Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,073,387 A→T 5.3% G302G (GGT→GGA EO946_RS10385 ← NTTRR‑F1 domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,073,3870AT 5.3% 506.4 / 10.3 165G302G (GGT→GGAEO946_RS10385NTTRR‑F1 domain
Reads supporting (aligned to +/- strand):  ref base A (83/71);  new base T (3/6);  total (88/77)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTG  >  NZ_CP034943/2073248‑2073526
                                                                                                                                           |                                                                                                                                           
ttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCGGGTGATCCCGTTGCTCCCGTCGAACCAGTGGCTCCACGTGATCCGGTTGGAGCTGTTGGTCCAGTAGCTCCAGTTGCa                                                                                                                                             >  2:1643447/1‑140 (MQ=21)
  gctccAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAcc                                                                                                                                           <  1:1781457/137‑1 (MQ=255)
    gcCAGTTTCCCCGGTGGCACCAGTTGCTCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccag                                                                                                                                         <  2:1100107/140‑1 (MQ=255)
     ccAGTGTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGCTCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGTTCCTGTAGCTCCAGTTGCAccagt                                                                                                                                        <  1:3679853/140‑1 (MQ=255)
              ccGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccagt                                                                                                                               <  2:855126/140‑1 (MQ=255)
                                cccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGt                                                                                                             >  2:2805211/1‑140 (MQ=255)
                                    ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCTCCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                         >  1:484368/1‑140 (MQ=255)
                                    ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCGCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                         >  1:4181737/1‑140 (MQ=255)
                                    ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                         >  1:2111439/1‑140 (MQ=255)
                                    ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                         >  1:3094903/1‑140 (MQ=255)
                                     tgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGAGGTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTTGCTCCAGTTACTCCCGTTgcgc                                                                                                        >  1:898272/1‑140 (MQ=255)
                                       caccagttgctcccggtgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCa                                                                                                      <  2:2743137/140‑1 (MQ=255)
                                       caccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCa                                                                                                      <  2:3890171/140‑1 (MQ=255)
                                       caccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCa                                                                                                      <  2:1436871/140‑1 (MQ=255)
                                       caccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCa                                                                                                      <  2:3673709/140‑1 (MQ=255)
                                            gttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                 >  2:1837/1‑140 (MQ=255)
                                            gttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                 >  2:4000914/1‑140 (MQ=255)
                                            gttgatcccgttgcTCCTGTTGATCCCGGTGCTCCCGTCGCACCCGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                 <  2:3940633/140‑1 (MQ=255)
                                             ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                <  1:1002016/140‑1 (MQ=255)
                                             ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                <  2:3590962/140‑1 (MQ=255)
                                             ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGGTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                <  2:462806/140‑1 (MQ=255)
                                                 tcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCCGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCgg                                                                                            <  2:604582/140‑1 (MQ=255)
                                                 tcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCgg                                                                                            >  1:3035055/1‑140 (MQ=255)
                                                 tcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCgg                                                                                            >  1:3462425/1‑140 (MQ=255)
                                                 tcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCgg                                                                                            >  1:3581542/1‑140 (MQ=255)
                                                  cccgttggTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTTGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGt                                                                                           <  1:2436133/140‑1 (MQ=255)
                                                  cccgttgcTCCTGGTGATCCCGTTGCTCCAGTTGCCCCCGTTGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGCTCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGt                                                                                           <  2:163885/140‑1 (MQ=18)
                                                      ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACcagtggctccagtggctc                                                                                                                          <  1:769978/105‑1 (MQ=255)
                                                      ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCCGTTGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCa                                                                                       >  1:1378064/1‑140 (MQ=255)
                                                      ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCa                                                                                       >  2:896438/1‑140 (MQ=255)
                                                      ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCa                                                                                       >  1:2706593/1‑140 (MQ=255)
                                                      ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGGTGCGCCAGTTTCCCCGGTTGcg                                                                                       >  2:3634926/1‑139 (MQ=255)
                                                           ccTGTTGATCCCTTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCAATTGGACCTTTTGGTCCTGTAGCTCCAGTTGCACCAGTTGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgt                                                                                  <  2:1811399/140‑1 (MQ=25)
                                                           ccTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgt                                                                                  <  2:1965677/140‑1 (MQ=255)
                                                           ccTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCGGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgt                                                                                  >  1:3921879/1‑140 (MQ=255)
                                                               ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                              >  1:1894311/1‑140 (MQ=255)
                                                                    cccGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGCCTCCAGTTGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgt                                                                         >  2:3112178/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCCGTTGCACCACTGGCTCCAGAGGCGCCAGTTACTCCCGTTGCGCCAGTATCACCCGGTGCACCAgttgctcccggtgg                                                                      >  2:340302/1‑139 (MQ=25)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgc                                                                      >  2:1842260/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgc                                                                      >  2:1074447/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgc                                                                      >  1:2299721/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgc                                                                      >  1:671378/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatccagttgc                                                                      >  2:1132739/1‑140 (MQ=255)
                                                                       gTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCAGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTCCTCCCGTTGAGCCAGTTGCCCCGGTTGCACCAggtgataccgttgc                                                                      >  2:283079/1‑140 (MQ=25)
                                                                                     aCCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccg                                                        >  2:4034331/1‑140 (MQ=255)
                                                                                     aCCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccg                                                        >  2:4147171/1‑140 (MQ=255)
                                                                                     aCCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGCTCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccg                                                        >  2:755389/1‑140 (MQ=255)
                                                                                         gTGGCTGCTGTTGATCCCGTTGTTCCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttgc                                                    <  2:350806/140‑1 (MQ=255)
                                                                                          tGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttgct                                                   >  1:791551/1‑140 (MQ=255)
                                                                                               ccTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTtgatcccgttgctcctgttgatcccgttgctcctgt                                              >  1:826362/1‑140 (MQ=255)
                                                                                               ccTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTtgatcccgttgctcctgttgatcccgttgctcctgt                                              >  1:3942345/1‑140 (MQ=255)
                                                                                                   ttGCTCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTGACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATcccgttgctcctgttgatcccgttgctcctgttgat                                          <  2:2680246/140‑1 (MQ=255)
                                                                                                   ccgaccccGTTGCACCCGTCGGCCCTGTAGCACCACGTGCACCAGTCCCTCCTCTGCCGCCAGTGACTCCCCTTCCGCCAGTTTCCCCGGTTGCACCAGTTGATcccgttgctcctgttgatcccgttgctcctgttgat                                          <  2:2673854/135‑1 (MQ=14)
                                                                                                      aTCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCgttgcgcctgttgatcccgttccgcctgttgatccc                                       >  1:417283/1‑140 (MQ=255)
                                                                                                        cccTTTGGACCTGTTTGTCCTGTAGCTCCAGTTGCACGAGTGGCTCCATTGGCTCGTGTTACTCCCGTTGCGCGAGTTTCCCCGGTTGCACCAGTTGATcccgttgctgtattttatacagttgctcctgttgatgagtt                                     <  2:340339/140‑6 (MQ=17)
                                                                                                        cccGTTGGAGCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGATCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                         <  1:3302102/72‑1 (MQ=255)
                                                                                                        cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                     >  1:2490221/1‑140 (MQ=255)
                                                                                                        cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                     >  2:1306386/1‑140 (MQ=255)
                                                                                                          cGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCCGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCGGTTGATCCCGTCg                                   >  2:1874576/1‑140 (MQ=255)
                                                                                                          cGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCACCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCg                                   >  2:2947681/1‑140 (MQ=255)
                                                                                                           gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGc                                  >  1:1584157/1‑140 (MQ=255)
                                                                                                            ttGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCa                                 >  1:575619/1‑140 (MQ=255)
                                                                                                             tGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAc                                >  1:1011433/1‑140 (MQ=255)
                                                                                                              ggACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAcc                               <  2:484926/140‑1 (MQ=255)
                                                                                                              ggACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAcc                               <  2:4181737/140‑1 (MQ=255)
                                                                                                              ggACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAcc                               <  2:1679557/140‑1 (MQ=255)
                                                                                                              ggACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAcc                               <  2:2111439/140‑1 (MQ=255)
                                                                                                                    gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGa                         >  2:264212/1‑140 (MQ=255)
                                                                                                                    gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGa                         >  2:4082705/1‑140 (MQ=255)
                                                                                                                    gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGa                         >  2:1329756/1‑140 (MQ=255)
                                                                                                                    gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGa                         <  2:3094903/140‑1 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGGCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        <  1:3634926/140‑1 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        <  1:1306386/140‑1 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        <  1:1983278/140‑1 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        >  2:2666641/1‑140 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        >  1:28115/1‑140 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        >  1:4119973/1‑140 (MQ=255)
                                                                                                                     ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGAACCTGTTTAt                        >  2:3271772/1‑140 (MQ=255)
                                                                                                                     ttGATCCCGTAGCTCCTGTTGGTCCAGTTGCTCCCGTTTATCCAGTTTCTCCCGTTGCGACAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        <  2:2461326/140‑1 (MQ=17)
                                                                                                                          ccTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGt                   >  1:1988408/1‑140 (MQ=255)
                                                                                                                          ccTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGt                   <  1:264212/140‑1 (MQ=255)
                                                                                                                          ccTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGt                   >  1:3794028/1‑140 (MQ=255)
                                                                                                                          ccTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCACTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTTATCCCGTTGCTCCGGTTGATCCCGTCGCACCTGTTGATCCCGt                   >  1:513298/1‑140 (MQ=255)
                                                                                                                            tGTAGCTCCAGTTGCACCAGTGGCTCCAGTTGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTg                 <  2:1481662/140‑1 (MQ=255)
                                                                                                                             gTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTgg                <  2:791551/140‑1 (MQ=255)
                                                                                                                             gTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCGGTTGATCCCGTTgg                >  2:925795/1‑140 (MQ=255)
                                                                                                                              tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa               >  2:584944/1‑140 (MQ=255)
                                                                                                                              tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa               >  2:3060207/1‑140 (MQ=255)
                                                                                                                                 cTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCt            <  1:3346918/140‑1 (MQ=255)
                                                                                                                                   ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        >  1:4017599/1‑126 (MQ=255)
                                                                                                                                   ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                        <  2:4017599/126‑1 (MQ=255)
                                                                                                                                   ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt          >  2:3949872/1‑140 (MQ=255)
                                                                                                                                   ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt          <  1:4254850/140‑1 (MQ=255)
                                                                                                                                   ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt          <  2:3617836/140‑1 (MQ=255)
                                                                                                                                      gTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCAGTTGCGCCAGTTTCCCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGTCGTGTTgg       >  2:2225737/1‑140 (MQ=255)
                                                                                                                                          cACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCGGTTGATCCAGTGGCTCCTGTTGATCCAgttggtcctgttggtcct   >  2:1724814/1‑140 (MQ=14)
                                                                                                                                           aCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTg  >  2:1217008/1‑140 (MQ=255)
                                                                                                                                           aCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTg  >  2:1706625/1‑140 (MQ=255)
                                                                                                                                           aCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTACACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTg  >  2:3350185/1‑140 (MQ=255)
                                                                                                                                           aCCAGCGCCTCCAGGTGCTCCAGTTTCGCCGGGTGCCCCAGTTGCGCCTGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTg  <  2:909343/140‑1 (MQ=14)
                                                                                                                                           |                                                                                                                                           
TTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTG  >  NZ_CP034943/2073248‑2073526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: