BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,783	T→C	6.0%	V209A (GTC→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,783	0	T	C	6.0%	1061.3 / 18.3	385	V209A (GTC→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base T (171/165); new base C (6/17); total (187/198)
Fisher's exact test for biased strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

CAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGC  >  NZ_CP034943/607648‑607921

 aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGCGCATCAGGCCCAACCCGAGCTACGGGAGCCAAATGTGCGACTGGGGCCGTCGGAc                                                                                                                                       >  2:1313458/1‑140 (MQ=17)

        tccGGGGAACCCGGACCAACCGCAGCGCCAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACAACCCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGTCGGACCAGCCgg                                                                                                                                <  1:3333048/139‑1 (MQ=18)

        cccGGGGCAGCCGGACAAGCCCGAGCGACCGGCGCTGCGGGCGCAGCCGGACCCTCCGGAGCGCCACGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  2:3233067/139‑1 (MQ=18)

        aCCGGGGAAGCTGGTCGGAACGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  2:4023907/140‑1 (MQ=18)

        aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCTACGGGAGCCACAGGCGCTACTGGGGACGACGGACCAACCgc                                                                                                                                >  2:4135451/1‑139 (MQ=18)

        aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  1:2863184/140‑1 (MQ=255)

        aCCGGGGAAGCCGGACCAACCGGAGAGACCGGACCGACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  2:3618425/140‑1 (MQ=18)

          cGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:1099337/140‑1 (MQ=255)

          cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:1697522/140‑1 (MQ=255)

             ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                                                                                                           <  1:2673194/140‑1 (MQ=255)

             ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGa                                                                                                                           >  2:2608599/1‑140 (MQ=255)

              gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                                                                                                          <  1:3288444/140‑1 (MQ=255)

                       ccAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGAGCAGCCGGAGCGACAGGAGCTag                                                                                                                 >  1:492536/1‑139 (MQ=255)

                                    cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:198420/140‑1 (MQ=255)

                                        cgCTCCCGGGGACGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACGGGTGCGACAGGGGCTTCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                                <  1:1611210/139‑1 (MQ=17)

                                        aGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                                <  1:2206777/140‑1 (MQ=255)

                                            actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  1:63666/4‑140 (MQ=255)

                                            actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  2:856608/4‑140 (MQ=255)

                                            aCCGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  2:2393733/1‑140 (MQ=255)

                                              cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                          >  2:1825997/1‑140 (MQ=255)

                                               ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAACCGGAGc                                                                                         >  2:299233/1‑140 (MQ=255)

                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGGACCAAACGGAGCGAc                                                                                      >  2:3663066/1‑140 (MQ=255)

                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      <  1:1193131/140‑1 (MQ=255)

                                                   acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCACGAGCGACa                                                                                     >  2:79613/5‑140 (MQ=255)

                                                   acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                     >  2:555327/5‑140 (MQ=255)

                                                   acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                     >  2:3334105/5‑140 (MQ=255)

                                                     gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  1:2719331/3‑140 (MQ=255)

                                                     gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  1:3848909/3‑140 (MQ=255)

                                                     gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  1:1669739/3‑140 (MQ=255)

                                                     gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  1:3390342/3‑140 (MQ=255)

                                                     gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGACAgg                                                                                   >  1:943394/3‑140 (MQ=255)

                                                     gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCgcgggg                                                                                   >  2:3344197/1‑135 (MQ=255)

                                                     gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCCACAGCAGCTACAGGCGAAGACGGAGCAACCGGAGAGACCgg                                                                                   >  2:1421791/1‑140 (MQ=17)

                                                     gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   >  2:1152367/1‑140 (MQ=255)

                                                      tcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                  <  1:1825997/139‑1 (MQ=255)

                                                       cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                                 >  2:2275219/1‑140 (MQ=255)

                                                       cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                 >  1:4017644/1‑140 (MQ=255)

                                                           ccAACCGGCGCCCCAGGACCTCCCGGCGCAGCCGGAGCCACGGGAGCCACGGGAGCCACAGGGGCTGCTGGCGACGCCGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCAc                                                                             <  1:3928432/140‑1 (MQ=2)

                                                            caccgggAGCGACAGGACCCCCCGGGGACCCCGGACCCCCCGGAGCGACGGGAGCACCAGGCGCTACGGGGGACGACGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAca                                                                            <  2:3903872/135‑2 (MQ=14)

                                                             aaccGGCGCCACTGGAGCGACTGGCGCAGCCGGACCACCCGGAGCCACGGGAGCCACAGGCGCTACTGGGGACGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgg                                                                           <  1:4116198/138‑1 (MQ=11)

                                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                          >  1:3017874‑M2/1‑137 (MQ=255)

                                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACagg                                                                          >  1:3114727‑M2/1‑137 (MQ=255)

                                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                          >  1:1389713‑M2/1‑137 (MQ=255)

                                                               ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                         <  1:405238/140‑1 (MQ=255)

                                                                   aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggcta                                                                     >  2:4252377‑M2/1‑132 (MQ=255)

                                                                   aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggcta                                                                     >  2:608796‑M2/1‑132 (MQ=255)

                                                                     cGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcaca                                                                   <  1:3572842/140‑1 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  1:1544576/78‑1 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                                                                                                              >  2:3532557/1‑78 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggg                                                                <  2:1483108‑M2/140‑14 (MQ=255)

                                                                          ggAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACATGGGATTCTGGGGACGTGGGACGAGCATGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                              <  1:2931892/140‑1 (MQ=18)

                                                                             gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCCCGGGAGCcccaggcacagg                                                           >  2:3740335/1‑140 (MQ=255)

                                                                             gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                           <  1:3663066/140‑1 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                     <  1:234408/111‑1 (MQ=255)

                                                                                 ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcgga                                                       <  1:2566544‑M2/140‑23 (MQ=255)

                                                                                  cGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggac                                                      >  1:3768713‑M2/1‑117 (MQ=255)

                                                                                  cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                      >  1:2065881/1‑140 (MQ=255)

                                                                                      gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGGTGCAGCTgg                                                  >  2:3422458/1‑140 (MQ=255)

                                                                                       aaGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                              >  1:2116419/1‑127 (MQ=255)

                                                                                         gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                                                                                                                  >  1:1907025/1‑57 (MQ=255)

                                                                                           cGGACCACCAGGAGCTACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCACCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                             <  1:130789/140‑1 (MQ=21)

                                                                                              aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                          >  1:2490462/1‑140 (MQ=255)

                                                                                              aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGCGCCCCGGGAGCCACAGGCCCAGGAGCAGCTGGGGTCTCCg                                          >  2:2160770/1‑140 (MQ=21)

                                                                                                cAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGaa                                        <  1:1013663/140‑1 (MQ=255)

                                                                                                cAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGaa                                        <  1:35414/140‑1 (MQ=255)

                                                                                                  cccGGCGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGACGGACCAACCCGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  1:1431597/139‑1 (MQ=18)

                                                                                                  aCCGGAGCGTCGGGAGCCACAGGCGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  1:2825854/140‑1 (MQ=255)

                                                                                                  aCCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  1:1239391/140‑1 (MQ=255)

                                                                                                  aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgg                                                                           >  2:3158365/1‑103 (MQ=255)

                                                                                                  aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  2:1882223/140‑1 (MQ=255)

                                                                                                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGAACACCCGGAGCGACAGGAGCCACCGGAGACACAGGACCAGGAGGAGCACGGTTATcaacatgg                                     >  2:2953917/1‑133 (MQ=11)

                                                                                                   ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            <  2:3798197/85‑1 (MQ=255)

                                                                                                   ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacagga                                     >  1:4131207‑M2/1‑100 (MQ=255)

                                                                                                       cgccaCGGGACCCACAGGGGCTACTGGGCAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:3692274/136‑1 (MQ=18)

                                                                                                       agccccGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:1567342/135‑1 (MQ=21)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  2:1728338/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  2:2719331/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGACACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:761841/140‑1 (MQ=255)

                                                                                                            cGGGAGCCACCGGTGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatata                            <  1:1423819/140‑1 (MQ=255)

                                                                                                            cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagc                                                  <  2:2081653‑M2/118‑28 (MQ=255)

                                                                                                               gAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaa                         <  1:3854006‑M2/140‑53 (MQ=255)

                                                                                                                 gCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                      >  2:4124088/1‑93 (MQ=255)

                                                                                                                 gCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGACTACGGGTATATATATaa                       >  1:2463260/1‑140 (MQ=255)

                                                                                                                 gCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATaa                       >  2:490611/1‑140 (MQ=255)

                                                                                                                     caGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccgga                   <  2:1690089‑M2/140‑59 (MQ=255)

                                                                                                                     caGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccgga                   <  2:2465618‑M2/140‑59 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagcta                                 >  1:877051‑M2/1‑76 (MQ=255)

                                                                                                                             aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa           >  2:106500/1‑140 (MQ=255)

                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc        <  1:809083/140‑1 (MQ=255)

                                                                                                                                  ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 >  2:16411/1‑113 (MQ=255)

                                                                                                                                  ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg      >  1:3446708/1‑140 (MQ=255)

                                                                                                                                   gcagTCGGAGCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     <  1:319449/137‑1 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  2:284324/107‑1 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     <  1:555327/140‑1 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     <  1:2967738/140‑1 (MQ=255)

                                                                                                                                    aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                >  1:917078/1‑96 (MQ=255)

                                                                                                                                    aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                <  2:917078/96‑1 (MQ=255)

                                                                                                                                    aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa    >  1:461941/1‑140 (MQ=255)

                                                                                                                                    aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa    >  1:4180615/1‑140 (MQ=255)

                                                                                                                                      gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc  >  2:3834280/1‑140 (MQ=255)

CAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGC  >  NZ_CP034943/607648‑607921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`