BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,945,908	T→G	9.5%	L388R (CTT→CGT)	EO946_RS20305 →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,945,908	0	T	G	9.5%	502.5 / 6.0	269	L388R (CTT→CGT)	EO946_RS20305	Na+/H+ antiporter
Reads supporting (aligned to +/- strand): ref base T (125/100); new base G (37/2); total (167/102)
Fisher's exact test for biased strand distribution p-value = 5.31e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

ccGGTTACAGGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCt                                                                                                                                             <  2:4157072/140‑1 (MQ=255)

   gTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttgt                                                                                                                                          <  2:4353598/140‑1 (MQ=255)

       cATTGAAGAATAACATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCGCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACATTgttgtt                                                                                                                                      <  1:1345464/140‑1 (MQ=255)

               cATACCATCAATTGGGGCGGGCTTAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  2:1201064/140‑1 (MQ=255)

               cATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  2:2696633/140‑1 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTCAGGGGGTCa                                                                                                                            >  1:3303884/1‑140 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTGTTGAGGGGCGCa                                                                                                                            >  1:2933553/1‑140 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTTTTAAGGGGCTCa                                                                                                                            >  2:1317146/1‑140 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCa                                                                                                                            >  1:4564313/1‑140 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTGAGGGGCTCa                                                                                                                            >  1:2123137/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCCGGGGCTCAc                                                                                                                           >  2:2229078/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCAc                                                                                                                           >  2:5196219/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTGTTCAGGGGCTAAc                                                                                                                           >  1:4455544/1‑140 (MQ=255)

                      tCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATc                                                                                                                       >  2:4055445/1‑140 (MQ=255)

                         aTTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGATACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAg                                                                                                                    >  2:910501/1‑140 (MQ=255)

                         aTTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCt                                                                                                                                             <  1:4093791/115‑1 (MQ=255)

                           tGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTTTTGTTCACGGGCTCACCATCAAAcc                                                                                                                  >  2:1569703/1‑140 (MQ=255)

                             gggcgggcTGAAGGGTTCTCTGTCCAGTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGc                                                                                                                <  2:101843/140‑1 (MQ=255)

                              ggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGct                                                                                                               >  1:3486149/1‑140 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTTTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                           >  1:4464455/1‑140 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                           <  2:2127923/140‑1 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCCcttctt                                                                                                           >  1:2239130/1‑140 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:4939581/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:480042/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:3524295/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:3630519/140‑1 (MQ=255)

                                          ggTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGtt                                                                                                   <  1:916593/140‑1 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCCTCAAGCCGCTTCTTGAACGGGTAgg                                                                                                >  1:5038542/1‑140 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAgg                                                                                                >  2:4732615/1‑140 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTGTTGTACgggttgg                                                                                                >  1:63988/1‑135 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCAGGGACGTGAAGACATTCTGGTTTTCGCATTCAGTGTCGTTTTTTTTTACCTTGTTGTTAAGGGGCTCACAATCAAACCGCTTCTTAAACTGATAgtt                                                                                               >  2:5062050/1‑138 (MQ=255)

                                                 tGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTGAGGGGCTCACCATAAAGCCGCTTCGTGAACGGTTAGGtgtg                                                                                            >  1:1720840/1‑140 (MQ=255)

                                                 tGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTTTTTTTCAGGGGCTCACCAACAAGCCCCTTCTTGAACCGTTAgggttt                                                                                            >  1:2703280/1‑136 (MQ=255)

                                                   tCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         >  2:2788275/1‑125 (MQ=255)

                                                      aTTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCGTGTTGTTCAGGGGGTCACCATAAATCCGCTTGTTGTACGGTTAGGTGTGTATCa                                                                                       >  1:2351387/1‑140 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTTGGTGTGAATCaaa                                                                                     >  1:3696120/1‑140 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaa                                                                                     >  2:3863535/1‑140 (MQ=255)

                                                          cACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTGTTCAGGGGCTCACCATAAAGCCGCTTCTTGAACAGTTAGGTGTGAATCaaaaa                                                                                   >  2:2284585/1‑140 (MQ=255)

                                                          cACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaaaa                                                                                   >  2:4397099/1‑140 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCCGGGGCTCAACATCAATCCGCTTCTTGACCGGTTAGGTGTGAAGCaaaaatta                                                                                >  1:4720866/1‑137 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaa                                                                                >  2:828803/1‑140 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTGACCATACAGCCGGGGAGTTTACGGGTAGGTGTGTATCaaaaatac                                                                                >  1:3879237/1‑137 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGTGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTTTTAAGGGGGTAACAAAAAAGCCCCATAATTTACTGTTAGGTGTGAATAAAAaataaga                                                                              >  1:1814051/1‑140 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTGAGGGGCTCACCATCAAGCCGGGTCTTGACCGGGTAGGTGTGAATCAAAaagacta                                                                              >  1:4031782/1‑137 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTCAGGGGCTCCCCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaaga                                                                              >  2:1448586/1‑140 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:3835469/92‑1 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGGTCAGGGGCTCACCCTCAAGCCGCGTCTTGAACGGTTGGGGGTGAATCAAAaataaga                                                                              >  1:2551362/1‑140 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATAAAGCCGCTTCTTGAACGGTTAGGTGTGAAACAAAaagaaga                                                                              >  2:1887205/1‑140 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTTTTGAGGGGCTCACCAACAAGCCGCGTCTTGAACGGTTAGGTGTGAAAAAAAaaaaaga                                                                              >  1:490754/1‑140 (MQ=255)

                                                                tGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCGTGTTGTTCAGGGGCTCACCAACAACCCGCGTGTTGAACGGGTAGGGGTGAATCAAAaaaaagaa                                                                             >  1:166032/1‑140 (MQ=255)

                                                                 gCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAATCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaagaag                                                                            >  2:2451480/1‑140 (MQ=255)

                                                                  cTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTTTTGCGGGGCTCACCATCCAGCCCGGTGTTGAACGGTTTGGTGTGTATAAAAAACAAGAAgg                                                                           >  1:3113610/1‑140 (MQ=255)

                                                                     aGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTGTTCAGGGGCTCACCAACAATCCGCTTCTTGAACGGGTAGGTGTGAAAAAAAAAGAATAAGGCaa                                                                        >  1:4744942/1‑140 (MQ=255)

                                                                     aGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGGTCCGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAAAAAAAAAGAAGAATGCaa                                                                        >  1:5125153/1‑140 (MQ=255)

                                                                     aGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTGTTGAGGGGCGCACCCACAAGCCGCGTGGTGGACCGGTAGGGGTGTATCAAAAAAAAGAAGGCaa                                                                        >  1:4620193/1‑140 (MQ=255)

                                                                     aGCCTGCCTAGAGACGTCCCGGGACGTGAAGACATTCTGGTTTTAGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCaa                                                                        <  2:2891286/140‑1 (MQ=255)

                                                                        ctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCa                                                                     <  1:515323/140‑1 (MQ=255)

                                                                         tgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  2:1942252/140‑1 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTTCCTTGTTTTTGAGGGGCTGACCAAAAATCCCCGTCATTAACGGTTTGGTGTTAATAAAAAACAATAATGCAAACacg                                                                   >  1:571730/1‑138 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                  >  2:2730896/1‑140 (MQ=255)

                                                                             tAGAGACTTCCCGGGCCGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTGGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGt                                                                <  2:2042420/140‑1 (MQ=255)

                                                                             tAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGt                                                                <  1:5183800/140‑1 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTGTTGGACGGTTTGGGGTGAATCAAAAAGAACACGGCAACCCGGAGTGTGAggt                                                         >  2:4438641/1‑139 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGCACGGTTAGGTGTGAATCAAAAAGAACAAGGCAACCAGGAGTATGAGGa                                                         >  2:741737/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTGTTGAGGGGGTCACCATCAAGCCGCTTGGTTTACGGTTTGGGGGGGATCAAAAATAAGAAGGCAACCCGGGGTATGTGGaa                                                        >  1:4252853/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCATTTTGTTAAGGGGGTCACCATAAAGCCGCGTCTTGTACGGTTAGGGGTTAATCAAAAAGAATAAGTCAAACAGGATGATGAGGaa                                                        >  1:1184923/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTc                                                                                                                                     <  1:781521/63‑1 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTGTTGAACGGTTTGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:2746748/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:5126498/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:3600789/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCGCACCATAAAGCCGCTTCTTGGACGGGTAGGTGTGAATCAAAAATAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  1:5104794/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGGGGTTTTCCGGGGCTCACCCTCACTCCGCTTGGTGAACGGGTAGGTGTGGAGCGAAAAGTAGTAGGCACCCAGGAGTATGAGGaa                                                        >  1:3739816/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTTTTCAGGGGCTCACCAACAAGCCGCGTCTTGAAAGGTTAGGTGTGAATCAAAAAAAAGAAGGCAAACACGAGTATGAGGaa                                                        >  1:3196619/1‑140 (MQ=255)

                                                                                      cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTTTTCAGGGGCTCACCATCAAGCCCCTTGTTTAACGGTTAGGTGTGAATAAAAAACAAAAAGGCAAACACGAGTATGAGGAAt                                                       >  1:216613/1‑140 (MQ=255)

                                                                                      cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCGTGTTGTTGAGGGGCTCACCATCAAGCCGCTTGTTGAACGGTTATGTGTGGATGAAAAAAAATAAGGCAACCACGAGTATGTGGAAt                                                       >  1:4667518/1‑140 (MQ=255)

                                                                                          ggACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTGCCTGGTTGTTCAGGGGCTCACCATAAAGCCGCGTCTTGAAAGGTTAGGTGTGTATCAAAAAGAATAAGGCAACCAGGAGTATTAGGAAttggt                                                   >  1:4823060/1‑138 (MQ=255)

                                                                                            ccgcgcaGACCTGCTGGTCGTCGCCTTCAGTGTCGGTTTGATGGCCCGTGTTCTTCACGGCCTCACCATCACGCCGCTTCTGGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgcttg                                                 <  2:3007829/134‑1 (MQ=255)

                                                                                            aCGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgcttg                                                 <  2:1163113/140‑1 (MQ=255)

                                                                                              gTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGTACGGTGAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCa                                               >  2:3407128/1‑140 (MQ=255)

                                                                                              gTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCAACATCAAGCCGCTTCTTGAAAGGGTAGGTGTGAAACAAAAATAAGAAGGCAACCAGGAGTATGAGGAATTGCTAGCa                                               >  2:4931519/1‑140 (MQ=255)

                                                                                               tGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGTGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaa                                              <  2:1701253/140‑1 (MQ=255)

                                                                                               tGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaa                                              <  1:2644382/140‑1 (MQ=255)

                                                                                               tGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaa                                              <  2:4118374/140‑1 (MQ=255)

                                                                                               tGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaa                                              <  2:2565602/140‑1 (MQ=255)

                                                                                               ggAAGACCTTCGGGTGTTCGCCTTCCGTGTCGTTTTGTTTTCCCTGGGTGTTCCGGCGCTCACCATCACGCCGCGTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaa                                              <  2:3981725/139‑1 (MQ=255)

                                                                                                gAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTGTTAAGGGGCTCACCATCAAGCCCCTTCTTGAACGGTTATGTTTGAATAAAAAAGAAGAACGCAAACAGGAGAATTAGGAATTTCTTGCaaa                                             >  2:611244/1‑140 (MQ=255)

                                                                                                    aCATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGa                                         <  1:3600789/140‑1 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  2:887770/140‑1 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTTCCTTGTTGTTGAGGGGCTCACCAACAAGCCGCGTGTTGTACGGGTTGGGGGGAATCAAAAAATAGAAGGCAAACAAGAGTTTGAGGAATTGGTTGGAAAATGACAta                                     >  1:2290122/1‑139 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTGAGGGGCTCACCATCAATCCGCGTGGTGAACGGTTTGGTGTGAATCAAAAATAATAATGCAACCAGGAGTATTATGAATTGGTTGCAAAAGGACATc                                     >  1:5066982/1‑140 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAACCCGCTTCATGAACCGTTAGGTGTGAATAAAAAAGAAGAAGGCAACCAGGAGTAATAGGAAATTCTTGCAAAAGGACaac                                     >  2:679418/1‑138 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGGTTTCCCTTGGTGTTGAGGGGGTCACCATCAAGCCGCTTGTTGAACGGGTAGGTGTGAATCCAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCGTGCCAAAggccctc                                     >  1:3539722/1‑135 (MQ=255)

                                                                                                            gTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGt                                                                                                    <  1:1299882/73‑1 (MQ=255)

                                                                                                                 cGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGt                                                                <  1:106756/104‑1 (MQ=255)

                                                                                                                  gCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTGAGGGGCTCACCAACAAGCCGCGTGTTGAAAGGTTAGGTGTGAATCAAAAATAATAAGGCAAACACGAGTATTAGGAAATTCGTTCAAAAGGACAAACCTTAGAAAc                           >  1:1948516/1‑140 (MQ=255)

                                                                                                                      tCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCACGAGTATGTGGAATTGCTTGCAAAAGGACATCGCGTAGAAAACGcc                       >  2:2651703/1‑140 (MQ=255)

                                                                                                                        aGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCAt                     <  2:3113610/140‑1 (MQ=255)

                                                                                                                             cGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAaag                <  2:4543222/140‑1 (MQ=255)

                                                                                                                              gttttgttttCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    >  2:3791095/1‑87 (MQ=255)

                                                                                                                                      ttCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGc       >  2:1100474/1‑140 (MQ=255)

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`