Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 767,723 A→C 8.8% I81S (ATT→AGT)  EO946_RS03930 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943767,7230AC 8.8% 520.9 / 12.0 237I81S (ATT→AGT) EO946_RS03930hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (137/67);  new base C (6/21);  total (145/92)
Fisher's exact test for biased strand distribution p-value = 1.07e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

CTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767587‑767861
                                                                                                                                        |                                                                                                                                          
 cgcggcgCACCGCCGACGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                                                                        <  2:3204397/139‑1 (MQ=255)
        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGGGGCCCCGCCGCGGGCAGGCACGGCGCCGCGACATATGCCAggcgcg                                                                                                                                 >  2:3544789/1‑136 (MQ=14)
        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccg                                                                                                                                 >  2:728812/1‑140 (MQ=255)
        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                                   >  1:3009711/1‑138 (MQ=255)
        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                                   <  2:2040927/138‑1 (MQ=255)
        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                                   >  1:2040643/1‑138 (MQ=255)
            ggcgAAGGCAGGCACAGCGCCGGCTCCAATACCACGGCCGGAGCCGCCGCCGAAGCCATGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGTCACAGCCCCGCTACCAATGCAAGGgccgccgc                                                                                                                             <  2:3955569/138‑1 (MQ=17)
            gccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgc                                                                                                                             <  1:1681168/140‑1 (MQ=255)
             ccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAATGCCAGGgccggcgcc                                                                                                                            >  1:3457269/1‑140 (MQ=255)
              cgAAGCCCGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCCGGCACAGCGCCGCCGCCAATACCCGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:1502984/140‑1 (MQ=255)
              cgAAGCCAGGCACAGGGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACCGCGCCGCTACCAAGGCCAGGGccgccgccg                                                                                                                           <  1:1825252/140‑1 (MQ=255)
              cgAAGCCAGGCACAGCGCCGCCGCCACTCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAAGGCCAGGGccgccgccg                                                                                                                           <  2:791428/140‑1 (MQ=17)
              cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:2586945/140‑1 (MQ=255)
                     aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGcccg                                                                                                                    >  2:2902801/1‑138 (MQ=255)
                     aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCACCCAATGCCAGGGCCGCAGCCGCCGCCGAAGCCAGGCACAGCGCCGCACCCAATGCCAGGGCCGCGTCCGAAgaaca                                                                                                                    >  1:2582735/1‑136 (MQ=14)
                      ggCACAGCGCCGCCGCCAATACCAGGGCCGGTGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAgg                                                                                                                   <  2:2079035/140‑1 (MQ=255)
                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGCGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGc                                                                                                                  >  1:380342/1‑140 (MQ=255)
                           aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCACTGCCCGGGCCGCCGCCGACGCCAGGCACAg                                                                                                              >  2:2273466/1‑140 (MQ=255)
                           aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAg                                                                                                              <  1:1388636/140‑1 (MQ=255)
                           aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAg                                                                                                              <  1:976192/140‑1 (MQ=255)
                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACTGCGCCGCCGCCAATCCCAGGGCCGGAGCACCCGGCGACGCCAGGCCAAGCGCCGAAAACAAACGCAGCGCCGGCGCCAAACACACGcacaccc                                                                                                            >  2:82697/1‑137 (MQ=9)
                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAGTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCAGCCGCAGCCAGGCCCAggg                                                                                                            >  2:1259732/1‑138 (MQ=18)
                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCCGGGCCGCCGCCGAAGCCCGGCACAgcc                                                                                                            >  1:337202/1‑139 (MQ=255)
                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCACGCACAGCGCCGCCCCCACTCCCCGCCCCGCCGCCGAAGCCACGCACAccg                                                                                                            >  2:4200449/1‑140 (MQ=17)
                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGACGCCAATGCCAGCGCCGGCGCCGCCGACGAGCCCAGGCAGAGCGCCGCTCCCACTGCCAGGGCCGCCGCCGGCGGCCGGCCCAgcg                                                                                                            >  2:1758895/1‑140 (MQ=14)
                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCCATGCCCGGGCCGCCGCCCAAGCCAGGCCCAGcgccg                                                                                                         >  2:2661040/1‑140 (MQ=21)
                                    gccacgcccACGGCCGGCGCCGCCGCCGAAGCCCGGCACAGCGCCGCCGCCAATACCAGGGGCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAc                                                                                                     <  2:2777657/133‑1 (MQ=18)
                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCGACTGCCAGGGCGGGAGCCGCCGCCGGCGCCAGCCCCCGCGCCGCTCCCAAGGGCAGGGCCGCCGGCGGAGCGAGGCACAGCCCGGCGCCCACTGccggcg                                                                                          >  2:281455/1‑136 (MQ=12)
                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCGCCGCCGACCCCAGGCACAGCGCCCCAACCACGGCCAggg                                                                                          >  2:2361641/1‑140 (MQ=18)
                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                     >  1:2185911/1‑140 (MQ=255)
                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                     >  1:3551800/1‑140 (MQ=255)
                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                     >  1:2981304/1‑140 (MQ=255)
                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                     >  1:3289089/1‑140 (MQ=255)
                                                          ccgccgAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAAGGCCACGGCCGCCGCCGAAGCCAGGCCCACCCACGCCACCAAAGCCAGCgccgccgccccc                                                                               >  2:4232285/1‑140 (MQ=17)
                                                          ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGgcccccgccgcc                                                                               >  1:2314089/1‑140 (MQ=255)
                                                          ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgcc                                                                               >  2:3147862/1‑140 (MQ=255)
                                                          ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCACCAATGCCa                                                                                                                                       >  1:2826998/1‑84 (MQ=18)
                                                          ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCACCAATGCCa                                                                                                                                       >  1:876777/1‑84 (MQ=18)
                                                               gAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:693355/91‑1 (MQ=255)
                                                               gAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           >  2:693355/1‑91 (MQ=255)
                                                                     aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAg                                                                    >  1:113214/1‑140 (MQ=255)
                                                                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCCAAGCCAGGCACAGCGCCGCAACCAAGGCCAGGGCCCCCGCCGCCGCCGAAGCCAGGCACAgcg                                                            >  2:3597850/1‑140 (MQ=21)
                                                                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcc                                                            >  2:1804421/1‑139 (MQ=255)
                                                                                cgcctccAATACCAGGGCCGGAGCCGCCGCCGAATCCAGTCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCTCTACCAATTCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  1:1650163/140‑1 (MQ=21)
                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  1:3948570/140‑1 (MQ=255)
                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         >  2:659593/1‑140 (MQ=255)
                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         >  2:1523326/1‑140 (MQ=255)
                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGCGCCCCCGCCGCCGACGAAGCCAGGCACAGccccg                                                         >  2:1689264/1‑140 (MQ=18)
                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  1:41231/140‑1 (MQ=255)
                                                                                  cggccAATGCCAGGGCCGGAGCCGCCGCCGAACACAGCGACCGCGCCGCTGCCACGGCCCGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGCAGCCGCCGCCGAAGCCAGGCACAGcgccgcc                                                       >  2:1370814/3‑140 (MQ=14)
                                                                                  ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCCGGCACAGCGACGCTACCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccgcc                                                       >  1:760939/1‑140 (MQ=255)
                                                                                  ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGACAGGCACAGcgccgcc                                                       >  1:2438448/1‑140 (MQ=255)
                                                                                     ccAATACCAGGGGCGGAGCCGCCGCCGAAGCCAGGCACTGCGCCGCTACCAATGCCAGGGCCGCAGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc                                                    <  1:4160524/140‑1 (MQ=255)
                                                                                     ccAATACCAGGGCCGGAGCCGCCGCCGATGCCAGCCACAGCGCCGCTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc                                                    <  1:2041814/140‑1 (MQ=21)
                                                                                      cacgaCCAGCGCCGTCGCCCCCGCCCAAGCCAGCGACAGCGCCGCTACGCATCCCCCGGCCGCCGCCGAAGCCAGGCACAGCGCCGCCCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCa                                                   <  2:3067931/136‑1 (MQ=14)
                                                                                      caatcacAGTGCCGGAGCCGCCGCCGAAGCCAGGGACAGCGCCGCTACCATTGCCAGGGCCGCCGCCGAAGCCTGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCa                                                   <  1:2893030/134‑1 (MQ=18)
                                                                                       aaTACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCgccgccgccca                                                  >  2:3011930/1‑138 (MQ=255)
                                                                                           cctgggCCTGAGCCGACGCCTTAGCGAGGCACTGCGCCTATACCATGGCCTGGGGCGCCGCCGAAGCCAGGCACAGCGGCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  2:2193653/137‑1 (MQ=17)
                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAAAGCCAgcg                                          >  2:618949/1‑138 (MQ=255)
                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACGGCCAggg                                          >  2:4123773/1‑140 (MQ=255)
                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  1:1774891/1‑140 (MQ=255)
                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAAGGCCAggg                                          >  1:1886195/1‑140 (MQ=255)
                                                                                                gCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTATCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGc                                         >  2:2872346/1‑140 (MQ=255)
                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGa                                     >  1:2229929/1‑140 (MQ=255)
                                                                                                        cgccgcctTAGCGACGCACAGGGGCGCTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGGCGCTACCAATGCCAGGGCGGCGGGCGCCGCCGAAGGCAGGCACCGCGCCGCCGCCAATGCCAGGGCCGGAgccg                                 <  2:3986076/140‑1 (MQ=18)
                                                                                                         gccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgc                                >  2:3502423/1‑140 (MQ=255)
                                                                                                          ccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGCGCCGGAgccgcc                               >  2:3379910/1‑140 (MQ=255)
                                                                                                           cgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccg                              >  2:1065389/1‑140 (MQ=255)
                                                                                                              cgtacccAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCTCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCACGCACAGCGCCGCCGCCAATTCCAGGGCCGGAGccgccgccg                           <  1:313080/135‑1 (MQ=18)
                                                                                                              cgccgCCAGGCACAGCGCCGCGCCCACTGCCCGGGCCGCCGCCGCAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  2:1649271/136‑1 (MQ=17)
                                                                                                              cgACGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:941270/140‑1 (MQ=255)
                                                                                                              cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:3923693/140‑1 (MQ=255)
                                                                                                              cgAAGCCAGGCACAGCGCCGCTACCAAGCCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  2:2547709/140‑1 (MQ=255)
                                                                                                               gAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                         >  2:3566358/1‑61 (MQ=255)
                                                                                                                 aGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAgg                                                                   <  2:1357298/97‑1 (MQ=255)
                                                                                                                 aGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAgg                                                                   >  1:1357298/1‑97 (MQ=255)
                                                                                                                   ccAGGCACAGCGCCTCTCCCAAGGCCAGGGCCGCCGCCGCAGCCAGGCTCAGCGCCGCTCCCAAGGCGAGGGCCGCCGCCGCCGCCGCAGCCAGGCACAGCGCAGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGcc                      <  2:2043369/140‑1 (MQ=18)
                                                                                                                   ccAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGcc                      <  1:3770810/140‑1 (MQ=255)
                                                                                                                          caGCGCCGCTACCAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCCCTGCCAGCGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGcaca               <  2:1039720/140‑1 (MQ=255)
                                                                                                                           aGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAg              >  2:379174/1‑140 (MQ=255)
                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  2:662643/1‑140 (MQ=255)
                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:3030908/1‑140 (MQ=255)
                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:1931485/1‑140 (MQ=255)
                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  2:2007950/1‑140 (MQ=255)
                                                                                                                             cgccgcTACCAATGCCAGGCCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAgcg            >  2:3585356/1‑140 (MQ=255)
                                                                                                                             ccccgcTACCAATGCAATCGCATCCTAAGAAGCCAGGCACAGCGCCGCTACCAATGACATGGCCGCCGCCGCCGCCGAAGCAAGACACAGCCCCCCACCCAATCACAGGGCCTGAGCCCCAGCCTAAGACAGTCACAgcg            >  2:2604829/3‑140 (MQ=9)
                                                                                                                              gccgcTACCAATGCCAGGGCCGCCGCCGAAGCCATGCACAGCGCCGCTACCAATGCCAGGGCCTCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGGCAGGGCCGGAGCCGCCGCCTAAGCCAGGCACAgcgc           <  1:2604829/140‑1 (MQ=255)
                                                                                                                              cccgcTCCCAATGACAGGGCCGCCGCCGAAGCCAGGCCCAGAGCCGCTACCACTGCCTGGGACGCCGACGACGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcgc           <  1:1064450/139‑1 (MQ=18)
                                                                                                                                 gcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgc        >  1:2948845/1‑140 (MQ=255)
                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc       >  1:3086359/1‑140 (MQ=255)
                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCCCTGCCAGGGCCGGAGCCGCCGCCGACCGCAGGCACAGcgccgcc       >  2:2514342/1‑140 (MQ=18)
                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc       >  2:3040602/1‑140 (MQ=255)
                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc       >  2:38725/1‑140 (MQ=255)
                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc       >  2:2096236/1‑140 (MQ=255)
                                                                                                                                    aCCACTGCCAGGGCCGGCGCCGAAGCCCGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:1342700/140‑1 (MQ=255)
                                                                                                                                    aCCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:2614947/140‑1 (MQ=255)
                                                                                                                                    aCCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:1080632/140‑1 (MQ=255)
                                                                                                                                    aCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:2163842/140‑1 (MQ=255)
                                                                                                                                    aCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:3502423/140‑1 (MQ=255)
                                                                                                                                       aaTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCaa  >  2:4025600/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767587‑767861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: