Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,112 A→C 9.0% R42R (AGA→CGA)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1120AC 9.0% 621.7 / 3.5 260R42R (AGA→CGA) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (148/73);  new base C (2/36);  total (150/110)
Fisher's exact test for biased strand distribution p-value = 1.79e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTA  >  NZ_CP034943/548973‑549248
                                                                                                                                           |                                                                                                                                        
tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                                          <  1:2296164/140‑1 (MQ=255)
 aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                                                                         >  2:1250289/1‑140 (MQ=255)
 aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGAAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTGCAAAAg                                                                                                                                         >  1:2822607/1‑140 (MQ=255)
   gagaACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGAc                                                                                                                                       >  2:2518978/1‑140 (MQ=255)
   gagaACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGAATTCAAAAATGAATGACGCACCCTTCCAAACGAc                                                                                                                                       >  2:2459163/1‑140 (MQ=255)
      aacaacCCAAATGGATATCACGCCTTTTTTGAATCGAAAGCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTACCAAAAGACTcg                                                                                                                                    >  1:2094242/1‑139 (MQ=255)
      aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                                                    >  1:1882405/1‑140 (MQ=255)
       acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                                                                                   <  2:172145/140‑1 (MQ=255)
        caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                  >  2:2300375/1‑140 (MQ=255)
           cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                               >  2:1835831/1‑140 (MQ=255)
              aaaTGGATATCACGCCTTTGTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTGTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                            >  2:2065837/1‑140 (MQ=255)
               caTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAACGGAATGAAGCTCCCTTCCAAAAGCCTCCTGGCGAGTc                                                                                                                           <  1:3098119/139‑1 (MQ=255)
                atcgATATCACCCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTAATTCAAAGAAAACATGCATCAGGGGCGAATTAAAAAAGGAATAAAGATAACTACCAAAAGACTCATGACGAGTCa                                                                                                                          >  1:3596413/4‑140 (MQ=255)
                aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAATGGACTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                          >  1:256748/1‑140 (MQ=255)
                aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                          >  1:1087034/1‑140 (MQ=255)
                aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                          >  2:1971261/1‑140 (MQ=255)
                     tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                     >  2:2367183/1‑140 (MQ=255)
                     tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                     >  2:1497930/1‑140 (MQ=255)
                     tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                     >  2:142077/1‑140 (MQ=255)
                     tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                     >  1:884836/1‑140 (MQ=255)
                      atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGGTATTTCAAAGTAAACATGAATCAGGGGGGTATTCAAAAAGGAATCAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                    >  2:2853085/1‑140 (MQ=255)
                        cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                  >  2:273724/1‑140 (MQ=255)
                          cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                <  2:1882405/140‑1 (MQ=255)
                          cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                <  2:1202091/140‑1 (MQ=255)
                             cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                             >  1:2694106/1‑140 (MQ=255)
                             cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                             >  1:1008956/1‑140 (MQ=255)
                               tttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCa                                                                                                           >  1:1781409/1‑140 (MQ=255)
                               tttttGAATAGAAATCTGGATAGTCCGATTTATCAGCTGGTTTATCGATATTTCAAATAAAACATCCACGCGGGGCGTATAAAAAAAGGAAGGAAGCTCCCTTCCAAACGACGCCTGGCGTGTCAGCTTTCCATCCGTCa                                                                                                           <  2:2682344/140‑1 (MQ=255)
                               ttttgGAATAGAAATCTGGATAGTCCGATTTATCCGCAGCTTTATCGCTATTTCAAACAAAACATCAATCAGGGGCGTATTAAAAAAGGAATTACGCTCCCTTCCAAAAGACTCCTGGCGTGTCAGCTTTCCTTCAGTCa                                                                                                           <  2:3449171/140‑1 (MQ=255)
                                  tttaaTATAAATGTGTATATTCCTTTTTATCTGCAGCTTTATCGCTATTTCAAAGAAAAAATGAATCGGGGGCTTTTTAAAAAAGTAATTAAGCTCCTTTCCAAAAGACTCGTGGCTATCCAGCTTTCCATCAGTAAAAc                                                                                                        <  2:4088492/137‑1 (MQ=255)
                                  ttGAATAGAAATCTGGTTATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                        <  2:4121884/140‑1 (MQ=255)
                                              ttttatatTCCGGTTTATCCGCAGCTTTATCGATATTTAAAAGAAAACATGCATCAGGGGCTTATTCAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACg                                                                                            <  1:2518978/136‑1 (MQ=255)
                                              ctgttttttCCGTTTTTTCAGAAGTTTTATCGCTATTTAAAATAAAACATGCAGCCGGGGCGTATTAAAAAAGTAATGAAGCCCCCTTCCAAACGACTCCTGGCGAGTCAGCTTTCCATCCTTCAAACGACTGTAGAACg                                                                                            <  2:5821/133‑1 (MQ=255)
                                              cTGTATATTGCGCTTTATAAGCAGCTTTAGCGTTATTTCAAACAAAACATGCATCCGGGGCGTATTAAAAATGTAATGAAGCTCCCTTCCAAAACACACCTGGCGAGTCAGCTTTCCATCAGGCACACGACTGTAGAACg                                                                                            <  2:258637/140‑1 (MQ=255)
                                               tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGt                                                                                           >  2:3601223/1‑140 (MQ=255)
                                                tcatatTCCGCTTTATAAGAAGATTTATCGCTATTACAACGAAAACATGAATCAGGGGCGTATCCAAAAAGCAATGAAGCTCCATTCCAAAAGCCTCCTGGCTATTAAGCTTTCCAGCAGTCAAACGACTGTAGAACGTg                                                                                          <  2:2555732/138‑1 (MQ=255)
                                                 gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                         >  2:163564/1‑140 (MQ=255)
                                                    atTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTa                                                                                      >  1:1919536/1‑140 (MQ=255)
                                                        ctcTTTATCAGCAGATTTATCGATATTTCAAAAAAAACATGCAACCGGGGCTTATTAAAAACGCAATCAAGCTCCCTTCCAAAAGACTCCTGGCGATACAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGaa                                                                                  <  1:2666469/138‑1 (MQ=255)
                                                        cGCTTTATCCGCAGCTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAACGGAATCAAGCTCCCTTCAAAAAGACCCCTGGCGCTTCAGCTTTCCATCAGCCAAACGACTGTAGAACGTGCCTATGaa                                                                                  <  1:57369/140‑1 (MQ=255)
                                                        cGCTTTATCAGCAGCTTTATCGATATTAAAAATAAAAAATGCATCAGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGaa                                                                                  <  2:1437429/140‑1 (MQ=255)
                                                        cGCTTTACCAGCAGCTTTATCGCTATTACAAACAAAACATGAATCCGGGGCTTATAAAAAAAGCAATGACGCGCCCTACCAAACCACGCCGGGCGAGTCAGCTTTCCACCCGCCACACGACTGTAGAACGTGCCTATGaa                                                                                  <  1:1250289/140‑1 (MQ=255)
                                                               tCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCCGGGGCGTATACAAAACGCAATGACGCGCCCTTCCAAAACACCCCCGGCGAGTCAGCTTTCCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                           <  1:666670/140‑1 (MQ=255)
                                                                    agtttttTCGATATTAAAAACAAAACATGCATCTGGGGCTTATAAAAAAAGGAATGAAGCCCCCTTCCAAACCACTCCGGGCGAGTCAGCTTACCACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:2163821/137‑1 (MQ=255)
                                                                    agcTTTCTCGCTTTTACAAACAAAACATGCATCCGGGGCTTATAAAAAACGGACTGACGCCCCCTACCAAACGACCCCGGGCGAGCCAGCTTACCATCAGGCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:459851/140‑1 (MQ=255)
                                                                    agcTTTATCTCTATTTAAAAGAAAACATGAATCCGGGGCGTATTCAAAAAGGAAGGAACCACCCTTCCAAAACACTCGTGGCGAGTCTGCTTTCCAACAGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:1999818/140‑1 (MQ=255)
                                                                    agcTTTATCGCTATTTCAAACAAAACATGAATCCGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAACGACCCCTGGCGAGTCAGCTTACCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  2:884836/140‑1 (MQ=255)
                                                                    agcTTTATCGCTATTACAAACAAAACATGAAACAGGGGGTTATACAAAACGGAATCACGCTCCCTTCAAAAAGACACCTGGCTAGTAAGTTTTCCATCACACCAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:4236754/140‑1 (MQ=255)
                                                                    agcTTTATCGATTTTTCAAAGAAAACATGCATCTGGGGCGTATTCAAAAAGGAATGAAGCTCCCTACCAAACGACTCCTGGCGATTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:3838548/140‑1 (MQ=255)
                                                                    agcTTTATCGATATTTCAAATAAAACCTGCAACCGGGGCGTTTTAAAAAAGCAATGACGCCCCCTTCCAAAACACGCCGGGCGAGTCAGCTTTCCACCACCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:1445163/140‑1 (MQ=255)
                                                                    agatttATCGCTATTAAAAACAAAACATCCAACCGGGGCTTATAAAAAAAGTAATGACGCCCCCTACCAAAACCCTCCTGGCGAGACAGCTTACCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:1925601/137‑1 (MQ=255)
                                                                    agatttATCGATATTTCAAACAAAACATGCATCCGGGCCTTATACAAAAAGTATTGACGCTCCATTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                      <  1:4079180/137‑1 (MQ=255)
                                                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                     >  2:3812229/1‑140 (MQ=255)
                                                                     gatttATCTCTATTTCAAAGAAAACATGAATCAGGGGGGTATTAAAAAAGGAATGAAGGTCCCTTCCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                     <  2:2733090/138‑1 (MQ=255)
                                                                      cTTTTTCGCTATTTCAAATAAAACATGCATCCGGGGCGTATTCAAAAAGTAATGCAGCGCCCTACCAAACCACTCCTGGCGAGTCAGCTTTCCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    <  2:3068345/140‑1 (MQ=255)
                                                                      cTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGTAAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    <  2:1343835/140‑1 (MQ=255)
                                                                      cTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    >  1:3576228/1‑140 (MQ=255)
                                                                      cTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    >  1:387074/1‑140 (MQ=255)
                                                                      cTTTATCGATATTACAAATAAAACATGCATCCGGGGGGTTTACAAAAAGTAATCACCCTCCCCGCCAAACCCCCCGGGGCGATTCAGCTTCCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    <  1:3452052/140‑1 (MQ=255)
                                                                      cTTTATCGATATTAAAAACAAAACATCCATCCGGGGCGTCTACAAAAAGCAATCACGCGCCCTACCAAACCACCCCGGGCGTGACAGCTTACCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    <  2:2049561/140‑1 (MQ=255)
                                                                      cTTTAACCCTATTACAAACAAAACATCAATCCGGGGCTAATTCAAAAAGTAATTAAGCCCCCTTCCAAAACACTCCTGGCGAGTCACCTTACCATAATTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                    <  2:1570414/140‑1 (MQ=255)
                                                                         tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                                 >  2:2926732/1‑140 (MQ=255)
                                                                         tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                                 >  1:1510951/1‑140 (MQ=255)
                                                                         tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                                 >  1:4173492/1‑140 (MQ=255)
                                                                         tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAGTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTCTGAACAGCTTGCTGCTGAggg                                                                 >  2:2225629/1‑140 (MQ=255)
                                                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                          >  2:3227964/1‑140 (MQ=255)
                                                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                          >  2:466205/1‑140 (MQ=255)
                                                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                          >  2:89563/1‑140 (MQ=255)
                                                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                          >  2:1033501/1‑140 (MQ=255)
                                                                                      aataaaaaaTGCATCCGGGGCTTATTAAAAAAGTAATGACGCTCCCTTCCAAAATACTCGTGGCTAGTCAGTTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                    <  1:400057/137‑1 (MQ=255)
                                                                                           aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                                               >  1:1327939/1‑140 (MQ=255)
                                                                                                 cATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTATCCATCAGTCAAACGACTGTAGAACGTGACTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaagaag                                         >  1:3700559/1‑140 (MQ=255)
                                                                                                   tCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCg                                       >  2:2811727/1‑140 (MQ=255)
                                                                                                    ccggggCGTATTCAAAACGGCATTACCCGCCCTTCCAAAACACTCCTGGCGCGTCAGGTGTCCATAAGACACACGACTGTAGAACGTACCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg                                      <  1:205/138‑1 (MQ=255)
                                                                                                    ccgggcCGTATTCAAAACCTACTGACGCGCACTTCCAAAAACCTCCGGGCGAGTCAGCTTTCCAACAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTTCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg                                      <  1:2362822/138‑1 (MQ=255)
                                                                                                     aGGGGCGTCTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                     >  2:1712136/1‑140 (MQ=255)
                                                                                                     aGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                     >  2:2160956/1‑140 (MQ=255)
                                                                                                     aGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                     >  2:394561/1‑140 (MQ=255)
                                                                                                         gcttATTAAAAACGGAATGAAGCTCCCTCCAAAACCACGCCGGGCGAGTAAGCTTTCAACCCTTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGt                                 <  1:1404748/137‑1 (MQ=255)
                                                                                                         gCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGt                                 >  1:4034243/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGGTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGCTggggtg                              >  2:711503/1‑136 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  1:1624976/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  2:3882975/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  2:984609/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  2:3127825/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  1:2970108/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  1:998199/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  2:1766407/1‑140 (MQ=255)
                                                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATAGCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              >  1:2236895/1‑140 (MQ=255)
                                                                                                            tATTAAAAAAGGAATCAAGCCCCCTTCCAAACGACTCCGGGCGTGTCAGCTTTCCATCAGTCAACCGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                              <  2:2749677/140‑1 (MQ=255)
                                                                                                              ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                            >  1:3383384/1‑140 (MQ=255)
                                                                                                                 aaaaaGTAATGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGTAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                         <  2:1087034/140‑1 (MQ=255)
                                                                                                                     aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                     >  1:1750366/1‑140 (MQ=255)
                                                                                                                               cTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGa           >  2:2701571/1‑140 (MQ=255)
                                                                                                                               cTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGa           >  2:2384157/1‑140 (MQ=255)
                                                                                                                               cTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGa           >  2:1646283/1‑140 (MQ=255)
                                                                                                                                 cccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAtt         >  2:3411827/1‑140 (MQ=255)
                                                                                                                                  ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt        >  2:817542/1‑140 (MQ=255)
                                                                                                                                    ttCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTg      >  1:3537123/1‑140 (MQ=255)
                                                                                                                                      ccAAAACACTCGGGGCGAGTCAGATTTCAATCAGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg    <  1:302012/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTA  >  NZ_CP034943/548973‑549248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: