BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	595,180	G→T	7.0%	L22F (TTG→TTT)	EO946_RS03130 →	MMPL family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	595,180	0	G	T	7.0%	702.0 / 13.3	283	L22F (TTG→TTT)	EO946_RS03130	MMPL family transporter
Reads supporting (aligned to +/- strand): ref base G (105/151); new base T (25/2); total (130/153)
Fisher's exact test for biased strand distribution p-value = 1.37e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GAGAAACACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTA  >  NZ_CP034943/595041‑595319

gagaAACACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCATAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTg                                                                                                                                               >  1:2587063/1‑140 (MQ=255)

   aaaCACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAgt                                                                                                                                            <  1:1719458/140‑1 (MQ=255)

   aaaCACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAgt                                                                                                                                            <  1:5119904/140‑1 (MQ=255)

   aaaCACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTGTGAgt                                                                                                                                            >  2:2386530/1‑140 (MQ=255)

   aaaCACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAgt                                                                                                                                            >  2:406745/1‑140 (MQ=255)

        cTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAgtttgtt                                                                                                                                       >  2:4186380/1‑140 (MQ=255)

           aaaTTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGttttg                                                                                                                                    >  1:267258/1‑139 (MQ=255)

            aaTTCTATCATCTAGATATTCACCATTCTAAAAAGCTAATTTAAAGGAGGAGATTCTGCAGTATGAGAGCTATCTTCAAATTAAAGTGTGCAATTGCATCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTtct                                                                                                                                   <  2:801118/140‑1 (MQ=255)

              ttCTATCATAGATATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTcc                                                                                                                                 <  2:4574351/140‑1 (MQ=255)

                 tATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCCAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGaa                                                                                                                              >  2:5195418/1‑140 (MQ=255)

                 tATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGaa                                                                                                                              >  2:2276890/1‑140 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCACTTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATCCGaaat                                                                                                                            >  2:3180511/1‑138 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTATCACTTACCGTCGTGTTTAGTTTGTTTTATACGaaat                                                                                                                            >  1:3834058/1‑138 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATGCGAACt                                                                                                                            >  1:1306063/1‑140 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACt                                                                                                                            >  2:583742/1‑140 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACt                                                                                                                            >  2:4483021/1‑140 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTTGTTTTTTTTCTCCGAACt                                                                                                                            >  2:380579/1‑140 (MQ=255)

                   tCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCACCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACt                                                                                                                            >  2:2881631/1‑140 (MQ=255)

                      tAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTaa                                                                                                                         <  1:2755066/140‑1 (MQ=255)

                       cgagaTATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTGATATTAGCACTTACCGTCGTTTTGAGTGTGTTTTCTCCCAACTGAAc                                                                                                                        >  2:3884042/2‑140 (MQ=255)

                         agaTATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTGTCCGAACTTAACgg                                                                                                                      >  2:210224/1‑140 (MQ=255)

                             atTCACCATTCTAAAAAGCACATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTGATATTCGCACTTACCGTCGTGGTGGGGTTGTTTTGGCCGCACGTAACGGGGcg                                                                                                                  >  2:2750812/1‑139 (MQ=255)

                             atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGcc                                                                                                                  >  2:3453800/1‑139 (MQ=255)

                             atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACATAACCGAACt                                                                                                                  >  1:2120112/1‑140 (MQ=255)

                             atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTTGTTTTTTTTTTAAGAACTTAACCGAGCt                                                                                                                  >  1:3495176/1‑140 (MQ=255)

                             atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTCCGAACTTAACGGAGCt                                                                                                                  >  1:1192879/1‑140 (MQ=255)

                              ttCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTg                                                                                                                 >  2:748363/1‑140 (MQ=255)

                                cACCATTCTAAAAAGCAAATTTAAAGGAGGATATTATGCAGAATTAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                               <  2:2011109/140‑1 (MQ=255)

                                cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                               <  1:369472/140‑1 (MQ=255)

                                cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGACAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                               <  1:2935242/140‑1 (MQ=255)

                                   cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCaa                                                                                                            <  1:2458628/140‑1 (MQ=255)

                                   cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATACGAACTTAAAGGAGCTGGCCaa                                                                                                            >  2:2022877/1‑140 (MQ=255)

                                     ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTGTACGAACATAAAGGAGCGGGCCaaac                                                                                                          >  2:1003698/1‑138 (MQ=255)

                                     ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGACCTTAACGGAGCTGGCCAATc                                                                                                          >  2:4794744/1‑140 (MQ=255)

                                      tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTTTTTTATGCGAAATTAAAGGAGCTGGCCAATCa                                                                                                         >  1:3793979/1‑140 (MQ=255)

                                        tAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                       <  1:5035051/140‑1 (MQ=255)

                                        tAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                       <  1:748363/140‑1 (MQ=255)

                                             aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                  <  1:4545522/140‑1 (MQ=255)

                                             aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                  <  2:4611857/140‑1 (MQ=255)

                                             aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                  <  2:3983380/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  2:442341/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  1:2303782/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  2:1790333/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  1:4616560/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  1:3180511/140‑1 (MQ=255)

                                              gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  2:4646532/140‑1 (MQ=255)

                                               cAAATTTAAATGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGAGGGCAATTGCAGCGGTTATATTAGCACTTAACGTCGTTTTTAGTTTTTTTTATAAGAAATTAAACGACCTTGCCAATCAAAAAGGGcc                                                                                                >  2:3604937/1‑139 (MQ=255)

                                                        aggcggAGATTCTGCAGAATGAGAGCAATCATCTAATTCAAGTGGGCAATTGCAGCGGTTATATGAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCt                                                                                       <  2:4019547/140‑1 (MQ=255)

                                                        aggacgAGATTCTGCAGAATGAGAGCATTCATATAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCGGGCCAATCAATAGGGGCAGGCCCAGCt                                                                                       <  2:5067952/140‑1 (MQ=255)

                                                         ggaggaGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCtt                                                                                      >  2:495873/1‑140 (MQ=255)

                                                             gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTACGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGTCCCAGCTTCCtc                                                                                  >  2:371191/1‑139 (MQ=255)

                                                             gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTTTTTTTTCCGCACATAAAGGAGCGGGCCCAACAAAAAGGGCCGGCCCAGCTTccag                                                                                  >  1:2016996/1‑138 (MQ=255)

                                                             gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTGATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTATCCGAACTTAACCGATCAGGCCAAACAAAATGGGCAGGCCCAGCTTCCTg                                                                                  >  2:3766818/1‑140 (MQ=255)

                                                                aTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTTTTTTCTACGAACCTAACGGAGCTTTCCAATCCAAACTGGCATGCCCAGCttacatcag                                                                               >  2:1112444/1‑133 (MQ=255)

                                                                   cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1016079/140‑1 (MQ=255)

                                                                    tGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTCGCCACACAAAACGGGCAGGCCCAGCATCCTGCCGATGc                                                                           >  2:470888/1‑140 (MQ=255)

                                                                         aaTGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGGTTTTTTTTTTTACCAACATAAACGAGCTTGCCAATCAAAAGGGGCCCGCCCCGCGTGCTGCAGATGCTGttt                                                                      >  1:4846999/1‑140 (MQ=255)

                                                                           tGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTATCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCAGCCGATGCTGtttga                                                                    >  2:31051/1‑138 (MQ=255)

                                                                              gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTGTTTTGTACGAAATTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTGCTGCAGATGCTGTTTAAGaa                                                                 >  2:1686963/1‑140 (MQ=255)

                                                                                   atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTACGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTAAGaaacatc                                                            >  2:3252255/1‑136 (MQ=255)

                                                                                   atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTACGAAATTAACGGGGCTGGCCCATCAAAAGGGGCAGGCCCAGCTTCCTTCAGATGCTGTTTAACAAAGAG‑c                                                           >  2:965055/1‑140 (MQ=255)

                                                                                   atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGGGTTTTTTTTGTCCGAACTTAACGGATCGGGGCAATCAAAAGGGGGAGGCCCACCATGCAGCAGATTCTTGTTCAGAAATAG‑c                                                           >  1:4894355/1‑140 (MQ=255)

                                                                                       tcaAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTTTTTTGTACGAACTTAAACGAGCGGGGCCAAAAAAAAGGGCCCGCCCACCTTCCTGCCGATTCTTGTTGAGAAAGAG‑C‑aaaa                                                      >  1:632268/1‑139 (MQ=255)

                                                                                                   gggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTaaaa                                          <  2:4097609/140‑1 (MQ=255)

                                                                                                    ggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTGTCCGAACTTAAAGGAGCGGGCCAAACAAAATGGGCACGCCCACCTTCCAGCAGATGCTGTTTGAGAAACAG‑CAAAAAGCAATTTTaaaa                                          >  1:4315814/1‑140 (MQ=255)

                                                                                                          tGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCgg                                   <  2:1543955/140‑1 (MQ=255)

                                                                                                          tGCAGAGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCgg                                   <  2:1015590/140‑1 (MQ=255)

                                                                                                            cAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAACAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGga                                 <  2:2587063/140‑1 (MQ=255)

                                                                                                               cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGagaa                              <  1:4201781/140‑1 (MQ=255)

                                                                                                               cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGagaa                              <  2:2785733/140‑1 (MQ=255)

                                                                                                               cGGTTATATGAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGagaa                              <  1:31051/140‑1 (MQ=255)

                                                                                                                   tataTTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCACCTTCCTTCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACa                          >  2:5130388/1‑140 (MQ=255)

                                                                                                                    atatTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACTTAACGGAGCTGGCCCAACAAAATGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAACACaa                         >  2:3641043/1‑140 (MQ=255)

                                                                                                                      atTAGCACTTACCGTCGTGTTGAGTTTTTTTTCTACGAACTTAAAGGAGCTGGCCAATCAAAACGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTAAGAAAAAG‑C‑AAAAGCCATATTAAAAAAAGCGGGAGAATACaaaa                       >  2:3339260/1‑138 (MQ=255)

                                                                                                                      atTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCAGCAGATGCTGTTTCAGAAACAG‑C‑AAAAGCCCTTTTAAAAAAATCGGGAGAAGACcata                       >  2:2656798/1‑140 (MQ=255)

                                                                                                                        tcgaaCTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                     <  1:3989330/136‑1 (MQ=255)

                                                                                                                        tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                     <  2:3834058/140‑1 (MQ=255)

                                                                                                                        tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                     <  1:4103787/140‑1 (MQ=255)

                                                                                                                        tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                     <  2:1306063/140‑1 (MQ=255)

                                                                                                                          gCACTTACCGTCGTTTTGGGTTTGTTTTGAACGTACGTAACGGTGCTGGCCCAACAAAAGGGGGTGGGCCCACTTCCTGCCGATGCTGTTTGGGAAATAG‑C‑ACATGCCCTTTTAAAACCAGCGGGAGAAGACAATAAttg                   >  1:1007568/1‑139 (MQ=255)

                                                                                                                           cACTTACCGTCGTTTTGAGTTTGTTTTGTCCGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATTCTGTTTCATAAAGAT‑C‑AAATTGCATTTTAAAAAAAGCGGGTGAAGACAATAATTCg                  >  2:3497061/1‑140 (MQ=255)

                                                                                                                           cACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCGGGCCAATCAAAAGGGGCAGGCCCAGCGTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCCATTTAAAAAAAGCGGGAGAAGACaaaaaatag                  >  2:4997118/1‑136 (MQ=255)

                                                                                                                             cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                <  2:1587713/140‑1 (MQ=255)

                                                                                                                             cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                <  1:1835015/140‑1 (MQ=255)

                                                                                                                             cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                <  2:1223241/140‑1 (MQ=255)

                                                                                                                                aCCGTCTTTTTTAGTTTTTTTTATCCGCACTTAACGGAGCTGGCCAATCAAAATGGGCAGGCCCAGCGTCCGGCCTATGGTGTTTACGCAAGAGCC‑AAATG‑CATTTTAAAAATAGGGGGAGAATACGATAATTGGATCAg             >  2:630411/1‑140 (MQ=255)

                                                                                                                                 ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt            <  2:4834608/140‑1 (MQ=255)

                                                                                                                                 ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt            <  1:1573687/140‑1 (MQ=255)

                                                                                                                                 ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt            <  1:1917297/140‑1 (MQ=255)

                                                                                                                                   gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt          <  1:1476338/140‑1 (MQ=255)

                                                                                                                                   gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt          <  2:247331/140‑1 (MQ=255)

                                                                                                                                   gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt          <  2:4585794/140‑1 (MQ=255)

                                                                                                                                   gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt          <  2:783391/140‑1 (MQ=255)

                                                                                                                                   gtcgtTTTGAGTTTGTGTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt          <  1:56440/140‑1 (MQ=255)

                                                                                                                                      gtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGt       <  2:4315814/140‑1 (MQ=255)

                                                                                                                                        tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt     <  1:3049253/140‑1 (MQ=255)

                                                                                                                                        tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt     <  1:3264019/140‑1 (MQ=255)

                                                                                                                                        tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt     <  1:406745/140‑1 (MQ=255)

                                                                                                                                        tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt     <  2:3213333/140‑1 (MQ=255)

                                                                                                                                         ttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCtt    <  1:2198459/140‑1 (MQ=255)

GAGAAACACTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAG‑C‑AAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTA  >  NZ_CP034943/595041‑595319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`