Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,056,112 G→T 5.0% intergenic (‑208/+32) EO946_RS10315 ← / ← EO946_RS10320 CPBP family intramembrane metalloprotease/glutamine amidotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,056,1120GT 5.0% 603.8 / 2.9 224intergenic (‑208/+32)EO946_RS10315/EO946_RS10320CPBP family intramembrane metalloprotease/glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base G (85/120);  new base T (17/2);  total (102/122)
Fisher's exact test for biased strand distribution p-value = 5.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AACTTTATTATCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATACATCTAATTTTTT  >  NZ_CP034943/2055973‑2056251
                                                                                                                                           |                                                                                                                                            
aaCTTTATTATCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACCTATTCCATCATTCAAGGTTATTTAGAATTTAACAAAAAAATg                                                                                                                                              >  2:2921249/1‑140 (MQ=255)
aaCTTTATTATCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATg                                                                                                                                              >  1:815041/1‑140 (MQ=255)
  cTTTATTATCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGcc                                                                                                                                            <  1:429425/140‑1 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAATGTTATTTAGACTTTAACAAAAAAATTCCCCt                                                                                                                                         >  1:1011428/1‑140 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  1:4130670/140‑1 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  2:2024536/140‑1 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  2:473212/140‑1 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  1:1713917/140‑1 (MQ=255)
     tattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATAATCCAACATTCAAGGTTATATAAACTTTAACAAAAAAATGCCCCt                                                                                                                                         >  2:1979899/1‑140 (MQ=255)
     tattatCACAATACGTTCGAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  1:2596927/140‑1 (MQ=255)
     gattatCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCt                                                                                                                                         <  1:1370890/139‑1 (MQ=255)
            acaATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCa                                                                                                                                  >  2:526543/1‑140 (MQ=255)
             caATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCCAGGTTCTTTTGTCTTTAACAAAAAAATGCCCCTTAGTTGAg                                                                                                                                 >  1:1649896/1‑140 (MQ=255)
             caATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTAAAGGTTATTTATTCTTTAAGAAAAAAATTCCCCTTAGTTCAg                                                                                                                                 >  1:3488456/1‑140 (MQ=255)
                   gTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCAt                                                                                                                          <  1:614555/140‑1 (MQ=255)
                    ttCTAGATGCTGTGATTGTTTCAAATTTCTGCATTTCTCTTCTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACTTAGTCCATCATTCAAGGTTATTTAGACTTTAACAATATAATGCCCCTTAGTTCAGGGGCAtt                                                                                                                         <  2:4202581/140‑1 (MQ=255)
                     tCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATg                                                                                                                                              <  1:2485193/119‑1 (MQ=255)
                     tCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCAttt                                                                                                                        >  2:2339999/1‑140 (MQ=255)
                     tCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAAAAAAAAAATGCCCCTTAGTTCAGGGGCAttt                                                                                                                        >  2:4258388/1‑140 (MQ=255)
                       tAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTgg                                                                                                                      >  2:2670233/1‑140 (MQ=255)
                       tAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATAATTAAATGTTATTTAGACTTTAAAAAAAAAATGACACTTAGTTGAGGGGGATTTgg                                                                                                                      >  2:2159382/1‑140 (MQ=255)
                        aGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGGTCAGGGTCATTTGGt                                                                                                                     >  2:404611/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATATACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGAt                                                                                                               >  2:3361892/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTATACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGAt                                                                                                               >  2:830313/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGAt                                                                                                               >  2:3595864/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACGTTAACAAAAAAATGCCCCTTAGTTCATGGGCATTTGGTAATGAt                                                                                                               >  2:3652118/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTAAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTAAGGGGCATTTGGTAATGAt                                                                                                               >  2:3697747/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCAATCAAGGTTATTTATACTTTAAAAAAAAAATTCCCCTTAGTTCAGGGGCATTTGGTAATTAt                                                                                                               >  1:2286965/1‑140 (MQ=255)
                              tgtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTATTTTTTAATAAATAGACAAAATCCATCATTAAAGGTTAATTATACTTTAACAAAAAAATGCCCCGAAGTTCAGCGGCATTTGGTAATGAt                                                                                                               >  2:1647627/1‑140 (MQ=255)
                               gtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAAGAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGAtt                                                                                                              >  2:4170296/1‑140 (MQ=255)
                               gtgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAAAAGACATATTCCAACATTAAAGGTTATTTAGACTTTAAAAAAAAAATGACCCTTAGTTCAGGGGCATTTGGTAattatt                                                                                                              >  1:2244693/1‑140 (MQ=255)
                                tgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATTCCCCTTAGTTCAGGGGCAGTTGGTAATGATTc                                                                                                             >  2:3537696/1‑140 (MQ=255)
                                tgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTc                                                                                                             <  1:694064/140‑1 (MQ=255)
                                tgATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCGTAGTTCAGGGGCATTTGGTAATGAtta                                                                                                             >  2:2726483/1‑139 (MQ=255)
                                  aTTGTTTCAAATTTCGGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTa                                                                                                           <  2:2983735/140‑1 (MQ=255)
                                   ttGTTTCAAATTTCTTCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATACATAGACATCTTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGGTCCGGGGCATTTGGTAATGATTCTAt                                                                                                          <  2:634335/140‑1 (MQ=255)
                                     gTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCCGGGGCATTTGGTACTGATTCTATcc                                                                                                        >  2:2853795/1‑140 (MQ=255)
                                     gTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTATTTTTTAATTAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAAGCATTCTATcc                                                                                                        <  2:240296/140‑1 (MQ=255)
                                      tttCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCa                                                                                                       <  2:152439/140‑1 (MQ=255)
                                      tttCAAATTTCTGCATGACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCa                                                                                                       <  2:3012842/140‑1 (MQ=255)
                                       ttCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATTCCCCTTAGTTCAGGGTCATTTTGTAATGATTCTATCCAt                                                                                                      >  2:881018/1‑140 (MQ=255)
                                          aaaTTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTa                                                                                                   <  1:3404224/140‑1 (MQ=255)
                                                       aCTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTaa                                                                                      <  2:3041265/140‑1 (MQ=255)
                                                                cAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCgaagaa                                                                             <  2:3918268/140‑1 (MQ=255)
                                                                cAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCgaagaa                                                                             <  2:815041/140‑1 (MQ=255)
                                                                  gTTGCGTCGTTTTGTTTTTTAATAAATAGACATATTCCATCATTAAAGGGTATTTAGACGTTAACAAAAAAATGCCCCTTATTTAAGGGGCATTTTGTAATTATTATATCCATTTATTTTATTCATTAAATAGAAGAAGt                                                                           >  1:1439471/1‑140 (MQ=255)
                                                                  gTTGCGTCGTTTTCTTTTTTAATAAATAGACAAAATACATCATTCAAGGTTATTTAGAAATTAAAAAAAAAATGCACCTTCGTTGAGGGGCATTTGGTAAATATTATATCCATTTATTGTATTCATTAAATCGAAGAAGt                                                                           >  1:2913235/1‑140 (MQ=255)
                                                                   ttGCGTCGTTTTCTTTTTTAATAAATATACAAATTCCATCATTAAAGGTTATTTAGACTTTAACAAAAAAATTCCCCTTAGTTAAGGGGCAATTGGTAATGATTATATGCAATTATTTTATTAATTAAATCGAAGAAGTa                                                                          >  1:12841/1‑140 (MQ=255)
                                                                   ttGCGTCGTTTTCTTTTTTAATAAAAAGACATATTCCATCAATAAAGGTTATTTAGACTTTAAAAAAAAAAATACCCTTATTTAAGGGGCATTTGGTAATGATTGTATGCATTTATTTTATTAATTAAATCgaagacttt                                                                          >  1:2925408/1‑136 (MQ=255)
                                                                   ttGCGTCGTTTTCTTTTGTAATAAATAGACATAATCCATCAGTCAAGGTTACTTAGACTTTAACAAAAAAATGCCCCATAGTTCCCGGGCAATGGGTAATGCTTCGATCGAATGATTGTCCTCCAGAACTCGCAGAAGTa                                                                          >  2:2453933/1‑140 (MQ=255)
                                                                       gtcgtTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCa                                                                      <  1:3492459/140‑1 (MQ=255)
                                                                           ttttCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTGAGTTCAGGGGCATTTGGTAATGATTCAATCCATTTAATGTATTCATTAACTCGAAGAAGTAGTCAGGtt                                                                  >  2:2052131/1‑140 (MQ=255)
                                                                              tCTTTTTTAATAAATAGACATCTTCCATCATTCAAGGGTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTAt                                                               <  2:1003399/140‑1 (MQ=255)
                                                                                     taataaATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGTtttt                                                        >  2:1124293/1‑140 (MQ=255)
                                                                                       atcaagagagATATTCCATCATTCAAGGTTATTTAGACTTTAAGAATAAAATGACCCTTAGTTGTGGGGGATCTGGGAATGTTTCTATCCCTTTATTGGATTCATTAACTCGACGAAGTATTCAGGTTTATGGGTtttat                                                      <  2:1183585/134‑1 (MQ=255)
                                                                                       ataaatcCACCTAGTCCATCATTCAAGGTTATTTAGACTTTAACAAAAACATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTtttat                                                      <  1:2387040/140‑1 (MQ=255)
                                                                                       ataaataGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTtttat                                                      <  2:1349255/140‑1 (MQ=255)
                                                                                       ataaataGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTtttat                                                      <  2:1373331/140‑1 (MQ=255)
                                                                                       agaaatcGACATATTCCCTCATACAAGGGTATTTAGACTTTCACACAAAAATGCCCCGGAGGTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTtttat                                                      <  1:781133/138‑1 (MQ=255)
                                                                                         aaataGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTtttattt                                                    <  1:1910019/140‑1 (MQ=255)
                                                                                               aCATCTTCCAACATTCAAGGTTATTGAGACATTAACAAAAACATGCCCCTTAGTTCAGGGGCATTTGGTATTGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTta                                              <  1:3952005/140‑1 (MQ=255)
                                                                                                cATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTtat                                             <  1:3653577/140‑1 (MQ=255)
                                                                                                cATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTtat                                             <  1:3361892/140‑1 (MQ=255)
                                                                                                   atTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATGCAGGTTTATGTGTTTTATTTAGCTTATAca                                          >  2:3287110/1‑139 (MQ=255)
                                                                                                    ttCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCAt                                                                                                      <  1:1352257/79‑1 (MQ=255)
                                                                                                    ttCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCa                                         <  1:1137589/140‑1 (MQ=255)
                                                                                                    ttCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCa                                         >  2:1241570/1‑140 (MQ=255)
                                                                                                    ttCCATCATTCAAGGTTATTTAGACTGTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTAGCCATTTATTGTATTCATTAACTCGACGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCa                                         <  1:71450/140‑1 (MQ=255)
                                                                                                     tCCATCATTCAAGGTTATTTAGTCTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCAt                                        <  1:2713325/140‑1 (MQ=255)
                                                                                                     tCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCAt                                        <  1:3577419/140‑1 (MQ=255)
                                                                                                     tCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCAt                                        <  1:2357485/140‑1 (MQ=255)
                                                                                                     tCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCAt                                        <  2:3077434/140‑1 (MQ=255)
                                                                                                        atcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGaa                                     <  2:1707411/140‑1 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCg                                                                                  >  1:2818292/1‑94 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCg                                                                                  <  2:2818292/94‑1 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAAt                                    >  1:3812423/1‑140 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAAt                                    >  1:1417999/1‑140 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAAt                                    >  2:3385957/1‑140 (MQ=255)
                                                                                                         tcatTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAAt                                    >  1:398179/1‑140 (MQ=255)
                                                                                                         tcatTCAAGGTTATGTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCCTGTGGTAATGATTCTATCCATTTATTGTATTCCTTATCTCGAAGAAGTCTTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAAt                                    >  2:3019595/1‑140 (MQ=255)
                                                                                                           atTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGc                                  <  2:3177900/140‑1 (MQ=255)
                                                                                                           atTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGc                                  <  1:4258388/140‑1 (MQ=255)
                                                                                                           atTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCGATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGc                                  <  1:3555316/140‑1 (MQ=255)
                                                                                                            ttCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCATGTGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTCGCTTATACCATGAATGCa                                 <  2:2140101/140‑1 (MQ=255)
                                                                                                                aGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGt                             <  1:677309/140‑1 (MQ=255)
                                                                                                                aGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGt                             <  1:522873/140‑1 (MQ=255)
                                                                                                                 ggTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTa                            >  1:3597854/1‑140 (MQ=255)
                                                                                                                    tATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCt                         <  1:3697747/140‑1 (MQ=255)
                                                                                                                    tATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCt                         <  2:3484654/140‑1 (MQ=255)
                                                                                                                     aTTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTg                        >  2:185461/1‑140 (MQ=255)
                                                                                                                      tttAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTg                        >  1:2096740/1‑140 (MQ=255)
                                                                                                                      tttAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTgg                       >  1:3033236/1‑140 (MQ=255)
                                                                                                                           aCTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTCTCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCaa                  >  2:1731923/1‑140 (MQ=255)
                                                                                                                           aCTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTAt                                                               <  2:3261612/95‑1 (MQ=255)
                                                                                                                           aCTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTAt                                                               >  1:3261612/1‑95 (MQ=255)
                                                                                                                            cTTGAACAAAAAAATGCCCCTTATTTCAGGCGCATTTTGTACTGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTAGTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCaaa                 <  1:3198255/140‑1 (MQ=255)
                                                                                                                               tAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGTTTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATAc              >  1:4072553/1‑140 (MQ=255)
                                                                                                                               tAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATAc              >  2:3086102/1‑140 (MQ=255)
                                                                                                                               tAACAAAAAAATGCCCCTTAGTTCAGGGGCATGTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATAc              >  1:502185/1‑140 (MQ=255)
                                                                                                                                           gCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATACATCCAAtttttt  <  2:2623398/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AACTTTATTATCACAATACGTTCTAGATGCTGTGATTGTTTCAAATTTCTGCATTACTCTACTTCAGTTGCGTCGTTTTCTTTTTTAATAAATAGACATATTCCATCATTCAAGGTTATTTAGACTTTAACAAAAAAATGCCCCTTAGTTCAGGGGCATTTGGTAATGATTCTATCCATTTATTGTATTCATTAACTCGAAGAAGTATTCAGGTTTATGGGTTTTATTTAGCTTATACCATGAATGCAATGTATCTGGTGCAAATACATCTAATTTTTT  >  NZ_CP034943/2055973‑2056251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: