Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,630 C→T 23.4% A158V (GCC→GTC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6300CT23.4% 240.8 / 98.6 167A158V (GCC→GTC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base C (79/48);  new base T (10/28);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 1.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGG  >  NZ_CP034943/607491‑607768
                                                                                                                                           |                                                                                                                                          
    ccggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGa                                                                                                                                        <  1:2566544‑M1/12‑1 (MQ=255)
    ccGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                        <  1:2935726/140‑1 (MQ=255)
     cggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGAc                                                                                                                                       >  1:3273289‑M1/128‑140 (MQ=255)
          caGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                  <  1:3347794/139‑1 (MQ=255)
          aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                  <  1:2270298/140‑1 (MQ=255)
            gccggcccaaccggagccaccgcagccccaggcgctactggcgacgccggaccagccggagccacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCgg                                                                                                                                >  2:859995‑M1/121‑140 (MQ=255)
            gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                >  1:1535554/1‑140 (MQ=255)
            gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGcagc                                                                                                                                >  1:3799250/1‑137 (MQ=255)
              tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGCGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGAg                                                                                                                              >  2:1722351/1‑140 (MQ=255)
              cggaccaaccggagcgacgggagccaccggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  2:917434‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:1261174‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:330881‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:3829722‑M1/22‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  2:127386‑M1/22‑1 (MQ=255)
                 accaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccaccggggctactGGGGACGTCGGACCAGCCGGAGcgc                                                                                                                           <  1:2713265‑M1/25‑2 (MQ=255)
                     gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:1523299/1‑140 (MQ=255)
                     gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:272955/140‑1 (MQ=255)
                      ccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggcgactGGGGACGTCGGACCTGCCGGAGCGAAAGGa                                                                                                                      <  2:2967738‑M1/30‑1 (MQ=255)
                         gAGCGACGGGAGCGACAGGAGCTACCGGGGACCCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                                                   <  1:4107289/140‑1 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTa                                                                                                                  >  1:2992488/1‑140 (MQ=255)
                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  >  1:2442150/1‑140 (MQ=255)
                              acgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGTAGCGACAGGAGCGACCgg                                                                                                              <  1:1825114‑M1/38‑1 (MQ=255)
                              acgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                              <  1:3367506‑M1/38‑1 (MQ=255)
                              acgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                              <  1:3893688‑M1/38‑1 (MQ=255)
                               cgggagccacaggggctcctggcgacggcggaccccccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             >  1:3426350‑M1/102‑140 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGc                                                                                                                                   >  1:2081653‑M1/102‑118 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGc                                                                                                                                   <  2:2081653‑M1/17‑1 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:2902705‑M1/39‑1 (MQ=255)
                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAACggg                                                                                                             <  1:3541681‑M1/39‑1 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             >  1:3977424/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             >  1:4023907/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             >  1:2260807/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACCGGAGCTCCCggg                                                                                                             >  1:3618425/1‑140 (MQ=255)
                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCGCGCCCGGAGCTCCCggg                                                                                                             >  2:3645988/1‑140 (MQ=17)
                                   aGCGACAGGAGCTACCGGCGAAGCCGGAGCCTCCGGCGCCACGGGAGCAACAGGCGCTCCTGGCGAACCGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:731881/140‑1 (MQ=17)
                                     cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGACGCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGcg                                                                                                       >  2:1124848/1‑139 (MQ=17)
                                     cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGCGAAGCCGGAGCAGCCGGAGCGACAGGAGCGACCGGGCAAGCCGGACCTGCCGGAGCCACAGGAGCGACCGGGGACGcc                                                                                                       >  1:2034000/1‑140 (MQ=17)
                                     cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCACCTGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCACCCGGAGCGACAGCAGCGCCCGGGGAAGcc                                                                                                       >  1:2450106/1‑140 (MQ=17)
                                        caggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:1690089‑M1/48‑1 (MQ=255)
                                        caggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:2465618‑M1/48‑1 (MQ=255)
                                        caggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:2465645‑M1/48‑1 (MQ=255)
                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:440146/140‑1 (MQ=255)
                                         aggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   >  1:2970641‑M1/92‑140 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  <  2:877051‑M1/34‑1 (MQ=255)
                                                cccGGGTACGCCGGACCACCCGGCGCCACCGGAGCCACAGGGGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            <  2:2793354/139‑1 (MQ=14)
                                                actggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgactggagccacaggggcaactGGGCAAGTCGCACCAGCCTTAGCGACATGAGGTACAGGCGAAGACGTACCaacccc                                                                                            <  2:1119545‑M1/56‑3 (MQ=255)
                                                aCCGGCGAAGCGGGAGCTGCCGGTGCCACCGGAGCCACAGGGGCTACAGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            <  2:3886137/140‑1 (MQ=14)
                                                  cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAg                                                                                          >  1:1862555/1‑140 (MQ=255)
                                                     ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                                                              >  1:2170323/1‑117 (MQ=14)
                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                      >  1:1669828/1‑140 (MQ=255)
                                                         gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCgcccgg                                                                                   >  2:1096040/1‑135 (MQ=17)
                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAg                                                                                 >  1:639290/1‑140 (MQ=255)
                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCACCCGGAGGTACAGGAGATACCGGGGAAGCCGGACCATCCGGACCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCgcgggggg                                                                                 >  1:3614594/1‑133 (MQ=17)
                                                                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACTGGAGCTACCggg                                                                         >  1:233189/1‑140 (MQ=255)
                                                                   ccGGGGCCACGGGAGCCACAGGGGCTACGGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACCCCGGCCCACCCGGCGCGACCGGAGCGCCCggc                                                                         >  2:2609981/1‑139 (MQ=14)
                                                                             gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCAGAc                                                               >  1:3729140/1‑140 (MQ=14)
                                                                                         ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACACCCGCAGCGACAGGAGCTACCCTGGAAGCCGGACCAGCCGGCGCGa                                                   >  1:3860829/1‑140 (MQ=11)
                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  2:2181802/1‑107 (MQ=40)
                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCAACCCGACCAGGCGCAGCGAc                                                  >  2:3849351/1‑140 (MQ=14)
                                                                                           cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGATCTACCGGGGCATCAGGACCACACGGAGCGACa                                                 >  2:1653260/1‑140 (MQ=11)
                                                                                            tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGTGCAGCCGGACCAGACGGAGCTACAg                                                >  1:4209136/1‑140 (MQ=11)
                                                                                            tACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGCAGCGACAGGAGCAACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCGACGGGCGAAGCCGGCACATCCGGAGCGACAg                                                >  1:952299/1‑140 (MQ=11)
                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCCGAGCTACCGGGGAAGCCCGACCAACCGGAGCGACAGCAGCTACCGCGGCAGCCGGACCCGCCGGAGCGACCgg                                               >  2:2673194/1‑140 (MQ=14)
                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                               >  1:452763/1‑140 (MQ=255)
                                                                                              cTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                              <  2:1518327/140‑1 (MQ=255)
                                                                                                ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                            >  1:859995/1‑140 (MQ=40)
                                                                                                  ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGTCCAACCGGAGCGACCGGAGCTACCGGGGCAGCCGGGGCAGCCGGAGCGACAGgcgcgc                                          >  2:978724/1‑138 (MQ=14)
                                                                                                  ggAACCCGGACCCGCCGGCGCGAGAGGTGCTAGCGGGGTACCCGGACCACCCGGAGCCACAGGAGCTCCCGGCGAATCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                          <  1:4032507/140‑1 (MQ=14)
                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGATCtcc                                         >  1:854100/1‑138 (MQ=255)
                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACGGGCGCAACAGGACCAGACGGAGCGGCTGGatcgca                                         >  1:301037/1‑135 (MQ=11)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                        >  2:26281/1‑140 (MQ=255)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACCCGAGCTACCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                        >  1:293609/1‑140 (MQ=255)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTAcc                                        >  2:2825854/1‑140 (MQ=255)
                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACAGTAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacc                                        >  1:218521/1‑140 (MQ=11)
                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCGACAGCAGCGAACgg                                      >  2:3024251/1‑140 (MQ=11)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                     <  2:2157915/140‑1 (MQ=255)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                     <  1:1963873/140‑1 (MQ=255)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                     <  1:2748471/140‑1 (MQ=255)
                                                                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                     <  1:881466/140‑1 (MQ=255)
                                                                                                       ccGCACCAGCCGGCCCGACCCGCCCCACCGGGGAATCCGGACCCGCCGGAGCGCCAGGTGTTACCGGGGACCCCGGACCCCCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                     <  2:2890505/140‑1 (MQ=2)
                                                                                                        cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGACCGCCCGGAGCTACCgggg                                    >  2:3977938/1‑140 (MQ=14)
                                                                                                        cGGACCAGCCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGGCCGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGAGTGGAGCGAGCGGGGCCTCCGGACCAGGCGGAGCGGGTGGAGCTCCCgggg                                    >  2:1565452/1‑140 (MQ=14)
                                                                                                           aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:2122104/140‑1 (MQ=255)
                                                                                                           aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:1736954/140‑1 (MQ=255)
                                                                                                           aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACACGGAGCGCCCGGAGCTACGGGGGAAGCCGGACCCTCCGTTGCGGCAGGAGCTACCGGGGACGCCGGACCAGCGGGAGCGCGCGGGGCTACGggggtgg                                 >  2:1030853/1‑137 (MQ=2)
                                                                                                                    aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                        >  2:2123509/1‑140 (MQ=255)
                                                                                                                          aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                  <  1:4067214/140‑1 (MQ=255)
                                                                                                                          aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGAGCTGCCGGAGCGACAGCAGATACCGGGGAAGCCGGACCAACCgtcg                  >  2:1389713/1‑137 (MQ=14)
                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGGAGCGCCCGGATCCACCGGCGCAGCCGGAGCACCCGGAGCGCCggg           >  2:555318/1‑140 (MQ=14)
                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAAACGGAGCGACggg           >  2:761841/1‑140 (MQ=14)
                                                                                                                                       gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGTAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:2188697/138‑1 (MQ=255)
                                                                                                                                       gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:1124848/138‑1 (MQ=255)
                                                                                                                                       gcaGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:2209997/138‑1 (MQ=255)
                                                                                                                                       gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGAGCAACCGGAGCGACGGCAGCgac     >  2:3114727/4‑140 (MQ=11)
                                                                                                                                       gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:1431538/137‑1 (MQ=255)
                                                                                                                                       gAATCCGGACGAGCCGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:3756403/140‑1 (MQ=255)
                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:2000415/140‑1 (MQ=255)
                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  1:1722351/140‑1 (MQ=255)
                                                                                                                                       ccaGCCGGACCAGCGGGAGCGACAGGCGCTCCCGGCGCAGCCGGACCAACCGCAGGGACTGGCGCTACCGGGGACGCTCGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac     <  2:3460059/138‑1 (MQ=14)
                                                                                                                                          gCCGGACCACCCGGAGCCGCTGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACTGGACCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg  <  1:3435507/140‑1 (MQ=14)
                                                                                                                                           |                                                                                                                                          
GCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGG  >  NZ_CP034943/607491‑607768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: