BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	132,185	G→A	20.0%	D560N (GAC→AAC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,185	0	G	A	20.0%	885.9 / 205.0	385	D560N (GAC→AAC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base G (172/135); new base A (51/26); total (223/162)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 386 total aligned reads displayed.

ACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132046‑132322

        aagaagGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTaaa                                                                                                                                   >  1:2332652/1‑140 (MQ=255)

                 cttgcttTGAATTTGATAATTGGATTGTCGGTTGGGTAGTTCCACGTGAATACATCCGAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACtt                                                                                                                          <  2:2249045/140‑1 (MQ=255)

                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:975765/140‑1 (MQ=255)

                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                                                     <  1:3523125/140‑1 (MQ=255)

                      tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:1445881/140‑1 (MQ=255)

                            tttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTc                                                                                                               <  1:1254370/140‑1 (MQ=255)

                               gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAGTAAAGCAAAACTTTTCGATGGTTCTTa                                                                                                            >  1:4226172/1‑140 (MQ=255)

                                  aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:595480/140‑1 (MQ=255)

                                  aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:2417473/140‑1 (MQ=255)

                                   aTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTAATGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCat                                                                                                        <  2:2319810/140‑1 (MQ=255)

                                    tGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGATGTTCACGCAGGTCTGGAAGATGCACTTAAGATTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAATCTTTTCGATGGTTCGTagccgg                                                                                                       >  2:3257573/1‑135 (MQ=255)

                                              ggTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTc                                                                                             <  1:2611331/140‑1 (MQ=255)

                                               gTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCa                                                                                            <  2:2349635/140‑1 (MQ=255)

                                                      aGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGaaa                                                                                     >  1:739127/1‑140 (MQ=255)

                                                        ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   <  1:473838/140‑1 (MQ=255)

                                                        gtCCACGTGAATACATCCCCTCTGTTCCAGCAGGTCTGGAAGATGCACTTAACAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAACCTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   <  1:2476618/139‑1 (MQ=255)

                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:1665521/1‑140 (MQ=255)

                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1813265/1‑140 (MQ=255)

                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:732145/1‑140 (MQ=255)

                                                             cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:1311765/1‑140 (MQ=255)

                                                                 aaTACATCCCCGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaa                                                                          <  2:2543190/140‑1 (MQ=255)

                                                                     cATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtg                                                                      <  2:288785/140‑1 (MQ=255)

                                                                      aTCCCAGCTGTTCAAGCAGGTCTGGAATATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  2:2940752/140‑1 (MQ=255)

                                                                       tCCCAGCTGTTCAAGCAGGTCTGGAATATGCAGTTGAAAATGGTGTAGTGTCAGGATTCACGCTTATCAACATTAAAGCAAAAATTTTCGATTGTTCTTACCATTATCTTGACACAAAAGAAATGGCATTTAAAGTttct                                                                    >  2:419045/1‑140 (MQ=255)

                                                                        cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  1:2052809/1‑140 (MQ=255)

                                                                         ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctgc                                                                  <  1:3445416/140‑1 (MQ=255)

                                                                           aGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  1:997895/136‑1 (MQ=255)

                                                                             cTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCt                                                              >  2:685160/1‑140 (MQ=255)

                                                                              tGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                                                             <  1:36144/140‑1 (MQ=255)

                                                                              tGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                                                             <  2:2192701/140‑1 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:1557064/1‑140 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:1865963/1‑140 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:1504904/1‑140 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:3092674/1‑140 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:791931/1‑140 (MQ=255)

                                                                                ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:2706018/1‑140 (MQ=255)

                                                                                  cAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaa                                                                           <  1:2251534/122‑1 (MQ=255)

                                                                                   aaGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                        <  1:922688/140‑1 (MQ=255)

                                                                                   aaGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                        <  1:2643978/140‑1 (MQ=255)

                                                                                   aaGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGATTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                        <  2:3335794/140‑1 (MQ=255)

                                                                                          tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:4059385/1‑140 (MQ=255)

                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:2702152/1‑140 (MQ=255)

                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:3684220/1‑140 (MQ=255)

                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:338984/1‑140 (MQ=255)

                                                                                          tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTGACCATGATGTTGACTCAAACGACATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:2175641/1‑140 (MQ=255)

                                                                                                   ttcACTTAAAAAAGGTGTACTGTAATACATACAGCTTATCGACATTAAAGCAAAAATTTTCGATGTTTCTTAAAATGATGTTGACTCAAACGAAATGTCATTTAAAGTTGCTGCTTCTATTGCATTGAAAAATGAAGTCa                                        >  2:2130717/3‑140 (MQ=255)

                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:192179/1‑140 (MQ=255)

                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:133754/1‑140 (MQ=255)

                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:3303065/1‑140 (MQ=255)

                                                                                                   tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTGAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2825133/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTcc                                        >  2:3536995/1‑139 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2803816/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2832901/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:3872643/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:878954/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1941992/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1073894/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1519505/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1159598/1‑140 (MQ=255)

                                                                                                   tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAGTTAAAGTTGCTGCTTCTATGGTATTGAAAACTGCAGTCa                                        >  2:2263750/1‑140 (MQ=255)

                                                                                                     cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  1:2857184/140‑1 (MQ=255)

                                                                                                     cACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:1834183/140‑1 (MQ=255)

                                                                                                        ttaaaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                   >  2:1627918/4‑140 (MQ=255)

                                                                                                        ttaaaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCACCaaa                                   >  1:3065093/4‑140 (MQ=255)

                                                                                                        ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                   >  1:1017136/1‑140 (MQ=255)

                                                                                                        ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                   >  1:1656080/1‑140 (MQ=255)

                                                                                                         taaaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAAt                                  >  1:123865/3‑140 (MQ=255)

                                                                                                          aaaaaTTGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:2090426/139‑1 (MQ=255)

                                                                                                          aaaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 >  2:3670053/2‑140 (MQ=255)

                                                                                                          aaaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:685160/139‑1 (MQ=255)

                                                                                                             aaTGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTaa                              <  1:2130717/140‑1 (MQ=255)

                                                                                                                  tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  2:2736440/1‑140 (MQ=255)

                                                                                                                  tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  1:2946808/1‑140 (MQ=255)

                                                                                                                   gtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  1:1766302/140‑1 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         >  2:3938328/1‑58 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  1:3938328/58‑1 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:3832614/1‑140 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:944352/1‑140 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       <  1:3872643/140‑1 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:558777/1‑140 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:115672/1‑140 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  1:143460/1‑140 (MQ=255)

                                                                                                                    tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:2601644/1‑140 (MQ=255)

                                                                                                                        ggCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGttcttc                   <  1:2748690/140‑1 (MQ=255)

                                                                                                                            ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  1:3637306/1‑140 (MQ=255)

                                                                                                                              cTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             <  2:336761/140‑1 (MQ=255)

                                                                                                                              cTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             <  2:3594726/140‑1 (MQ=255)

                                                                                                                                tCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  1:3775077/140‑1 (MQ=255)

                                                                                                                                tCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  1:559699/140‑1 (MQ=255)

                                                                                                                                tCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  2:3303065/140‑1 (MQ=255)

                                                                                                                                 cccGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCaa          >  2:2477000/1‑140 (MQ=255)

                                                                                                                                 cccGCTTATCGACATTAAAGCAAAAATTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCGTCTATGGCATAGAAAAATGCAGTCCGCAAATGTAACCCAGTTCTACTGGAAGCaa          >  2:4246696/1‑140 (MQ=255)

                                                                                                                                     cTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  2:4144869/140‑1 (MQ=255)

                                                                                                                                       tATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 >  1:2542315/1‑111 (MQ=255)

                                                                                                                                       tATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 >  1:2273796/1‑111 (MQ=255)

                                                                                                                                       tATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa    >  2:4009504/1‑140 (MQ=255)

                                                                                                                                        aTCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  1:3214090/140‑1 (MQ=255)

                                                                                                                                        aTCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  2:8217/140‑1 (MQ=255)

                                                                                                                                        aTCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  1:581259/140‑1 (MQ=255)

                                                                                                                                         tCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaaa  >  2:3066226/1‑140 (MQ=255)

ACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132046‑132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`