Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,628 A→C 33.1% E157D (GAA→GAC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6280AC33.1% 96.4 / 112.6 161E157D (GAA→GACEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (67/40);  new base C (23/31);  total (90/71)
Fisher's exact test for biased strand distribution p-value = 1.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.27e-01

Only 100 of 167 total aligned reads displayed.

GCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACA  >  NZ_CP034943/607491‑607766
                                                                                                                                         |                                                                                                                                          
gcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGc                                                                                                                                          >  1:3980128/1‑140 (MQ=255)
gcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGATACAGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                          >  1:1793824/1‑140 (MQ=255)
    ccggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccactggggctactGgggaagtcgca                                                                                                                                      <  1:94722‑M1/12‑6 (MQ=255)
    ccGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                      <  1:2935726/140‑1 (MQ=255)
     cggggcagccggaccaaccggagcgacgggagccacaggggcgactggggacgccggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGAc                                                                                                                                     >  1:3768713‑M1/128‑140 (MQ=255)
     cggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGAc                                                                                                                                     >  1:3273289‑M1/128‑140 (MQ=255)
        ggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctagtGGGGACGTCGGACCAg                                                                                                                                  <  2:1367811‑M1/16‑1 (MQ=255)
         gaagccggaccagccggagcgacaggagctacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGc                                                                                                                                 <  1:3745902‑M1/17‑1 (MQ=255)
          ccgCTGCACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGCGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                <  1:2526673/138‑1 (MQ=255)
          aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                <  1:1856806/140‑1 (MQ=255)
          aaGCTGCCCCACCTGCAGCGACGGGAGCGACACGCGATTCCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                <  1:571296/140‑1 (MQ=17)
            gccggcccaaccggagccaccgcagccccaggcgctactggcgacgccggaccagccggagccacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCgg                                                                                                                              >  2:859995‑M1/121‑140 (MQ=255)
            gccggaccaaccggagcgacgggagccacaggggctactggggccgtcggaccagccggagcgacaggagctaccggggaagccggacccaccggagcgacgggagccacaggggctacgGGGGACGGCGGACCAGCCgg                                                                                                                              <  2:2609997‑M1/20‑1 (MQ=255)
            gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                              >  1:1535554/1‑140 (MQ=255)
            gCTGGACCAGCTGCCGCGACGGGAGCGACAGGAGCTACCGGGGAAGGCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTGCCGGGGACGCCGGAcaacgcgg                                                                                                                              >  1:3233067/1‑133 (MQ=17)
             cTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGTCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                             <  1:202423/140‑1 (MQ=255)
              tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGCGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGAg                                                                                                                            >  2:1722351/1‑140 (MQ=255)
              cggaccaaccggagcgacgggagccaccggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                            <  2:917434‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggccgtcggaccagccggagcgccaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggcgacgGGGGACGTCGCACCAGCCGGAg                                                                                                                            <  2:1928545‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                            <  1:3829722‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                            <  1:330881‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                            <  2:127386‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                            <  1:1261174‑M1/22‑1 (MQ=255)
                 accaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccaccggggctactGGGGACGTCGGACCAGCCGGAGcgc                                                                                                                         <  1:2713265‑M1/25‑2 (MQ=255)
                     gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGAGCAGCGGGAGCGACAgg                                                                                                                     >  1:1709544/1‑140 (MQ=17)
                     accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                     >  1:2275219‑M1/112‑140 (MQ=255)
                      ccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGa                                                                                                                    >  1:4131207‑M1/111‑140 (MQ=255)
                         gagccccGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                                                 <  1:3362600/134‑1 (MQ=14)
                          cgCGACGGGAGCGCCAGGAGCGACCGGGGATGCCGGAGCCCCCGGGGCCACGGGAGCCACAGGGGCTACCGGGGAAGCCGTCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  1:840817/139‑1 (MQ=17)
                          agcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  2:2970641‑M1/34‑1 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTa                                                                                                                >  1:2992488/1‑140 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  1:2372407/140‑1 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  1:2442150/1‑140 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  1:1518327/1‑140 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  1:198420/1‑140 (MQ=255)
                              acgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                            <  1:3367506‑M1/38‑1 (MQ=255)
                              aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                            >  1:4098990/1‑140 (MQ=255)
                               cgggagccacaggggctcctggcgacggcggaccccccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           >  1:3426350‑M1/102‑140 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGc                                                                                                                                 >  1:2081653‑M1/102‑118 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           <  1:2902705‑M1/39‑1 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAACggg                                                                                                           <  1:3541681‑M1/39‑1 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGCACGTCGGACCAGCCGGAGCGCCAGGAGCTACCggg                                                                                                           <  1:1590195‑M1/39‑1 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTAGCggg                                                                                                           >  2:1099337/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           >  1:2260807/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           >  1:3977424/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACCGGAGCTCCCggg                                                                                                           >  1:3618425/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCGCGCCCGGAGCTCCCggg                                                                                                           >  2:3645988/1‑140 (MQ=17)
                                  gagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGaa                                                                                                        <  1:3854006‑M1/42‑1 (MQ=255)
                                   aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCGGGACCAGCGGGGGCGCCCGGAGCTACCGGGGAAg                                                                                                       >  2:228118/1‑140 (MQ=17)
                                     cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGACGCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGcg                                                                                                     >  2:1124848/1‑139 (MQ=17)
                                        caggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:1690089‑M1/48‑1 (MQ=255)
                                        caggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:2465645‑M1/48‑1 (MQ=255)
                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:440146/140‑1 (MQ=255)
                                         aggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                 >  1:2970641‑M1/92‑140 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  2:877051‑M1/34‑1 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  1:877051‑M1/87‑120 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                            <  2:2605500‑M1/54‑1 (MQ=255)
                                              cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                            >  1:402743/1‑140 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                          <  1:220835/140‑1 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAAcagc                                                                                          >  2:2686969/1‑137 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                          <  1:2861079/140‑1 (MQ=255)
                                                aCCGGCGAAGCGGGAGCTGCCGGTGCCACCGGAGCCACAGGGGCTACAGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                          <  2:3886137/140‑1 (MQ=14)
                                                  cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGCAAGCCGGACCACCCGGAGCGACAGCACGGACCGGGGAAGCCGGACAATCCgggc                                                                                        >  1:90116/1‑138 (MQ=17)
                                                     ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                                                            <  2:2170323/117‑1 (MQ=14)
                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGGAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                                                                                    >  1:2111861/1‑140 (MQ=255)
                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAACCCGGACCAACCGGAGCTAc                                                                                    >  1:2746548/1‑140 (MQ=255)
                                                         gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCgcccgg                                                                                 >  2:1096040/1‑135 (MQ=17)
                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCCCAGGAGCTACCGGGGACGCAGGACCAACCGGAGCGAGCGGAg                                                                               >  1:2945570/1‑140 (MQ=17)
                                                                            cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                              >  2:2952095/1‑140 (MQ=255)
                                                                             gggAGCCACCGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGGCCTGCCCGAGAGACCGAAGCAACGGGGGAGTCCGGACCACCCGGAGCGGCCGGAGCGCGCGGGGAAGCCgggc                                                             >  1:716762/1‑138 (MQ=9)
                                                                             gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCAGAc                                                             >  1:3729140/1‑140 (MQ=14)
                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACTGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                <  1:2234967/140‑1 (MQ=255)
                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                 <  1:2181802/107‑1 (MQ=40)
                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCAACCCGACCAGGCGCAGCGAc                                                >  2:3849351/1‑140 (MQ=14)
                                                                                           cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACAGGAGCGACCGGGGAAGCCGGTCCAGCCGGACCGACa                                               >  2:3058313/1‑140 (MQ=255)
                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCCGAGCTACCGGGGAAGCCCGACCAACCGGAGCGACAGCAGCTACCGCGGCAGCCGGACCCGCCGGAGCGACCgg                                             >  2:2673194/1‑140 (MQ=14)
                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                             >  1:452763/1‑140 (MQ=255)
                                                                                              cTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                            <  2:1518327/140‑1 (MQ=255)
                                                                                                  ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGTCCAACCGGAGCGACCGGAGCTACCGGGGCAGCCGGGGCAGCCGGAGCGACAGgcgcgc                                        >  2:978724/1‑138 (MQ=14)
                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGATCtcc                                       >  1:854100/1‑138 (MQ=255)
                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                       >  2:2122104/1‑140 (MQ=255)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCGCAGCCggagcgaccggagcgacc                                      >  2:1576217/1‑140 (MQ=11)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACAGTAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacc                                      >  1:218521/1‑140 (MQ=11)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                   <  2:2157915/140‑1 (MQ=255)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                   <  1:1963873/140‑1 (MQ=255)
                                                                                                       ccGCACCAGCCGGCCCGACCCGCCCCACCGGGGAATCCGGACCCGCCGGAGCGCCAGGTGTTACCGGGGACCCCGGACCCCCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                   <  2:2890505/140‑1 (MQ=2)
                                                                                                        cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGACCGCCCGGAGCTACCgggg                                  >  2:3977938/1‑140 (MQ=14)
                                                                                                        cGGACCAGCCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGGCCGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGAGTGGAGCGAGCGGGGCCTCCGGACCAGGCGGAGCGGGTGGAGCTCCCgggg                                  >  2:1565452/1‑140 (MQ=14)
                                                                                                           aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                               <  1:1736954/140‑1 (MQ=255)
                                                                                                           aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACACGGAGCGCCCGGAGCTACGGGGGAAGCCGGACCCTCCGTTGCGGCAGGAGCTACCGGGGACGCCGGACCAGCGGGAGCGCGCGGGGCTACGggggtgg                               >  2:1030853/1‑137 (MQ=2)
                                                                                                                          aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                <  1:4067214/140‑1 (MQ=255)
                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGGAGCGCCCGGATCCACCGGCGCAGCCGGAGCACCCGGAGCGCCggg         >  2:555318/1‑140 (MQ=14)
                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAAACGGAGCGACggg         >  2:761841/1‑140 (MQ=14)
                                                                                                                                 aCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACAGGCGAAGCCGGACCAACCGGAGCGACcgg         >  2:2808231/1‑140 (MQ=11)
                                                                                                                                  ccGGGGACGCCGGACCCGCCGGAGCGACAGCAGGTTCCGGGGAAGCCGGACCAACCGGAGCGTCAGGCGCTCCCGGGGACGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa        <  1:2712207/140‑1 (MQ=14)
                                                                                                                                      ggACGCCGGACCAGCCGCAGCGACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCca    <  1:3883029/140‑1 (MQ=255)
                                                                                                                                       gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac   <  1:1124848/138‑1 (MQ=255)
                                                                                                                                       gAAGCCGGACCCGCCGCAGCGACCGGCGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac   <  1:3571658/140‑1 (MQ=14)
                                                                                                                                       ccaGCCGGACCAGCGGGAGCGACAGGCGCTCCCGGCGCAGCCGGACCAACCGCAGGGACTGGCGCTACCGGGGACGCTCGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac   <  2:3460059/138‑1 (MQ=14)
                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca  <  1:2123509/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACA  >  NZ_CP034943/607491‑607766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: