Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,531 A→C 6.3% intergenic (+209/‑95) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5310AC 6.3% 1161.4 / 18.8 473intergenic (+209/‑95)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (160/264);  new base C (45/2);  total (206/267)
Fisher's exact test for biased strand distribution p-value = 1.13e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

GATCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAT  >  NZ_CP034943/112395‑112670
                                                                                                                                        |                                                                                                                                           
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCcgc                                                                                                                                         >  1:911080/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCcgc                                                                                                                                         >  1:2300658/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCcgc                                                                                                                                         >  1:1488907/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  1:1060812/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:1490751/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:1930057/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  1:2378407/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  1:221746/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:2677490/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:3214957/1‑140 (MQ=255)
 aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGGATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:2122056/1‑140 (MQ=255)
  tCCATGAGCCGTGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCTCTATTCAAAGGCGGCCGCCGCCGTTAGAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:3473727/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGTAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCTCCGTTCCAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  2:1818701/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:1573132/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  2:2428552/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:2084555/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:1412394/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:1335056/140‑1 (MQ=255)
  tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:1167240/140‑1 (MQ=255)
      tGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGt                                                                                                                                    >  1:992/1‑140 (MQ=255)
         gCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTa                                                                                                                                 >  2:489400/1‑140 (MQ=255)
         gCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTa                                                                                                                                 >  2:116495/1‑140 (MQ=255)
          ccGGGTGTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTaa                                                                                                                                >  2:3476346/1‑140 (MQ=255)
             ggTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCg                                                                                                                             >  1:2012440/1‑140 (MQ=255)
                      aGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCa                                                                                                                    <  2:2747947/140‑1 (MQ=255)
                      aGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCa                                                                                                                    <  1:1032789/140‑1 (MQ=255)
                        tGCGCCCTTAAGACCTCTGAAGAACAGTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTCCAGGCTGAAGTTGGGAGAGCAGACGTCTGCTTTTCAAACTGAGTGGAACCGCGCGGTTTAAGCGTCTCTggcctg                                                                                                                  >  2:4207508/1‑135 (MQ=255)
                         gcgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGGATTCAAATGCGGCCGCCGCCGTTACAGGCTGACGTTGGGACAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCagtt                                                                                                                 >  2:828777/1‑137 (MQ=255)
                          cgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAg                                                                                                                               <  2:69941/125‑1 (MQ=255)
                          cgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAg                                                                                                                               >  1:69941/1‑125 (MQ=255)
                          cgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGtt                                                                                                                >  2:341746/1‑140 (MQ=255)
                                    aCCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCaga                                                                                                      >  2:108350/1‑140 (MQ=255)
                                      ctctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAgg                                                                                                    >  2:3572206/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTCCCGGCTGAAGTTGGGAGAGCCGAAGTCTGCTTTTCAAACCGAGTGGACCCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGACgc                                                                                                   >  2:2333174/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGATgc                                                                                                   >  1:4030717/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCa                                                                                                                    <  1:3219503/123‑1 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  2:4147207/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  2:2905991/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   <  1:2535093/140‑1 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   <  2:1253987/140‑1 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  1:4144358/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGGTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  1:2988221/1‑140 (MQ=255)
                                       tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGATGAAGTTGGGAGAGCCGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGCTTTAAGCGTCACTGTCATGTTTTCATGCAGAGgc                                                                                                   >  2:953165/1‑140 (MQ=255)
                                         tGACGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCCAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCATAGgcgc                                                                                                 >  2:4059173/1‑140 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTTGGAGAGCAGAAGTCTGCTTTTCAAAAAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  2:3832713/1‑140 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:50659/1‑140 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAg                                                                                                                               >  1:1844729/1‑110 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAg                                                                                                                               <  2:1844729/110‑1 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAg                                                                                                                               >  1:2671764/1‑110 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGtct                                                                                                                          <  1:3774426/115‑1 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:393487/1‑140 (MQ=255)
                                         tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:151962/1‑140 (MQ=255)
                                                cATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCttttttt                                                                                          >  1:800533/1‑140 (MQ=255)
                                                     ttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTgg                                                                                     >  2:2596645/1‑140 (MQ=255)
                                                        gAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTTCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTg                                                                                  >  1:3030334/1‑140 (MQ=255)
                                                              aGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAgg                                                                            <  1:100112/140‑1 (MQ=255)
                                                                 aTTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                         <  2:393487/140‑1 (MQ=255)
                                                                 aTTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                         <  2:1332633/140‑1 (MQ=255)
                                                                      aaGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCaaa                                                                    <  1:339754/140‑1 (MQ=255)
                                                                          cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTGTTTTATTGTGTGAAGAGGAAATCAAATtga                                                                >  1:2061002/1‑140 (MQ=255)
                                                                           ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                               <  1:3344966/140‑1 (MQ=255)
                                                                            gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        >  1:1777248/1‑66 (MQ=255)
                                                                            gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:443233/66‑1 (MQ=255)
                                                                            gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGTGGAAATCAAATAgaga                                                              >  2:2240425/1‑140 (MQ=255)
                                                                            gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAtaga                                                              >  1:4179286/1‑140 (MQ=255)
                                                                            gcagccgccgTTACAGGCTCAAGTTGGGAGAGCAGAAGTATGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGCAATCAAAAAgaga                                                              >  2:2491401/4‑140 (MQ=255)
                                                                                           ggatGAAGTTTGTTGAGCAGATGTCTGCTTTTCAAACAGAGTCGAACTGCGCTGATTCAGCGTCTCTGTCATTTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAACTGGCGGGAAGCAt                                               <  1:561408/137‑1 (MQ=255)
                                                                                           ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGTTTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTAATGCTGAGGCGGTTTTTTTATTGGGTGATGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                               <  1:3127436/140‑1 (MQ=255)
                                                                                           ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                               <  2:1371683/140‑1 (MQ=255)
                                                                                            gCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTCGAGGCAATCAAATAGAGAAGTTGGGGGAAGCAtg                                              >  2:1439649/1‑140 (MQ=255)
                                                                                                aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGCAATACAATATAGAAGTTGGGGGGAGCATGTGtt                                          >  1:1099322/1‑140 (MQ=255)
                                                                                                aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAAAGAGAAGTGGGGGGAAGCATGTGtt                                          >  2:174930/1‑140 (MQ=255)
                                                                                                      ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTAATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAg                                    <  1:3856263/140‑1 (MQ=255)
                                                                                                       gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTGTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                   <  2:3359048/140‑1 (MQ=255)
                                                                                                       gagaGCAGAAGTCTGCTTGTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                   <  2:2284695/140‑1 (MQ=255)
                                                                                                          agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATg                                                                                                                  >  1:195191/1‑58 (MQ=255)
                                                                                                          agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGTAGAGGAAATCAAAAAGAGAAGTGGGGGGGACCAAGTGTTTTTTATAATg                                >  1:2701334/1‑140 (MQ=255)
                                                                                                          agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCAAATAGAGAAGGTGGGGGAAGCATGTGTTTTTTATAAtt                                >  1:2579789/1‑139 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:354399/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:3157147/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:2464561/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:2866210/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:1200655/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:2775727/140‑1 (MQ=255)
                                                                                                                   cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:3296853/140‑1 (MQ=255)
                                                                                                                    tGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTGTTTAGAATGCTCAAagaaga                     <  2:3309189/140‑1 (MQ=255)
                                                                                                                     gattttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTAATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:3798280/138‑1 (MQ=255)
                                                                                                                     gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:2028424/140‑1 (MQ=255)
                                                                                                                     gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:800533/140‑1 (MQ=255)
                                                                                                                     gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:1644239/140‑1 (MQ=255)
                                                                                                                     gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:3797721/140‑1 (MQ=255)
                                                                                                                     gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCATCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:555182/140‑1 (MQ=255)
                                                                                                                      cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGACTAGGGAATCAAATTGAGAAGGGGGGGGAACCATGTGTTTTTTATAATGCTGCAAGAAGAc                    >  1:2156561/1‑140 (MQ=255)
                                                                                                                             caCAGAGTGGAACCGCGCGGTTGAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGCGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  2:4030717/139‑1 (MQ=255)
                                                                                                                             aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  1:1261004/140‑1 (MQ=255)
                                                                                                                             aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAGCAAAGAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  2:2548002/140‑1 (MQ=255)
                                                                                                                               cAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACa           <  2:3742570/140‑1 (MQ=255)
                                                                                                                                   gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAgtgt       <  2:1615237/140‑1 (MQ=255)
                                                                                                                                   gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAgtgt       <  2:1045914/140‑1 (MQ=255)
                                                                                                                                        aCCGAGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGTGTCGCTTTTTTTATTGGGTGAAGAGTAAATCAAATAGAGAAGTGGGGGGAAGAATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAt  <  1:874093/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GATCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAT  >  NZ_CP034943/112395‑112670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: