Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 845,024 T→G 5.5% T313T (ACA→ACC yfkAB ← radical SAM/CxCxxxxC motif protein YfkAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943845,0240TG 5.5% 564.7 / 15.6 182T313T (ACA→ACCyfkABradical SAM/CxCxxxxC motif protein YfkAB
Reads supporting (aligned to +/- strand):  ref base T (84/88);  new base G (5/5);  total (89/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 198 total aligned reads displayed.

AACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCT  >  NZ_CP034943/844885‑845163
                                                                                                                                           |                                                                                                                                           
aaCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgt                                                                                                                                             >  1:3272817/1‑140 (MQ=255)
 aCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCTGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtg                                                                                                                                            >  2:1588680/1‑140 (MQ=255)
 aCTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtg                                                                                                                                            >  2:1674132/1‑140 (MQ=255)
  cTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtgt                                                                                                                                           <  1:277875/140‑1 (MQ=255)
  cTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGgtgt                                                                                                                                           <  1:3489081/140‑1 (MQ=255)
                 aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                            <  1:971391/140‑1 (MQ=255)
                 aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGAGGCGGTGTATCGCCAAAATCGGt                                                                                                                            <  1:3623300/140‑1 (MQ=255)
                            gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                 >  2:69470/1‑140 (MQ=255)
                            gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                 >  2:2803650/1‑140 (MQ=255)
                             aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                >  2:95702/1‑140 (MQ=255)
                             aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                >  1:1362952/1‑140 (MQ=255)
                             aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGACTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                <  2:1369672/140‑1 (MQ=255)
                                  tCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                           <  2:3202822/140‑1 (MQ=255)
                                  tCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                           <  2:3759549/140‑1 (MQ=255)
                                   cGGACATTCGGACCGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCGGTCTGAAGATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATCATGTTTCCGTc                                                                                                          >  2:3518218/1‑140 (MQ=255)
                                   cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGACGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATACTGTTTCCGTc                                                                                                          >  2:1976896/1‑140 (MQ=255)
                                   cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTc                                                                                                          >  1:3277774/1‑140 (MQ=255)
                                          tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                   >  1:2703960/1‑140 (MQ=255)
                                          tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                   >  1:4189546/1‑140 (MQ=255)
                                          tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                   >  1:2396924/1‑140 (MQ=255)
                                            ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                 <  1:3870816/140‑1 (MQ=255)
                                            ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                 <  1:1237320/140‑1 (MQ=255)
                                            ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATGTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                 <  2:2763300/140‑1 (MQ=255)
                                              aCAGTGGCAATTAATTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAATCGGAAGGGATGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATGGTTAATTATAATGTTTTCGTAAAAAATGttaa                                                                                               <  2:2943025/140‑3 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                            >  2:175649/1‑107 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                            <  2:2840739/107‑1 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:2329611/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:1697560/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:3767112/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:3193093/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  2:3006846/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  2:534434/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCTGGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:3461761/1‑140 (MQ=255)
                                                  tGGCAATTCAGTTCTTTCGCAAGCGTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCCGCGGAATATTGCCGAGCGGCGGTGTATCGCCCAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:2613094/1‑140 (MQ=255)
                                                    gCAATTCAGTTCTTTCGCAAGCTTGGTGTTTCTCCATTGTTCATAAGCGGAAGGGAGGCTGTGCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCCAAATCGGTCACGATTCTGTTTCCGTCAAACCTGTTCACAGTcg                                                                                         >  2:2256467/1‑139 (MQ=255)
                                                    gCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCa                                                                                         >  1:1170007/1‑140 (MQ=255)
                                                      aaTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAgg                                                                                       <  1:1417271/140‑1 (MQ=255)
                                                       aTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                                      <  1:3283441/140‑1 (MQ=255)
                                                       aTTCAGGTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                                      <  1:880469/140‑1 (MQ=255)
                                                        ctCAGTTCTTTCGCAAGCTTGGTCTCTCTCCACTTTTCATAGGCGGAAGGGAGGCTGTCCGTCTGAATGTTGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCg                                                                                     >  1:1878097/2‑140 (MQ=255)
                                                            gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                           >  1:1492846/1‑130 (MQ=255)
                                                            gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                 <  1:417062/140‑1 (MQ=255)
                                                            gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                 <  1:416424/140‑1 (MQ=255)
                                                            gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                 <  1:530839/140‑1 (MQ=255)
                                                            gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                 <  1:534832/140‑1 (MQ=255)
                                                             ttCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAAc                                                                                >  1:172009/1‑140 (MQ=255)
                                                                tttCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGc                                                                             >  1:4256834/1‑140 (MQ=255)
                                                                    gCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTc                                                                         <  1:84042/140‑1 (MQ=255)
                                                                      aaGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAg                                                                       <  1:3006846/140‑1 (MQ=255)
                                                                       aGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCAGATTCAGGAGGGAACGTCCGTCAgg                                                                      >  2:3360008/1‑140 (MQ=255)
                                                                        gCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGa                                                                     <  1:1524364/140‑1 (MQ=255)
                                                                         cTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGAt                                                                    <  1:335711/140‑1 (MQ=255)
                                                                           tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                                  <  1:2039426/140‑1 (MQ=255)
                                                                                tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                             <  1:1101010/140‑1 (MQ=255)
                                                                                tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                             <  1:1643964/140‑1 (MQ=255)
                                                                                tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                             <  1:3496807/140‑1 (MQ=255)
                                                                                  tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                           >  1:2947373/1‑140 (MQ=255)
                                                                                  tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                           <  1:3110148/140‑1 (MQ=255)
                                                                                  tctcCATTTGTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCAAGCGGCGGTGTATCGCCAAACTCGGTCACGATAATGTTTCCGTCAATAATGTTCCCATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                           <  1:4239287/140‑1 (MQ=255)
                                                                                    tcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCAcc                                                         >  1:4095145/1‑140 (MQ=255)
                                                                                       aTTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                                      <  1:3542984/140‑1 (MQ=255)
                                                                                       aTTTGTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTCTCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                                      <  1:3980812/140‑1 (MQ=255)
                                                                                        tttttCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGa                                                     >  2:2594787/1‑140 (MQ=255)
                                                                                              aTAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGtttt                                               <  2:4121625/140‑1 (MQ=255)
                                                                                              aTAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAAAGGCCGTCAGGATCATTTCTCACCGTGACGtttt                                               >  2:3841160/1‑140 (MQ=255)
                                                                                                  gCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGc                                           <  1:2065248/140‑1 (MQ=255)
                                                                                                   cGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCt                                          >  2:4202525/1‑140 (MQ=255)
                                                                                                     gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTcc                                        >  2:3222626/1‑140 (MQ=255)
                                                                                                     gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGGCAAAAATGTTCACATTCCGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTGTTTCGCTcc                                        >  2:2656615/1‑140 (MQ=255)
                                                                                                                gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                             >  1:3196499/1‑140 (MQ=255)
                                                                                                                 tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                            >  2:2824403/1‑140 (MQ=255)
                                                                                                                    gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                         >  1:3937773/1‑140 (MQ=255)
                                                                                                                    gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                         <  1:2077409/140‑1 (MQ=255)
                                                                                                                    ctCTGAATATTGCCTAGCGGCGGTGTAACGACAAAATCGGTCACGAGAATGTTTCCGTCAAAAATGTTCACATACCGCCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                         <  1:2843042/139‑1 (MQ=255)
                                                                                                                      cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca                       <  1:2479052/140‑1 (MQ=255)
                                                                                                                              tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa               >  2:1512952/1‑140 (MQ=255)
                                                                                                                              tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa               >  2:571986/1‑140 (MQ=255)
                                                                                                                              tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg               <  2:1987208/140‑2 (MQ=255)
                                                                                                                              tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg               <  2:2467521/140‑2 (MQ=255)
                                                                                                                              tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg               <  2:1878097/140‑2 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  1:1262861/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  1:2429454/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  2:1076878/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  1:2349715/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  1:904153/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              <  1:1342003/140‑1 (MQ=255)
                                                                                                                               gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt              >  2:2714518/1‑140 (MQ=255)
                                                                                                                               gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg               >  1:3817448/1‑138 (MQ=255)
                                                                                                                               gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg               <  2:3817448/139‑2 (MQ=255)
                                                                                                                                ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc             >  1:1551187/1‑140 (MQ=255)
                                                                                                                                ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc             >  1:3684671/1‑140 (MQ=255)
                                                                                                                                ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc             >  1:2749599/1‑140 (MQ=255)
                                                                                                                                  tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt           <  1:1592168/140‑1 (MQ=255)
                                                                                                                                     cggcggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCtt        >  1:3750407/1‑140 (MQ=255)
                                                                                                                                         ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTTACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc    <  2:3277774/140‑1 (MQ=255)
                                                                                                                                         ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc    <  2:3274587/140‑1 (MQ=255)
                                                                                                                                          gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc   >  2:3600869/1‑140 (MQ=255)
                                                                                                                                          gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc   >  1:229030/1‑140 (MQ=255)
                                                                                                                                          gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc   >  1:1955675/1‑140 (MQ=255)
                                                                                                                                           tgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCAGTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCt  <  2:179309/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AACTGTTTTTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCT  >  NZ_CP034943/844885‑845163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: