Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 845,033 A→G 5.1% F310F (TTT→TTC yfkAB ← radical SAM/CxCxxxxC motif protein YfkAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943845,0330AG 5.1% 658.8 / 15.9 195F310F (TTT→TTCyfkABradical SAM/CxCxxxxC motif protein YfkAB
Reads supporting (aligned to +/- strand):  ref base A (89/94);  new base G (5/5);  total (96/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGCA  >  NZ_CP034943/844897‑845170
                                                                                                                                        |                                                                                                                                         
cGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCaaaa                                                                                                                                        <  1:3294114/140‑1 (MQ=255)
     aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  2:1014797/140‑1 (MQ=255)
     aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  1:3411101/140‑1 (MQ=255)
     aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGAGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  1:3623300/140‑1 (MQ=255)
     aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTATCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  1:3476012/140‑1 (MQ=255)
              ccGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAAt                                                                                                                          >  2:3505503/1‑140 (MQ=255)
                gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                        >  2:69470/1‑140 (MQ=255)
                gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                        >  2:2803650/1‑140 (MQ=255)
                 aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                       >  2:95702/1‑140 (MQ=255)
                 aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                       >  2:3523984/1‑140 (MQ=255)
                 aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGACTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                       <  2:1369672/140‑1 (MQ=255)
                      tCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                                  <  2:3202822/140‑1 (MQ=255)
                       cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGACGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATACTGTTTCCGTc                                                                                                                 >  2:1976896/1‑140 (MQ=255)
                       cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTc                                                                                                                 >  1:3274587/1‑140 (MQ=255)
                             ttCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAAt                                                                                                           >  1:169770/1‑140 (MQ=255)
                              tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                          >  1:2396924/1‑140 (MQ=255)
                                ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                        <  1:1237320/140‑1 (MQ=255)
                                  aCAGTGGCAATTAATTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAATCGGAAGGGATGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATGGTTAATTATAATGTTTTCGTAAAAAATGttaa                                                                                                      <  2:2943025/140‑3 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  2:2840739/107‑1 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   <  1:175649/107‑1 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                   >  2:175649/1‑107 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  1:2683604/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  1:3767112/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  1:3193093/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  1:2329611/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  2:1545028/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  2:2493408/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  2:2744913/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  2:3006846/1‑140 (MQ=255)
                                      tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  2:534434/1‑140 (MQ=255)
                                        gCAATTCAGTTCTTTCGCAAGCTTGGTGTTTCTCCATTGTTCATAAGCGGAAGGGAGGCTGTGCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCCAAATCGGTCACGATTCTGTTTCCGTCAAACCTGTTCACAGTcg                                                                                                >  2:2256467/1‑139 (MQ=255)
                                        gCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCa                                                                                                >  1:1170007/1‑140 (MQ=255)
                                          aaTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAgg                                                                                              <  1:1417271/140‑1 (MQ=255)
                                           aTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                                             <  1:257172/140‑1 (MQ=255)
                                           aTTCAGGTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                                             <  1:880469/140‑1 (MQ=255)
                                            ctCAGTTCTTTCGCAAGCTTGGTCTCTCTCCACTTTTCATAGGCGGAAGGGAGGCTGTCCGTCTGAATGTTGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCg                                                                                            >  1:1878097/2‑140 (MQ=255)
                                                gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  <  2:1492846/130‑1 (MQ=255)
                                                gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                  >  1:1492846/1‑130 (MQ=255)
                                                gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                        <  1:416692/140‑1 (MQ=255)
                                                          aaGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAg                                                                              <  1:3006846/140‑1 (MQ=255)
                                                            gCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGa                                                                            <  1:1524364/140‑1 (MQ=255)
                                                               tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                                         <  1:2039426/140‑1 (MQ=255)
                                                                ggTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCAt                                                                        >  2:3909026/1‑140 (MQ=255)
                                                                    tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                    <  1:1643964/140‑1 (MQ=255)
                                                                    tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                    <  1:1101010/140‑1 (MQ=255)
                                                                    tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                    <  1:4136491/140‑1 (MQ=255)
                                                                    gttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                    <  1:3496837/139‑1 (MQ=255)
                                                                      tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                                  <  1:3110148/140‑1 (MQ=255)
                                                                      tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                                  <  1:3909564/140‑1 (MQ=255)
                                                                      tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                                  >  1:2947373/1‑140 (MQ=255)
                                                                           aTTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                                             <  1:3542984/140‑1 (MQ=255)
                                                                           aTTTGTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTCTCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                                             <  1:3980812/140‑1 (MQ=255)
                                                                                      gCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGc                                                  <  1:2065248/140‑1 (MQ=255)
                                                                                       cGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCt                                                 >  2:4202525/1‑140 (MQ=255)
                                                                                         gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTcc                                               >  2:3222626/1‑140 (MQ=255)
                                                                                         gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGGCAAAAATGTTCACATTCCGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTGTTTCGCTcc                                               >  2:2656615/1‑140 (MQ=255)
                                                                                                    gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                                    >  1:3196499/1‑140 (MQ=255)
                                                                                                    gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                                    >  1:275787/1‑140 (MQ=255)
                                                                                                     tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                                   >  2:4166967/1‑140 (MQ=255)
                                                                                                     tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                                   >  2:2824403/1‑140 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGTTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  2:3827727/140‑1 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  2:2595997/140‑1 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  1:3948237/140‑1 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  1:2077409/140‑1 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  1:534434/140‑1 (MQ=255)
                                                                                                        gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  1:471830/140‑1 (MQ=255)
                                                                                                        ctCTGAATATTGCCTAGCGGCGGTGTAACGACAAAATCGGTCACGAGAATGTTTCCGTCAAAAATGTTCACATACCGCCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                                <  1:2843042/139‑1 (MQ=255)
                                                                                                          cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca                              <  1:2479052/140‑1 (MQ=255)
                                                                                                          cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca                              <  1:1366051/140‑1 (MQ=255)
                                                                                                                  tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa                      >  2:571986/1‑140 (MQ=255)
                                                                                                                  tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                      <  2:1878097/140‑2 (MQ=255)
                                                                                                                  tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                      <  2:1987208/140‑2 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTTTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:4212481/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:2429454/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:904153/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:4248954/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:1342003/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:2349715/140‑1 (MQ=255)
                                                                                                                   gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                     <  1:1262861/140‑1 (MQ=255)
                                                                                                                   gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                      <  2:3817382/139‑2 (MQ=255)
                                                                                                                   gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                      >  1:3817448/1‑138 (MQ=255)
                                                                                                                    ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATTTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                    >  1:3282087/1‑140 (MQ=255)
                                                                                                                    ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAATCCGGCGAAGCAGCTTTTGATc                    >  1:2747904/1‑140 (MQ=255)
                                                                                                                    ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                    >  1:1551187/1‑140 (MQ=255)
                                                                                                                    ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                    >  1:627498/1‑140 (MQ=255)
                                                                                                                    ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                    >  1:3684671/1‑140 (MQ=255)
                                                                                                                      tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt                  <  1:1592168/140‑1 (MQ=255)
                                                                                                                       agacgcgtTGTATCGCCAAAATCTTTAAAGATAATGTTTCCTTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTc                 >  2:1155910/5‑140 (MQ=255)
                                                                                                                         cggcggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCtt               >  1:3750407/1‑140 (MQ=255)
                                                                                                                             ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc           <  2:3274587/140‑1 (MQ=255)
                                                                                                                             ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc           <  1:4171109/140‑1 (MQ=255)
                                                                                                                              gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc          >  1:229030/1‑140 (MQ=255)
                                                                                                                              gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc          >  1:1955675/1‑140 (MQ=255)
                                                                                                                               tgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCt         <  2:3346771/140‑1 (MQ=255)
                                                                                                                               tgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCAGTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCt         <  2:179309/140‑1 (MQ=255)
                                                                                                                                   tCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTCAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACt     >  1:1695376/1‑140 (MQ=255)
                                                                                                                                     gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc   <  2:2703960/140‑1 (MQ=255)
                                                                                                                                     gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc   <  1:386236/140‑1 (MQ=255)
                                                                                                                                     gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc   <  2:2396924/140‑1 (MQ=255)
                                                                                                                                      ccAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGCa  >  2:3716846/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGCA  >  NZ_CP034943/844897‑845170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: