Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,674 A→C 9.0% T173P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6740AC 9.0% 257.3 / 4.4 138T173P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (62/51);  new base C (11/5);  total (80/58)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607537‑607813
                                                                                                                                         |                                                                                                                                           
ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                           <  2:2605500‑M1/54‑1 (MQ=255)
cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                           >  1:402743/1‑140 (MQ=255)
cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGCCCAAcc                                                                                                                                           >  1:3460059/1‑140 (MQ=255)
  cccGGGTACGCCGGACCACCCGGCGCCACCGGAGCCACAGGGGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                         <  2:2793354/139‑1 (MQ=14)
  actggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgactggagccacaggggcaactGGGCAAGTCGCACCAGCCTTAGCGACATGAGGTACAGGCGAAGACGTACCaacccc                                                                                                                                         <  2:1119545‑M1/56‑3 (MQ=255)
  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                         <  1:220835/140‑1 (MQ=255)
  aCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                         <  1:2861079/140‑1 (MQ=255)
    cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAg                                                                                                                                       >  1:1862555/1‑140 (MQ=255)
        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGGAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                                                                                                                                   >  1:2111861/1‑140 (MQ=255)
        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                                                                   >  1:1669828/1‑140 (MQ=255)
             cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAg                                                                                                                              >  1:639290/1‑140 (MQ=255)
             cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCCCAGGAGCTACCGGGGACGCAGGACCAACCGGAGCGAGCGGAg                                                                                                                              >  1:2945570/1‑140 (MQ=17)
                     ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACTGGAGCTACCggg                                                                                                                      >  1:233189/1‑140 (MQ=255)
                     ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCCGCGCGCGACGAGCGAGCGGGGAAGCCCCACCAACCTGAGCGACCGCAGCTACCggg                                                                                                                      >  1:3417683/1‑140 (MQ=17)
                     ccGGGGCCACGGGAGCCACAGGGGCTACGGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACCCCGGCCCACCCGGCGCGACCGGAGCGCCCggc                                                                                                                      >  2:2609981/1‑139 (MQ=14)
                              cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                             >  2:2952095/1‑140 (MQ=255)
                              cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAACCGGGCCACCCGGAGCGGCAGGAGGGACCGGGGCAGCCggg                                                                                                             >  2:1059925/1‑139 (MQ=14)
                               gggAGCCACCGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGGCCTGCCCGAGAGACCGAAGCAACGGGGGAGTCCGGACCACCCGGAGCGGCCGGAGCGCGCGGGGAAGCCgggc                                                                                                            >  1:716762/1‑138 (MQ=9)
                                            gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACTGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                               <  1:2234967/140‑1 (MQ=255)
                                            gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                <  1:2181802/107‑1 (MQ=40)
                                            gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCAACCCGACCAGGCGCAGCGAc                                                                                               >  2:3849351/1‑140 (MQ=14)
                                             cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGATCTACCGGGGCATCAGGACCACACGGAGCGACa                                                                                              >  2:1653260/1‑140 (MQ=11)
                                              tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGTGCAGCCGGACCAGACGGAGCTACAg                                                                                             >  1:4209136/1‑140 (MQ=11)
                                              tACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGCAGCGACAGGAGCAACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCGACGGGCGAAGCCGGCACATCCGGAGCGACAg                                                                                             >  1:952299/1‑140 (MQ=11)
                                               aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCCGAGCTACCGGGGAAGCCCGACCAACCGGAGCGACAGCAGCTACCGCGGCAGCCGGACCCGCCGGAGCGACCgg                                                                                            >  2:2673194/1‑140 (MQ=14)
                                               aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                            >  1:452763/1‑140 (MQ=255)
                                                cTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                           <  2:1518327/140‑1 (MQ=255)
                                                  ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                                                                         >  1:859995/1‑140 (MQ=40)
                                                    ggAACCCGGACCCGCCGGCGCGAGAGGTGCTAGCGGGGTACCCGGACCACCCGGAGCCACAGGAGCTCCCGGCGAATCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                       <  1:4032507/140‑1 (MQ=14)
                                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                      <  2:3895575/140‑1 (MQ=255)
                                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                      >  2:2122104/1‑140 (MQ=255)
                                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACGGGCGCAACAGGACCAGACGGAGCGGCTGGatcgca                                                                                      >  1:301037/1‑135 (MQ=11)
                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGCCCAGCCGGAGCTACAGGAGCCCCCGGCGCCGCCCGAGCAGACGGAGAGAcagcagggccc                                                                                     >  2:1239391/1‑135 (MQ=11)
                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCGCAGCCggagcgaccggagcgacc                                                                                     >  2:1576217/1‑140 (MQ=11)
                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCAGGACCATCCGGAGCGACCGGAGCTACCGGGGAGGCGGGAGCAGCCGGAGCGACAGGAgagacc                                                                                     >  2:3546418/1‑135 (MQ=11)
                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                     >  2:26281/1‑140 (MQ=255)
                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACCCGAGCTACCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                     >  1:293609/1‑140 (MQ=255)
                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCGACAGCAGCGAACgg                                                                                   >  2:3024251/1‑140 (MQ=11)
                                                         ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                  <  2:2157915/140‑1 (MQ=255)
                                                         ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                  <  1:2748471/140‑1 (MQ=255)
                                                         ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                  <  1:1963873/140‑1 (MQ=255)
                                                         ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                  <  1:881466/140‑1 (MQ=255)
                                                         ccGCACCAGCCGGCCCGACCCGCCCCACCGGGGAATCCGGACCCGCCGGAGCGCCAGGTGTTACCGGGGACCCCGGACCCCCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                  <  2:2890505/140‑1 (MQ=2)
                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:2122104/140‑1 (MQ=255)
                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:1736954/140‑1 (MQ=255)
                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACACGGAGCGCCCGGAGCTACGGGGGAAGCCGGACCCTCCGTTGCGGCAGGAGCTACCGGGGACGCCGGACCAGCGGGAGCGCGCGGGGCTACGggggtgg                                                                              >  2:1030853/1‑137 (MQ=2)
                                                                      aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                     >  2:2123509/1‑140 (MQ=255)
                                                                           cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGCCCAGCCGGAGCGACAGGACCGACGGGGGCCGCCGGACAAACCggg                                                                >  2:926692/1‑139 (MQ=14)
                                                                            atcaccTACCGGGGACTGCGCACCAGCCGTAGCGACAGGAGATAGCGGGCACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCACCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                               <  1:3710983/135‑1 (MQ=11)
                                                                            aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGAGCTGCCGGAGCGACAGCAGATACCGGGGAAGCCGGACCAACCgtcg                                                               >  2:1389713/1‑137 (MQ=14)
                                                                                  tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACgg                                                         <  2:1523299/140‑1 (MQ=255)
                                                                                   aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGGAGCGCCCGGATCCACCGGCGCAGCCGGAGCACCCGGAGCGCCggg                                                        >  2:555318/1‑140 (MQ=14)
                                                                                   aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAAACGGAGCGACggg                                                        >  2:761841/1‑140 (MQ=14)
                                                                                   aCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACAGGCGAAGCCGGACCAACCGGAGCGACcgg                                                        >  2:2808231/1‑140 (MQ=11)
                                                                                    ccGGGGACGCCGGACCCGCCGGAGCGACAGCAGGTTCCGGGGAAGCCGGACCAACCGGAGCGTCAGGCGCTCCCGGGGACGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                       <  1:2712207/140‑1 (MQ=14)
                                                                                        ggACGCCGGACCAGCCGCAGCGACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCca                                                   <  1:3883029/140‑1 (MQ=255)
                                                                                         gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGTAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  1:2188697/138‑1 (MQ=255)
                                                                                         gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  1:1124848/138‑1 (MQ=255)
                                                                                         gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGAGCAACCGGAGCGACGGCAGCgac                                                  >  2:3114727/4‑140 (MQ=11)
                                                                                         gAATCCGGACGAGCCGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  1:3756403/140‑1 (MQ=255)
                                                                                         gAAGCCGGACCCGCCGCAGCGACCGGCGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  1:3571658/140‑1 (MQ=14)
                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  2:402743/140‑1 (MQ=37)
                                                                                         ccaGCCGGACCAGCGGGAGCGACAGGCGCTCCCGGCGCAGCCGGACCAACCGCAGGGACTGGCGCTACCGGGGACGCTCGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                  <  2:3460059/138‑1 (MQ=14)
                                                                                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                 <  1:2123509/140‑1 (MQ=255)
                                                                                            gCCGGACCACCCGGAGCCGCTGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACTGGACCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                               <  1:3435507/140‑1 (MQ=14)
                                                                                                      ccGGAGCGCCAGGCGCCACCGGCGAACCCGGACCAACCGGAGCCACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                     <  1:503838/140‑1 (MQ=17)
                                                                                                      ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                             >  1:2594040/1‑68 (MQ=35)
                                                                                                      ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                             <  2:2594040/68‑1 (MQ=35)
                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                 >  1:2030450/1‑140 (MQ=255)
                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                 <  1:601781/140‑1 (MQ=255)
                                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                            >  2:579607/1‑140 (MQ=255)
                                                                                                               cAGGAGCGACCGGCGCAGCCGGACCCACCGGAGCCACAGGAGCTCCCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                            <  2:3650567/140‑1 (MQ=18)
                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                  <  1:3160541/53‑1 (MQ=35)
                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                  >  2:3160541/1‑53 (MQ=35)
                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAACCGCAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                           >  1:3591476/1‑140 (MQ=255)
                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                           >  2:3301256/1‑140 (MQ=255)
                                                                                                                       tccGGGGAACCCGGACCAACCGCAGCGCCAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACAACCCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGTCGGACCAGCCgg                    <  1:3333048/139‑1 (MQ=18)
                                                                                                                       cccGGGGCAGCCGGACAAGCCCGAGCGACCGGCGCTGCGGGCGCAGCCGGACCCTCCGGAGCGCCACGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    <  2:3233067/139‑1 (MQ=18)
                                                                                                                       cccGGGCATGCGGGACCCACCGGAGCGAGCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    <  2:3977424/139‑1 (MQ=18)
                                                                                                                       aCCGGGGAAGCTGGTCGGAACGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    <  2:4023907/140‑1 (MQ=18)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCTACGGGAGCCACAGGCGCTACTGGGGACGACGGACCAACCgc                    >  2:4135451/1‑139 (MQ=18)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCgg                    >  2:3900421/1‑140 (MQ=255)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGAccggccgg                    >  1:1118936/1‑140 (MQ=255)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    >  1:4105478/1‑140 (MQ=255)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    >  1:3828329/1‑140 (MQ=255)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    <  1:2863184/140‑1 (MQ=255)
                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGAGACCGGACCGACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                    <  2:3618425/140‑1 (MQ=18)
                                                                                                                         cGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                  <  1:1099337/140‑1 (MQ=255)
                                                                                                                         cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                  <  1:1697522/140‑1 (MQ=255)
                                                                                                                         cGGCGAAGCCGGACCCACCGGAGCGAACGGTGCGACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGATACTGGGGACGTCGGACCAGCCGGAg                  <  1:3645988/140‑1 (MQ=18)
                                                                                                                            ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa               <  1:2673194/140‑1 (MQ=255)
                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                             >  2:3404442/1‑109 (MQ=255)
                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc              >  2:3288444/1‑140 (MQ=255)
                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc              <  1:3288444/140‑1 (MQ=255)
                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc              >  1:4252377/1‑140 (MQ=255)
                                                                                                                             cccaccGGAGCGACCGGAGCCACCGGGGCACCTGCGGCCGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGTGGACGTCGGACCAGCCGGAGCGAc              <  1:3002711/136‑1 (MQ=14)
                                                                                                                               aGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCGGGAGCGACCGGGGCAGCCCGAGCAACCGGAGCCACGGGAGCCCCAGTGGCTCCGGGGGACGTCGGACCAGCCGGAGCGACAg            >  2:3850590/1‑140 (MQ=18)
                                                                                                                                 ccGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCCGCCGGCCCACCCGGAGCGACGGGAGCCCGCGGGGCGACTGGGGAGGTCGGACGAGCCGGAGCGGCCGGa          >  1:3682649/1‑140 (MQ=17)
                                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg         >  1:4086627/1‑140 (MQ=255)
                                                                                                                                     aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCtc      >  1:810284/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607537‑607813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: