Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 485,075 C→A 7.4% H453N (CAC→AAC)  kimA → potassium transporter KimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943485,0750CA 7.4% 670.5 / 3.7 267H453N (CAC→AAC) kimApotassium transporter KimA
Reads supporting (aligned to +/- strand):  ref base C (94/139);  new base A (31/2);  total (125/142)
Fisher's exact test for biased strand distribution p-value = 1.53e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

ATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAATCGATTCAATACGCAAAATCA  >  NZ_CP034943/484940‑485206
                                                                                                                                       |                                                                                                                                   
aTGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTa                                                                                                                                 <  2:2634115/140‑1 (MQ=255)
 tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTac                                                                                                                                >  2:2964629/1‑140 (MQ=255)
 tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTac                                                                                                                                >  2:478288/1‑140 (MQ=255)
 tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTac                                                                                                                                <  1:3616834/140‑1 (MQ=255)
 tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTGATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTac                                                                                                                                <  2:2413863/140‑1 (MQ=255)
  gCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTaca                                                                                                                               <  2:1709901/140‑1 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           <  1:2548446/140‑1 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           <  1:2460865/140‑1 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           <  2:388536/140‑1 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           <  1:1936155/140‑1 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           >  2:332948/1‑140 (MQ=255)
      aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           >  2:2345350/1‑140 (MQ=255)
      aTCAACTACTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                                           <  1:4137321/140‑1 (MQ=255)
         aaCTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGt                                                                                                                        <  2:1379996/140‑1 (MQ=255)
           cTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCg                                                                                                                      <  2:568233/140‑1 (MQ=255)
           cTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCg                                                                                                                      <  2:2673910/140‑1 (MQ=255)
              cTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTg                                                                                                                   >  2:4095754/1‑140 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  1:928442/140‑1 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  1:589649/140‑1 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  2:2053957/140‑1 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  2:3049472/140‑1 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  1:1634487/140‑1 (MQ=255)
                gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAAGGTTGTATGGCCGGTGTTAATCTTTATGCCTATCGTCGTTTTTGTGTTTTTTTCGATTAAAAATCACTAAACAGCTGTCGGTGaa                                                                                                                 >  1:209934/1‑140 (MQ=255)
                   gcgcTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTAGGTGAACAg                                                                                                              >  2:861122/1‑140 (MQ=255)
                   gcgcTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACTACACAGCAGTCGGTGAACAg                                                                                                              >  2:4053293/1‑140 (MQ=255)
                   gcgcTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTGAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACACCTGTCGGGGAACAg                                                                                                              >  2:2202957/1‑140 (MQ=255)
                      cTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTTTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATAGTCGTGTTTCTTTTTTTTGCGATTTAAAATAACTAAAAACCTGTCGGTGAACATCtt                                                                                                           >  1:3176618/1‑140 (MQ=255)
                       tttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTc                                                                                                          <  2:905926/140‑1 (MQ=255)
                       tttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTc                                                                                                          <  1:1094572/140‑1 (MQ=255)
                              tCGTTTATGGTTCTGTCGAGCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGt                                                                                                   <  2:2880280/140‑1 (MQ=255)
                               cGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTa                                                                                                  <  1:572580/140‑1 (MQ=255)
                                          cTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGcc                                                                                       >  2:3790169/1‑140 (MQ=255)
                                          cTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATAGTAGACAAAGAGcc                                                                                       >  1:4108487/1‑140 (MQ=255)
                                           tGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCa                                                                                      >  1:1027498/1‑140 (MQ=255)
                                            gTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCag                                                                                     <  1:843795/140‑1 (MQ=255)
                                               gATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCagaag                                                                                  <  1:1761415/140‑1 (MQ=255)
                                                  ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTGTGTTTTTTGGGATTAAAAATCACAAAACAACTGTCGGTGAACAGCTTCGCCTGGTAGAAAAAGAGCCcgacggaa                                                                               >  1:3245049/1‑135 (MQ=255)
                                                  ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTATACAAAGAGCCAGAAGaaa                                                                               >  1:6439/1‑140 (MQ=255)
                                                  ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAATATCCAGAAGaaa                                                                               >  1:3128260/1‑140 (MQ=255)
                                                  ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGaaa                                                                               >  2:1927036/1‑140 (MQ=255)
                                                         gtgACGAAGTTTTATGTTGTATGGCCTGTGTTAATCTTTATTCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAgg                                                                        <  1:2255125/140‑1 (MQ=255)
                                                         gtgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAgg                                                                        <  1:2089987/140‑1 (MQ=255)
                                                         gtgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTAGCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAgg                                                                        <  1:2942490/140‑1 (MQ=255)
                                                          tgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAGGGc                                                                       <  1:1894853/140‑1 (MQ=255)
                                                          tgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGc                                                                       <  2:762340/140‑1 (MQ=255)
                                                          tgAAGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGc                                                                       <  2:1554517/140‑1 (MQ=255)
                                                           gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                                                      <  1:4010886/140‑1 (MQ=255)
                                                           gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                                                      <  2:3080010/140‑1 (MQ=255)
                                                           gACGAAGTTTAATGTTGTAGGGCCTGTGTTAATCTGTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                                                      <  1:2385732/140‑1 (MQ=255)
                                                            aCGAAGTTTAATGTTTTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCAc                                                                     <  2:279498/140‑1 (MQ=255)
                                                            aCGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAAACAAAGGCAc                                                                     <  1:967581/140‑1 (MQ=255)
                                                               aaGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGt                                                                  <  1:3691133/140‑1 (MQ=255)
                                                                aGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAGAACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGtt                                                                 >  2:421999/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATCAATAAACACCTGTAGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATAAAAGGCACCGTTGTTATTGTTCCt                                                     >  1:212162/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATAAATAAACAGCTGTCGGTGAACAGCTTCGCATCGTAGAAAAACAGCCAGAAGAAATAAAAGGCACGGTTGTGATTGTGCCt                                                     >  1:2657319/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATCACTACACAGCTGTCGGTGAAAAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTTATTTTGCCt                                                     >  1:3062417/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATCACAACACAGCTGTCGGTGAACAGCTTAGCATCGTAGACAAAGAGCCAGAAGAAATCAAATGCACGGTTGTGATTGTGCCt                                                     >  1:1533617/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATAACTACACAGCTGTCGGTGAAAAGCTTCGCATCGTAGAAAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTTCCt                                                     >  1:3864635/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATAAAAGGCACGGTTGTTATTGTGCCt                                                     >  1:3377662/1‑140 (MQ=255)
                                                                            tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTTCTGTTTTTTGCGATTAAAAATAACTACACATCTGTAGGTGAAAAGCTTCGCATAGTAGACAAAAAGACAGAAGAAATAAAAGGAACGGTTTTGATTGTGCCt                                                     >  1:242834/1‑140 (MQ=255)
                                                                              tGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATAACTAAACAGCTGTCGGTGAAAAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTTTGATtctgtgtct                                                   >  2:41731/1‑135 (MQ=255)
                                                                                 ccTGTGTTAATCTTTATGCATATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGc                                                <  2:3352382/140‑1 (MQ=255)
                                                                                  cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                                               <  2:3188147/140‑1 (MQ=255)
                                                                                  cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                                               <  2:1078685/140‑1 (MQ=255)
                                                                                  cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                                               <  1:938864/140‑1 (MQ=255)
                                                                                  cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                                               <  1:280365/140‑1 (MQ=255)
                                                                                  cTGTGCTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                                               <  1:2503939/140‑1 (MQ=255)
                                                                                    gtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 <  1:3455863/72‑1 (MQ=255)
                                                                                    gtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                                                 >  2:3455863/1‑72 (MQ=255)
                                                                                    gtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGc                                                                       >  2:3404831/1‑114 (MQ=255)
                                                                                    gtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCgg                                             <  2:3691116/140‑1 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACCGTTGTGATTGTGCCTGTATCCGgt                                            >  1:2026416/1‑140 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAAAAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            >  2:212847/1‑140 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            <  1:1724835/140‑1 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            <  1:3762545/140‑1 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            <  2:3947954/140‑1 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            <  1:2944862/140‑1 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCCGTGAACAGCTTCGCATAGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCGGTAGCCGgt                                            >  1:50979/1‑140 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                                            >  1:4219700/1‑140 (MQ=255)
                                                                                     tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACAACACACATGTCGGTGAACAGCTTCGCATCGTAGACAAAGATCCAGAACAAATCAAAGGACAGGTTGTTTATGTGCCTGTACCCCgt                                            >  1:3351708/1‑140 (MQ=255)
                                                                                          aTCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAAAAACAACACACCTGTCGGTGAAAAGCTTCGCATCGTAGACAAAAAGCCAGAAGAAAAAAAAGGCACCGTTGTGATTGTGCCTGTAGCCGGTGTtaa                                       >  1:3187208/1‑139 (MQ=255)
                                                                                           tCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATCACTACACAGCGGTCGGTGAACAGCTTCGCCTCGTAGACAAAGAGCCCGTAGAAATCAAAGGCACGGTTGTGATTGTTCCTGTAGCCGGTGTtact                                      >  1:3662419/1‑140 (MQ=255)
                                                                                            cTTTATGCCAATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGGTGTGATTGTGCCTGTAGCCGGTGTtacta                                     <  2:202203/140‑1 (MQ=255)
                                                                                              ttATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACaa                                                                                             <  2:450539/82‑1 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTTCTGTTTTTTTCGGTTAAAAATCACTACACAGCAGTCGGTGAAAAGCTTAGCATCCTAGAAAAAAAGCCACAAGAAATCAAAAGCACCGTTGTGATTTTTCCAGTATCCGGTTTtaataacc                                  >  1:2738650/1‑137 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTTAAAATAACTACACAGCTGTCGGGGAACAGCTTCGCATCGTAGACCAAGTGCCAGAAGAAATCAAACGCCCGGTTGTGATTGTTCCTGTAGCCGGTGTTACTACcg                                  >  1:1207244/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg                                  >  1:2852403/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATAACAAAACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAAACAAACGCACCGTTGTGATTTTGCCTGTAGCCGGTGTTACTAAcg                                  >  1:1395063/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCAGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg                                  >  2:1138338/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTAAACAGCTGTCGGTGAACAGCGTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg                                  >  1:2983404/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAAATACACAGCTGTAGGTGAAAACCTTATCATCGTAGACAAATACCCAGAAGAAATAAAAGGCACGGATGTGAATGTGCCAGTAGCCGGTGTTAATACcg                                  >  2:958892/1‑140 (MQ=255)
                                                                                               tATGCCTATCGTCGTTTTGCTGTTTTTTGAGATTAAAAAAAACAACACAGCGGGAGGTGAAAATATTCGCATCCGAGAAAAACAGCCAGAACAAATAAAAAGCAACGTTTTTATTGTGGCTGTAACCGGTGTTACTACcg                                  >  1:2285729/1‑140 (MQ=255)
                                                                                                      aTCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCa                           <  1:4193908/140‑1 (MQ=255)
                                                                                                      aTCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCa                           <  1:4206528/140‑1 (MQ=255)
                                                                                                      aTCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTAACGTCGTGCa                           >  1:2879282/1‑140 (MQ=255)
                                                                                                      aTCGTCGTGTTGCTGTTTTTTTCGATTAAAAATCACTACACAGCTGTCCGTTAACAACATCGCATCGTAGACAAAGAGCCACAAGAAATCAAAAGCACCGTTGTGATTTTTCCAGTAGCCCGTGTTAAAACCCTCGTGCa                           >  1:1788500/1‑140 (MQ=255)
                                                                                                       tcgtcgTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAGGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCaa                          >  2:1049222/1‑140 (MQ=255)
                                                                                                              ttttCTGTTTTTTGCGATTAAAAATCACTAAACCGCTGTCGGTGAAAAGCTTCGCATCGTAGACAAATAGGCAGAAGAAATAAAAGGCACCGTTTTGATTTTGCCTGTAGCCGGTGTTAATAACGTCGTGCAAAAATCGa                   >  1:770672/1‑140 (MQ=255)
                                                                                                                            cGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAATCGATTCAATACGCaaaa     >  2:3137019/1‑140 (MQ=255)
                                                                                                                              aTTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAATCGATTCAATACGCAAAATc   <  1:3410328/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
ATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAATCGATTCAATACGCAAAATCA  >  NZ_CP034943/484940‑485206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: