Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,501 A→C 7.2% E115A (GAA→GCA)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5010AC 7.2% 348.8 / 3.1 149E115A (GAA→GCA) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (48/84);  new base C (7/10);  total (55/94)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

AGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCC  >  NZ_CP034943/607364‑607640
                                                                                                                                         |                                                                                                                                           
cgcgcCGGGAGCCACGGGAGCTTCCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                           <  1:2203443/139‑1 (MQ=255)
  cGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCt                                                                                                                                         <  2:3425726/140‑1 (MQ=255)
     ctggAGCCTCGGGAGCTACCTGGGAATACGGACCAGCAGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                                                      <  2:2446996/138‑1 (MQ=17)
     caggAGCCACGGGAGCTACCGGCGACGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                                                      <  2:4059518/138‑1 (MQ=14)
     cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                                                      <  2:4057578/140‑1 (MQ=255)
     cGGGAGCCACGGGAGCAACCGGCGACTCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGa                                                                                                                                      <  2:1843404/140‑1 (MQ=17)
     cGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCATCCGGACCTACCGGAGCCACAGGAGCTACAGGAGCTACCGGTGACGCGCGACAAGCCTGATCGACAGGCGCGACAGGAGCTACGGGAGCTACCGGGGCAGCTGGa                                                                                                                                      >  1:2735685/1‑140 (MQ=14)
      gggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGCGCGACGGGAGCCACCGGGGCGACCGGGGCGAGCGCGGAAGCAggtc                                                                                                                                     >  2:3109589/1‑138 (MQ=14)
          gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGc                                                                                                                                 >  1:3895575/1‑140 (MQ=255)
           ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                <  1:609267/140‑1 (MQ=255)
           ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                <  1:3642331/140‑1 (MQ=255)
           ccACGGGAGCTACCGGGGAAGCCGGACAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                <  1:3923645/140‑1 (MQ=255)
              cGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGa                                                                                                                             >  2:3185986/1‑140 (MQ=255)
              cGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGa                                                                                                                             >  1:1079260/1‑140 (MQ=255)
                   gCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGAc                                                                                                                        <  1:1507603/140‑1 (MQ=255)
                    cTACCGGAGAACCAGGACCAGACGGACAGCCCGGAGTCAGGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                       <  2:2453901/140‑1 (MQ=14)
                      actggggATGCGGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     <  1:2171726/137‑1 (MQ=17)
                      aCCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     <  1:222074/140‑1 (MQ=255)
                      aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     <  1:1027120/140‑1 (MQ=255)
                      aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGATCCCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGGGCGACggg                                                                                                                     >  1:3500600/1‑140 (MQ=17)
                      aCCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     <  1:3735265/140‑1 (MQ=255)
                          gggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCg                                                                                                                 >  1:867662/1‑140 (MQ=255)
                           cgAAGCCGGACCACCCGGACCAACGGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                                                                                                                <  2:1436406/139‑1 (MQ=255)
                              cgccggaccagccggcccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAg                                                                                                             <  1:1813154/139‑1 (MQ=255)
                                 cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                          >  2:2581600/1‑140 (MQ=255)
                                    agccgccggacccTCCGGAGCGACAGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                                                                                       <  2:3327563/136‑1 (MQ=14)
                                             accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGATCGCCAGGAGCGACCGGAGCGACAGGAGCTAGACGGGAAGCTGGACCAGCTGAAGCGACGGCACCGACAGGAGCGACCGGTGAAg                                                                                              >  2:130789/1‑140 (MQ=17)
                                                  ccGGAGGGACAGGAGCCCCAGGAGCTACCGGCGCCGCCGGACCAGCCGGACCCACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGCAGCAGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                         <  2:2021057/140‑1 (MQ=14)
                                                  ccGGAGCCACTGGAGCGACCGGAGCTCCCGGCGCACCCGGACCAGCCGGACCGCCAGGAGCGACAGGACCGACAGGAGCTCCCGGGGAAGCTGGACCAGCTGGAGGGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                         <  2:201561/140‑1 (MQ=17)
                                                   gggagcgaccggagcgacaGGAGCTAGCGGGGACGCCGGACCAGCCGGACCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                        <  2:3866190/139‑1 (MQ=17)
                                                        cgaccggagcgacaggcgCTCCCGGGGACGCCGGACCACCCGGAGCGACCGGAGCGCCTGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                   <  2:1447595/140‑1 (MQ=17)
                                                        cgaccggagccacaggcgCTCGCGGGGACCCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGcc                                                                                   <  1:2178605/140‑1 (MQ=17)
                                                        cgacaggagcgactggcgCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                   <  1:499466/140‑1 (MQ=255)
                                                        cgacaggagccacaggacCTCCCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGCGCGCCAGGAGCTACCGGCGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                   <  1:3481752/140‑1 (MQ=17)
                                                            aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGCGCGACAGGCGCGCGCGGCGACGCTGGACCACCTGGAGCGACGGGAGCGGCCGGAGCACCCGGGGACGCCGGAGCAGCCgggg                                                                               >  2:3215145/1‑140 (MQ=18)
                                                                   acaggagcTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                        <  1:2459065/140‑1 (MQ=255)
                                                                   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                        <  1:2416061/140‑1 (MQ=255)
                                                                   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTACCGGAGAAGCGGGACCCGCTGGAGCGACGGGCGCGCCAGGACCTACCGGCGCCGCCGGCCCACCCGGGGCCACggg                                                                        >  2:2206777/1‑140 (MQ=18)
                                                                   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                        <  1:2829575/140‑1 (MQ=255)
                                                                   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                        <  1:1346841/140‑1 (MQ=255)
                                                                   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                        <  1:2590323/140‑1 (MQ=255)
                                                                    caggagcACCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGCCTGGAGCTACAGGAGCTCCGGGGGAAGCAGGACCCCCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGa                                                                       <  1:960725/140‑1 (MQ=17)
                                                                        agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACAGGAGCTCCCGGAGACGCAGGACCCTCGGGAGCGACGGGAGCGACAGGAGCTCCCGGGGCAGCCGGACCAGCCGGGGCGACGGGAGcga                                                                   >  2:2978460/1‑138 (MQ=18)
                                                                        agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCAACCGGCGCAGCGGGAGCACCGGGAGCGACGGGCCCCTCAGGAGCAACCGGCGCAGCCGGAGCAGCCGGGGCCACGGGAGccc                                                                   >  2:3273289/1‑139 (MQ=18)
                                                                        agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGCGCCACAGGCGCTCCCGGGGAAGGCGGACCAGCCGGGGCGAGGGGAGCca                                                                   >  2:3294036/1‑140 (MQ=18)
                                                                         gcTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                  <  1:2380552/140‑1 (MQ=255)
                                                                         gcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                  <  1:256005/140‑1 (MQ=255)
                                                                         gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                  >  2:3835028/1‑140 (MQ=255)
                                                                           tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                                <  1:2574942/140‑1 (MQ=255)
                                                                           caCCGGCGAACCCGGACCCGCCGGAGCCACGGGAGCCACCGGAGCGACCGGAGCTACCGGGGCAGCGGGACCAGCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                                <  1:3204632/139‑1 (MQ=17)
                                                                            cccGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                               <  1:2346840/139‑1 (MQ=255)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGGTACCGGAGCTCCAGTCGCGACAGGATCTTCCGGGTAAGCTGGACCAGCTGGAGCTACGGGAGAGACAGGAGCTACCGGGGAAGCCGGACCTGCGGGGGCCACGGGAGCCACAgg                                                               <  1:919400/140‑1 (MQ=18)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACCGGAGCTACCGGGGCAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACggg                                                               >  1:1235756/1‑140 (MQ=18)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGGGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACAGGGGACGCCGGACCAGCCGGGGCCACGGGAGCGACAgg                                                               >  2:617009/1‑140 (MQ=255)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                               <  1:3984741/140‑1 (MQ=255)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                               >  2:272955/1‑140 (MQ=255)
                                                                            aCCGCGGAAGCCGGACCAGCCGGAGCGACACGAGCCACAGGAGCGACATGACCTACCGGGGAAGCTGCAACAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                               <  1:123901/140‑1 (MQ=21)
                                                                                ggctaGCGGGACCAGCCGGAGCGACAGCAGCGACAGGTGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCt                                                           <  2:4234150/136‑1 (MQ=255)
                                                                                 cgcccccGGACCACCCGGACCCCCCGGAGCCACAGGAGCCCCCGGACCCCCCGGCCCCGCGGGACGCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACGAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                          <  2:2658772/135‑1 (MQ=12)
                                                                                 agccacaggACCAGACGGACCCACAGGACCTCAAGGAGCAAAAGGACCTCACGGTCAAGATGGACCCGCGGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                          <  2:3609352/133‑1 (MQ=9)
                                                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGGGCCACGGGAGCCACAGGCGCTAc                                                         >  1:3868191/1‑140 (MQ=255)
                                                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCACGGGCGCCACAGGGGCtgc                                                         >  1:313938/1‑138 (MQ=21)
                                                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGCGCAGCTGGAGCAGCTTGAGCGACGGTAGCGACAGTAGCTACCGGCGAAGCCGGAGCAGCCGGCGCACCGGGAGCAACAGCCGCTAc                                                         >  1:3964796/1‑140 (MQ=18)
                                                                                   aaGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     >  1:2155058/1‑79 (MQ=255)
                                                                                   aaGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                     <  2:2155058/79‑1 (MQ=255)
                                                                                      ccGGCCCAGCCGGAGCCACTGGAGCGACCGGAGCGCCCGGCGCTCCCGGGGCAGCTGGCCCAGCCGGCGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCTGGGGCCACGGGAGCCACAGGGGCTACTggg                                                     <  2:1055229/140‑1 (MQ=18)
                                                                                      ccGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGGGCGACGGGAGCCACAGGGGCTCCgggg                                                     >  1:595089/1‑140 (MQ=21)
                                                                                       cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGAGACGCCGGACCTGCCGGGGCCACCGGAGCGACAGGGGCTACGgggg                                                    >  2:2924665/1‑140 (MQ=18)
                                                                                          aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAg                                                 >  1:2872289/1‑140 (MQ=255)
                                                                                          aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGGGCTACGGGGGACGCCGGACCAGCCGGGGCGCGGGGAGCCAAAGGGGATCCAGGGGCAg                                                 >  1:2835233/1‑140 (MQ=18)
                                                                                               ccGGAGCGACAGGAGCGACAGGGGCCCCCGGAGCAACCGGGGAAGCAGGACCACCTGGAGCCCCCGGAGCGACCGGAGCTACCGGGGAAGCCGGAGCAGCCGGCGCCTCGGGAGCCACAGGGGCTACTGGGGCAGCCGGa                                            >  1:2890505/1‑140 (MQ=18)
                                                                                               ccGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTCCCGGTGAAGCTGGACCAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGa                                            <  1:1999607/140‑1 (MQ=25)
                                                                                               ccGGACCGCCCGGAGCGACTGGAGCGCCTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                            <  1:4112845/140‑1 (MQ=18)
                                                                                                     cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                      <  1:3375197/140‑1 (MQ=255)
                                                                                                       cccggagccaccggagccacaggagcTACAGGCGAAGCTGGACCACCCGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCgg                                    <  2:1795854/137‑1 (MQ=17)
                                                                                                       cccggaccgacaggcgcgccaggagcCACCGGCGCACCCGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                    <  2:3607701/137‑1 (MQ=18)
                                                                                                       ccaggagcgacaggagcgaccggagcTCCCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                    <  1:2476289/139‑1 (MQ=255)
                                                                                                       acaggagcgacaggagcgacaggagcTCCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                    <  2:400747/140‑1 (MQ=255)
                                                                                                       acaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                    <  1:3835028/140‑1 (MQ=255)
                                                                                                         cggagcgacaggagccacagtagcGACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                  <  2:2267394/139‑1 (MQ=255)
                                                                                                         aggagcgacaggagcgacaggagcTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTAGGGGGGAAGCCGGAGCAGCCGGAg                                  >  2:1193131/1‑140 (MQ=255)
                                                                                                         aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                  <  1:874195/140‑1 (MQ=255)
                                                                                                             gcgacaggagcgacagtagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGACCCACAGGGGCTACTGCGCAAGCGGGACCAGCCGGAGCGAc                              >  2:3756403/1‑140 (MQ=21)
                                                                                                             gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCACCGGGGGAATCCGGACCAGCCGGAGCCAc                              >  2:2209997/1‑140 (MQ=21)
                                                                                                                acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCGGGAGCGACCGGAGCGACAGGAGCTACCGGGGCAGCCGGAGCGACCGGGGCCACGGGAGCCACAGGGCCTGCTGGGGAGGCCGGACCACCCGGAGCGACAgg                           >  1:2501731/1‑140 (MQ=21)
                                                                                                                     agccaccggagcTCCCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                      <  1:3555748/140‑1 (MQ=255)
                                                                                                                       cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                   <  2:4161126/93‑1 (MQ=255)
                                                                                                                       cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                   >  1:4161126/1‑93 (MQ=255)
                                                                                                                         acaggagcTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                  <  1:330640/140‑1 (MQ=255)
                                                                                                                         acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCgg                  >  1:3491997/1‑140 (MQ=255)
                                                                                                                           aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCg                                                                                           <  1:2119769/65‑1 (MQ=37)
                                                                                                                               gcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGATACAGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGc            >  1:1793824/1‑140 (MQ=255)
                                                                                                                                cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                   <  1:2735533/68‑1 (MQ=35)
                                                                                                                                cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                           >  1:428802/1‑124 (MQ=255)
                                                                                                                                  aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                   <  2:3916958/50‑1 (MQ=38)
                                                                                                                                  aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                  <  2:3462805/83‑1 (MQ=255)
                                                                                                                                  aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                  >  1:3462805/1‑83 (MQ=255)
                                                                                                                                  aCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCgg         >  2:3692274/1‑140 (MQ=18)
                                                                                                                                         aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc  <  1:1856806/140‑1 (MQ=255)
                                                                                                                                         aaGCTGCCCCACCTGCAGCGACGGGAGCGACACGCGATTCCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc  <  1:571296/140‑1 (MQ=17)
                                                                                                                                         |                                                                                                                                           
AGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCC  >  NZ_CP034943/607364‑607640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: