQ=255)

                                                                                ccTTCAAAGTACCGGGACCGGTCTTCTACAAAGTACCCCGTCGTTTTTACCTGTATCAGTTTTCGATGGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:4828202/140‑1 (MQ=255)

                                                                                ccTCCAAAGTCCTCGCACAGTCGTTATATAAATCAACCAGTGTTTATTACCTGTATCATGTTTCGTTCGTATGCAAGCCCTCCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:2970411/140‑1 (MQ=255)

                                                                                ccTACAAATTACTCGGACAGGTATTCTATAAATGAACCAGTCTTTTTTACCTTTATAATGTTTCGATCTTATGCAAGCCCTTCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:5039884/140‑1 (MQ=255)

                                                                                ccTACAAAGTACTCGTACAGTTCTTCTAAAAATCAACCATAGCTTATTACATGTATCATGATTCGATCGTATGCACCCCCTTCCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:194853/140‑1 (MQ=255)

                                                                                     aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                   >  1:1105616/1‑140 (MQ=255)

                                                                                          aCTCGGACTGGTTTTATATAAATCAACCAGTCTTTATTACCTTTATCATTATTCTATCTTATGCAAGCCCTTCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAaga                                              <  2:3921415/140‑1 (MQ=255)

                                                                                          aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAaga                                              >  1:881844/1‑140 (MQ=255)

                                                                                          aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAaga                                              >  1:549750/1‑140 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAagag                                             >  1:2605710/1‑140 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAagag                                             >  2:1298102/1‑140 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                             >  1:3520260/1‑140 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTGGATCGTAGTCAAGCCCGTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAAGTTATGAGGCTTAagag                                             >  1:721785/1‑140 (MQ=255)

                                                                                            tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAATAGc                                            >  1:3953448/1‑140 (MQ=255)

                                                                                               gACAGGTTTAATATAAATCAACCCGTCGTTATTACCTGTATAATTATTCGTTGGTTTGCAAGCCCTACCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:750489/140‑1 (MQ=255)

                                                                                               caCAGGTATTAAATAAATGAACCTGTCGTTATTACGTGTTTCATTTTTCGATCTCATGCAAGCCCTCCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:5161962/139‑1 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGctgtctct                                     >  1:2573419/1‑133 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATAAtt                                     >  1:3120993/1‑139 (MQ=255)

                                                                                                    gTCTTATAAAAATGAACCCGTCGTTTTTACCTGTATAATTATTCGAGCGTATGCAACCCCGCCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGt                                    <  2:4417934/140‑1 (MQ=255)

                                                                                                     tcttctATAAATGAACCAGTCGTTAGTACCTGTATCATGATTAGATCGTATGCCACCCCTTCCCAAAAACGAAAAAACGACTCTGCATCTTCAATTTCGCAATACAAAACAACATGATGAGGCTTAAGAGCGATCAAGTc                                   >  1:1763273/1‑140 (MQ=255)

                                                                                                         ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGATGCTTAAGATCGATCATGTCTaaa                               >  1:3861154/1‑140 (MQ=255)

                                                                                                          tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAAGCCATAACAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                              <  1:1020851/140‑1 (MQ=255)

                                                                                                          tataAATTACCCTGTCTTTATTCCCTTTATATTTTTTCGATCGTATCAACGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                              <  1:4394771/140‑1 (MQ=255)

                                                                                                              aaTGAAACAGTCCTTAATACATGTATCATGATTCGATCGTATGCAAGCCCTTGACAAAAACGTTAAAACTCATCTGCTTATTCAATTTCGCAATAAAAAACAACTATATGAGGCTTAACAGCGAGCAAGGGTAAAtagac                          >  2:272395/1‑136 (MQ=255)

                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                         >  2:4566636/1‑140 (MQ=255)

                                                                                                                  cacacgTCTTTATTACGTTTATCTTTATTCCATTGTATCCAACCCTTTCCCAAAAAGCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  1:1269593/135‑1 (MQ=255)

                                                                                                                  acccATTCGTTATCACCTTTATCATTATTCGATCGTAGCCAACCACTCCCAAAAACCGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:2578107/138‑1 (MQ=255)

                                                                                                                  aacacgTCTTTTTTACGTGTTTAATTATTCGATGGTCTGCAACCCCCACCCAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  1:2083842/135‑1 (MQ=255)

                                                                                                                  aaCCCGTGGTTATTCCCTGTATCTGTATTCGATCGTCGGCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  1:4988455/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTATTACTTTTATCATGATTATATCTTATCCAAGCCCTACCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:2458242/140‑1 (MQ=255)

                                                                                                                    cctgTCTTTTTTACTTGTATCATGTTTCCATCTTAGGAACGCCCTCCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                    <  1:5138438/137‑1 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                    >  2:2089082/1‑140 (MQ=255)

                                                                                                                        tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACAGCtt                >  1:4026079/1‑140 (MQ=255)

                                                                                                                        tCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAATAGCGATCATGTCTAAATTTCCGATActtctt                >  1:4904330/1‑140 (MQ=255)

                                                                                                                               taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg         >  2:65591/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAGGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  1:2222140/1‑140 (MQ=255)

                                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  1:2845897/1‑140 (MQ=255)

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851098‑3851371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`