Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,700 A→C 8.6% E181D (GAA→GAC) ‡ EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,7000AC 8.6% 293.2 / 10.4 150E181D (GAA→GAC) ‡EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (75/58);  new base C (10/6);  total (86/64)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607567‑607837
                                                                                                                                     |                                                                                                                                         
cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                     >  2:601781/1‑140 (MQ=255)
 gggAGCCACCGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGGCCTGCCCGAGAGACCGAAGCAACGGGGGAGTCCGGACCACCCGGAGCGGCCGGAGCGCGCGGGGAAGCCgggc                                                                                                                                    >  1:716762/1‑138 (MQ=9)
 gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCAGAc                                                                                                                                    >  1:3729140/1‑140 (MQ=14)
             ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACACCCGCAGCGACAGGAGCTACCCTGGAAGCCGGACCAGCCGGCGCGa                                                                                                                        >  1:3860829/1‑140 (MQ=11)
               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGATCTACCGGGGCATCAGGACCACACGGAGCGACa                                                                                                                      >  2:1653260/1‑140 (MQ=11)
               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACAGGAGCGACCGGGGAAGCCGGTCCAGCCGGACCGACa                                                                                                                      >  2:3058313/1‑140 (MQ=255)
                tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGTGCAGCCGGACCAGACGGAGCTACAg                                                                                                                     >  1:4209136/1‑140 (MQ=11)
                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCCGAGCTACCGGGGAAGCCCGACCAACCGGAGCGACAGCAGCTACCGCGGCAGCCGGACCCGCCGGAGCGACCgg                                                                                                                    >  2:2673194/1‑140 (MQ=14)
                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                    >  1:452763/1‑140 (MQ=255)
                    ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                                                                                                 >  1:859995/1‑140 (MQ=40)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                              <  2:3895575/140‑1 (MQ=255)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                              >  2:2122104/1‑140 (MQ=255)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACGGGCGCAACAGGACCAGACGGAGCGGCTGGatcgca                                                                                                              >  1:301037/1‑135 (MQ=11)
                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGCCCAGCCGGAGCTACAGGAGCCCCCGGCGCCGCCCGAGCAGACGGAGAGAcagcagggccc                                                                                                             >  2:1239391/1‑135 (MQ=11)
                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCGCAGCCggagcgaccggagcgacc                                                                                                             >  2:1576217/1‑140 (MQ=11)
                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACCCGAGCTACCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                             >  1:293609/1‑140 (MQ=255)
                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACAGTAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacc                                                                                                             >  1:218521/1‑140 (MQ=11)
                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCGACAGCAGCGAACgg                                                                                                           >  2:3024251/1‑140 (MQ=11)
                           ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                          <  1:1963873/140‑1 (MQ=255)
                           ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                          <  1:881466/140‑1 (MQ=255)
                           ccGCACCAGCCGGCCCGACCCGCCCCACCGGGGAATCCGGACCCGCCGGAGCGCCAGGTGTTACCGGGGACCCCGGACCCCCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                          <  2:2890505/140‑1 (MQ=2)
                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGACCGCCCGGAGCTACCgggg                                                                                                         >  2:3977938/1‑140 (MQ=14)
                            cGGACCAGCCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGGCCGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGAGTGGAGCGAGCGGGGCCTCCGGACCAGGCGGAGCGGGTGGAGCTCCCgggg                                                                                                         >  2:1565452/1‑140 (MQ=14)
                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                      <  1:1736954/140‑1 (MQ=255)
                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACACGGAGCGCCCGGAGCTACGGGGGAAGCCGGACCCTCCGTTGCGGCAGGAGCTACCGGGGACGCCGGACCAGCGGGAGCGCGCGGGGCTACGggggtgg                                                                                                      >  2:1030853/1‑137 (MQ=2)
                                        aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                             >  2:2123509/1‑140 (MQ=255)
                                             cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGCCCAGCCGGAGCGACAGGACCGACGGGGGCCGCCGGACAAACCggg                                                                                        >  2:926692/1‑139 (MQ=14)
                                              aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                       <  1:4067214/140‑1 (MQ=255)
                                              aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGAGCTGCCGGAGCGACAGCAGATACCGGGGAAGCCGGACCAACCgtcg                                                                                       >  2:1389713/1‑137 (MQ=14)
                                                    tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACgg                                                                                 <  2:1523299/140‑1 (MQ=255)
                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGGAGCGCCCGGATCCACCGGCGCAGCCGGAGCACCCGGAGCGCCggg                                                                                >  2:555318/1‑140 (MQ=14)
                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAAACGGAGCGACggg                                                                                >  2:761841/1‑140 (MQ=14)
                                                     aCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACAGGCGAAGCCGGACCAACCGGAGCGACcgg                                                                                >  2:2808231/1‑140 (MQ=11)
                                                      ccGGGGACGCCGGACCCGCCGGAGCGACAGCAGGTTCCGGGGAAGCCGGACCAACCGGAGCGTCAGGCGCTCCCGGGGACGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                                               <  1:2712207/140‑1 (MQ=14)
                                                          ggACGCCGGACCAGCCGCAGCGACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCca                                                                           <  1:3883029/140‑1 (MQ=255)
                                                           gcaGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:2209997/138‑1 (MQ=255)
                                                           gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:1431538/137‑1 (MQ=255)
                                                           gAATCCGGACGAGCCGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:3756403/140‑1 (MQ=255)
                                                           gAAGCCGGACCCGCCGCAGCGACCGGCGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:3571658/140‑1 (MQ=14)
                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  2:402743/140‑1 (MQ=37)
                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:1722351/140‑1 (MQ=255)
                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  1:2123509/140‑1 (MQ=255)
                                                              gCCGGACCACCCGGAGCCGCTGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACTGGACCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  1:3435507/140‑1 (MQ=14)
                                                                        ccGGAGCGCCTGGAGCTCACGGGGAACCCGGACCACCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGATCCCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                             <  1:2119358/140‑1 (MQ=17)
                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                     <  2:2594040/68‑1 (MQ=35)
                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                     >  1:2594040/1‑68 (MQ=35)
                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         <  1:601781/140‑1 (MQ=255)
                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         >  1:2030450/1‑140 (MQ=255)
                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                          >  2:3160541/1‑53 (MQ=35)
                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGGCGGAc                                                   >  1:4130393/1‑140 (MQ=255)
                                                                                         cccGGGGCAGCCGGACAAGCCCGAGCGACCGGCGCTGCGGGCGCAGCCGGACCCTCCGGAGCGCCACGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            <  2:3233067/139‑1 (MQ=18)
                                                                                         cccGGGCATGCGGGACCCACCGGAGCGAGCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            <  2:3977424/139‑1 (MQ=18)
                                                                                         agcGGCGACGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            <  2:3799250/138‑1 (MQ=255)
                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCTACGGGAGCCACAGGCGCTACTGGGGACGACGGACCAACCgc                                            >  2:4135451/1‑139 (MQ=18)
                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGAccggccgg                                            >  1:1118936/1‑140 (MQ=255)
                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            <  1:2863184/140‑1 (MQ=255)
                                                                                         aCCGGGGAAGCCGGACCAACCGGAGAGACCGGACCGACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            <  2:3618425/140‑1 (MQ=18)
                                                                                           cGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                          <  1:1099337/140‑1 (MQ=255)
                                                                                           cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                          <  1:1697522/140‑1 (MQ=255)
                                                                                           cGGCGAAGCCGGACCCACCGGAGCGAACGGTGCGACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGATACTGGGGACGTCGGACCAGCCGGAg                                          <  1:3645988/140‑1 (MQ=18)
                                                                                              ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGACCGACCATCCGGAGCGACCGCAGCCACATGGGCTAATGTGGACGTCGGACCAGCCGTAGCGa                                       >  2:3489235/1‑140 (MQ=17)
                                                                                              ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                       <  1:2673194/140‑1 (MQ=255)
                                                                                              ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGa                                       >  2:2608599/1‑140 (MQ=255)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACAGGGGCTCCTGGGGACGGCGGACCAGCCGGAGCGAc                                      >  2:3341532/1‑140 (MQ=18)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                     <  1:3404442/109‑1 (MQ=255)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                     >  2:3404442/1‑109 (MQ=255)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                      >  2:3288444/1‑140 (MQ=255)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                      <  1:3288444/140‑1 (MQ=255)
                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGAc                                      >  1:608796/1‑140 (MQ=255)
                                                                                               cccaccGGAGCGACCGGAGCCACCGGGGCACCTGCGGCCGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGTGGACGTCGGACCAGCCGGAGCGAc                                      <  1:3002711/136‑1 (MQ=14)
                                                                                                 aGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCGGGAGCGACCGGGGCAGCCCGAGCAACCGGAGCCACGGGAGCCCCAGTGGCTCCGGGGGACGTCGGACCAGCCGGAGCGACAg                                    >  2:3850590/1‑140 (MQ=18)
                                                                                                  cccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   <  1:3185986/139‑1 (MQ=255)
                                                                                                   ccGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCCGCCGGCCCACCCGGAGCGACGGGAGCCCGCGGGGCGACTGGGGAGGTCGGACGAGCCGGAGCGGCCGGa                                  >  1:3682649/1‑140 (MQ=17)
                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                 >  2:405238/1‑140 (MQ=255)
                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                 >  1:4086627/1‑140 (MQ=255)
                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCtc                              >  1:810284/1‑139 (MQ=255)
                                                                                                        ccAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGAGCAGCCGGAGCGACAGGAGCTag                             >  1:492536/1‑139 (MQ=255)
                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGACCGGGGACGCCGGACCCCCCGCAGCGGCGGGAGCCACAGGGGCTACTGGGGCCGGCGGACCAGCCGGTGCGCCAGGGGCGCCCgg                          >  2:3177951/1‑140 (MQ=18)
                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                         >  1:1882223/1‑140 (MQ=255)
                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                     >  2:1697533/1‑140 (MQ=255)
                                                                                                                     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                <  2:198420/140‑1 (MQ=255)
                                                                                                                         cgCTCCCGGGGACGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACGGGTGCGACAGGGGCTTCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa            <  1:1611210/139‑1 (MQ=17)
                                                                                                                         aGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa            <  1:2206777/140‑1 (MQ=255)
                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc           >  2:2956544/1‑140 (MQ=255)
                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:139993/4‑140 (MQ=255)
                                                                                                                             aCCGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:2393733/1‑140 (MQ=255)
                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                              <  2:1821330/86‑1 (MQ=255)
                                                                                                                              ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa       <  1:2608599/140‑1 (MQ=255)
                                                                                                                              ccGGCGAACCCGGACCCGCCGTAGCGACAGCAGCTCCCGGGGAAGCCGGACCACCCGTTGCGACGGGAGCCAAAGGGGATACTGGGGACGTCGGACAAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa       <  1:3489235/140‑1 (MQ=17)
                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg      >  2:3572842/1‑140 (MQ=255)
                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAACCGGAGc     >  2:299233/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGCCCAGCCGCAGCGACACGACCGACCGGGGAAGCCGGCCACCCCGGACCGACGGGAGCCACCGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  2:218521/140‑1 (MQ=17)
                                                                                                                                   gAAGCCGGAGCCGCCGGCGCGACCGGAGCTCCCGGGGAAGCCGGACCCCGCGGAGGGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc  <  2:293609/140‑1 (MQ=14)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGGACCAAACGGAGCGAc  >  2:3663066/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  2:3761860/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  2:3663114/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  2:35414/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGTACAAACCGGAGCGAc  >  2:1013663/1‑140 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  1:1193131/140‑1 (MQ=255)
                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCACGCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGCCAGGAGCTCCGGGGGAAGCCGGACCAACCGCAGCgtc  >  2:2051697/1‑138 (MQ=17)
                                                                                                                                     |                                                                                                                                         
CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607567‑607837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑