Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,103,816 C→A 8.5% intergenic (‑33/+118) azoJ ← / ← EO946_RS10560 FMN‑dependent NADH‑azoreductase AzoJ/TraR/DksA family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,103,8160CA 8.5% 345.1 / 2.1 143intergenic (‑33/+118)azoJ/EO946_RS10560FMN‑dependent NADH‑azoreductase AzoJ/TraR/DksA family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (74/49);  new base A (16/2);  total (92/51)
Fisher's exact test for biased strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAC  >  NZ_CP034943/2103680‑2103953
                                                                                                                                         |                                                                                                                                          
gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAATGTAGACATTTTTATTCCTCCCGGTAAACTTTTTATATTAAATTa                                                                                                                                         >  2:692160/1‑140 (MQ=255)
    tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                                                                     <  2:1540362/140‑1 (MQ=255)
    tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                                                                     <  1:3492102/140‑1 (MQ=255)
    tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                                                                     <  1:1884820/140‑1 (MQ=255)
     aTTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAAAAATAATGTAGACAATTTTATTACGCCCGGGAAAATTTTTTTATTAAATTAaaaaa                                                                                                                                    >  1:2046258/1‑139 (MQ=255)
      ttttCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGATTAGTTGAATCGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATa                                                                                                                                   <  1:1864696/140‑1 (MQ=255)
        ttCATTCTAAGCAGCTAAGAAAGCCTCGTCAAGTTTAGTTGAAACGACTTCTTCGGCTGTACGGTCGATTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGt                                                                                                                                 <  2:2851706/140‑1 (MQ=255)
           tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTCAAATAATACTGGAGACATTTGTATTGCTGCCGGTAAACTTTTTATATTTACTTACCAATAGTATg                                                                                                                              >  2:3657695/1‑140 (MQ=255)
           tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATg                                                                                                                              <  1:2602895/140‑1 (MQ=255)
           tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACAGGAGACATTTGTATTACTCCCGGTAAACTTTTTATATTAACTTACAAATAGTAtt                                                                                                                              >  2:3952927/1‑139 (MQ=255)
           tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATACAGTAGACATTTGTATTACTCCCGGTAAACTTTTTATATTAACTTAAAAATAGTAtt                                                                                                                              >  2:2698854/1‑139 (MQ=255)
           tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATg                                                                                                                              >  2:1059536/1‑140 (MQ=255)
             tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTGTTTAAAAATAATACTGTTGAGATTTTTTTTGCTCGCGGTGAACTTTGTTTATTAAGATAGAAATAGTAttgt                                                                                                                            >  1:3793433/1‑137 (MQ=255)
             tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTACAAATAAAAATGTAGACATTTTTTTTGCTGCCCGTAAACAGTTTATATTAACTTAAAAAAAGTAtttt                                                                                                                            >  1:1835138/1‑140 (MQ=255)
             tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTAAAAATAATAATTTATACATTTTTATTGCTCCCCGTAAAATTTTTATATTAACTTAAAAATATTATGtt                                                                                                                            >  1:2677277/1‑140 (MQ=255)
             tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTAAAAATAATAATGTAGACATTTTTATTACTCCCGGTAAAAGTTTTATATTAAATTAAAAATATTAtttt                                                                                                                            >  1:2404359/1‑140 (MQ=255)
                     gcTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTGCTCCCGGTAAACTTTTTTTATTAAATTAAAAATAGTATGTTActttgtt                                                                                                                    >  2:3518787/1‑140 (MQ=255)
                       tAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAATAAAAATTTAGGAAATTTTATTGCTGCCCGGTTACTTTTTATATTTAATTTAAAATAGTATGTTTGTTTAtttt                                                                                                                  >  1:3733027/1‑140 (MQ=255)
                       tAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTACAAATAATACTGTAGACCATTTTATTTCTCCCGGTAAACGTTTTATATTAACTTACAAATATGATTTTACTTTCtttt                                                                                                                  >  1:762143/1‑140 (MQ=255)
                          gAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGGTTTTACAAATAATAAAGTATACATTTGTATTCCGCGCGGTAAAGTTTTTAAAATAAAATAAAAATATTATGTTAATTTGTTTTTTa                                                                                                               >  2:1304786/1‑140 (MQ=255)
                          gAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAAAACGGTAGACATTTTTATTGCGGCCGGGACACGTTTTATCATAACTTACAAAAAGTATGTTACTTTCTTTTTTa                                                                                                               >  2:233970/1‑140 (MQ=255)
                           aaaGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTAAAAATAAAACTGTAGACAATTTTATTCCTCCCGGTAAAAATTTTATATTAACATAAAAATAGTATTTTAATTTATTTTTTaa                                                                                                              >  2:3778264/1‑139 (MQ=255)
                            aaGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACg                                                                                                             <  1:2866545/140‑1 (MQ=255)
                              gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGAGTTTAAAAATAATACAGTAGACAATTGTATTACTCCCGCGAAACGTTTTATATTAAAATAAAAATAGTATGTTACTTTCTTTTTTAAGCa                                                                                                           >  2:2555565/1‑140 (MQ=255)
                                   gTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTc                                                                                                      <  2:127916/140‑1 (MQ=255)
                                     aatgTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGAGTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCAGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCaa                                                                                                    <  1:2948121/137‑1 (MQ=255)
                                     aaaGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCaa                                                                                                    <  1:2049423/140‑1 (MQ=255)
                                     aaaGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCaa                                                                                                    <  1:1365257/140‑1 (MQ=255)
                                              ttGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTTAAAATAATAATGTATAAATTTTTTTTCCTCCCGGTAAACTTTTTATAGTAACTTACAAAAACTATTTTAATTTATTTTTTAATCAAAGTCAATCACTTTAt                                                                                           >  2:4074970/1‑140 (MQ=255)
                                              ttGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTAAAAATAATACTTTAGACATTTTTATTCCTCCCGGGAAACTTTTTATATTAAATTAAAAATAATATTTTAATTTGTTTTTTAAGCAAAATAAATAACTTTAt                                                                                           >  2:211180/1‑140 (MQ=255)
                                              ttGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTACAAATAATACTGTAGACAATTGTATTCCTCCCGGTAAACATTTTATATTAACTTACAAATAGTATGTTACTTTCATTTTTTAGCAAAGTAAATCACTTTAt                                                                                           >  2:4136278/1‑140 (MQ=255)
                                              ttGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTTGAAAAAATAATGTAGACCTTTGTATTCCGCCCGGGAAACTTTTTATATTAAATTACAAATAGTATGTTTGTTTGTTTTTTTCGCAAAGTCAATCAATTTAt                                                                                           >  1:4071959/1‑140 (MQ=255)
                                               tGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTAtt                                                                                          <  2:3747066/140‑1 (MQ=255)
                                                 aaaCGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATCCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTAtttt                                                                                        <  2:3731320/140‑1 (MQ=255)
                                                   aCGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTAAAAAAAAAATTTATACATTTTTATTACAACCGGTAAAAATTTTATATTAAATTAAAAAAAGTATTTTAATTTATTTTTTAAGCAAAGTAAATAACATTAttttta                                                                                      >  2:689610/1‑138 (MQ=255)
                                                   aCGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATAATGTAGACATTTGTATTCCTCCCGGTTAACTTTTTATATTAACTTAAAAATAGTATGTTACTTTATTTTTTAAGCAAAGTCAATAACGTTATTTTGa                                                                                      >  2:444808/1‑140 (MQ=255)
                                                   aCGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTTAAAATAAAACTGTATACCTTTGTTTTACTGCCGGTAAACTGTTTTTATTAAATTAAAAAAAGTATGTTTCTTTTTTTTTTTGGGAAAATGAATAACTTTAtttttt                                                                                      >  1:84120/1‑138 (MQ=255)
                                                                   tACGGTCGCTTGTTTTTAAAAAAAATAATGTAGACAATTGTATTCCTCCCGGGAAACTTTTTATATTAACTTACAAATAGTATGTTAATTTCTTTTTTAAGCAAAGTCAAAAAATTTATTTTTAAAATGTTAtttttttt                                                                      >  2:3505335/1‑140 (MQ=255)
                                                                   tACGGTCGCGTGTTTTTAAAAAAAATACGGTAGACATTTTTATTGCTCCCGGGAAAATTTTTATATTAAATTAAAAAAATTATGTTAAATTATTTTTTAAGCAAAATCAATCAATTTATTTTGAATATGAGAtttttttt                                                                      >  2:502012/1‑140 (MQ=255)
                                                                   tACGGTCGCGTGATTTTACAAATAATACTGTAGACAATTGTATTCCTCCCCGTAAACTTTTTATATTAAATTACAAATAGTATGTTACTTTATTTTTTTAGCAAAGTCAATCACTTTATTTTGAATATTAGAtttttttt                                                                      >  2:2333215/1‑140 (MQ=255)
                                                                        tCGCTTGATTTTAAAAATAAAAATGTAGACATTTTTATTGCTCCCGGTAAACTTTTTATATTAAAATAAAAATAGTATGTTACTTTCTTTTTTACGCAAAATAAATCACTTTATTTTGAATATTAGATTTTTTTTAaaaa                                                                >  2:1927637/1‑140 (MQ=255)
                                                                        tCGCGTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTAAAAATATTATTTTACTTTATTTTTTAAGCAAAGTAAAACACATTATTTTTAATAAGAGATTTTTTTTCaaaa                                                                >  2:4211443/1‑140 (MQ=255)
                                                                          gcgtGAATTTAAAAATAATAAAGTAGACAATTTTATTACTCCCGGTAAACTTTTTATATTAACTTACAAAAATTATTTTACTTTATTTTTTACGCAAAGTCAAACACTTTATTTTTAATATGTAAATTTTTTTAaaaaaa                                                              >  2:2557645/4‑140 (MQ=255)
                                                                          gCTTGATTTTACAAATAATAATGTAGACATTTGTATTACTCCCGGTAAAATTTTTATATTAACTTACAAATATTATTTTAATTTCTTTTTTAAGCAAAGTCAATCACTTTATTTTTAATATTAGATTTTTTTTGaaaaaa                                                              >  2:408501/1‑140 (MQ=255)
                                                                           cTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATACTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaaaa                                                             <  1:2815721/140‑1 (MQ=255)
                                                                             tgcttttAAAAATAATAATTTAGACATTTGTATTCCTCCCCGTAAACTTTTTATATTAACTTACAAATATTATTTTACTTTATTTTTTAAGCAAATTAAATCAATTTAATTTTAATATTATATTTTTTTTAaaaaaaaaa                                                           >  2:2181983/4‑139 (MQ=255)
                                                                                 tttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAg                                                       <  1:1186916/140‑1 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCaatt                                                      >  2:3029383/1‑138 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt                                                      <  2:3790835/140‑1 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt                                                      >  2:735439/1‑140 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt                                                      <  1:692160/140‑1 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt                                                      <  2:608127/140‑1 (MQ=255)
                                                                                  ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt                                                      <  1:508273/140‑1 (MQ=255)
                                                                                   tACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTa                                                     <  2:3626958/140‑1 (MQ=255)
                                                                                   tACAAATAATACTGTAGACATTTGTAGTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTa                                                     <  2:2010271/140‑1 (MQ=255)
                                                                                     aaaaTAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACa                                                   <  1:1059536/139‑1 (MQ=255)
                                                                                          aataCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTaa                                              <  2:3300788/140‑1 (MQ=255)
                                                                                             aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat                                           <  2:173061/140‑1 (MQ=255)
                                                                                             aCTCTAGACATTTGTAGTCCTCCCGGTAACCTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat                                           <  1:762483/140‑1 (MQ=255)
                                                                                              cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAAGCAAGTACATCTAatat                                           <  2:2440963/140‑1 (MQ=255)
                                                                                                gTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCgg                                        >  2:228945/1‑140 (MQ=255)
                                                                                                 tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATTTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTAAAATAAAAGCAAGTACATCTAATATCGgt                                       >  2:2059316/1‑140 (MQ=255)
                                                                                                 tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGgt                                       >  1:2997522/1‑140 (MQ=255)
                                                                                                 tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGgt                                       >  1:1551475/1‑140 (MQ=255)
                                                                                                   gACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTAGTTTCTTTTTTACGCAAAGTCAATCACTTTTTTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGgtgt                                     >  2:3480814/1‑140 (MQ=255)
                                                                                                   gACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGgggt                                     >  1:4000682/1‑140 (MQ=255)
                                                                                                   gACATTTGTATTCCTCCCGGTAAACGTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAAGCAAGTACATCTAATGTCGgtg                                      >  2:532299/1‑140 (MQ=255)
                                                                                                    aaaTTTGTATTCCTCCCGGTAAACATTTTATATTAAATTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTa                                    <  1:4074970/138‑1 (MQ=255)
                                                                                                    aCATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTa                                    <  1:4136278/140‑1 (MQ=255)
                                                                                                      aTTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTAGGCCAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTAct                                  >  2:2618911/1‑140 (MQ=255)
                                                                                                      aTTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTAct                                  >  2:4085190/1‑140 (MQ=255)
                                                                                                      aTTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTAct                                  >  2:3459806/1‑140 (MQ=255)
                                                                                                      aTTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTAAATCTAATATCGGTGTAct                                  >  2:2662501/1‑140 (MQ=255)
                                                                                                         tGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTActtgc                               >  2:3414581/1‑140 (MQ=255)
                                                                                                         tGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTActtgc                               <  2:531162/140‑1 (MQ=255)
                                                                                                         tGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTActtgc                               >  2:582576/1‑140 (MQ=255)
                                                                                                         tGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTActtgc                               >  2:1569609/1‑140 (MQ=255)
                                                                                                                ctccCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAt                        >  2:318573/1‑140 (MQ=255)
                                                                                                                ctccCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAt                        >  1:1395747/1‑140 (MQ=255)
                                                                                                                ctccCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAt                        >  1:3117695/1‑140 (MQ=255)
                                                                                                                ctccCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAt                        >  2:19382/1‑140 (MQ=255)
                                                                                                                ctccCGGTAAACTATTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACCCAAAGTCAATCACTTTATTTTGAATATGATATTTTTTTTAAAAAAAAAGCAATTAAATATAATATCGTTGTACTTGCTTGCCaa                        >  2:1626556/1‑139 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATGTAATATCGGTGGACTTGCTGGCCAttt                      >  2:566475/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  2:3813191/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  2:1503518/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  1:2237915/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  1:1195093/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  1:637757/1‑140 (MQ=255)
                                                                                                                   cccGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGAAAGTACATCTAATATCGGTGTACTTGCTTGCCAttt                      >  1:3433474/1‑140 (MQ=255)
                                                                                                                      ggTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAATGCAAGTACAGCTAATATCGGTGTACTTGCTTGCCATTTTCa                   >  1:2360999/1‑140 (MQ=255)
                                                                                                                      ggTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATAGGTGTACTTGCTTGCCATTTTCa                   >  2:3741181/1‑140 (MQ=255)
                                                                                                                           aCTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACttag              >  1:154887/1‑138 (MQ=255)
                                                                                                                           aCTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCg              >  1:810847/1‑140 (MQ=255)
                                                                                                                               tttATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATc          <  1:2772781/140‑1 (MQ=255)
                                                                                                                                ttATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATc          <  1:2383198/140‑1 (MQ=255)
                                                                                                                                      tAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCa   <  2:2404359/140‑1 (MQ=255)
                                                                                                                                       aaCTTACAAATTGTATGTTACTTTCTTTTTTCCGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAc  <  1:3131057/140‑1 (MQ=255)
                                                                                                                                       aaCTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAc  <  1:2333215/140‑1 (MQ=255)
                                                                                                                                       aaCTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAc  >  2:737135/1‑140 (MQ=255)
                                                                                                                                       aaCTTACAAATAGTATGTTACTTTCTTTTATACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAc  <  1:4120345/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGCCATTTTCACTTCGAATCTTGTTCAC  >  NZ_CP034943/2103680‑2103953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: