BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	132,152	G→A	73.3%	E549K (GAA→AAA)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,152	0	G	A	73.3%	602.2 / 255.3	339	E549K (GAA→AAA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base G (65/25); new base A (144/104); total (209/130)
Fisher's exact test for biased strand distribution p-value = 2.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

GGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAA  >  NZ_CP034943/132014‑132289

        cGGACACGTTTGGATCGAATTCGAACCAAACGAAGCAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGgtg                                                                                                                                  <  1:1226503/140‑1 (MQ=255)

          gACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTa                                                                                                                                <  1:2817921/140‑1 (MQ=255)

          gACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTa                                                                                                                                >  1:3592254/1‑140 (MQ=255)

                tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                                                                                          >  2:1254370/1‑140 (MQ=255)

                tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                                                                                          >  1:1569432/1‑140 (MQ=255)

                tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                                                                                          <  2:1354359/140‑1 (MQ=255)

                  tGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAgg                                                                                                                        >  1:2588584/1‑140 (MQ=255)

                  tGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAgg                                                                                                                        >  1:1519422/1‑140 (MQ=255)

                       cGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTcc                                                                                                                   <  1:1285842/140‑1 (MQ=255)

                        gAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGCTTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTccc                                                                                                                  >  1:3299360/1‑140 (MQ=255)

                         aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGACTACATGCCAGCTGTTCATGCAGGGCTGGAAGATGCCCTTACAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                 >  2:2384129/1‑140 (MQ=255)

                         aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                 >  1:597801/1‑140 (MQ=255)

                                    aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                      <  1:970596/140‑1 (MQ=255)

                                        aagaagGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTaaa                                                                                                  >  1:4144869/1‑140 (MQ=255)

                                              gcgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATc                                                                                                           <  2:600442/125‑1 (MQ=255)

                                               cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAAc                                                                                           >  1:1326113/1‑140 (MQ=255)

                                               cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAAc                                                                                           >  1:739495/1‑140 (MQ=255)

                                                 cttgcttTGAATTTGATAATTGGATTGTCGGTTGGGTAGTTCCACGTGAATACATCCGAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACtt                                                                                         <  2:2249045/140‑1 (MQ=255)

                                                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                    <  2:1956870/140‑1 (MQ=255)

                                                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                    <  2:2818787/140‑1 (MQ=255)

                                                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                    <  2:975765/140‑1 (MQ=255)

                                                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                    <  2:3769165/140‑1 (MQ=255)

                                                          aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGt                                                                                <  1:2485978/140‑1 (MQ=255)

                                                            tttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTc                                                                              <  1:1247761/140‑1 (MQ=255)

                                                             ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCt                                                                             <  2:3684195/140‑1 (MQ=255)

                                                                 caaTGCGATTGGCGGTGGGGTAGGTCCACCTGAATACATCCCAGCTGTTGAAGCAGGTCTGGAAGATGCACATAAAAATGGTGTCCTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACAGTTCGATGGTTCTTAcc                                                                         <  1:3241195/139‑1 (MQ=255)

                                                                 aaaTGCGAGTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTAcc                                                                         <  1:1377608/140‑1 (MQ=255)

                                                                  aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                        <  2:2929048/140‑1 (MQ=255)

                                                                              ggTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTc                                                            <  1:2611331/140‑1 (MQ=255)

                                                                                        ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                  <  1:473838/140‑1 (MQ=255)

                                                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                             >  2:3369231/1‑140 (MQ=255)

                                                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                             >  2:1665521/1‑140 (MQ=255)

                                                                                              gTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTAcc                                                                         <  1:2230646/111‑1 (MQ=255)

                                                                                                 aaTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaa                                         <  2:3299360/140‑1 (MQ=255)

                                                                                                 aaTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaa                                         <  2:74628/140‑1 (MQ=255)

                                                                                                      aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                    <  2:2588584/140‑1 (MQ=255)

                                                                                                      aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                    <  2:2794585/140‑1 (MQ=255)

                                                                                                      aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                    <  2:3578450/140‑1 (MQ=255)

                                                                                                      aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                    >  2:42417/1‑140 (MQ=255)

                                                                                                       tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                   >  2:3003779/1‑140 (MQ=255)

                                                                                                       tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                   >  2:2148804/1‑140 (MQ=255)

                                                                                                       tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                   <  1:891734/140‑1 (MQ=255)

                                                                                                       tCCCAGCTGTTCAAGCAGGTCTGGAAGATGAACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                   <  2:2302571/140‑1 (MQ=255)

                                                                                                        cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                  >  1:2052809/1‑140 (MQ=255)

                                                                                                        cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                  >  2:2853497/1‑140 (MQ=255)

                                                                                                           aGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTgctgctg                               >  1:3526807/1‑139 (MQ=255)

                                                                                                             cTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCt                             >  1:535996/1‑140 (MQ=255)

                                                                                                             cTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCt                             >  2:685160/1‑140 (MQ=255)

                                                                                                             cTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTGAAAGGTGCTGCTTCt                             >  1:3851636/1‑140 (MQ=255)

                                                                                                              tGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                            <  2:903018/140‑1 (MQ=255)

                                                                                                              tGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                            <  2:2644436/140‑1 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  2:1557064/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:791931/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:2262346/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:2706018/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:3092674/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:1805735/1‑140 (MQ=255)

                                                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                          >  1:1465898/1‑140 (MQ=255)

                                                                                                                   aaGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                       <  1:2643978/140‑1 (MQ=255)

                                                                                                                   aaGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGATTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                       <  1:1851437/140‑1 (MQ=255)

                                                                                                                     gCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGATTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                     <  1:4264775/140‑1 (MQ=255)

                                                                                                                        ggTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCaa                                                                                              <  1:2417359/64‑1 (MQ=255)

                                                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                >  2:2101379/1‑140 (MQ=255)

                                                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                >  1:3625018/1‑140 (MQ=255)

                                                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                >  1:3480039/1‑140 (MQ=255)

                                                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                >  1:339069/1‑140 (MQ=255)

                                                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTGACCATGATGTTGACTCAAACGACATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                >  1:2175641/1‑140 (MQ=255)

                                                                                                                               aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                   >  1:3347439/1‑100 (MQ=255)

                                                                                                                               aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                   <  2:1444842/100‑1 (MQ=255)

                                                                                                                               aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACAAAAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa           <  1:1867358/140‑1 (MQ=255)

                                                                                                                               aaGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa           <  1:4003000/140‑1 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAGATGCAGTCa       >  2:3853294/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:3663192/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:192179/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:1147995/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:2837240/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:2964794/1‑140 (MQ=255)

                                                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:1696073/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAATGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:843398/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:4047273/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:951972/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:555563/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:1073894/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:2790364/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:3619352/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:3346726/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:2499573/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:2342811/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:2198249/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  2:1519505/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa       >  1:656065/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAGTTAAAGTTGCTGCTTCTATGGTATTGAAAACTGCAGTCa       >  2:2263750/1‑140 (MQ=255)

                                                                                                                                   tGCACTTAAAAAGGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAATGCAAAACTTTTCGCTGGTTCTTACCATGATGTTGACTCACACGAAGTGGCATTTACAGTTGCTGCGTCTATGGCATTGAAACAGGCAGTCa       >  2:2432790/1‑140 (MQ=255)

                                                                                                                                    gCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg      >  2:4166529/1‑140 (MQ=255)

                                                                                                                                     cACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc     <  1:2286072/140‑1 (MQ=255)

                                                                                                                                     cACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc     <  2:1834183/140‑1 (MQ=255)

                                                                                                                                      aCTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAACAATGCAGTCAGCa    >  2:2705056/1‑140 (MQ=255)

                                                                                                                                      aCTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCa    >  2:1133605/1‑140 (MQ=255)

                                                                                                                                        ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa  >  2:3978583/1‑140 (MQ=255)

                                                                                                                                        ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa  >  1:1017136/1‑140 (MQ=255)

GGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAA  >  NZ_CP034943/132014‑132289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`