Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,480 A→C 8.0% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4800AC 8.0% 716.9 / 8.1 289A119A (GCT→GCGdeoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (187/65);  new base C (2/35);  total (189/100)
Fisher's exact test for biased strand distribution p-value = 2.68e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTC  >  NZ_CP034943/2139343‑2139614
                                                                                                                                         |                                                                                                                                      
  cGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGCCATCATCGGCATTTTTTAAAAGCTCGAAATCGGCGCACGGCGCAAAAACAATACTTCCCAACGCGa                                                                                                                                    <  2:525511/140‑1 (MQ=255)
    aaTCGTGTTTTTAGAACTGATCAGCTGTAAATAGGCTTCCGACCGTCACCGGCACGCTTTTATCCTTGGCGGAATCATTGGAATTTTTTAAAAGCTCGAAATCTGGGAATGGCGCAAAATCACTAGTTCCGAAAGCGAct                                                                                                                                  <  2:1417368/140‑1 (MQ=255)
    aaTCGTGGTTTTATAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCATTTATCCTTTGCGGCATAATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAAACAATACTTCCGAACGCGAct                                                                                                                                  <  2:1913619/140‑1 (MQ=255)
       cgtcgtTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTg                                                                                                                               >  2:1532574/1‑140 (MQ=255)
         tgtttttAGAACTTATCAGCTTTAAATACGCTTCCTCCCGTCCCGGGCACGCCTTTATCCTTTGCGGAAACATAGTAATTTTTTAAAAGCTCCAAATCGGCCCAAGGCCAAAAATCAATACTCCCCAAAGCGACTCTGtt                                                                                                                             <  1:3198966/134‑1 (MQ=255)
          cgtTGTAGACATGATCAGGCGGAAATACGCGACCTACCGTCACGGGCACGCGTTTATCGTTGGCGGAATCATAGGCATTTTTTAAAAGCTCGAACTCTGGGCAAGGCGCAAAATCAAGCGTTCCGAACGCGACTCTGTTc                                                                                                                            <  2:3257272/140‑1 (MQ=255)
                    cTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTGCGAAAGCGACTCTGTTCATTTGTGaa                                                                                                                   <  2:3593587/139‑1 (MQ=255)
                       aTCAGCTGTAAATACGGTTCCTACCGTCACCGGCACGCCGTTATCCTTTGCGGCATCATAGGCATTGTTTAACAGCTCGCAATCTGCGCAAGGCGCAAAATCAATACGTCCGAAAGCGACTCTGTTCATTTGTGACTCAg                                                                                                               >  2:1471001/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  2:712307/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  1:385889/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  1:1640401/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  2:2864899/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  1:3093393/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  2:1616642/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                               >  1:2073522/1‑140 (MQ=255)
                           gCTTTAAATAGGCTTCCTACCGTCACCGGCACGCTTTTATCCTTTGCGGCATAATAGGAATTTTTTAAAAGCACCAAATCTGCGCAAGGCCAAAAAAAAATCATCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGa                                                                                                           <  1:3963558/140‑1 (MQ=255)
                             ttaaaaTACGCTTCCTACCGTCCCGGGCACGCCTTTAGCCTTGGCGCAAACATCGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  1:3287452/137‑1 (MQ=255)
                             tgaaaaTCCGCTGCCTACCGTCACCGGCACGCCTTGATCCTTTCCGGCATCATCCGCATTTTTTAAAAGCTCGAAATCTGCGCACGGCGAAAAAACAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  1:1980959/137‑1 (MQ=255)
                             tGTAAATCCGCTTCCTACCGTCACGGGCACGCTTTTATCGTTTGCGGCATCATCGGATTTTTTTAAAAGCTCGAAATCTGCGCACGGCCAAAAATCAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  1:2323438/140‑1 (MQ=255)
                             tGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  2:3298969/140‑1 (MQ=255)
                             tGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  2:4089121/140‑1 (MQ=255)
                             tGTAAATACGCTTCCTACCGTCACCGGCACGCATTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAAACTGCGCACGGCGAAAAACCAATACTGCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  2:2215603/140‑1 (MQ=255)
                             tGTAAATACGCTCCCTACCGCCCCCGGCACGCCTTTATCCTTGGCGCCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGAAAGGCGAAAAATCAATACTGCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                         <  1:2901379/140‑1 (MQ=255)
                               tAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAATAGCTCGAGATCTGCGCAAGGCGCAATATCCATACTTCCGAACGCGACTCTGTTCATGTGTGATTCAGTTGAGGAt                                                                                                       >  1:4215137/1‑140 (MQ=255)
                                 aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGTATCAGTTGAGGATGt                                                                                                     >  1:3157550/1‑140 (MQ=255)
                                 aaTACGCTTCCTACCGTCACCCGCACGCCTTTATCCTTTCCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGAATCAGTTGAGGATgg                                                                                                     >  1:2206868/1‑139 (MQ=255)
                                  aTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTc                                                                                                    <  2:516063/140‑1 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCTTAGGGATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  2:2444680/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:1175089/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  2:2524255/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:4041298/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  2:3578096/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:3484432/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:1818588/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:277938/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGAATCAGTTGAGGATGTCATAg                                                                                                >  1:934432/1‑140 (MQ=255)
                                       cTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGc                                                                                               >  2:1526050/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                             >  1:1542174/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                             >  2:3471347/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                             >  1:1901478/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                             >  2:2041224/1‑140 (MQ=255)
                                          ccTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCaa                                                                                            >  2:3955528/1‑140 (MQ=255)
                                          ccTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGGTCATTTGTGAATCAGTTGAGGATGTCATAGCCaa                                                                                            >  2:1571983/1‑140 (MQ=255)
                                          ccTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTGTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCaa                                                                                            >  2:26825/1‑140 (MQ=255)
                                           cTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCaaa                                                                                           >  2:3366585/1‑140 (MQ=255)
                                              ccGTCACCGGCACGCCTTGATCCTTTGCGGCAACATAGGCATTTTTTAAAAGCACGAAAGCTGCCAAAGGCGCAAAATCAATACTCCCGACCGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATg                                                                                        <  2:2668400/140‑1 (MQ=255)
                                              ccGCCACCGGCACGCCTTTATCCTTTGCGCCATCATAGGAATTTTTTAAAAGCTCGAAATCGCCGCAAGGCGAAAAATCAATACTACCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATg                                                                                        <  1:3249804/140‑1 (MQ=255)
                                                 tCACCGGCAGGCGTTTATCCTTTGCGGCATCATAGGGATTTTTTAAATGGTGGAAATCTGCGCAAGGCGCAAATTCAAGCCTGCCGACAGCGACTGTGTTCATTGGTGACTCTGTTGAGGATGGGATAGGCTAATTGagg                                                                                     >  2:1085092/1‑138 (MQ=255)
                                                 tCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACg                                                                                     >  2:4160636/1‑140 (MQ=255)
                                                      ggCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                <  2:2383624/140‑1 (MQ=255)
                                                      ggCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCGGCGCAAGGCGCAAAATCAATACGTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                <  2:260420/140‑1 (MQ=255)
                                                                tATCCTTTGCGGCATAATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATCCTGCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                      <  2:1290422/140‑1 (MQ=255)
                                                                    cTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGACTCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACCTCtt                                                                  >  1:546640/1‑140 (MQ=255)
                                                                     tttCCGGCATCATCGGCATTTTTTAAAAGCTCGAAATCTGCGCATGGCGAAAAATCAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCttt                                                                 <  1:68764/140‑1 (MQ=255)
                                                                      ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                >  1:2615514/1‑140 (MQ=255)
                                                                      ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                >  1:1611019/1‑140 (MQ=255)
                                                                       ttctccatcatAGGCATATTTTAAAAACTCTAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCTACTCTGTTAATTTTTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:383600/6‑140 (MQ=255)
                                                                       ttctccatcagAGGCATATTTTAAAATCTCTAAATCTGCGCAAGGAGCAAAATCAATACTTACTAAAGCTAATCTGTTAATTTGTGAATCAGTTGAGTATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:253150/6‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCTACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGAAGTCCCGCACTTTGACATCTTTGc                                                               >  1:2730313/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGACTCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:3241150/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                <  2:316114/123‑1 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:2721793/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:4116528/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:1038923/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:1708377/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:504842/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:466556/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:112166/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:4020907/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:1197072/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:1361069/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:3643680/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:1445556/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:3023275/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  1:2284626/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCGGTTCAGTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCCCTTTGACATCTTTGc                                                               >  2:3906755/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGAGCAAGGCGCAAAATCAATACTTCAGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCttttc                                                               >  1:2447493/1‑138 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTAGAAATCTGCGCAAGGCGCAAAATCAATAATTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                               >  2:2095079/1‑140 (MQ=255)
                                                                         cGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCgg                                                             >  2:2202665/1‑140 (MQ=255)
                                                                         cGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCGGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCgg                                                             >  2:3680920/1‑140 (MQ=255)
                                                                                    gaaTTTTTAAAAAGCTCTAAATCTGCGCAAGCCGCAAAATCAATCCTACCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                  <  2:4215137/138‑1 (MQ=255)
                                                                                    gCATTTTTTAAACGCTCTAAATCGGCGCACGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                  <  1:384882/140‑1 (MQ=255)
                                                                                    gCATTTTTTAAACCCTCCAAATCTGCGCAAGGCGCAAAAGCAATACTGCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                  <  1:2343869/140‑1 (MQ=255)
                                                                                        tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                                              >  2:4137539/1‑140 (MQ=255)
                                                                                        tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                                              >  2:828635/1‑140 (MQ=255)
                                                                                        tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                                              >  1:3744590/1‑140 (MQ=255)
                                                                                         ttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAGAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcgc                                                      <  1:3360652/131‑1 (MQ=255)
                                                                                         ttttAAAAGCTCGAAATCTGCGCAAGGCGAAAAATAAATACTCCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAc                                             <  2:493840/140‑1 (MQ=255)
                                                                                            aacaaGCTCCAAATATGCGCACGGCCCAAAATCAAGCCTTCCTAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                                          <  1:2484665/137‑1 (MQ=255)
                                                                                              aaaGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAct                                        <  2:2737370/140‑1 (MQ=255)
                                                                                                           tGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGAtttt                           >  2:362459/1‑140 (MQ=255)
                                                                                                           tGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGAtttt                           >  2:1848544/1‑140 (MQ=255)
                                                                                                           tGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGAtttt                           >  1:1567435/1‑140 (MQ=255)
                                                                                                            gcgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTg                          >  1:359360/1‑140 (MQ=255)
                                                                                                            gcgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTg                          >  1:1043946/1‑140 (MQ=255)
                                                                                                              gcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                        <  2:3093393/140‑1 (MQ=255)
                                                                                                              gcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                        <  2:3065736/140‑1 (MQ=255)
                                                                                                                     gcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTa                 >  1:664279/1‑140 (MQ=255)
                                                                                                                         aaTCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCtt             <  2:466556/140‑1 (MQ=255)
                                                                                                                                    ccGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattc  >  1:2014476/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTC  >  NZ_CP034943/2139343‑2139614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: