BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	767,718	T→G	7.4%	S83R (AGC→CGC)	EO946_RS03930 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	767,718	0	T	G	7.4%	530.6 / 4.9	240	S83R (AGC→CGC)	EO946_RS03930	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (116/85); new base G (16/9); total (146/94)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GGGTCCCTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCC  >  NZ_CP034943/767581‑767856

gggTCCCTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACGAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAc                                                                                                                                          <  2:3190987/140‑1 (MQ=255)

 ggTCCCTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                                                         <  2:280538/140‑1 (MQ=255)

       tGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                                                                   <  2:35651/140‑1 (MQ=255)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGGGGCCCCGCCGCGGGCAGGCACGGCGCCGCGACATATGCCAggcgcg                                                                                                                            >  2:3544789/1‑136 (MQ=14)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccg                                                                                                                            >  2:728812/1‑140 (MQ=255)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                              <  2:2040643/138‑1 (MQ=255)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                              >  1:2040643/1‑138 (MQ=255)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                              <  2:3009711/138‑1 (MQ=255)

              cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCCCCAAGGCCAGGgccg                                                                                                                            >  2:1675560/1‑140 (MQ=255)

                  gccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgc                                                                                                                        <  1:1681168/140‑1 (MQ=255)

                    gagacagaGGCACAGCGCCGCCGCCAATCCCAGCGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                      <  1:2987510/133‑1 (MQ=255)

                    cgAAGCCAGGCACAGCGCCGCCGCCACTCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAAGGCCAGGGccgccgccg                                                                                                                      <  2:791428/140‑1 (MQ=17)

                    cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                      <  2:2586945/140‑1 (MQ=255)

                    cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                      <  1:2197778/140‑1 (MQ=255)

                           aGGCACAGCGCCGCCGCCAATACCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCGGCCAAGGCCCGGGccggcgccgccgcccg                                                                                                               >  2:142959/1‑133 (MQ=14)

                           aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGcccg                                                                                                               >  2:2902801/1‑138 (MQ=255)

                           aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAACCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGGGCCGCCGCCGACGACAGGCAGCCCGCCGCAACCAATGCCAGGGCCGCCGCCGAAGCCAg                                                                                                               >  1:3067931/1‑140 (MQ=18)

                            ggCACAGCGCCGCCGCCAATACCAGGGCCGGTGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAgg                                                                                                              <  2:2079035/140‑1 (MQ=255)

                             gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGCGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGc                                                                                                             >  1:380342/1‑140 (MQ=255)

                             gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGGCGCCGCCAGGCACAGCGCCGCGACCAGTGCCAGCGCCGCCCCCGAACCCAgcc                                                                                                             >  2:3955530/1‑138 (MQ=17)

                                 aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCACTGCCCGGGCCGCCGCCGACGCCAGGCACAg                                                                                                         >  2:2273466/1‑140 (MQ=255)

                                 aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAg                                                                                                         <  1:1388636/140‑1 (MQ=255)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACTGCGCCGCCGCCAATCCCAGGGCCGGAGCACCCGGCGACGCCAGGCCAAGCGCCGAAAACAAACGCAGCGCCGGCGCCAAACACACGcacaccc                                                                                                       >  2:82697/1‑137 (MQ=9)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGCCACACCGCCGCAGCCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAgcc                                                                                                       >  2:2041814/1‑139 (MQ=18)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCACGCACAGCGCCGCCCCCACTCCCCGCCCCGCCGCCGAAGCCACGCACAccg                                                                                                       >  2:4200449/1‑140 (MQ=17)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGACGCCAATGCCAGCGCCGGCGCCGCCGACGAGCCCAGGCAGAGCGCCGCTCCCACTGCCAGGGCCGCCGCCGGCGGCCGGCCCAgcg                                                                                                       >  2:1758895/1‑140 (MQ=14)

                                      cgccgccAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCCATGCCAGGGCCGCCGCCGCAGCCAGGCACAGcgccg                                                                                                    <  1:992940/140‑1 (MQ=255)

                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                    <  1:2517704/140‑1 (MQ=255)

                                          gccacgcccACGGCCGGCGCCGCCGCCGAAGCCCGGCACAGCGCCGCCGCCAATACCAGGGGCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAc                                                                                                <  2:2777657/133‑1 (MQ=18)

                                          gccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAc                                                                                                <  1:112838/140‑1 (MQ=255)

                                           ccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                               <  1:969632/140‑1 (MQ=255)

                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAggg                                                                                     >  1:3088181/1‑140 (MQ=255)

                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATCCCAGCGCCGCCGCCGAAGCCATCCACAGCCCCCAGACCAATGCCAggg                                                                                     >  1:1924890/1‑140 (MQ=18)

                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                >  1:3551800/1‑140 (MQ=255)

                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                >  1:3289089/1‑140 (MQ=255)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAAGGCCACGGCCGCCGCCGAAGCCAGGCCCACCCACGCCACCAAAGCCAGCgccgccgccccc                                                                          >  2:4232285/1‑140 (MQ=17)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCCCCGGCCCCAAAGCCAGCggcgcctccccc                                                                          >  2:2845211/1‑140 (MQ=17)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAAAGCCAGGTCGGGCGACCAAGGCAGGCACAGCCAGGCGACCAACGCCAGGaccgccgccgcc                                                                          >  2:1366539/1‑140 (MQ=17)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGgcccccgccgcc                                                                          >  1:2314089/1‑140 (MQ=255)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGgccgccgccgcc                                                                          >  1:3013151/1‑140 (MQ=255)

                                                                ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCACCAATGCCa                                                                                                                                  >  1:876777/1‑84 (MQ=18)

                                                                    cgcacccAGTCACAGCGCCGCCGCCAAGCCCAGGGCCCGAGCCGCCGCCGAAGCCAGCCACCGCGCCGCTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                      <  1:1182290/135‑1 (MQ=18)

                                                                     gAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                      <  1:693355/91‑1 (MQ=255)

                                                                     gAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                      >  2:693355/1‑91 (MQ=255)

                                                                        gCCAGGCTCAGCGCCGCCGCCAAGACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGCCGCCGCCGCCGAAGc                                                                  <  2:208398/140‑1 (MQ=18)

                                                                           aGGCACAGCGCCGCCGCCAATACCAGGCCCGGAGCCCCCGCCGAAGCCAGGCACAGCGCCGACACCAATCCCCCCGCCCCCGCCCAAAACAGGCACAGCCCCGCAACCAACTCCAGCCCCCCCGCCGCCGCCaaaccaag                                                               >  1:2633701/1‑135 (MQ=9)

                                                                                  gcgcCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgc                                                        >  1:2135084/1‑140 (MQ=255)

                                                                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGACGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  2:1188413/1‑140 (MQ=255)

                                                                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCCAAGCCAGGCACAGCGCCGCAACCAAGGCCAGGGCCCCCGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  2:3597850/1‑140 (MQ=21)

                                                                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGGCGAAGCCAGGCACAGCGACGGGCCCAAGAACAGCGCCGCCGCCGACGACGAACACAGGcgcaccc                                                       >  2:1282180/1‑137 (MQ=17)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACCGCGCCGCTACCAAGCCCAGGGCCGCCGCCGCCGCCGCGGCCCGCCACCGcgccc                                                    >  2:4022674/1‑139 (MQ=18)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACCGCGCCGCGACCAAGGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:3036461/1‑140 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  1:3948570/140‑1 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:1523326/1‑140 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:659593/1‑140 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  1:3459636/140‑1 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:2303627/1‑140 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  1:41231/140‑1 (MQ=255)

                                                                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCCCCGCGACCAAGGCCAGGGCCGCCGCCGCCGCCGAGGCCAGGCACAGcgccg                                                    >  2:1802046/1‑140 (MQ=21)

                                                                                        ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGACAGGCACAGcgccgcc                                                  >  1:2438448/1‑140 (MQ=255)

                                                                                           ccAATACCAGGGGCGGAGCCGCCGCCGAAGCCAGGCACTGCGCCGCTACCAATGCCAGGGCCGCAGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc                                               <  1:4160524/140‑1 (MQ=255)

                                                                                           ccAATACCAGGGCCGGAGCCGCCGCCGATGCCAGCCACAGCGCCGCTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc                                               <  1:2041814/140‑1 (MQ=21)

                                                                                            cacgaCCAGCGCCGTCGCCCCCGCCCAAGCCAGCGACAGCGCCGCTACGCATCCCCCGGCCGCCGCCGAAGCCAGGCACAGCGCCGCCCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCa                                              <  2:3067931/136‑1 (MQ=14)

                                                                                                 cctgggCCTGAGCCGACGCCTTAGCGAGGCACTGCGCCTATACCATGGCCTGGGGCGCCGCCGAAGCCAGGCACAGCGGCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                         <  2:2193653/137‑1 (MQ=17)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCAGGCCCAGCGCCGCACCCAAGGCCAGGGCCCCCGCCGCCGGCGAACCCAGGCACAGCGCCGCCGCCAAGCCCAgcg                                     >  2:1610730/1‑138 (MQ=17)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAAAGCCAgcg                                     >  2:618949/1‑138 (MQ=255)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACGGCCAggg                                     >  2:4123773/1‑140 (MQ=255)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                     >  1:791486/1‑140 (MQ=255)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAAGGCCAggg                                     >  1:1886195/1‑140 (MQ=255)

                                                                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAACCCAGGCACAGCGCCGCCGACAATGCCAggg                                     >  2:3111176/1‑140 (MQ=255)

                                                                                                      gCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTATCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGc                                    >  2:2872346/1‑140 (MQ=255)

                                                                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGTGCCGCCGCCGCCGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGa                                >  1:1565820/1‑140 (MQ=255)

                                                                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                                                         <  1:3343510/35‑1 (MQ=37)

                                                                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                                                         >  2:3343510/1‑35 (MQ=37)

                                                                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGa                                >  1:2229929/1‑140 (MQ=255)

                                                                                                              cgccgcctTAGCGACGCACAGGGGCGCTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGGCGCTACCAATGCCAGGGCGGCGGGCGCCGCCGAAGGCAGGCACCGCGCCGCCGCCAATGCCAGGGCCGGAgccg                            <  2:3986076/140‑1 (MQ=18)

                                                                                                              cgccgccgAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccg                            <  1:2901054/140‑1 (MQ=255)

                                                                                                               gccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgc                           >  2:3502423/1‑140 (MQ=255)

                                                                                                                ccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgcc                          >  1:2890679/1‑140 (MQ=255)

                                                                                                                 cgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccg                         >  2:1065389/1‑140 (MQ=255)

                                                                                                                    cgtacccAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCTCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCACGCACAGCGCCGCCGCCAATTCCAGGGCCGGAGccgccgccg                      <  1:313080/135‑1 (MQ=18)

                                                                                                                    cgtaGCCAGGCACAGCGCCGCTACGAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                      <  2:2650769/137‑1 (MQ=255)

                                                                                                                    cgAAGCCAGGCACAGCTCCGCTAGCATTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                      <  1:3451454/140‑1 (MQ=255)

                                                                                                                    cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                      <  1:3923693/140‑1 (MQ=255)

                                                                                                                    cctcgCCATGCACCGCGCCGCTACCCCGGCCTGGGCCGGCGCCGCAGCCAGGCACAGCGGCGCTACCACTGCCAGCGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                      <  2:2546999/136‑1 (MQ=18)

                                                                                                                         ccAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGcc                 <  1:3770810/140‑1 (MQ=255)

                                                                                                                         ccAGGCACAGCGCCGCGACCAATCCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCGACCAATGCCACGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCGAGGGCCGGAGCCGCCGCCGAAGcc                 <  1:915465/140‑1 (MQ=18)

                                                                                                                                 aGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAg         >  2:379174/1‑140 (MQ=255)

                                                                                                                                 aGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAg         >  2:3210364/1‑140 (MQ=255)

                                                                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg       >  2:2007950/1‑140 (MQ=255)

                                                                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg       >  2:662643/1‑140 (MQ=255)

                                                                                                                                   cgccgcTACCAATGCCAGGCCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAgcg       >  2:3585356/1‑140 (MQ=255)

                                                                                                                                   ccccgcTACCAATGCAATCGCATCCTAAGAAGCCAGGCACAGCGCCGCTACCAATGACATGGCCGCCGCCGCCGCCGAAGCAAGACACAGCCCCCCACCCAATCACAGGGCCTGAGCCCCAGCCTAAGACAGTCACAgcg       >  2:2604829/3‑140 (MQ=9)

                                                                                                                                    gccgcTACCAATGCCAGGGCCGCCGCCGAAGCCATGCACAGCGCCGCTACCAATGCCAGGGCCTCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGGCAGGGCCGGAGCCGCCGCCTAAGCCAGGCACAgcgc      <  1:2604829/140‑1 (MQ=255)

                                                                                                                                    cccgcTCCCAATGACAGGGCCGCCGCCGAAGCCAGGCCCAGAGCCGCTACCACTGCCTGGGACGCCGACGACGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcgc      <  1:1064450/139‑1 (MQ=18)

                                                                                                                                       gcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgc   >  1:2948845/1‑140 (MQ=255)

                                                                                                                                        cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccg                                                                            >  1:1756342/1‑66 (MQ=255)

                                                                                                                                        cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCCCTGCCAGGGCCGGAGCCGCCGCCGACCGCAGGCACAGcgccgcc  >  2:2514342/1‑140 (MQ=18)

                                                                                                                                        cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc  >  1:1793917/1‑140 (MQ=255)

                                                                                                                                        cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc  >  2:38725/1‑140 (MQ=255)

GGGTCCCTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCC  >  NZ_CP034943/767581‑767856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`