BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,554	C→G	6.8%	P133A (CCA→GCA)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,554	0	C	G	6.8%	416.9 / 2.7	151	P133A (CCA→GCA)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (69/69); new base G (9/4); total (78/73)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTA  >  NZ_CP034943/607416‑607692

    cgaccggagcgacaggcgCTCCCGGGGACGCCGGACCACCCGGAGCGACCGGAGCGCCTGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                       <  2:1447595/140‑1 (MQ=17)

    cgacaggagcgacaggagCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                       <  1:3299874/140‑1 (MQ=255)

        aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGCGCGACAGGCGCGCGCGGCGACGCTGGACCACCTGGAGCGACGGGAGCGGCCGGAGCACCCGGGGACGCCGGAGCAGCCgggg                                                                                                                                   >  2:3215145/1‑140 (MQ=18)

             cgaccggaccCACCGGCCCAGCCGGACCAGCCGGAGCGCCCGGAGCGACCGGAGCGACAGGACCTACCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                                                              <  2:4043678/140‑1 (MQ=17)

             ccacaggagcTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                                                              <  1:2853115/138‑1 (MQ=255)

               cccggagcTACCGGGGAAGCCGGAACCGCTGTAGCCACGGGAGCGACACGAGCGTTATGAGCTACCGGCGAACCTGGACCAGCTGGAGCGAGGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACggg                                                                                                                            <  1:4185526/137‑1 (MQ=18)

               acaggagcTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                            <  1:2459065/140‑1 (MQ=255)

               acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                            <  1:2416061/140‑1 (MQ=255)

               acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTACCGGAGAAGCGGGACCCGCTGGAGCGACGGGCGCGCCAGGACCTACCGGCGCCGCCGGCCCACCCGGGGCCACggg                                                                                                                            >  2:2206777/1‑140 (MQ=18)

               acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                            <  1:2829575/140‑1 (MQ=255)

                caggagcACCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGCCTGGAGCTACAGGAGCTCCGGGGGAAGCAGGACCCCCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGa                                                                                                                           <  1:960725/140‑1 (MQ=17)

                    agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACAGGAGCTCCCGGAGACGCAGGACCCTCGGGAGCGACGGGAGCGACAGGAGCTCCCGGGGCAGCCGGACCAGCCGGGGCGACGGGAGcga                                                                                                                       >  2:2978460/1‑138 (MQ=18)

                    agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCTACTGGAGAACCCGGGGAAGCGGGACCACCGGGAGCGACCGGAGAGACAGGAGCTCCCGGCGACGCCGGAGCAACCGGTGCCACGGCCGCca                                                                                                                       >  2:3768713/1‑140 (MQ=12)

                    agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCAACCGGCGCAGCGGGAGCACCGGGAGCGACGGGCCCCTCAGGAGCAACCGGCGCAGCCGGAGCAGCCGGGGCCACGGGAGccc                                                                                                                       >  2:3273289/1‑139 (MQ=18)

                    agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGCGCCACAGGCGCTCCCGGGGAAGGCGGACCAGCCGGGGCGAGGGGAGCca                                                                                                                       >  2:3294036/1‑140 (MQ=18)

                     gcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      <  1:256005/140‑1 (MQ=255)

                     gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      >  2:3835028/1‑140 (MQ=255)

                     gcTACCGGCGAAGCCGGACCAGCCGGAGAGAAAGGAGCGTCAGGCTCTTCAGGATCTACCGGGGAAGCTGCACCAGCTGGAGCGAGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      <  1:4120251/140‑1 (MQ=18)

                       caCCGGCGAACCCGGACCCGCCGGAGCCACGGGAGCCACCGGAGCGACCGGAGCTACCGGGGCAGCGGGACCAGCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                                                                                    <  1:3204632/139‑1 (MQ=17)

                        cccGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                   <  1:2346840/139‑1 (MQ=255)

                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACCGGAGCTACCGGGGCAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACggg                                                                                                                   >  1:1235756/1‑140 (MQ=18)

                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                   >  2:272955/1‑140 (MQ=255)

                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                   <  1:3984741/140‑1 (MQ=255)

                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                   >  2:220835/1‑140 (MQ=255)

                        aCCGCGGAAGCCGGACCAGCCGGAGCGACACGAGCCACAGGAGCGACATGACCTACCGGGGAAGCTGCAACAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                   <  1:123901/140‑1 (MQ=21)

                             cgccccaggACCACCCGGAGCCCCAGGACCCAAAGGACCAACAGGAGCAACCGGTCCACCAGGACCCCCGGGAGCGATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACTGGAGCCACAGGGGCTa                                                                                                              <  2:2580291/133‑1 (MQ=2)

                             agccacaggACCAGACGGACCCACAGGACCTCAAGGAGCAAAAGGACCTCACGGTCAAGATGGACCCGCGGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                              <  2:3609352/133‑1 (MQ=9)

                              gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGGGCCACGGGAGCCACAGGCGCTAc                                                                                                             >  1:3868191/1‑140 (MQ=255)

                              gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAATCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGTAAGCCGGAGCAGCCGGGGCCCGGGGAGCAACGGGGTCTAc                                                                                                             >  1:2024425/1‑140 (MQ=18)

                              gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCACGGGCGCCACAGGGGCtgc                                                                                                             >  1:313938/1‑138 (MQ=21)

                              gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGCGCAGCTGGAGCAGCTTGAGCGACGGTAGCGACAGTAGCTACCGGCGAAGCCGGAGCAGCCGGCGCACCGGGAGCAACAGCCGCTAc                                                                                                             >  1:3964796/1‑140 (MQ=18)

                                  ccGGCCCAGCCGGAGCCACTGGAGCGACCGGAGCGCCCGGCGCTCCCGGGGCAGCTGGCCCAGCCGGCGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCTGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                         <  2:1055229/140‑1 (MQ=18)

                                  ccGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGGGCGACGGGAGCCACAGGGGCTCCgggg                                                                                                         >  1:595089/1‑140 (MQ=21)

                                  ccGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                         <  1:2581600/140‑1 (MQ=255)

                                  ccGGACCAGCCGGACCGCCCGGAGGCACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                         <  2:3686302/140‑1 (MQ=18)

                                          gCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGCCCGGAGCTACCGGGGAAGCCGGCCCAGCCGGGGCCACGGGAGCCCCAGGGGCTCCGGGGGAAGCCgg                                                                                                 >  2:2863184/1‑140 (MQ=21)

                                          gCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGCGCGACCGGAGCGCCAGGGGCTACGGGGGAGGCggg                                                                                                 >  2:3333048/1‑140 (MQ=17)

                                           ccGGAGCGACAGGAGCGACAGGGGCCCCCGGAGCAACCGGGGAAGCAGGACCACCTGGAGCCCCCGGAGCGACCGGAGCTACCGGGGAAGCCGGAGCAGCCGGCGCCTCGGGAGCCACAGGGGCTACTGGGGCAGCCGGa                                                                                                >  1:2890505/1‑140 (MQ=18)

                                           ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGa                                                                                                >  1:2157915/1‑140 (MQ=255)

                                           ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCAAGGGATCACCCGGGGCTCGTGGGGAAGCCGGa                                                                                                >  1:3484549/1‑140 (MQ=18)

                                           ccGGAGCGACAGGAGCGACAGGAGACACAGGAGCTACCGGCGAAGCTGGACCAGCTGGCGCGACGGGAGCGACTGGAGAGACCGGGGAAGCCGGACCAGCCGCCGCCCCGGGAGCCACGGGGGCTCCGGGGGAAGCCGGa                                                                                                >  2:3883029/1‑140 (MQ=18)

                                           ccGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTCCCGGTGAAGCTGGACCAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGa                                                                                                <  1:1999607/140‑1 (MQ=25)

                                           ccGGAGCCACAGGACCCACAGGAGCCACAGGAGCAACCGGGCCAGCTGGACAAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                <  1:2730687/140‑1 (MQ=18)

                                           ccGGACCGCCCGGAGCGACTGGAGCGCCTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                <  1:4112845/140‑1 (MQ=18)

                                                 cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                          <  1:3375197/140‑1 (MQ=255)

                                                   cccggagccaccggagccacaggagcTACAGGCGAAGCTGGACCACCCGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCgg                                                                                        <  2:1795854/137‑1 (MQ=17)

                                                   cccggaccgacaggcgcgccaggagcCACCGGCGCACCCGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                        <  2:3607701/137‑1 (MQ=18)

                                                   ccaggagcgacaggagcgaccggagcTCCCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                        <  1:2476289/139‑1 (MQ=255)

                                                   acaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                        <  1:3835028/140‑1 (MQ=255)

                                                     cggagcgacaggagccacagtagcGACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                      <  2:2267394/139‑1 (MQ=255)

                                                     aggagcgacaggagcgacaggagcTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTAGGGGGGAAGCCGGAGCAGCCGGAg                                                                                      >  2:1193131/1‑140 (MQ=255)

                                                     aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                      <  1:874195/140‑1 (MQ=255)

                                                         gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGTAGCGAc                                                                                  >  2:2000415/1‑140 (MQ=255)

                                                            acaggagcgacaggagcTACCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                               <  2:867662/140‑1 (MQ=255)

                                                                 agcgacaggagcTACCGGGGAAGCTGTAACAGCTGGTGCGACGCGAGCGACAGGAGCTACGGGGGAATCCGGGGAAGCCGGAGCCACGGGAGCGACGGGGGCTCCGGGGGAGGCCGGAGCGGGCGGGGCGGCGGGAGCtg                                                                          >  2:3002711/1‑139 (MQ=12)

                                                                 agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGCGCCACTGGAGCCCCCGGGGCGACTGGGGAAGCCGGACCCGCCGGCGCGACTGGAGcga                                                                          >  2:2362241/1‑138 (MQ=18)

                                                                   cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                                                                       <  2:4161126/93‑1 (MQ=255)

                                                                     acaggagcTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                      <  1:330640/140‑1 (MQ=255)

                                                                     acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                      >  1:3491997/1‑140 (MQ=255)

                                                                           gcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGc                                                                >  1:3980128/1‑140 (MQ=255)

                                                                           gcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGATACAGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                >  1:1793824/1‑140 (MQ=255)

                                                                            cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                               >  1:428802/1‑124 (MQ=255)

                                                                            cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                               <  2:428802/124‑1 (MQ=255)

                                                                              aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      >  1:3462805/1‑83 (MQ=255)

                                                                              aCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                             >  2:3692274/1‑140 (MQ=18)

                                                                               ccGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                            <  1:2935726/140‑1 (MQ=255)

                                                                                     ccgCTGCACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGCGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                      <  1:2526673/138‑1 (MQ=255)

                                                                                     aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                      <  1:1856806/140‑1 (MQ=255)

                                                                                     aaGCTGCCCCACCTGCAGCGACGGGAGCGACACGCGATTCCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                      <  1:571296/140‑1 (MQ=17)

                                                                                       gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCgg                                                    >  1:1535554/1‑140 (MQ=255)

                                                                                                gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                           <  1:272955/140‑1 (MQ=255)

                                                                                                    gagccccGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                       <  1:3362600/134‑1 (MQ=14)

                                                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                      >  1:1518327/1‑140 (MQ=255)

                                                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                      >  1:2442150/1‑140 (MQ=255)

                                                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                      <  1:2372407/140‑1 (MQ=255)

                                                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                      >  1:198420/1‑140 (MQ=255)

                                                                                                         aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                  >  1:4098990/1‑140 (MQ=255)

                                                                                                         aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCgg                                  >  1:1380054/1‑140 (MQ=255)

                                                                                                          cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTAGCggg                                 >  2:1099337/1‑140 (MQ=255)

                                                                                                          cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                 >  1:2260807/1‑140 (MQ=255)

                                                                                                          cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACCGGAGCTCCCggg                                 >  1:3618425/1‑140 (MQ=255)

                                                                                                              aGCGACAGGAGCTACCGGCGAAGCCGGAGCCTCCGGCGCCACGGGAGCAACAGGCGCTCCTGGCGAACCGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                             <  1:731881/140‑1 (MQ=17)

                                                                                                                cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGCGAAGCCGGAGCAGCCGGAGCGACAGGAGCGACCGGGCAAGCCGGACCTGCCGGAGCCACAGGAGCGACCGGGGACGcc                           >  1:2034000/1‑140 (MQ=17)

                                                                                                                   cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                        <  2:440146/140‑1 (MQ=255)

                                                                                                                         cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                  >  1:402743/1‑140 (MQ=255)

                                                                                                                         cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGCCCAAcc                  >  1:3460059/1‑140 (MQ=255)

                                                                                                                           aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      >  2:2876488/1‑38 (MQ=255)

                                                                                                                           aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                      <  1:2876488/38‑1 (MQ=255)

                                                                                                                           aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                <  1:220835/140‑1 (MQ=255)

                                                                                                                           aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAAcagc                >  2:2686969/1‑137 (MQ=255)

                                                                                                                           aCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                <  1:2861079/140‑1 (MQ=255)

                                                                                                                           aCCGGCGAAGCGGGAGCTGCCGGTGCCACCGGAGCCACAGGGGCTACAGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                <  2:3886137/140‑1 (MQ=14)

                                                                                                                             cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGCAAGCCGGACCACCCGGAGCGACAGCACGGACCGGGGAAGCCGGACAATCCgggc              >  1:90116/1‑138 (MQ=17)

                                                                                                                                ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                  >  1:2170323/1‑117 (MQ=14)

                                                                                                                                 gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAACCCGGACCAACCGGAGCTAc          >  1:2746548/1‑140 (MQ=255)

                                                                                                                                 gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGAc          >  1:1669828/1‑140 (MQ=255)

                                                                                                                                    gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCgcccgg       >  2:1096040/1‑135 (MQ=17)

                                                                                                                                      cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAg     >  1:639290/1‑140 (MQ=255)

                                                                                                                                      cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCCCAGGAGCTACCGGGGACGCAGGACCAACCGGAGCGAGCGGAg     >  1:2945570/1‑140 (MQ=17)

                                                                                                                                      cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCACCCGGAGGTACAGGAGATACCGGGGAAGCCGGACCATCCGGACCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCgcgggggg     >  1:3614594/1‑133 (MQ=17)

GGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTA  >  NZ_CP034943/607416‑607692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`