Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 845,015 T→A 5.4% L316L (CTA→CTT yfkAB ← radical SAM/CxCxxxxC motif protein YfkAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943845,0150TA 5.4% 678.6 / 21.6 199L316L (CTA→CTTyfkABradical SAM/CxCxxxxC motif protein YfkAB
Reads supporting (aligned to +/- strand):  ref base T (98/88);  new base A (5/6);  total (105/94)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

TGATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGT  >  NZ_CP034943/844878‑845154
                                                                                                                                         |                                                                                                                                           
tGATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAg                                                                                                                                           <  1:4008096/140‑1 (MQ=255)
tGATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAg                                                                                                                                           <  1:3240289/140‑1 (MQ=255)
tGATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAg                                                                                                                                           <  1:1728028/140‑1 (MQ=255)
tGATAATAACTGTTTTGGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAg                                                                                                                                           <  1:1548433/140‑1 (MQ=255)
 gATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgc                                                                                                                                          >  2:706752/1‑140 (MQ=255)
 gATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgc                                                                                                                                          >  2:2230337/1‑140 (MQ=255)
 gATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgc                                                                                                                                          >  2:2002285/1‑140 (MQ=255)
 gATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgc                                                                                                                                          >  2:1614132/1‑140 (MQ=255)
 gATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgc                                                                                                                                          >  1:4121625/1‑140 (MQ=255)
  ataataACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgcg                                                                                                                                         >  2:880469/1‑140 (MQ=255)
  ataataACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAgcg                                                                                                                                         >  1:1496185/1‑140 (MQ=255)
       aaCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgt                                                                                                                                    >  1:3272817/1‑140 (MQ=255)
       aaCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgt                                                                                                                                    >  1:1360953/1‑140 (MQ=255)
        aCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCTGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtg                                                                                                                                   >  2:1588680/1‑140 (MQ=255)
         cTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtgt                                                                                                                                  <  1:3489081/140‑1 (MQ=255)
         cTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtgt                                                                                                                                  <  1:277875/140‑1 (MQ=255)
               ttGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGcc                                                                                                                            <  1:858443/140‑1 (MQ=255)
               ttGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGcc                                                                                                                            <  1:928733/140‑1 (MQ=255)
                        aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                   <  2:1014797/140‑1 (MQ=255)
                        aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGAGGCGGTGTATCGCCAAAATCGGt                                                                                                                   <  1:3623300/140‑1 (MQ=255)
                                 ccGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAAt                                                                                                          >  2:3065412/1‑140 (MQ=255)
                                 ccGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAAt                                                                                                          >  2:3505503/1‑140 (MQ=255)
                                   gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                        >  2:2803650/1‑140 (MQ=255)
                                    aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                       >  2:3523984/1‑140 (MQ=255)
                                    aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGACTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                       <  2:1369672/140‑1 (MQ=255)
                                         tCGGACATTCGGACAGTGGGAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                  <  2:2310389/140‑1 (MQ=255)
                                         tCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                  <  2:3759549/140‑1 (MQ=255)
                                          cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTc                                                                                                 >  1:3277774/1‑140 (MQ=255)
                                               aTTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCaaaaa                                                                                            <  2:1978924/140‑1 (MQ=255)
                                                ttCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAAt                                                                                           >  1:169770/1‑140 (MQ=255)
                                                 tCGGACAGTGGCACTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                          >  1:1266288/1‑140 (MQ=255)
                                                 tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                          >  1:2396924/1‑140 (MQ=255)
                                                   ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATGTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                        <  2:2763300/140‑1 (MQ=255)
                                                    gACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTc                                                                                       <  1:2125014/140‑1 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                   >  2:175649/1‑107 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                   <  2:2840739/107‑1 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  2:2493408/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  1:3193093/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  2:1908799/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  2:56441/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  2:523152/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  1:2683604/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGACTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  1:1478035/1‑140 (MQ=255)
                                                         tGGCAATTCAGTTCTTTCGCAAGCTGGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                  >  1:3461761/1‑140 (MQ=255)
                                                              aTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                             <  1:257172/140‑1 (MQ=255)
                                                              aTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                             <  1:3283441/140‑1 (MQ=255)
                                                               ctCAGTTCTTTCGCAAGCTTGGTCTCTCTCCACTTTTCATAGGCGGAAGGGAGGCTGTCCGTCTGAATGTTGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCg                                                                            >  1:1878097/2‑140 (MQ=255)
                                                               ctCAGTTCTTTCGCAAGCTTGGTCTCTCTCCACTTTTCATAGGCGGAAGGGAGGCTGTCCGTCTGAATGTTGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCg                                                                            >  1:1987208/2‑140 (MQ=255)
                                                                   gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                        <  1:1218474/140‑1 (MQ=255)
                                                                   gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                        <  1:417062/140‑1 (MQ=255)
                                                                   gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                        <  1:416424/140‑1 (MQ=255)
                                                                     tCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACg                                                                      <  1:3759504/140‑1 (MQ=255)
                                                                      cTTTCGCAAGCTTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACgg                                                                     <  2:1793109/140‑1 (MQ=255)
                                                                             aaGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAg                                                              <  1:3006846/140‑1 (MQ=255)
                                                                              aGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCAGATTCAGGAGGGAACGTCCGTCAgg                                                             >  2:3360008/1‑140 (MQ=255)
                                                                               gCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAACATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGa                                                            <  2:3273472/140‑1 (MQ=255)
                                                                               gCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGa                                                            <  1:1524364/140‑1 (MQ=255)
                                                                                cTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGAt                                                           <  1:335711/140‑1 (MQ=255)
                                                                                  tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                         <  1:887595/140‑1 (MQ=255)
                                                                                   ggTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCAt                                                        >  2:3909026/1‑140 (MQ=255)
                                                                                       tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                    <  1:1101010/140‑1 (MQ=255)
                                                                                       tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                    <  1:3853355/140‑1 (MQ=255)
                                                                                       tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                    <  1:3496807/140‑1 (MQ=255)
                                                                                         tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                  >  2:2864731/1‑140 (MQ=255)
                                                                                         tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                  <  1:3909564/140‑1 (MQ=255)
                                                                                         tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                  <  1:3110148/140‑1 (MQ=255)
                                                                                         tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                  >  1:2947373/1‑140 (MQ=255)
                                                                                              aTTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                             <  1:3542984/140‑1 (MQ=255)
                                                                                               tttttCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGa                                            >  2:2594787/1‑140 (MQ=255)
                                                                                                     aTAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAAAGGCCGTCAGGATCATTTCTCACCGTGACGtttt                                      >  2:3841160/1‑140 (MQ=255)
                                                                                                            gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCCAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTcc                               >  2:2951421/1‑140 (MQ=255)
                                                                                                            gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGGCAAAAATGTTCACATTCCGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTGTTTCGCTcc                               >  2:2656615/1‑140 (MQ=255)
                                                                                                                    gCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGCCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGcc                       >  1:2052577/1‑140 (MQ=255)
                                                                                                                    gCTGTCCGGCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGGCTCGATAATGTTTCCGTCAAAAATGTTCACATGCTGGCGGGACCGGCCGTCAGGATCCTTTCTCACGGTGACGTTTTTCGCTGCGTGACGcc                       >  2:3214615/1‑140 (MQ=255)
                                                                                                                     cTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCg                      >  1:1598033/1‑140 (MQ=255)
                                                                                                                       gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                    >  1:2065360/1‑140 (MQ=255)
                                                                                                                       gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                    >  1:275787/1‑140 (MQ=255)
                                                                                                                        tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                   >  2:4166967/1‑140 (MQ=255)
                                                                                                                        tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                   >  2:2824403/1‑140 (MQ=255)
                                                                                                                           gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                <  1:534434/140‑1 (MQ=255)
                                                                                                                           gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                <  1:471830/140‑1 (MQ=255)
                                                                                                                           gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                <  1:3948237/140‑1 (MQ=255)
                                                                                                                           ctCTGAATATTGCCTAGCGGCGGTGTAACGACAAAATCGGTCACGAGAATGTTTCCGTCAAAAATGTTCACATACCGCCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                <  1:2843042/139‑1 (MQ=255)
                                                                                                                             cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca              <  1:2479052/140‑1 (MQ=255)
                                                                                                                             cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca              <  1:1366051/140‑1 (MQ=255)
                                                                                                                                     tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa      >  2:1512952/1‑140 (MQ=255)
                                                                                                                                     tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa      >  2:571986/1‑140 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTTTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     <  1:4212481/140‑1 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTGTATCGCCAAACTCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     <  1:1262513/140‑1 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     <  1:4248954/140‑1 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     <  1:2429454/140‑1 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     >  2:2714518/1‑140 (MQ=255)
                                                                                                                                      gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt     <  1:2349715/140‑1 (MQ=255)
                                                                                                                                      gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg      >  1:3817382/1‑138 (MQ=255)
                                                                                                                                      gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg      <  2:3817448/139‑2 (MQ=255)
                                                                                                                                       ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAATCCGGCGAAGCAGCTTTTGATc    >  1:2747904/1‑140 (MQ=255)
                                                                                                                                       ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc    >  1:3684671/1‑140 (MQ=255)
                                                                                                                                       ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc    >  1:627498/1‑140 (MQ=255)
                                                                                                                                         tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt  <  1:1674132/140‑1 (MQ=255)
                                                                                                                                         tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt  <  1:1588680/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGATAATAACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGT  >  NZ_CP034943/844878‑845154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: