Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,112,969 C→T 10.2% L1022F (CTT→TTT)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,112,9690CT10.2% 871.3 / 65.6 297L1022F (CTT→TTT) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (158/104);  new base T (16/14);  total (178/119)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 297 total aligned reads displayed.

TACGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCTTG  >  NZ_CP034943/1112831‑1113106
                                                                                                                                          |                                                                                                                                         
  cGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTg                                                                                                                                        <  2:772790/140‑1 (MQ=255)
  cGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTg                                                                                                                                        >  1:1314100/1‑140 (MQ=255)
  cGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTg                                                                                                                                        >  1:2132613/1‑140 (MQ=255)
     aCTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGACTGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGc                                                                                                                                     >  1:100944/1‑140 (MQ=255)
     aCTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGc                                                                                                                                     >  2:2345741/1‑140 (MQ=255)
     aCTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGc                                                                                                                                     >  2:653902/1‑140 (MQ=255)
     aCTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGc                                                                                                                                     >  1:2110326/1‑140 (MQ=255)
     aCTGGCGCTGCAAATGCTGACGTACCTTGATGTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGc                                                                                                                                     >  1:1540323/1‑140 (MQ=255)
       tGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCtt                                                                                                                                   >  2:1185077/1‑140 (MQ=255)
       tGGCGCTGCAAATGCAGACGTACCTTGATTAATCGATTACTCATTCAGCCGACTGGCTCGGAATCAGGGCGACGCCGGCAGGAGTGCTGTATATCCAAATACATGACCCGATCATTCAATCTAAACTCACCCATGGGCta                                                                                                                                   >  2:4162906/1‑139 (MQ=255)
         gcgcTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGa                                                                                                                                 >  1:2997675/1‑140 (MQ=255)
              gCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGATTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACgaga                                                                                                                            <  1:590980/140‑1 (MQ=255)
              gCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACgaga                                                                                                                            <  2:4196769/140‑1 (MQ=255)
                   tGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGaa                                                                                                                       >  1:4217369/1‑140 (MQ=255)
                    gCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATATCACGCTAGGGCTTGACGAGATTGAAc                                                                                                                      >  2:1099054/1‑140 (MQ=255)
                       gACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAgg                                                                                                                   <  1:2345741/140‑1 (MQ=255)
                         cGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGgag                                                                                                                 >  1:3890718/1‑140 (MQ=255)
                         cGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGgag                                                                                                                 >  2:1252554/1‑140 (MQ=255)
                               ttcaTTTATAGATTACTCATTCAGCCGACTGGCTCGGAATTACGCCGACGCCGGCAGGAGTGATGTATATACATATACATCACCCGAAGATTCAAACTAATCTACCGCTTCCTATTTAAGAGATTAAACAGGAGAtaata                                                                                                           >  2:3385701/4‑136 (MQ=255)
                                  aTTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAg                                                                                                        >  1:2478971/1‑140 (MQ=255)
                                    ttATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGaa                                                                                                      >  2:2777852/1‑140 (MQ=255)
                                    ttATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAAAAGGAGATCTTTAAGaa                                                                                                      >  2:1796770/1‑140 (MQ=255)
                                    ttATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGAGCTGTATATCCATATACAAGACCCGAACATTCAATCTAATCTCCCGCTTGCGCTTGACGAGATTGAACAGGAGATCTATAAGaa                                                                                                      >  2:1062417/1‑140 (MQ=255)
                                      aTCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAAt                                                                                                    >  1:787857/1‑140 (MQ=255)
                                       tCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAAtt                                                                                                   >  1:449780/1‑140 (MQ=255)
                                           ttACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTaaa                                                                                               >  1:1937255/1‑140 (MQ=255)
                                            tACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTaaaa                                                                                              >  1:3069309/1‑140 (MQ=255)
                                               tCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGa                                                                                           <  2:3900209/140‑1 (MQ=255)
                                               tCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGa                                                                                           <  2:465248/140‑1 (MQ=255)
                                               tCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGa                                                                                           <  2:2997675/140‑1 (MQ=255)
                                               tCATTCAGCCGACTGGCTCGGAAGGAGGGCGACGCCGGCAGGAGTGCTGTCTTTCCATATAGATGACGCGATGATTCAATCTAATCTCCGGCTGGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGa                                                                                           <  1:3276689/140‑1 (MQ=255)
                                                 aTTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTACAATGAAg                                                                                         >  1:81722/1‑140 (MQ=255)
                                                    cAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGc                                                                                      <  1:2922629/140‑1 (MQ=255)
                                                         gACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGct                                                                                 >  1:2818940/1‑140 (MQ=255)
                                                         gACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAAACTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGct                                                                                 >  2:3365443/1‑140 (MQ=255)
                                                             ggCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGctcctc                                                                             >  1:2598457/1‑140 (MQ=255)
                                                             ggCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGctcctc                                                                             >  1:3435058/1‑140 (MQ=255)
                                                               cTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCgg                                                                           <  1:653902/140‑1 (MQ=255)
                                                               cTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCgg                                                                           <  2:2145176/140‑1 (MQ=255)
                                                               cTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCgg                                                                           <  1:3334270/140‑1 (MQ=255)
                                                                tCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCGTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGc                                                                          <  2:1001228/140‑1 (MQ=255)
                                                                    aaTGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATc                                                                      >  1:3680358/1‑140 (MQ=255)
                                                                       gAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAgg                                                                   <  2:514612/140‑1 (MQ=255)
                                                                        aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAATGGCTTGCTCATCGGCGATCAgca                                                                  >  2:3506097/1‑138 (MQ=255)
                                                                        aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGa                                                                  <  1:1162116/140‑1 (MQ=255)
                                                                        aGGGCCACGCCGGCAGGAGTGCTGTATTTCCATATACATTACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGACATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCATCGGCGATCAGGa                                                                  >  2:370790/1‑140 (MQ=255)
                                                                              aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                            >  1:2203752/1‑140 (MQ=255)
                                                                              aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                            >  1:1586679/1‑140 (MQ=255)
                                                                              aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTAAATCTAATCTCCCGCTTGGGCTTGACGAGATTGTACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                            >  2:3547647/1‑140 (MQ=255)
                                                                                     cAGTAGTGCTGTCTTTCCATATACATGACCCGATGATTCTATCTAATCTCCCGATTGGGCTTGACGAGATTGAACATGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCtt                                                     <  2:3134837/140‑1 (MQ=255)
                                                                                     cAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCtt                                                     <  1:2777852/140‑1 (MQ=255)
                                                                                     cAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAACGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCtt                                                     <  1:3923207/140‑1 (MQ=255)
                                                                                       ggAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTAt                                                   <  2:3730151/140‑1 (MQ=255)
                                                                                       ggAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTAt                                                   <  2:2079975/140‑1 (MQ=255)
                                                                                           tGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGAt                                               >  2:737434/1‑140 (MQ=255)
                                                                                           tGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAAATTAAAATGAAGGGCTTGCTCCTCGGCTATCAGGAAGAGGTTCGCCTTATCGaa                                               >  2:2949677/1‑139 (MQ=255)
                                                                                             cTGTATTTCCATATACATGACCCTATGATTCAATCTAATCTCCCGCTTGGGCTTGAAGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                             <  1:3236526/140‑1 (MQ=255)
                                                                                             cTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                             <  2:702234/140‑1 (MQ=255)
                                                                                             cTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                             <  1:501932/140‑1 (MQ=255)
                                                                                             cTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                             >  2:2113711/1‑140 (MQ=255)
                                                                                              tGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACg                                            >  2:1479564/1‑140 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  1:3738201/140‑1 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  2:1754039/140‑1 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  2:3635214/140‑1 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  1:1692625/140‑1 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  2:3246915/140‑1 (MQ=255)
                                                                                               gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                           <  2:810376/140‑1 (MQ=255)
                                                                                                  tttCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATGCGAccc                                        >  1:3573725/1‑140 (MQ=255)
                                                                                                  tttCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGAccc                                        >  1:2859014/1‑140 (MQ=255)
                                                                                                     ccATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAATCGGTTCGCATTATGGATACGACCCTTc                                     >  2:3280626/1‑140 (MQ=255)
                                                                                                     ccATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTc                                     >  2:3865484/1‑140 (MQ=255)
                                                                                                        tataCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCTGTTAGAATTATGGATAAGACCCTTCAag                                  >  2:1954430/1‑140 (MQ=255)
                                                                                                        tataCATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATTAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGATCGCATTATCGATACGACCCTACAag                                  >  2:964141/1‑140 (MQ=255)
                                                                                                        tataCATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAag                                  >  1:87481/1‑140 (MQ=255)
                                                                                                        tataCATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAag                                  >  2:1646777/1‑140 (MQ=255)
                                                                                                        tataCATGACCCGATGATTCAATCTAATCTCACGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATATAAAATGAAGCCCTACCACCTCGGCCATCAGGAAGCGCTTCTCATAATGTATACGACCCTTCAag                                  >  2:2442885/1‑140 (MQ=255)
                                                                                                            cATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                             >  1:4155504/1‑125 (MQ=255)
                                                                                                             aTGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGa                             <  2:3066763/140‑1 (MQ=255)
                                                                                                             aTGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGa                             <  2:2506513/140‑1 (MQ=255)
                                                                                                             aTGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGa                             <  2:3522841/140‑1 (MQ=255)
                                                                                                             aTGACCCGATGATTCAATCTAATCTACCGCTTTGGCTTGACTAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAACGGCTTGCTCCTCGGCGATAAGGAAGCGGTTAGACATATTGATACTAAAATTCAAgagaga                             >  2:2306670/1‑140 (MQ=255)
                                                                                                              tGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGAc                            <  2:1391176/140‑1 (MQ=255)
                                                                                                               gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                           >  1:108208/1‑140 (MQ=255)
                                                                                                               gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                           >  1:2680197/1‑140 (MQ=255)
                                                                                                               gACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                           >  1:1792271/1‑140 (MQ=255)
                                                                                                               gACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                           >  1:1742019/1‑140 (MQ=255)
                                                                                                               gACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                           >  1:1183027/1‑140 (MQ=255)
                                                                                                                 cccGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                         >  2:1675390/1‑140 (MQ=255)
                                                                                                                 cccGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGATCCTCGGCGAACAGGAAGAGGTTCGCCTTATGGATACGACCATACAAGAGGGACgga                         >  2:2574769/1‑139 (MQ=255)
                                                                                                                  ccGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATCAAGGGCTTGCACCTCGGCGATCAGGAAGCGGTACTCCTTATGGATAAGAACCTTCAAGAGTGACGGTc                        >  2:3962432/1‑140 (MQ=255)
                                                                                                                    gatgatTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATTGATAAGACAATTAAAGAGGGACGGTCaa                      >  2:2874131/1‑140 (MQ=255)
                                                                                                                    gatgatTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGACTTATGGATACGACCATTCAAGATGGACGGACaa                      >  2:1703960/1‑140 (MQ=255)
                                                                                                                     atgatTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGaa                                                                 <  1:143072/96‑1 (MQ=255)
                                                                                                                     atgatTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                         <  1:982184/136‑1 (MQ=255)
                                                                                                                      tgatTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAat                    >  1:83648/1‑140 (MQ=255)
                                                                                                                           cAATATAATCTCCCGCTTGGTCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATatcat               <  1:76744/140‑1 (MQ=255)
                                                                                                                                    ctcCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCggg      <  2:2598457/140‑1 (MQ=255)
                                                                                                                                        cGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCTTg  >  1:1229542/1‑140 (MQ=255)
                                                                                                                                        cGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAAAGCGGGCTTg  >  2:4100349/1‑140 (MQ=255)
                                                                                                                                        cGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTACAAGAGGGACGGTCAAATATCATCAATGCGGGCTTg  >  2:4266669/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TACGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCTTG  >  NZ_CP034943/1112831‑1113106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: